FvH4_2g35210

Early nodulin-93-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0003255
Physical Location & Seq
Forward (+)
25997477 .. 25999244
1768 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g35210.t1

Sequence Viewer

Length: 360 bp
ATGGGAATTCCGTCTGAGATGAGAGACATATGGGTTAACAACCGCAAAAACGCTCTGCTGATTCAGTCTCCATTTGAAGACCAGATTCAACAGAAAGCCCTCAGGGCCAAAACTTGCACCCAAGAGGGCGCTCGTAAGGGATTCAAGGCAGCTTGCATTTGGGGTGCTGTCAGCGCTGTGCCTACATTGACTGCTGTTCGTATGATTCCTTGGGCAAAGGCTAATCTCAATTATACTGCTCAAGCACTGATAATATCTGCTGCATCCATCGCCGCGTACTTTATCACAGCTGATAAAACTATATTGGAGTGTGCTAGAAAGAATGCACAGTTGCAACAAGCCTTGAGCCGCCAGCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ENOD93

Protein Analysis

120

Amino Acids

13.12

Weight (kDa)

9.88

Isoelectric Point (pI)

40.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ENOD93 PF03386 33 - 108 3.1e-38 Early nodulin 93 ENOD93 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 275
AciI CCGC 3 cut(s) 43, 273, 349
AcsI RAATTY 1 cut(s) 6
AfaI GTAC 1 cut(s) 278
AfeI AGCGCT 1 cut(s) 175
AgsI TTSAA 3 cut(s) 77, 89, 145
AluBI AGCT 2 cut(s) 152, 290
AluI AGCT 2 cut(s) 152, 290
Alw26I GTCTC 2 cut(s) 18, 72
Aor51HI AGCGCT 1 cut(s) 175
AoxI GGCC 1 cut(s) 105
ApeKI GCWGC 2 cut(s) 149, 260
ApoI RAATTY 1 cut(s) 6
AspLEI GCGC 2 cut(s) 131, 176
AspS9I GGNCC 1 cut(s) 105
AxyI CCTNAGG 1 cut(s) 101
BbsI GAAGAC 1 cut(s) 84
BbvI GCAGC 2 cut(s) 161, 247
BccI CCATC 1 cut(s) 275
BcoDI GTCTC 2 cut(s) 18, 72
BfaI CTAG 1 cut(s) 315
BfoI RGCGCY 2 cut(s) 132, 177
BglI GCCNNNNNGGC 1 cut(s) 104
BisI GCNGC 4 cut(s) 150, 261, 273, 349
BlsI GCNGC 4 cut(s) 151, 262, 274, 350
BmgT120I GGNCC 1 cut(s) 105
BmsI GCATC 1 cut(s) 272
BpiI GAAGAC 1 cut(s) 84
BpuEI CTTGAG 1 cut(s) 225
BsaJI CCNNGG 1 cut(s) 209
Bse21I CCTNAGG 1 cut(s) 101
BseDI CCNNGG 1 cut(s) 209
BseGI GGATG 1 cut(s) 263
BseMII CTCAG 2 cut(s) 6, 115
BseXI GCAGC 2 cut(s) 161, 247
Bsh1236I CGCG 1 cut(s) 275
BshFI GGCC 1 cut(s) 107
BsmAI GTCTC 2 cut(s) 18, 72
BsmI GAATGC 1 cut(s) 328
BsnI GGCC 1 cut(s) 107
BspACI CCGC 3 cut(s) 43, 273, 349
BspANI GGCC 1 cut(s) 107
BspCNI CTCAG 2 cut(s) 7, 114
BspFNI CGCG 1 cut(s) 275
BssECI CCNNGG 1 cut(s) 209
BssT1I CCWWGG 1 cut(s) 209
Bst4CI ACNGT 1 cut(s) 330
BstC8I GCNNGC 2 cut(s) 154, 353
BstDEI CTNAG 2 cut(s) 15, 101
BstF5I GGATG 1 cut(s) 263
BstFNI CGCG 1 cut(s) 275
BstH2I RGCGCY 2 cut(s) 132, 177
BstHHI GCGC 2 cut(s) 131, 176
BstMAI GTCTC 2 cut(s) 18, 72
BstMWI GCNNNNNNNGC 3 cut(s) 104, 173, 269
BstUI CGCG 1 cut(s) 275
BstV1I GCAGC 2 cut(s) 161, 247
BstV2I GAAGAC 1 cut(s) 84
Bsu36I CCTNAGG 1 cut(s) 101
BsuRI GGCC 1 cut(s) 107
BtgZI GCGATG 1 cut(s) 253
BtsCI GGATG 1 cut(s) 263
BtsIMutI CAGTG 1 cut(s) 245
Cac8I GCNNGC 2 cut(s) 154, 353
CfoI GCGC 2 cut(s) 131, 176
Cfr13I GGNCC 1 cut(s) 105
Csp6I GTAC 1 cut(s) 277
CviJI RGCY 7 cut(s) 98, 107, 152, 221, 290, 341, 348
CviKI_1 RGCY 7 cut(s) 98, 107, 152, 221, 290, 341, 348
CviQI GTAC 1 cut(s) 277
DdeI CTNAG 2 cut(s) 15, 101
Eco130I CCWWGG 1 cut(s) 209
Eco47III AGCGCT 1 cut(s) 175
Eco81I CCTNAGG 1 cut(s) 101
EcoRI GAATTC 1 cut(s) 6
EcoT14I CCWWGG 1 cut(s) 209
ErhI CCWWGG 1 cut(s) 209
FaiI YATR 5 cut(s) 29, 31, 203, 234, 302
FauNDI CATATG 1 cut(s) 29
Fnu4HI GCNGC 4 cut(s) 150, 261, 273, 349
FokI GGATG 1 cut(s) 250
Fsp4HI GCNGC 4 cut(s) 150, 261, 273, 349
FspBI CTAG 1 cut(s) 315
GlaI GCGC 2 cut(s) 130, 175
GluI GCNGC 4 cut(s) 150, 261, 273, 349
HaeII RGCGCY 2 cut(s) 132, 177
HaeIII GGCC 1 cut(s) 107
HhaI GCGC 2 cut(s) 131, 176
Hin6I GCGC 2 cut(s) 129, 174
HinP1I GCGC 2 cut(s) 129, 174
HincII GTYRAC 1 cut(s) 37
HindII GTYRAC 1 cut(s) 37
HinfI GANTC 4 cut(s) 61, 85, 141, 205
HpaI GTTAAC 1 cut(s) 37
Hpy166II GTNNAC 1 cut(s) 37
Hpy188I TCNGA 1 cut(s) 16
Hpy8I GTNNAC 1 cut(s) 37
HpyCH4III ACNGT 1 cut(s) 330
HpyCH4V TGCA 5 cut(s) 117, 156, 263, 326, 334
HpyF10VI GCNNNNNNNGC 3 cut(s) 104, 173, 269
HpyF3I CTNAG 2 cut(s) 15, 101
HspAI GCGC 2 cut(s) 129, 174
KspAI GTTAAC 1 cut(s) 37
LpnPI CCDG 2 cut(s) 88, 95
Lsp1109I GCAGC 2 cut(s) 161, 247
LweI GCATC 1 cut(s) 272
MaeI CTAG 1 cut(s) 315
MboII GAAGA 1 cut(s) 89
MluCI AATT 2 cut(s) 6, 229
MnlI CCTC 2 cut(s) 110, 118
MseI TTAA 1 cut(s) 36
MspA1I CMGCKG 1 cut(s) 290
Mva1269I GAATGC 1 cut(s) 328
MvnI CGCG 1 cut(s) 275
MwoI GCNNNNNNNGC 3 cut(s) 104, 173, 269
NdeI CATATG 1 cut(s) 29
PctI GAATGC 1 cut(s) 328
PfeI GAWTC 4 cut(s) 61, 85, 141, 205
PkrI GCNGC 4 cut(s) 151, 262, 274, 350
PspPI GGNCC 1 cut(s) 105
PvuII CAGCTG 1 cut(s) 290
RsaI GTAC 1 cut(s) 278
RsaNI GTAC 1 cut(s) 277
SaqAI TTAA 1 cut(s) 36
SatI GCNGC 4 cut(s) 150, 261, 273, 349
Sau96I GGNCC 1 cut(s) 105
SetI ASST 2 cut(s) 154, 292
SfaNI GCATC 1 cut(s) 272
SmlI CTYRAG 2 cut(s) 240, 343
SmoI CTYRAG 2 cut(s) 240, 343
Sse9I AATT 2 cut(s) 6, 229
SsiI CCGC 3 cut(s) 43, 273, 349
SspMI CTAG 1 cut(s) 315
StyI CCWWGG 1 cut(s) 209
TaaI ACNGT 1 cut(s) 330
TasI AATT 2 cut(s) 6, 229
TauI GCSGC 2 cut(s) 275, 351
TfiI GAWTC 4 cut(s) 61, 85, 141, 205
Tru1I TTAA 1 cut(s) 36
Tru9I TTAA 1 cut(s) 36
TscAI CASTG 1 cut(s) 252
TseI GCWGC 2 cut(s) 149, 260
TspRI CASTG 1 cut(s) 252
XapI RAATTY 1 cut(s) 6
XspI CTAG 1 cut(s) 315
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.