Rh2DG042600

Early nodulin-93-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0003255
Physical Location & Seq
Reverse (-)
2944893 .. 2949178
4286 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG042600.1

Sequence Viewer

Length: 348 bp
ATGAACTTTATTTCCTTTCTTGATTTTCTGAAGTTTAGAACAATTGTGAGAAGATCACTAGCTAGCCTAAGAATTTCTTCTGGAAGTTTAACCTTTAACTTTTTCAATGGTTCAGAGGGTGTAAAAGCTGGAGCTAAGGCAGCTGTTGTTGCTAGCATTGCCACTGCAATTCCAACTTTTGCTAGTGTGAGGATGCTGCCATGGGCAAAAGCCAATCTGAATCCCACTGCTCAGGCTCTCATAATTTCCACAGCGGCTGGAATGGCATATTTCATAGTGGCTGACAAGACCATTTTGGCAACCGCAAGGAAGAACTCATTCCACCAAGGATCTAACTATGAAGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ENOD93

Protein Analysis

115

Amino Acids

12.32

Weight (kDa)

10.71

Isoelectric Point (pI)

18.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ENOD93 PF03386 38 - 106 3e-36 Early nodulin 93 ENOD93 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 254, 303
AclWI GGATC 1 cut(s) 337
AcsI RAATTY 1 cut(s) 72
AcuI CTGAAG 1 cut(s) 50
AgsI TTSAA 1 cut(s) 106
AloI GAACNNNNNNTCC 1 cut(s) 28
AluBI AGCT 4 cut(s) 62, 128, 134, 143
AluI AGCT 4 cut(s) 62, 128, 134, 143
AlwI GGATC 1 cut(s) 337
AlwNI CAGNNNCTG 1 cut(s) 257
ApeKI GCWGC 2 cut(s) 140, 196
ApoI RAATTY 1 cut(s) 72
Asp700I GAANNNNTTC 2 cut(s) 76, 317
AsuNHI GCTAGC 2 cut(s) 62, 152
BbvI GCAGC 2 cut(s) 152, 183
BfaI CTAG 4 cut(s) 59, 63, 153, 183
BisI GCNGC 3 cut(s) 141, 197, 255
BlsI GCNGC 3 cut(s) 142, 198, 256
BmsI GCATC 1 cut(s) 183
BmtI GCTAGC 2 cut(s) 66, 156
BpmI CTGGAG 1 cut(s) 150
Bpu10I CCTNAGC 2 cut(s) 135, 231
BsaJI CCNNGG 2 cut(s) 200, 325
Bse3DI GCAATG 1 cut(s) 156
BseDI CCNNGG 2 cut(s) 200, 325
BseGI GGATG 1 cut(s) 198
BseMI GCAATG 1 cut(s) 156
BseMII CTCAG 1 cut(s) 245
BseXI GCAGC 2 cut(s) 152, 183
Bsp143I GATC 2 cut(s) 53, 329
Bsp19I CCATGG 1 cut(s) 200
BspACI CCGC 2 cut(s) 254, 303
BspCNI CTCAG 1 cut(s) 244
BspOI GCTAGC 2 cut(s) 66, 156
BspPI GGATC 1 cut(s) 337
BsrDI GCAATG 1 cut(s) 156
BssECI CCNNGG 2 cut(s) 200, 325
BssMI GATC 2 cut(s) 53, 329
BssT1I CCWWGG 2 cut(s) 200, 325
BstC8I GCNNGC 2 cut(s) 64, 154
BstDEI CTNAG 3 cut(s) 68, 135, 231
BstDSI CCRYGG 1 cut(s) 200
BstF5I GGATG 1 cut(s) 198
BstKTI GATC 2 cut(s) 56, 332
BstMBI GATC 2 cut(s) 53, 329
BstMWI GCNNNNNNNGC 4 cut(s) 140, 149, 158, 263
BstV1I GCAGC 2 cut(s) 152, 183
BstX2I RGATCY 1 cut(s) 329
BstYI RGATCY 1 cut(s) 329
BtgI CCRYGG 1 cut(s) 200
BtsCI GGATG 1 cut(s) 198
BtsI GCAGTG 2 cut(s) 162, 225
BtsIMutI CAGTG 2 cut(s) 162, 225
Cac8I GCNNGC 2 cut(s) 64, 154
CaiI CAGNNNCTG 1 cut(s) 257
CviAII CATG 1 cut(s) 201
CviJI RGCY 9 cut(s) 62, 66, 128, 134, 143, 212, 236, 257, 281
CviKI_1 RGCY 9 cut(s) 62, 66, 128, 134, 143, 212, 236, 257, 281
DdeI CTNAG 3 cut(s) 68, 135, 231
DpnI GATC 2 cut(s) 55, 331
DpnII GATC 2 cut(s) 53, 329
Eco130I CCWWGG 2 cut(s) 200, 325
Eco57I CTGAAG 1 cut(s) 50
EcoT14I CCWWGG 2 cut(s) 200, 325
ErhI CCWWGG 2 cut(s) 200, 325
FaeI CATG 1 cut(s) 204
FaiI YATR 6 cut(s) 202, 242, 268, 275, 339, 346
FalI AAGNNNNNCTT 2 cut(s) 61, 93
FatI CATG 1 cut(s) 200
Fnu4HI GCNGC 3 cut(s) 141, 197, 255
FokI GGATG 1 cut(s) 205
Fsp4HI GCNGC 3 cut(s) 141, 197, 255
FspBI CTAG 4 cut(s) 59, 63, 153, 183
GluI GCNGC 3 cut(s) 141, 197, 255
GsuI CTGGAG 1 cut(s) 150
Hin1II CATG 1 cut(s) 204
HinfI GANTC 1 cut(s) 220
Hpy188I TCNGA 3 cut(s) 30, 115, 219
Hpy188III TCNNGA 2 cut(s) 20, 81
HpyCH4V TGCA 1 cut(s) 167
HpyF10VI GCNNNNNNNGC 4 cut(s) 140, 149, 158, 263
HpyF3I CTNAG 3 cut(s) 68, 135, 231
Hsp92II CATG 1 cut(s) 204
Kzo9I GATC 2 cut(s) 53, 329
LmnI GCTCC 1 cut(s) 131
LpnPI CCDG 4 cut(s) 66, 114, 218, 243
Lsp1109I GCAGC 2 cut(s) 152, 183
LweI GCATC 1 cut(s) 183
MaeI CTAG 4 cut(s) 59, 63, 153, 183
MalI GATC 2 cut(s) 55, 331
MboI GATC 2 cut(s) 53, 329
MboII GAAGA 3 cut(s) 63, 69, 322
MfeI CAATTG 1 cut(s) 42
MflI RGATCY 1 cut(s) 329
MluCI AATT 4 cut(s) 42, 72, 168, 243
MmeI TCCRAC 1 cut(s) 197
MnlI CCTC 2 cut(s) 109, 183
MroXI GAANNNNTTC 2 cut(s) 76, 317
MseI TTAA 2 cut(s) 89, 96
MspA1I CMGCKG 2 cut(s) 143, 254
MunI CAATTG 1 cut(s) 42
MwoI GCNNNNNNNGC 4 cut(s) 140, 149, 158, 263
NcoI CCATGG 1 cut(s) 200
NdeII GATC 2 cut(s) 53, 329
NheI GCTAGC 2 cut(s) 62, 152
NlaIII CATG 1 cut(s) 204
PdmI GAANNNNTTC 2 cut(s) 76, 317
PfeI GAWTC 1 cut(s) 220
PkrI GCNGC 3 cut(s) 142, 198, 256
PstNI CAGNNNCTG 1 cut(s) 257
PsuI RGATCY 1 cut(s) 329
PvuII CAGCTG 1 cut(s) 143
SaqAI TTAA 2 cut(s) 89, 96
SatI GCNGC 3 cut(s) 141, 197, 255
Sau3AI GATC 2 cut(s) 53, 329
SetI ASST 5 cut(s) 64, 95, 130, 136, 145
SfaNI GCATC 1 cut(s) 183
Sse9I AATT 4 cut(s) 42, 72, 168, 243
SsiI CCGC 2 cut(s) 254, 303
SspMI CTAG 4 cut(s) 59, 63, 153, 183
StyI CCWWGG 2 cut(s) 200, 325
TasI AATT 4 cut(s) 42, 72, 168, 243
TauI GCSGC 1 cut(s) 257
TfiI GAWTC 1 cut(s) 220
Tru1I TTAA 2 cut(s) 89, 96
Tru9I TTAA 2 cut(s) 89, 96
TscAI CASTG 2 cut(s) 169, 232
TseI GCWGC 2 cut(s) 140, 196
TspDTI ATGAA 2 cut(s) 17, 262
TspRI CASTG 2 cut(s) 169, 232
XapI RAATTY 1 cut(s) 72
XmnI GAANNNNTTC 2 cut(s) 76, 317
XspI CTAG 4 cut(s) 59, 63, 153, 183
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.