FvH4_1g03760

Early nodulin-93-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0003255
Physical Location & Seq
Reverse (-)
2011258 .. 2012675
1418 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g03760.t1

Sequence Viewer

Length: 318 bp
ATGGCAAAGAATGTGTCTCAATCTCCCATGGAGAAACACAGCCTGACTTCATTTGACCAGCAGTTGGCTAAGCGCTGTTCTCATGAGGGTGTAAAAGCTGGAGTTAAGGCAGCTGTTGTTGCTACCATTGCCAGTGCAATTCCAACTTTTGCTAGTGTGAGAATGCTGCCTTGGGCAAAAGCCAATCTGAATCCCACTGCTCAGGCTCTCATAATCTCAACAGCGGCTGGAATGGCATATTTCATAGTGGCTGACAAGACTATTTTGGCAACCGCAAGGAGGAACTCCTTCCACCAAGGCCCTAACTATGAAGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ENOD93

Protein Analysis

106

Amino Acids

11.19

Weight (kDa)

9.92

Isoelectric Point (pI)

33.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ENOD93 PF03386 22 - 96 4.5e-41 Early nodulin 93 ENOD93 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 64
AciI CCGC 2 cut(s) 224, 273
AfeI AGCGCT 1 cut(s) 74
AfiI CCNNNNNNNGG 2 cut(s) 64, 279
AjuI GAANNNNNNNTTGG 2 cut(s) 154, 186
AluBI AGCT 2 cut(s) 98, 113
AluI AGCT 2 cut(s) 98, 113
Alw26I GTCTC 1 cut(s) 21
AlwNI CAGNNNCTG 1 cut(s) 227
Aor51HI AGCGCT 1 cut(s) 74
AoxI GGCC 1 cut(s) 298
ApeKI GCWGC 2 cut(s) 110, 166
ArsI GACNNNNNNTTYG 1 cut(s) 31
Asp700I GAANNNNTTC 1 cut(s) 287
AspLEI GCGC 1 cut(s) 75
AspS9I GGNCC 1 cut(s) 299
BbvI GCAGC 2 cut(s) 122, 153
BcoDI GTCTC 1 cut(s) 21
BfaI CTAG 1 cut(s) 153
BfoI RGCGCY 1 cut(s) 76
BisI GCNGC 3 cut(s) 111, 167, 225
BlpI GCTNAGC 1 cut(s) 69
BlsI GCNGC 3 cut(s) 112, 168, 226
BmgT120I GGNCC 1 cut(s) 299
BpmI CTGGAG 1 cut(s) 120
Bpu10I CCTNAGC 1 cut(s) 201
Bpu1102I GCTNAGC 1 cut(s) 69
BsaJI CCNNGG 3 cut(s) 27, 170, 295
Bsc4I CCNNNNNNNGG 2 cut(s) 64, 279
Bse1I ACTGG 1 cut(s) 132
Bse3DI GCAATG 1 cut(s) 126
BseDI CCNNGG 3 cut(s) 27, 170, 295
BseLI CCNNNNNNNGG 2 cut(s) 64, 279
BseMI GCAATG 1 cut(s) 126
BseMII CTCAG 1 cut(s) 215
BseNI ACTGG 1 cut(s) 132
BseXI GCAGC 2 cut(s) 122, 153
BshFI GGCC 1 cut(s) 300
BslI CCNNNNNNNGG 2 cut(s) 64, 279
BsmAI GTCTC 1 cut(s) 21
BsmI GAATGC 1 cut(s) 168
BsnI GGCC 1 cut(s) 300
Bsp1720I GCTNAGC 1 cut(s) 69
Bsp19I CCATGG 1 cut(s) 27
BspACI CCGC 2 cut(s) 224, 273
BspANI GGCC 1 cut(s) 300
BspCNI CTCAG 1 cut(s) 214
BspHI TCATGA 1 cut(s) 82
BsrDI GCAATG 1 cut(s) 126
BsrI ACTGG 1 cut(s) 132
BssECI CCNNGG 3 cut(s) 27, 170, 295
BssT1I CCWWGG 3 cut(s) 27, 170, 295
BstDEI CTNAG 2 cut(s) 69, 201
BstDSI CCRYGG 1 cut(s) 27
BstH2I RGCGCY 1 cut(s) 76
BstHHI GCGC 1 cut(s) 75
BstMAI GTCTC 1 cut(s) 21
BstMWI GCNNNNNNNGC 3 cut(s) 119, 128, 233
BstV1I GCAGC 2 cut(s) 122, 153
BsuRI GGCC 1 cut(s) 300
BtgI CCRYGG 1 cut(s) 27
BtsI GCAGTG 1 cut(s) 195
BtsIMutI CAGTG 2 cut(s) 139, 195
CaiI CAGNNNCTG 1 cut(s) 227
CciI TCATGA 1 cut(s) 82
CfoI GCGC 1 cut(s) 75
Cfr13I GGNCC 1 cut(s) 299
CviAII CATG 3 cut(s) 28, 83, 315
CviJI RGCY 9 cut(s) 42, 68, 98, 113, 182, 206, 227, 251, 300
CviKI_1 RGCY 9 cut(s) 42, 68, 98, 113, 182, 206, 227, 251, 300
DdeI CTNAG 2 cut(s) 69, 201
Eco130I CCWWGG 3 cut(s) 27, 170, 295
Eco47III AGCGCT 1 cut(s) 74
EcoO109I RGGNCCY 1 cut(s) 299
EcoT14I CCWWGG 3 cut(s) 27, 170, 295
ErhI CCWWGG 3 cut(s) 27, 170, 295
FaeI CATG 3 cut(s) 31, 86, 318
FaiI YATR 7 cut(s) 29, 84, 212, 238, 245, 309, 316
FatI CATG 3 cut(s) 27, 82, 314
Fnu4HI GCNGC 3 cut(s) 111, 167, 225
Fsp4HI GCNGC 3 cut(s) 111, 167, 225
FspBI CTAG 1 cut(s) 153
GlaI GCGC 1 cut(s) 74
GluI GCNGC 3 cut(s) 111, 167, 225
GsuI CTGGAG 1 cut(s) 120
HaeII RGCGCY 1 cut(s) 76
HaeIII GGCC 1 cut(s) 300
HhaI GCGC 1 cut(s) 75
Hin1II CATG 3 cut(s) 31, 86, 318
Hin6I GCGC 1 cut(s) 73
HinP1I GCGC 1 cut(s) 73
HinfI GANTC 1 cut(s) 190
Hpy188I TCNGA 1 cut(s) 189
Hpy188III TCNNGA 1 cut(s) 83
HpyAV CCTTC 1 cut(s) 298
HpyCH4V TGCA 1 cut(s) 137
HpyF10VI GCNNNNNNNGC 3 cut(s) 119, 128, 233
HpyF3I CTNAG 2 cut(s) 69, 201
Hsp92II CATG 3 cut(s) 31, 86, 318
HspAI GCGC 1 cut(s) 73
LpnPI CCDG 6 cut(s) 56, 71, 84, 145, 188, 213
Lsp1109I GCAGC 2 cut(s) 122, 153
MaeI CTAG 1 cut(s) 153
MluCI AATT 1 cut(s) 138
MmeI TCCRAC 1 cut(s) 167
MnlI CCTC 2 cut(s) 79, 273
MroXI GAANNNNTTC 1 cut(s) 287
MseI TTAA 1 cut(s) 105
MslI CAYNNNNRTG 1 cut(s) 87
MspA1I CMGCKG 2 cut(s) 113, 224
Mva1269I GAATGC 1 cut(s) 168
MwoI GCNNNNNNNGC 3 cut(s) 119, 128, 233
NcoI CCATGG 1 cut(s) 27
NlaIII CATG 3 cut(s) 31, 86, 318
PagI TCATGA 1 cut(s) 82
PctI GAATGC 1 cut(s) 168
PdmI GAANNNNTTC 1 cut(s) 287
PfeI GAWTC 1 cut(s) 190
PflMI CCANNNNNTGG 1 cut(s) 64
PkrI GCNGC 3 cut(s) 112, 168, 226
PspPI GGNCC 1 cut(s) 299
PstNI CAGNNNCTG 1 cut(s) 227
PvuII CAGCTG 1 cut(s) 113
RseI CAYNNNNRTG 1 cut(s) 87
SaqAI TTAA 1 cut(s) 105
SatI GCNGC 3 cut(s) 111, 167, 225
Sau96I GGNCC 1 cut(s) 299
SetI ASST 2 cut(s) 100, 115
SmiMI CAYNNNNRTG 1 cut(s) 87
Sse9I AATT 1 cut(s) 138
SsiI CCGC 2 cut(s) 224, 273
SspMI CTAG 1 cut(s) 153
StyI CCWWGG 3 cut(s) 27, 170, 295
TasI AATT 1 cut(s) 138
TauI GCSGC 1 cut(s) 227
TfiI GAWTC 1 cut(s) 190
Tru1I TTAA 1 cut(s) 105
Tru9I TTAA 1 cut(s) 105
TscAI CASTG 2 cut(s) 139, 202
TseI GCWGC 2 cut(s) 110, 166
TspDTI ATGAA 2 cut(s) 39, 232
TspRI CASTG 2 cut(s) 139, 202
Van91I CCANNNNNTGG 1 cut(s) 64
XmnI GAANNNNTTC 1 cut(s) 287
XspI CTAG 1 cut(s) 153
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.