MD02G1036200.v1.1

Early nodulin-93-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0003255
Physical Location & Seq
Reverse (-)
2741241 .. 2742700
1460 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1036200.v1.1.491

Sequence Viewer

Length: 312 bp
ATGGCAAAAAGCATGGCTCAGTCTCCCTTTGAGAATACCTCAATTGACAAACTAGCCATGGCTAAGCGTTGTTCTCATGAGGGTGCAAAAGCAGGAGCTAAGGCAGCTGTTGTTGCCACCATTGCCACTGCTATTCCAACTCTGGCTAGTGTGAGGATGCTACCTTGGGCAAGAGCGAATCTCAATCCCGCTGCTCAGGCCCTCATAATCTCCACAGTGGCTGGAATGGCATATTTCATCGTGGCTGACAAGACTATTTTGGCAACTGCAAGAAGGAACTCGTTCAATCAAGTGGCTCGCCATGAAGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ENOD93

Protein Analysis

104

Amino Acids

10.84

Weight (kDa)

10.37

Isoelectric Point (pI)

32.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ENOD93 PF03386 17 - 94 3.2e-40 Early nodulin 93 ENOD93 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 189
AgsI TTSAA 1 cut(s) 286
AluBI AGCT 2 cut(s) 98, 107
AluI AGCT 2 cut(s) 98, 107
Alw26I GTCTC 1 cut(s) 27
AlwNI CAGNNNCTG 1 cut(s) 221
AoxI GGCC 1 cut(s) 198
ApeKI GCWGC 2 cut(s) 104, 191
Asp700I GAANNNNTTC 1 cut(s) 281
AspS9I GGNCC 1 cut(s) 199
BbvI GCAGC 2 cut(s) 116, 178
BcoDI GTCTC 1 cut(s) 27
BfaI CTAG 2 cut(s) 53, 147
BisI GCNGC 2 cut(s) 105, 192
BlpI GCTNAGC 1 cut(s) 63
BlsI GCNGC 2 cut(s) 106, 193
BmgT120I GGNCC 1 cut(s) 199
BmsI GCATC 1 cut(s) 147
BplI GAGNNNNNCTC 4 cut(s) 23, 55, 165, 197
Bpu10I CCTNAGC 2 cut(s) 99, 195
Bpu1102I GCTNAGC 1 cut(s) 63
BsaJI CCNNGG 2 cut(s) 57, 164
Bse3DI GCAATG 1 cut(s) 120
BseDI CCNNGG 2 cut(s) 57, 164
BseGI GGATG 1 cut(s) 162
BseMI GCAATG 1 cut(s) 120
BseMII CTCAG 2 cut(s) 32, 209
BseXI GCAGC 2 cut(s) 116, 178
BshFI GGCC 1 cut(s) 200
BsmAI GTCTC 1 cut(s) 27
BsnI GGCC 1 cut(s) 200
Bsp1720I GCTNAGC 1 cut(s) 63
Bsp19I CCATGG 1 cut(s) 57
BspACI CCGC 1 cut(s) 189
BspANI GGCC 1 cut(s) 200
BspCNI CTCAG 2 cut(s) 31, 208
BspHI TCATGA 1 cut(s) 76
BsrDI GCAATG 1 cut(s) 120
BssECI CCNNGG 2 cut(s) 57, 164
BssT1I CCWWGG 2 cut(s) 57, 164
Bst4CI ACNGT 1 cut(s) 217
BstC8I GCNNGC 1 cut(s) 298
BstDEI CTNAG 4 cut(s) 18, 63, 99, 195
BstDSI CCRYGG 1 cut(s) 57
BstF5I GGATG 1 cut(s) 162
BstMAI GTCTC 1 cut(s) 27
BstMWI GCNNNNNNNGC 5 cut(s) 104, 113, 122, 197, 227
BstV1I GCAGC 2 cut(s) 116, 178
BsuRI GGCC 1 cut(s) 200
BtgI CCRYGG 1 cut(s) 57
BtsCI GGATG 1 cut(s) 162
BtsI GCAGTG 1 cut(s) 126
BtsIMutI CAGTG 2 cut(s) 126, 222
Cac8I GCNNGC 1 cut(s) 298
CaiI CAGNNNCTG 1 cut(s) 221
CciI TCATGA 1 cut(s) 76
Cfr13I GGNCC 1 cut(s) 199
CviAII CATG 5 cut(s) 13, 58, 77, 302, 309
DdeI CTNAG 4 cut(s) 18, 63, 99, 195
Eco130I CCWWGG 2 cut(s) 57, 164
EcoO109I RGGNCCY 1 cut(s) 199
EcoT14I CCWWGG 2 cut(s) 57, 164
ErhI CCWWGG 2 cut(s) 57, 164
FaeI CATG 5 cut(s) 16, 61, 80, 305, 312
FaiI YATR 7 cut(s) 14, 59, 78, 206, 232, 303, 310
FatI CATG 5 cut(s) 12, 57, 76, 301, 308
FauI CCCGC 1 cut(s) 196
Fnu4HI GCNGC 2 cut(s) 105, 192
FokI GGATG 1 cut(s) 169
Fsp4HI GCNGC 2 cut(s) 105, 192
FspBI CTAG 2 cut(s) 53, 147
GluI GCNGC 2 cut(s) 105, 192
HaeIII GGCC 1 cut(s) 200
Hin1II CATG 5 cut(s) 16, 61, 80, 305, 312
HinfI GANTC 1 cut(s) 178
Hpy188III TCNNGA 1 cut(s) 77
HpyAV CCTTC 1 cut(s) 267
HpyCH4III ACNGT 1 cut(s) 217
HpyCH4V TGCA 2 cut(s) 86, 269
HpyF10VI GCNNNNNNNGC 5 cut(s) 104, 113, 122, 197, 227
HpyF3I CTNAG 4 cut(s) 18, 63, 99, 195
Hsp92II CATG 5 cut(s) 16, 61, 80, 305, 312
LmnI GCTCC 1 cut(s) 95
LpnPI CCDG 4 cut(s) 78, 128, 182, 207
Lsp1109I GCAGC 2 cut(s) 116, 178
LweI GCATC 1 cut(s) 147
MaeI CTAG 2 cut(s) 53, 147
MfeI CAATTG 1 cut(s) 42
MluCI AATT 1 cut(s) 42
MmeI TCCRAC 1 cut(s) 161
MnlI CCTC 4 cut(s) 49, 73, 147, 212
MroXI GAANNNNTTC 1 cut(s) 281
MslI CAYNNNNRTG 1 cut(s) 81
MspA1I CMGCKG 2 cut(s) 107, 191
MunI CAATTG 1 cut(s) 42
MwoI GCNNNNNNNGC 5 cut(s) 104, 113, 122, 197, 227
NcoI CCATGG 1 cut(s) 57
NlaIII CATG 5 cut(s) 16, 61, 80, 305, 312
PagI TCATGA 1 cut(s) 76
PdmI GAANNNNTTC 1 cut(s) 281
PfeI GAWTC 1 cut(s) 178
PkrI GCNGC 2 cut(s) 106, 193
PspPI GGNCC 1 cut(s) 199
PstNI CAGNNNCTG 1 cut(s) 221
PvuII CAGCTG 1 cut(s) 107
RseI CAYNNNNRTG 1 cut(s) 81
SatI GCNGC 2 cut(s) 105, 192
Sau96I GGNCC 1 cut(s) 199
SetI ASST 4 cut(s) 41, 100, 109, 166
SfaNI GCATC 1 cut(s) 147
SmiMI CAYNNNNRTG 1 cut(s) 81
Sse9I AATT 1 cut(s) 42
SsiI CCGC 1 cut(s) 189
SspMI CTAG 2 cut(s) 53, 147
StyI CCWWGG 2 cut(s) 57, 164
TaaI ACNGT 1 cut(s) 217
TasI AATT 1 cut(s) 42
TfiI GAWTC 1 cut(s) 178
TscAI CASTG 2 cut(s) 133, 222
TseI GCWGC 2 cut(s) 104, 191
TspDTI ATGAA 1 cut(s) 226
TspRI CASTG 2 cut(s) 133, 222
XmnI GAANNNNTTC 1 cut(s) 281
XspI CTAG 2 cut(s) 53, 147
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.