Rh6CG478400

Early nodulin-93-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0003255
Physical Location & Seq
Reverse (-)
64631683 .. 64634194
2512 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG478400.1

Sequence Viewer

Length: 264 bp
ATGGGAATTCCATCGGAAATGAGGGACATGTGGGTGAACAACCGTCGAAACTCTCTGCTGATTCCTTCGCCATATGAAGATGAGATTCAACAGAAGGCCTTAAGGGCCAAAACTTGCACCCAAGAGGGCGCTCGCCAGGGATTCAAGGCAGCTTGCATTTGGGGTGCTGTCAGCGCTGTGCCTACAGTATGTGTTTCGTTGAACTCTTTAATCTGTTTTCGGCTGGAATTTGTTTCTTCCATTAGAGATTGGTCGTTTGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ENOD93

Protein Analysis

87

Amino Acids

9.83

Weight (kDa)

8.47

Isoelectric Point (pI)

45.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ENOD93 PF03386 33 - 64 3.5e-07 Early nodulin 93 ENOD93 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 2 cut(s) 6, 227
AfeI AGCGCT 1 cut(s) 175
AflII CTTAAG 1 cut(s) 100
AflIII ACRYGT 1 cut(s) 27
AgsI TTSAA 3 cut(s) 89, 145, 202
AjnI CCWGG 1 cut(s) 135
AluBI AGCT 1 cut(s) 152
AluI AGCT 1 cut(s) 152
Aor51HI AGCGCT 1 cut(s) 175
AoxI GGCC 2 cut(s) 96, 105
ApeKI GCWGC 1 cut(s) 149
ApoI RAATTY 2 cut(s) 6, 227
AspLEI GCGC 2 cut(s) 131, 176
AspS9I GGNCC 1 cut(s) 105
AsuHPI GGTGA 1 cut(s) 46
BbvI GCAGC 1 cut(s) 161
BccI CCATC 1 cut(s) 19
BciT130I CCWGG 1 cut(s) 137
BfmI CTRYAG 1 cut(s) 183
BfoI RGCGCY 2 cut(s) 132, 177
BfrI CTTAAG 1 cut(s) 100
BglI GCCNNNNNGGC 1 cut(s) 104
BisI GCNGC 1 cut(s) 150
BlsI GCNGC 1 cut(s) 151
Bme1390I CCNGG 1 cut(s) 137
BmgT120I GGNCC 1 cut(s) 105
BmrFI CCNGG 1 cut(s) 137
BsaJI CCNNGG 1 cut(s) 136
BseBI CCWGG 1 cut(s) 137
BseDI CCNNGG 1 cut(s) 136
BseXI GCAGC 1 cut(s) 161
BshFI GGCC 2 cut(s) 98, 107
BslFI GGGAC 1 cut(s) 38
BsmFI GGGAC 1 cut(s) 38
BsnI GGCC 2 cut(s) 98, 107
BspANI GGCC 2 cut(s) 98, 107
BspTI CTTAAG 1 cut(s) 100
BssECI CCNNGG 1 cut(s) 136
Bst2UI CCWGG 1 cut(s) 137
Bst4CI ACNGT 2 cut(s) 44, 187
BstAFI CTTAAG 1 cut(s) 100
BstC8I GCNNGC 2 cut(s) 133, 154
BstH2I RGCGCY 2 cut(s) 132, 177
BstHHI GCGC 2 cut(s) 131, 176
BstMWI GCNNNNNNNGC 2 cut(s) 104, 173
BstNI CCWGG 1 cut(s) 137
BstNSI RCATGY 1 cut(s) 31
BstSCI CCNGG 1 cut(s) 135
BstSFI CTRYAG 1 cut(s) 183
BstV1I GCAGC 1 cut(s) 161
BsuRI GGCC 2 cut(s) 98, 107
Cac8I GCNNGC 2 cut(s) 133, 154
CfoI GCGC 2 cut(s) 131, 176
Cfr13I GGNCC 1 cut(s) 105
CviAII CATG 1 cut(s) 28
CviJI RGCY 4 cut(s) 98, 107, 152, 223
CviKI_1 RGCY 4 cut(s) 98, 107, 152, 223
Eco147I AGGCCT 1 cut(s) 98
Eco47III AGCGCT 1 cut(s) 175
EcoRI GAATTC 1 cut(s) 6
EcoRII CCWGG 1 cut(s) 135
FaeI CATG 1 cut(s) 31
FaiI YATR 4 cut(s) 29, 73, 75, 190
FaqI GGGAC 1 cut(s) 38
FatI CATG 1 cut(s) 27
FauNDI CATATG 1 cut(s) 73
Fnu4HI GCNGC 1 cut(s) 150
Fsp4HI GCNGC 1 cut(s) 150
GlaI GCGC 2 cut(s) 130, 175
GluI GCNGC 1 cut(s) 150
HaeII RGCGCY 2 cut(s) 132, 177
HaeIII GGCC 2 cut(s) 98, 107
HhaI GCGC 2 cut(s) 131, 176
Hin1II CATG 1 cut(s) 31
Hin6I GCGC 2 cut(s) 129, 174
HinP1I GCGC 2 cut(s) 129, 174
HinfI GANTC 3 cut(s) 61, 85, 141
HphI GGTGA 1 cut(s) 46
Hpy166II GTNNAC 1 cut(s) 37
Hpy188I TCNGA 1 cut(s) 16
Hpy8I GTNNAC 1 cut(s) 37
Hpy99I CGWCG 1 cut(s) 48
HpyAV CCTTC 2 cut(s) 75, 88
HpyCH4III ACNGT 2 cut(s) 44, 187
HpyCH4V TGCA 2 cut(s) 117, 156
HpyF10VI GCNNNNNNNGC 2 cut(s) 104, 173
Hsp92II CATG 1 cut(s) 31
HspAI GCGC 2 cut(s) 129, 174
LpnPI CCDG 3 cut(s) 122, 149, 209
Lsp1109I GCAGC 1 cut(s) 161
MboII GAAGA 2 cut(s) 89, 228
MluCI AATT 2 cut(s) 6, 227
MnlI CCTC 2 cut(s) 15, 118
MseI TTAA 2 cut(s) 101, 209
MslI CAYNNNNRTG 1 cut(s) 32
MspCI CTTAAG 1 cut(s) 100
MspR9I CCNGG 1 cut(s) 137
MvaI CCWGG 1 cut(s) 137
MwoI GCNNNNNNNGC 2 cut(s) 104, 173
NdeI CATATG 1 cut(s) 73
NlaIII CATG 1 cut(s) 31
NspI RCATGY 1 cut(s) 31
PceI AGGCCT 1 cut(s) 98
PciI ACATGT 1 cut(s) 27
PfeI GAWTC 3 cut(s) 61, 85, 141
PkrI GCNGC 1 cut(s) 151
PscI ACATGT 1 cut(s) 27
Psp6I CCWGG 1 cut(s) 135
PspGI CCWGG 1 cut(s) 135
PspPI GGNCC 1 cut(s) 105
RseI CAYNNNNRTG 1 cut(s) 32
SaqAI TTAA 2 cut(s) 101, 209
SatI GCNGC 1 cut(s) 150
Sau96I GGNCC 1 cut(s) 105
ScrFI CCNGG 1 cut(s) 137
SetI ASST 1 cut(s) 154
SfcI CTRYAG 1 cut(s) 183
SfiI GGCCNNNNNGGCC 1 cut(s) 104
SgeI CNNG 9 cut(s) 40, 126, 134, 144, 148, 149, 157, 165, 236
SmiMI CAYNNNNRTG 1 cut(s) 32
SmlI CTYRAG 1 cut(s) 100
SmoI CTYRAG 1 cut(s) 100
Sse9I AATT 2 cut(s) 6, 227
SseBI AGGCCT 1 cut(s) 98
StuI AGGCCT 1 cut(s) 98
StyD4I CCNGG 1 cut(s) 135
TaaI ACNGT 2 cut(s) 44, 187
TaqI TCGA 1 cut(s) 46
TasI AATT 2 cut(s) 6, 227
TfiI GAWTC 3 cut(s) 61, 85, 141
Tru1I TTAA 2 cut(s) 101, 209
Tru9I TTAA 2 cut(s) 101, 209
TseI GCWGC 1 cut(s) 149
TspDTI ATGAA 1 cut(s) 90
Vha464I CTTAAG 1 cut(s) 100
XapI RAATTY 2 cut(s) 6, 227
XceI RCATGY 1 cut(s) 31
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.