MD15G1176400.v1.1

Early nodulin-93-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0003255
Physical Location & Seq
Reverse (-)
13785374 .. 13788640
3267 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1176400.v1.1.491

Sequence Viewer

Length: 324 bp
ATGTCAAAAAATATGGCTCAGTCTCCCTTTGAGAAGACCTCAATTGACCAACTGGCCATGGCTAAGCGCTATTCCCGTGAGGGTGTAAAAGCAGGAGCTAAGGCAGCTGTTGTTGCCACCATTGCCACTGCCATTCCAACTATGGCTAGTGTGAGGATGCTACCTTGGGCAAGAGCCAATCTCAATCCCACTGCTCAGGCCCTCATAATCTCCACAGTGGCTGGAATGGCATATTTCATCGTGGCTGACAAGACTATTTTGGCAACTGCAAGAAGGAACTCATTCAATCAAGTGGCTCACCATGGAGCAAGAATTAATCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ENOD93

Protein Analysis

108

Amino Acids

11.48

Weight (kDa)

11.03

Isoelectric Point (pI)

33.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ENOD93 PF03386 17 - 94 4.7e-39 Early nodulin 93 ENOD93 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcoI YGGCCR 1 cut(s) 54
AfeI AGCGCT 1 cut(s) 68
AgsI TTSAA 1 cut(s) 286
AluBI AGCT 2 cut(s) 98, 107
AluI AGCT 2 cut(s) 98, 107
Alw26I GTCTC 1 cut(s) 27
AlwNI CAGNNNCTG 1 cut(s) 221
Aor51HI AGCGCT 1 cut(s) 68
AoxI GGCC 2 cut(s) 54, 198
ApeKI GCWGC 1 cut(s) 104
AseI ATTAAT 1 cut(s) 315
Asp700I GAANNNNTTC 1 cut(s) 281
AspLEI GCGC 1 cut(s) 69
AspS9I GGNCC 1 cut(s) 199
AsuHPI GGTGA 1 cut(s) 290
BalI TGGCCA 1 cut(s) 56
BbsI GAAGAC 1 cut(s) 41
BbvI GCAGC 1 cut(s) 116
BcoDI GTCTC 1 cut(s) 27
BfaI CTAG 1 cut(s) 147
BfoI RGCGCY 1 cut(s) 70
BisI GCNGC 1 cut(s) 105
BlpI GCTNAGC 1 cut(s) 63
BlsI GCNGC 1 cut(s) 106
BmgT120I GGNCC 1 cut(s) 199
BmsI GCATC 1 cut(s) 147
BpiI GAAGAC 1 cut(s) 41
BplI GAGNNNNNCTC 4 cut(s) 23, 55, 165, 197
Bpu10I CCTNAGC 2 cut(s) 99, 195
Bpu1102I GCTNAGC 1 cut(s) 63
BsaJI CCNNGG 3 cut(s) 57, 164, 301
Bse1I ACTGG 1 cut(s) 57
Bse3DI GCAATG 1 cut(s) 120
BseDI CCNNGG 3 cut(s) 57, 164, 301
BseGI GGATG 1 cut(s) 162
BseMI GCAATG 1 cut(s) 120
BseMII CTCAG 2 cut(s) 32, 209
BseNI ACTGG 1 cut(s) 57
BseXI GCAGC 1 cut(s) 116
BshFI GGCC 2 cut(s) 56, 200
BsmAI GTCTC 1 cut(s) 27
BsnI GGCC 2 cut(s) 56, 200
Bsp1720I GCTNAGC 1 cut(s) 63
Bsp19I CCATGG 2 cut(s) 57, 301
BspANI GGCC 2 cut(s) 56, 200
BspCNI CTCAG 2 cut(s) 31, 208
BsrDI GCAATG 1 cut(s) 120
BsrI ACTGG 1 cut(s) 57
BssECI CCNNGG 3 cut(s) 57, 164, 301
BssT1I CCWWGG 3 cut(s) 57, 164, 301
Bst4CI ACNGT 1 cut(s) 217
BstDEI CTNAG 4 cut(s) 18, 63, 99, 195
BstDSI CCRYGG 2 cut(s) 57, 301
BstF5I GGATG 1 cut(s) 162
BstH2I RGCGCY 1 cut(s) 70
BstHHI GCGC 1 cut(s) 69
BstMAI GTCTC 1 cut(s) 27
BstMWI GCNNNNNNNGC 4 cut(s) 104, 113, 122, 227
BstV1I GCAGC 1 cut(s) 116
BstV2I GAAGAC 1 cut(s) 41
BsuRI GGCC 2 cut(s) 56, 200
BtgI CCRYGG 2 cut(s) 57, 301
BtsCI GGATG 1 cut(s) 162
BtsI GCAGTG 2 cut(s) 126, 189
BtsIMutI CAGTG 3 cut(s) 126, 189, 222
CaiI CAGNNNCTG 1 cut(s) 221
CfoI GCGC 1 cut(s) 69
Cfr13I GGNCC 1 cut(s) 199
CviAII CATG 2 cut(s) 58, 302
DdeI CTNAG 4 cut(s) 18, 63, 99, 195
EaeI YGGCCR 1 cut(s) 54
Eco130I CCWWGG 3 cut(s) 57, 164, 301
Eco47III AGCGCT 1 cut(s) 68
EcoO109I RGGNCCY 1 cut(s) 199
EcoT14I CCWWGG 3 cut(s) 57, 164, 301
ErhI CCWWGG 3 cut(s) 57, 164, 301
FaeI CATG 2 cut(s) 61, 305
FaiI YATR 6 cut(s) 14, 59, 143, 206, 232, 303
FatI CATG 2 cut(s) 57, 301
Fnu4HI GCNGC 1 cut(s) 105
FokI GGATG 1 cut(s) 169
Fsp4HI GCNGC 1 cut(s) 105
FspBI CTAG 1 cut(s) 147
GlaI GCGC 1 cut(s) 68
GluI GCNGC 1 cut(s) 105
HaeII RGCGCY 1 cut(s) 70
HaeIII GGCC 2 cut(s) 56, 200
HhaI GCGC 1 cut(s) 69
Hin1II CATG 2 cut(s) 61, 305
Hin6I GCGC 1 cut(s) 67
HinP1I GCGC 1 cut(s) 67
HphI GGTGA 1 cut(s) 290
HpyAV CCTTC 1 cut(s) 267
HpyCH4III ACNGT 1 cut(s) 217
HpyCH4V TGCA 1 cut(s) 269
HpyF10VI GCNNNNNNNGC 4 cut(s) 104, 113, 122, 227
HpyF3I CTNAG 4 cut(s) 18, 63, 99, 195
Hsp92II CATG 2 cut(s) 61, 305
HspAI GCGC 1 cut(s) 67
LmnI GCTCC 2 cut(s) 95, 305
LpnPI CCDG 4 cut(s) 38, 78, 182, 207
Lsp1109I GCAGC 1 cut(s) 116
LweI GCATC 1 cut(s) 147
MaeI CTAG 1 cut(s) 147
MboII GAAGA 1 cut(s) 46
MfeI CAATTG 1 cut(s) 42
MlsI TGGCCA 1 cut(s) 56
MluCI AATT 2 cut(s) 42, 312
MluNI TGGCCA 1 cut(s) 56
MmeI TCCRAC 1 cut(s) 161
MnlI CCTC 4 cut(s) 49, 73, 147, 212
Mox20I TGGCCA 1 cut(s) 56
MroXI GAANNNNTTC 1 cut(s) 281
MscI TGGCCA 1 cut(s) 56
MseI TTAA 1 cut(s) 315
Msp20I TGGCCA 1 cut(s) 56
MspA1I CMGCKG 1 cut(s) 107
MunI CAATTG 1 cut(s) 42
MwoI GCNNNNNNNGC 4 cut(s) 104, 113, 122, 227
NcoI CCATGG 2 cut(s) 57, 301
NlaIII CATG 2 cut(s) 61, 305
PdmI GAANNNNTTC 1 cut(s) 281
PkrI GCNGC 1 cut(s) 106
PshBI ATTAAT 1 cut(s) 315
PspPI GGNCC 1 cut(s) 199
PstNI CAGNNNCTG 1 cut(s) 221
PvuII CAGCTG 1 cut(s) 107
SaqAI TTAA 1 cut(s) 315
SatI GCNGC 1 cut(s) 105
Sau96I GGNCC 1 cut(s) 199
SetI ASST 4 cut(s) 41, 100, 109, 166
SfaNI GCATC 1 cut(s) 147
Sse9I AATT 2 cut(s) 42, 312
SspMI CTAG 1 cut(s) 147
StyI CCWWGG 3 cut(s) 57, 164, 301
TaaI ACNGT 1 cut(s) 217
TasI AATT 2 cut(s) 42, 312
Tru1I TTAA 1 cut(s) 315
Tru9I TTAA 1 cut(s) 315
TscAI CASTG 3 cut(s) 133, 196, 222
TseI GCWGC 1 cut(s) 104
TspDTI ATGAA 1 cut(s) 226
TspRI CASTG 3 cut(s) 133, 196, 222
VspI ATTAAT 1 cut(s) 315
XcmI CCANNNNNNNNNTGG 1 cut(s) 139
XmnI GAANNNNTTC 1 cut(s) 281
XspI CTAG 1 cut(s) 147
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.