MD08G1110000.v1.1

Early nodulin-93-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0003255
Physical Location & Seq
Forward (+)
9674055 .. 9676683
2629 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1110000.v1.1.491

Sequence Viewer

Length: 354 bp
ATGGGGATTCCATCGGAATTGAGGGACATGTGGGTGAACCGCAAGGATGCTCTGGTGATTCCTTCGCCAGCTGAAGAGGCGAAGCTGCTGAGATCCAAGAAGTGCATCGACGAGGGTGTTCAGGAGGGATTCAAGTACGGTGCTGTTGCGGCTGTTATCTCAAGTGTGGCTACGTTGGGTGCTGTTCGTACGGTACCTTGGGCAAAGTATAACCTCAACTACACCGCTCAAGCACTCATCATCAGTGCTGCAACAATTTCTACATACTTCATCACCGTTGATAAAACCATCTTGGCATGTGCAAGAAAAAATGCCCTGTTGCAAGACAGCTTGAGACGCCAGCGCGAGCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ENOD93

Protein Analysis

118

Amino Acids

12.9

Weight (kDa)

9.43

Isoelectric Point (pI)

45.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ENOD93 PF03386 28 - 104 5.2e-34 Early nodulin 93 ENOD93 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 193
AccB1I GGYRCC 1 cut(s) 193
AccBSI CCGCTC 1 cut(s) 227
AccII CGCG 1 cut(s) 345
AciI CCGC 3 cut(s) 40, 149, 225
AclWI GGATC 1 cut(s) 87
AcuI CTGAAG 1 cut(s) 93
AcyI GRCGYC 1 cut(s) 337
AfaI GTAC 3 cut(s) 137, 190, 195
AflIII ACRYGT 1 cut(s) 27
AgsI TTSAA 1 cut(s) 133
AluBI AGCT 3 cut(s) 71, 85, 330
AluI AGCT 3 cut(s) 71, 85, 330
Alw26I GTCTC 1 cut(s) 328
AlwI GGATC 1 cut(s) 87
ApeKI GCWGC 2 cut(s) 85, 248
Asp718I GGTACC 1 cut(s) 193
AspLEI GCGC 1 cut(s) 345
AsuHPI GGTGA 3 cut(s) 46, 67, 265
BanI GGYRCC 1 cut(s) 193
BbvI GCAGC 2 cut(s) 72, 235
BccI CCATC 2 cut(s) 19, 296
BcoDI GTCTC 1 cut(s) 328
BisI GCNGC 3 cut(s) 86, 150, 249
BlsI GCNGC 3 cut(s) 87, 151, 250
BmiI GGNNCC 1 cut(s) 195
BmsI GCATC 2 cut(s) 37, 114
BpuEI CTTGAG 3 cut(s) 145, 213, 352
BsaHI GRCGYC 1 cut(s) 337
BsaJI CCNNGG 1 cut(s) 197
BseDI CCNNGG 1 cut(s) 197
BseGI GGATG 1 cut(s) 52
BseMII CTCAG 1 cut(s) 80
BseXI GCAGC 2 cut(s) 72, 235
Bsh1236I CGCG 1 cut(s) 345
BshNI GGYRCC 1 cut(s) 193
BsiWI CGTACG 1 cut(s) 188
BslFI GGGAC 1 cut(s) 38
BsmAI GTCTC 1 cut(s) 328
BsmBI CGTCTC 1 cut(s) 328
BsmFI GGGAC 1 cut(s) 38
Bsp143I GATC 1 cut(s) 92
BspACI CCGC 3 cut(s) 40, 149, 225
BspCNI CTCAG 1 cut(s) 81
BspFNI CGCG 1 cut(s) 345
BspLI GGNNCC 1 cut(s) 195
BspPI GGATC 1 cut(s) 87
BspT107I GGYRCC 1 cut(s) 193
BsrBI CCGCTC 1 cut(s) 227
BssECI CCNNGG 1 cut(s) 197
BssMI GATC 1 cut(s) 92
BssNI GRCGYC 1 cut(s) 337
BssT1I CCWWGG 1 cut(s) 197
Bst4CI ACNGT 3 cut(s) 140, 193, 277
Bst6I CTCTTC 1 cut(s) 69
BstACI GRCGYC 1 cut(s) 337
BstC8I GCNNGC 3 cut(s) 69, 341, 347
BstDEI CTNAG 1 cut(s) 89
BstF5I GGATG 1 cut(s) 52
BstFNI CGCG 1 cut(s) 345
BstHHI GCGC 1 cut(s) 345
BstKTI GATC 1 cut(s) 95
BstMAI GTCTC 1 cut(s) 328
BstMBI GATC 1 cut(s) 92
BstMWI GCNNNNNNNGC 3 cut(s) 77, 149, 336
BstNSI RCATGY 2 cut(s) 31, 300
BstUI CGCG 1 cut(s) 345
BstV1I GCAGC 2 cut(s) 72, 235
BstX2I RGATCY 1 cut(s) 92
BstYI RGATCY 1 cut(s) 92
BtsCI GGATG 1 cut(s) 52
BtsIMutI CAGTG 1 cut(s) 250
Cac8I GCNNGC 3 cut(s) 69, 341, 347
CfoI GCGC 1 cut(s) 345
CseI GACGC 1 cut(s) 345
Csp6I GTAC 3 cut(s) 136, 189, 194
CviAII CATG 2 cut(s) 28, 297
CviJI RGCY 5 cut(s) 71, 85, 152, 170, 330
CviKI_1 RGCY 5 cut(s) 71, 85, 152, 170, 330
CviQI GTAC 3 cut(s) 136, 189, 194
DdeI CTNAG 1 cut(s) 89
DpnI GATC 1 cut(s) 94
DpnII GATC 1 cut(s) 92
Eam1104I CTCTTC 1 cut(s) 69
EarI CTCTTC 1 cut(s) 69
Eco130I CCWWGG 1 cut(s) 197
Eco57I CTGAAG 1 cut(s) 93
EcoT14I CCWWGG 1 cut(s) 197
ErhI CCWWGG 1 cut(s) 197
Esp3I CGTCTC 1 cut(s) 328
FaeI CATG 2 cut(s) 31, 300
FaiI YATR 4 cut(s) 29, 210, 265, 298
FaqI GGGAC 1 cut(s) 38
FatI CATG 2 cut(s) 27, 296
Fnu4HI GCNGC 3 cut(s) 86, 150, 249
FokI GGATG 1 cut(s) 59
Fsp4HI GCNGC 3 cut(s) 86, 150, 249
GlaI GCGC 1 cut(s) 344
GluI GCNGC 3 cut(s) 86, 150, 249
HgaI GACGC 1 cut(s) 345
HhaI GCGC 1 cut(s) 345
Hin1I GRCGYC 1 cut(s) 337
Hin1II CATG 2 cut(s) 31, 300
Hin6I GCGC 1 cut(s) 343
HinP1I GCGC 1 cut(s) 343
HinfI GANTC 3 cut(s) 7, 58, 129
HphI GGTGA 3 cut(s) 46, 67, 265
Hpy166II GTNNAC 1 cut(s) 37
Hpy188I TCNGA 1 cut(s) 16
Hpy188III TCNNGA 1 cut(s) 122
Hpy8I GTNNAC 1 cut(s) 37
Hpy99I CGWCG 1 cut(s) 113
HpyAV CCTTC 1 cut(s) 72
HpyCH4III ACNGT 3 cut(s) 140, 193, 277
HpyCH4IV ACGT 1 cut(s) 173
HpyCH4V TGCA 4 cut(s) 105, 251, 302, 322
HpyF10VI GCNNNNNNNGC 3 cut(s) 77, 149, 336
HpyF3I CTNAG 1 cut(s) 89
HpySE526I ACGT 1 cut(s) 173
Hsp92I GRCGYC 1 cut(s) 337
Hsp92II CATG 2 cut(s) 31, 300
HspAI GCGC 1 cut(s) 343
KpnI GGTACC 1 cut(s) 197
Kzo9I GATC 1 cut(s) 92
LpnPI CCDG 4 cut(s) 38, 81, 107, 329
Lsp1109I GCAGC 2 cut(s) 72, 235
LweI GCATC 2 cut(s) 37, 114
MaeII ACGT 1 cut(s) 173
MalI GATC 1 cut(s) 94
MbiI CCGCTC 1 cut(s) 227
MboI GATC 1 cut(s) 92
MboII GAAGA 1 cut(s) 86
MflI RGATCY 1 cut(s) 92
MluCI AATT 2 cut(s) 17, 255
MnlI CCTC 5 cut(s) 15, 70, 106, 118, 224
MslI CAYNNNNRTG 1 cut(s) 32
MspA1I CMGCKG 1 cut(s) 71
MvnI CGCG 1 cut(s) 345
MwoI GCNNNNNNNGC 3 cut(s) 77, 149, 336
NdeII GATC 1 cut(s) 92
NlaIII CATG 2 cut(s) 31, 300
NlaIV GGNNCC 1 cut(s) 195
NspI RCATGY 2 cut(s) 31, 300
PciI ACATGT 1 cut(s) 27
PfeI GAWTC 3 cut(s) 7, 58, 129
Pfl23II CGTACG 1 cut(s) 188
PkrI GCNGC 3 cut(s) 87, 151, 250
PscI ACATGT 1 cut(s) 27
PspLI CGTACG 1 cut(s) 188
PspN4I GGNNCC 1 cut(s) 195
PsuI RGATCY 1 cut(s) 92
PvuII CAGCTG 1 cut(s) 71
RsaI GTAC 3 cut(s) 137, 190, 195
RsaNI GTAC 3 cut(s) 136, 189, 194
RseI CAYNNNNRTG 1 cut(s) 32
SatI GCNGC 3 cut(s) 86, 150, 249
Sau3AI GATC 1 cut(s) 92
SetI ASST 6 cut(s) 73, 87, 176, 199, 216, 332
SfaNI GCATC 2 cut(s) 37, 114
SmiMI CAYNNNNRTG 1 cut(s) 32
SmlI CTYRAG 3 cut(s) 160, 228, 331
SmoI CTYRAG 3 cut(s) 160, 228, 331
Sse9I AATT 2 cut(s) 17, 255
SsiI CCGC 3 cut(s) 40, 149, 225
StyI CCWWGG 1 cut(s) 197
TaaI ACNGT 3 cut(s) 140, 193, 277
TaiI ACGT 1 cut(s) 176
TaqI TCGA 1 cut(s) 108
TasI AATT 2 cut(s) 17, 255
TauI GCSGC 1 cut(s) 152
TfiI GAWTC 3 cut(s) 7, 58, 129
TscAI CASTG 1 cut(s) 250
TseI GCWGC 2 cut(s) 85, 248
TspDTI ATGAA 1 cut(s) 259
TspRI CASTG 1 cut(s) 250
XceI RCATGY 2 cut(s) 31, 300
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.