pycom02g02970

Early nodulin-93-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0003255
Physical Location & Seq
Reverse (-)
1906033 .. 1907183
1151 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g02970.1

Sequence Viewer

Length: 312 bp
ATGGCAAGAAATCTGCCTCAGTCTCCCTATGAGAAGACCTCAATTGACCAGTTGGCCTTGGCTAAGCACTGCTCTCGCGAGGGTGTAAAAGCAGGAGCTAAGGCAGCTGTTGTTGCTACCATTGCCACAGCCATTCCAACTCTGGCTAGTGTGAGGATGCTGCCTTGGGCAAGAGCCAATCTCAACCCCACTGCTCAGGCCCTCATAATCTCTACAGTGGCTGGAATGGCATACTTCATCGTGGCTGACAAGACTGTTTTGGCAACGGCAAGAAGGAACTCATTCAATCAAGTGGCTCACCATGAAGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ENOD93

Protein Analysis

104

Amino Acids

10.94

Weight (kDa)

10.12

Isoelectric Point (pI)

27.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ENOD93 PF03386 17 - 94 6.6e-40 Early nodulin 93 ENOD93 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 78
AgsI TTSAA 1 cut(s) 286
AluBI AGCT 2 cut(s) 98, 107
AluI AGCT 2 cut(s) 98, 107
Alw26I GTCTC 1 cut(s) 27
AlwNI CAGNNNCTG 1 cut(s) 221
AoxI GGCC 2 cut(s) 54, 198
ApeKI GCWGC 2 cut(s) 104, 160
Asp700I GAANNNNTTC 1 cut(s) 281
AspS9I GGNCC 1 cut(s) 199
AsuHPI GGTGA 1 cut(s) 290
BbsI GAAGAC 1 cut(s) 41
BbvI GCAGC 2 cut(s) 116, 147
BceAI ACGGC 1 cut(s) 282
BcgI CGANNNNNNTGC 2 cut(s) 56, 90
BcoDI GTCTC 1 cut(s) 27
BfaI CTAG 1 cut(s) 147
BfmI CTRYAG 1 cut(s) 213
BisI GCNGC 2 cut(s) 105, 161
BlpI GCTNAGC 1 cut(s) 63
BlsI GCNGC 2 cut(s) 106, 162
BmgT120I GGNCC 1 cut(s) 199
BmsI GCATC 1 cut(s) 147
BpiI GAAGAC 1 cut(s) 41
BplI GAGNNNNNCTC 4 cut(s) 23, 55, 165, 197
Bpu10I CCTNAGC 2 cut(s) 99, 195
Bpu1102I GCTNAGC 1 cut(s) 63
BsaJI CCNNGG 2 cut(s) 57, 164
Bse1I ACTGG 1 cut(s) 49
Bse3DI GCAATG 1 cut(s) 120
BseDI CCNNGG 2 cut(s) 57, 164
BseGI GGATG 1 cut(s) 162
BseMI GCAATG 1 cut(s) 120
BseMII CTCAG 2 cut(s) 32, 209
BseNI ACTGG 1 cut(s) 49
BseXI GCAGC 2 cut(s) 116, 147
Bsh1236I CGCG 1 cut(s) 78
BshFI GGCC 2 cut(s) 56, 200
BsmAI GTCTC 1 cut(s) 27
BsnI GGCC 2 cut(s) 56, 200
Bsp1720I GCTNAGC 1 cut(s) 63
Bsp68I TCGCGA 1 cut(s) 78
BspANI GGCC 2 cut(s) 56, 200
BspCNI CTCAG 2 cut(s) 31, 208
BspFNI CGCG 1 cut(s) 78
BsrDI GCAATG 1 cut(s) 120
BsrI ACTGG 1 cut(s) 49
BssECI CCNNGG 2 cut(s) 57, 164
BssT1I CCWWGG 2 cut(s) 57, 164
Bst4CI ACNGT 2 cut(s) 217, 256
BstDEI CTNAG 4 cut(s) 18, 63, 99, 195
BstF5I GGATG 1 cut(s) 162
BstFNI CGCG 1 cut(s) 78
BstMAI GTCTC 1 cut(s) 27
BstMWI GCNNNNNNNGC 4 cut(s) 104, 113, 122, 227
BstSFI CTRYAG 1 cut(s) 213
BstUI CGCG 1 cut(s) 78
BstV1I GCAGC 2 cut(s) 116, 147
BstV2I GAAGAC 1 cut(s) 41
BsuRI GGCC 2 cut(s) 56, 200
BtsCI GGATG 1 cut(s) 162
BtsI GCAGTG 2 cut(s) 67, 189
BtsIMutI CAGTG 3 cut(s) 67, 189, 222
BtuMI TCGCGA 1 cut(s) 78
CaiI CAGNNNCTG 1 cut(s) 221
Cfr13I GGNCC 1 cut(s) 199
CviAII CATG 2 cut(s) 302, 309
DdeI CTNAG 4 cut(s) 18, 63, 99, 195
Eco130I CCWWGG 2 cut(s) 57, 164
EcoO109I RGGNCCY 1 cut(s) 199
EcoT14I CCWWGG 2 cut(s) 57, 164
ErhI CCWWGG 2 cut(s) 57, 164
FaeI CATG 2 cut(s) 305, 312
FaiI YATR 5 cut(s) 30, 206, 232, 303, 310
FatI CATG 2 cut(s) 301, 308
Fnu4HI GCNGC 2 cut(s) 105, 161
FokI GGATG 1 cut(s) 169
Fsp4HI GCNGC 2 cut(s) 105, 161
FspBI CTAG 1 cut(s) 147
GluI GCNGC 2 cut(s) 105, 161
HaeIII GGCC 2 cut(s) 56, 200
Hin1II CATG 2 cut(s) 305, 312
HphI GGTGA 1 cut(s) 290
Hpy188III TCNNGA 1 cut(s) 77
HpyAV CCTTC 1 cut(s) 267
HpyCH4III ACNGT 2 cut(s) 217, 256
HpyF10VI GCNNNNNNNGC 4 cut(s) 104, 113, 122, 227
HpyF3I CTNAG 4 cut(s) 18, 63, 99, 195
Hsp92II CATG 2 cut(s) 305, 312
LmnI GCTCC 1 cut(s) 95
LpnPI CCDG 5 cut(s) 62, 78, 128, 182, 207
Lsp1109I GCAGC 2 cut(s) 116, 147
LweI GCATC 1 cut(s) 147
MaeI CTAG 1 cut(s) 147
MboII GAAGA 1 cut(s) 46
MfeI CAATTG 1 cut(s) 42
MluCI AATT 1 cut(s) 42
MmeI TCCRAC 1 cut(s) 161
MnlI CCTC 5 cut(s) 27, 49, 73, 147, 212
MroXI GAANNNNTTC 1 cut(s) 281
MspA1I CMGCKG 1 cut(s) 107
MunI CAATTG 1 cut(s) 42
MvnI CGCG 1 cut(s) 78
MwoI GCNNNNNNNGC 4 cut(s) 104, 113, 122, 227
NlaIII CATG 2 cut(s) 305, 312
NruI TCGCGA 1 cut(s) 78
PdmI GAANNNNTTC 1 cut(s) 281
PkrI GCNGC 2 cut(s) 106, 162
PspPI GGNCC 1 cut(s) 199
PstNI CAGNNNCTG 1 cut(s) 221
PvuII CAGCTG 1 cut(s) 107
RruI TCGCGA 1 cut(s) 78
SatI GCNGC 2 cut(s) 105, 161
Sau96I GGNCC 1 cut(s) 199
SetI ASST 3 cut(s) 41, 100, 109
SfaNI GCATC 1 cut(s) 147
SfcI CTRYAG 1 cut(s) 213
Sse9I AATT 1 cut(s) 42
SspMI CTAG 1 cut(s) 147
StyI CCWWGG 2 cut(s) 57, 164
TaaI ACNGT 2 cut(s) 217, 256
TasI AATT 1 cut(s) 42
TscAI CASTG 3 cut(s) 74, 196, 222
TseI GCWGC 2 cut(s) 104, 160
TspDTI ATGAA 1 cut(s) 226
TspRI CASTG 3 cut(s) 74, 196, 222
XcmI CCANNNNNNNNNTGG 1 cut(s) 139
XmnI GAANNNNTTC 1 cut(s) 281
XspI CTAG 1 cut(s) 147
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.