Rh6AG464300

Early nodulin-93-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0003255
Physical Location & Seq
Reverse (-)
64678229 .. 64679485
1257 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG464300.1

Sequence Viewer

Length: 360 bp
ATGGGAATTCCATCGGGAATGAGGGACATGTGGGTGAACAACCGTCGAAACGCTCTGCTGATTCCTTCGCCATATGAAGATGAGATTCAACACAAGGCCTTAAGGGCCAAAACTTGCACCCAAGAGGGCGCTCGTCAGGGATTCAAGGCAGCTTGCATTTGGGGTGCTGTCAGCGCTGTGCCCACATTGGCTGCTGTTCGTACGATTCCTTGGGCAAAGGCTAACCTCAATTATACTGCTCAAGCACTGATGATATCTGCTGCATCAATTGCTGCTTACTTCATCACCGCTGATAAAACTATCTTGGAGTGTGCTAGAAAAAATGCACAGTTGCAACAAGCCTTGAGCCGCCAGCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ENOD93

Protein Analysis

119

Amino Acids

13.05

Weight (kDa)

9.78

Isoelectric Point (pI)

44.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ENOD93 PF03386 33 - 108 6.7e-37 Early nodulin 93 ENOD93 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 288, 349
AcsI RAATTY 1 cut(s) 6
AfaI GTAC 1 cut(s) 202
AfeI AGCGCT 1 cut(s) 175
AflII CTTAAG 1 cut(s) 100
AflIII ACRYGT 1 cut(s) 27
AgsI TTSAA 2 cut(s) 89, 145
AluBI AGCT 1 cut(s) 152
AluI AGCT 1 cut(s) 152
Aor51HI AGCGCT 1 cut(s) 175
AoxI GGCC 2 cut(s) 96, 105
ApeKI GCWGC 4 cut(s) 149, 191, 260, 272
ApoI RAATTY 1 cut(s) 6
AspLEI GCGC 2 cut(s) 131, 176
AspS9I GGNCC 1 cut(s) 105
AsuHPI GGTGA 2 cut(s) 46, 277
BaeGI GKGCMC 1 cut(s) 183
BbvI GCAGC 4 cut(s) 161, 178, 247, 259
BccI CCATC 1 cut(s) 19
BfaI CTAG 1 cut(s) 315
BfoI RGCGCY 2 cut(s) 132, 177
BfrI CTTAAG 1 cut(s) 100
BglI GCCNNNNNGGC 1 cut(s) 104
BisI GCNGC 5 cut(s) 150, 192, 261, 273, 349
BlsI GCNGC 5 cut(s) 151, 193, 262, 274, 350
BmgT120I GGNCC 1 cut(s) 105
BmsI GCATC 1 cut(s) 272
BpuEI CTTGAG 1 cut(s) 225
BsaJI CCNNGG 1 cut(s) 209
BseDI CCNNGG 1 cut(s) 209
BseSI GKGCMC 1 cut(s) 183
BseXI GCAGC 4 cut(s) 161, 178, 247, 259
BshFI GGCC 2 cut(s) 98, 107
BsiWI CGTACG 1 cut(s) 200
BslFI GGGAC 1 cut(s) 38
BsmFI GGGAC 1 cut(s) 38
BsnI GGCC 2 cut(s) 98, 107
Bsp1286I GDGCHC 1 cut(s) 183
BspACI CCGC 2 cut(s) 288, 349
BspANI GGCC 2 cut(s) 98, 107
BspTI CTTAAG 1 cut(s) 100
BssECI CCNNGG 1 cut(s) 209
BssT1I CCWWGG 1 cut(s) 209
Bst4CI ACNGT 2 cut(s) 44, 330
BstAFI CTTAAG 1 cut(s) 100
BstAPI GCANNNNNTGC 1 cut(s) 269
BstC8I GCNNGC 2 cut(s) 154, 353
BstH2I RGCGCY 2 cut(s) 132, 177
BstHHI GCGC 2 cut(s) 131, 176
BstMWI GCNNNNNNNGC 3 cut(s) 104, 173, 269
BstNSI RCATGY 1 cut(s) 31
BstSLI GKGCMC 1 cut(s) 183
BstV1I GCAGC 4 cut(s) 161, 178, 247, 259
BsuRI GGCC 2 cut(s) 98, 107
BtsIMutI CAGTG 1 cut(s) 245
Cac8I GCNNGC 2 cut(s) 154, 353
CfoI GCGC 2 cut(s) 131, 176
Cfr13I GGNCC 1 cut(s) 105
Csp6I GTAC 1 cut(s) 201
CviAII CATG 1 cut(s) 28
CviJI RGCY 7 cut(s) 98, 107, 152, 191, 221, 341, 348
CviKI_1 RGCY 7 cut(s) 98, 107, 152, 191, 221, 341, 348
CviQI GTAC 1 cut(s) 201
Eco130I CCWWGG 1 cut(s) 209
Eco147I AGGCCT 1 cut(s) 98
Eco32I GATATC 1 cut(s) 255
Eco47III AGCGCT 1 cut(s) 175
EcoRI GAATTC 1 cut(s) 6
EcoRV GATATC 1 cut(s) 255
EcoT14I CCWWGG 1 cut(s) 209
ErhI CCWWGG 1 cut(s) 209
FaeI CATG 1 cut(s) 31
FaiI YATR 4 cut(s) 29, 73, 75, 234
FaqI GGGAC 1 cut(s) 38
FatI CATG 1 cut(s) 27
FauNDI CATATG 1 cut(s) 73
Fnu4HI GCNGC 5 cut(s) 150, 192, 261, 273, 349
Fsp4HI GCNGC 5 cut(s) 150, 192, 261, 273, 349
FspBI CTAG 1 cut(s) 315
GlaI GCGC 2 cut(s) 130, 175
GluI GCNGC 5 cut(s) 150, 192, 261, 273, 349
HaeII RGCGCY 2 cut(s) 132, 177
HaeIII GGCC 2 cut(s) 98, 107
HhaI GCGC 2 cut(s) 131, 176
Hin1II CATG 1 cut(s) 31
Hin6I GCGC 2 cut(s) 129, 174
HinP1I GCGC 2 cut(s) 129, 174
HinfI GANTC 4 cut(s) 61, 85, 141, 205
HphI GGTGA 2 cut(s) 46, 277
Hpy166II GTNNAC 1 cut(s) 37
Hpy188III TCNNGA 1 cut(s) 15
Hpy8I GTNNAC 1 cut(s) 37
Hpy99I CGWCG 1 cut(s) 48
HpyAV CCTTC 1 cut(s) 75
HpyCH4III ACNGT 2 cut(s) 44, 330
HpyCH4V TGCA 5 cut(s) 117, 156, 263, 326, 334
HpyF10VI GCNNNNNNNGC 3 cut(s) 104, 173, 269
Hsp92II CATG 1 cut(s) 31
HspAI GCGC 2 cut(s) 129, 174
LpnPI CCDG 1 cut(s) 122
Lsp1109I GCAGC 4 cut(s) 161, 178, 247, 259
LweI GCATC 1 cut(s) 272
MaeI CTAG 1 cut(s) 315
MboII GAAGA 1 cut(s) 89
MfeI CAATTG 1 cut(s) 267
MhlI GDGCHC 1 cut(s) 183
MluCI AATT 3 cut(s) 6, 229, 267
MnlI CCTC 3 cut(s) 15, 118, 236
MseI TTAA 1 cut(s) 101
MslI CAYNNNNRTG 1 cut(s) 32
MspA1I CMGCKG 1 cut(s) 290
MspCI CTTAAG 1 cut(s) 100
MunI CAATTG 1 cut(s) 267
MwoI GCNNNNNNNGC 3 cut(s) 104, 173, 269
NdeI CATATG 1 cut(s) 73
NlaIII CATG 1 cut(s) 31
NspI RCATGY 1 cut(s) 31
PceI AGGCCT 1 cut(s) 98
PciI ACATGT 1 cut(s) 27
PfeI GAWTC 4 cut(s) 61, 85, 141, 205
Pfl23II CGTACG 1 cut(s) 200
PkrI GCNGC 5 cut(s) 151, 193, 262, 274, 350
PscI ACATGT 1 cut(s) 27
PspLI CGTACG 1 cut(s) 200
PspPI GGNCC 1 cut(s) 105
RsaI GTAC 1 cut(s) 202
RsaNI GTAC 1 cut(s) 201
RseI CAYNNNNRTG 1 cut(s) 32
SaqAI TTAA 1 cut(s) 101
SatI GCNGC 5 cut(s) 150, 192, 261, 273, 349
Sau96I GGNCC 1 cut(s) 105
SduI GDGCHC 1 cut(s) 183
SetI ASST 2 cut(s) 154, 228
SfaNI GCATC 1 cut(s) 272
SfiI GGCCNNNNNGGCC 1 cut(s) 104
SmiMI CAYNNNNRTG 1 cut(s) 32
SmlI CTYRAG 3 cut(s) 100, 240, 343
SmoI CTYRAG 3 cut(s) 100, 240, 343
Sse9I AATT 3 cut(s) 6, 229, 267
SseBI AGGCCT 1 cut(s) 98
SsiI CCGC 2 cut(s) 288, 349
SspMI CTAG 1 cut(s) 315
StuI AGGCCT 1 cut(s) 98
StyI CCWWGG 1 cut(s) 209
TaaI ACNGT 2 cut(s) 44, 330
TaqI TCGA 1 cut(s) 46
TasI AATT 3 cut(s) 6, 229, 267
TauI GCSGC 1 cut(s) 351
TfiI GAWTC 4 cut(s) 61, 85, 141, 205
Tru1I TTAA 1 cut(s) 101
Tru9I TTAA 1 cut(s) 101
TscAI CASTG 1 cut(s) 252
TseI GCWGC 4 cut(s) 149, 191, 260, 272
TspDTI ATGAA 2 cut(s) 90, 271
TspRI CASTG 1 cut(s) 252
Vha464I CTTAAG 1 cut(s) 100
XapI RAATTY 1 cut(s) 6
XceI RCATGY 1 cut(s) 31
XspI CTAG 1 cut(s) 315
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.