RchiOBHm_Chr2g0089171

Early nodulin-93-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0003255
Physical Location & Seq
Reverse (-)
3440754 .. 3442212
1459 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ46447

Sequence Viewer

Length: 318 bp
ATGGCAAAGAATGTGGCTCAGTCTCCCATGGAGAAACGCAGCCTGACGTCATTTGACCAGCAGTTGGCTAAGCGCTGTTCTCATGAGGGTGTAAAAGCTGGAGCTAAGGCAGCTGTTGTTGCTAGCATTGCCACTGCAATTCCAACTTTTGCTAGTGTGAGGATGCTGCCATGGGCAAAAGCCAATCTGAATCCCACTGCTCAGGCTCTCATAATTTCCACAGCGGCTGGAATGGCATATTTCATAGTGGCTGACAAGACCATTTTGGCAACCGCAAGGAAGAACTCATTCCACCAAGGATCTAACTATGAAGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ENOD93

Protein Analysis

105

Amino Acids

11.12

Weight (kDa)

10.01

Isoelectric Point (pI)

31.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ENOD93 PF03386 22 - 96 1.3e-40 Early nodulin 93 ENOD93 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 50
AccB7I CCANNNNNTGG 1 cut(s) 64
AciI CCGC 2 cut(s) 224, 273
AclWI GGATC 1 cut(s) 307
AcyI GRCGYC 1 cut(s) 47
AfeI AGCGCT 1 cut(s) 74
AfiI CCNNNNNNNGG 1 cut(s) 64
AluBI AGCT 3 cut(s) 98, 104, 113
AluI AGCT 3 cut(s) 98, 104, 113
Alw26I GTCTC 1 cut(s) 27
AlwI GGATC 1 cut(s) 307
AlwNI CAGNNNCTG 1 cut(s) 227
Aor51HI AGCGCT 1 cut(s) 74
ApeKI GCWGC 3 cut(s) 39, 110, 166
Asp700I GAANNNNTTC 1 cut(s) 287
AspLEI GCGC 1 cut(s) 75
AsuNHI GCTAGC 1 cut(s) 122
BbvI GCAGC 3 cut(s) 51, 122, 153
BcoDI GTCTC 1 cut(s) 27
BfaI CTAG 2 cut(s) 123, 153
BfoI RGCGCY 1 cut(s) 76
BisI GCNGC 4 cut(s) 40, 111, 167, 225
BlpI GCTNAGC 1 cut(s) 69
BlsI GCNGC 4 cut(s) 41, 112, 168, 226
BmsI GCATC 1 cut(s) 153
BmtI GCTAGC 1 cut(s) 126
BpmI CTGGAG 1 cut(s) 120
Bpu10I CCTNAGC 2 cut(s) 105, 201
Bpu1102I GCTNAGC 1 cut(s) 69
BsaHI GRCGYC 1 cut(s) 47
BsaJI CCNNGG 3 cut(s) 27, 170, 295
Bsc4I CCNNNNNNNGG 1 cut(s) 64
Bse3DI GCAATG 1 cut(s) 126
BseDI CCNNGG 3 cut(s) 27, 170, 295
BseGI GGATG 1 cut(s) 168
BseLI CCNNNNNNNGG 1 cut(s) 64
BseMI GCAATG 1 cut(s) 126
BseMII CTCAG 2 cut(s) 32, 215
BseXI GCAGC 3 cut(s) 51, 122, 153
BslI CCNNNNNNNGG 1 cut(s) 64
BsmAI GTCTC 1 cut(s) 27
Bsp143I GATC 1 cut(s) 299
Bsp1720I GCTNAGC 1 cut(s) 69
Bsp19I CCATGG 2 cut(s) 27, 170
BspACI CCGC 2 cut(s) 224, 273
BspCNI CTCAG 2 cut(s) 31, 214
BspHI TCATGA 1 cut(s) 82
BspOI GCTAGC 1 cut(s) 126
BspPI GGATC 1 cut(s) 307
BsrDI GCAATG 1 cut(s) 126
BssECI CCNNGG 3 cut(s) 27, 170, 295
BssMI GATC 1 cut(s) 299
BssNI GRCGYC 1 cut(s) 47
BssT1I CCWWGG 3 cut(s) 27, 170, 295
BstACI GRCGYC 1 cut(s) 47
BstC8I GCNNGC 1 cut(s) 124
BstDEI CTNAG 4 cut(s) 18, 69, 105, 201
BstDSI CCRYGG 2 cut(s) 27, 170
BstF5I GGATG 1 cut(s) 168
BstH2I RGCGCY 1 cut(s) 76
BstHHI GCGC 1 cut(s) 75
BstKTI GATC 1 cut(s) 302
BstMAI GTCTC 1 cut(s) 27
BstMBI GATC 1 cut(s) 299
BstMWI GCNNNNNNNGC 4 cut(s) 110, 119, 128, 233
BstV1I GCAGC 3 cut(s) 51, 122, 153
BstX2I RGATCY 1 cut(s) 299
BstYI RGATCY 1 cut(s) 299
BtgI CCRYGG 2 cut(s) 27, 170
BtsCI GGATG 1 cut(s) 168
BtsI GCAGTG 2 cut(s) 132, 195
BtsIMutI CAGTG 2 cut(s) 132, 195
Cac8I GCNNGC 1 cut(s) 124
CaiI CAGNNNCTG 1 cut(s) 227
CciI TCATGA 1 cut(s) 82
CfoI GCGC 1 cut(s) 75
CspCI CAANNNNNGTGG 1 cut(s) 29
CviAII CATG 3 cut(s) 28, 83, 171
DdeI CTNAG 4 cut(s) 18, 69, 105, 201
DpnI GATC 1 cut(s) 301
DpnII GATC 1 cut(s) 299
Eco130I CCWWGG 3 cut(s) 27, 170, 295
Eco47III AGCGCT 1 cut(s) 74
EcoT14I CCWWGG 3 cut(s) 27, 170, 295
ErhI CCWWGG 3 cut(s) 27, 170, 295
FaeI CATG 3 cut(s) 31, 86, 174
FaiI YATR 8 cut(s) 29, 84, 172, 212, 238, 245, 309, 316
FatI CATG 3 cut(s) 27, 82, 170
Fnu4HI GCNGC 4 cut(s) 40, 111, 167, 225
FokI GGATG 1 cut(s) 175
Fsp4HI GCNGC 4 cut(s) 40, 111, 167, 225
FspBI CTAG 2 cut(s) 123, 153
GlaI GCGC 1 cut(s) 74
GluI GCNGC 4 cut(s) 40, 111, 167, 225
GsuI CTGGAG 1 cut(s) 120
HaeII RGCGCY 1 cut(s) 76
HhaI GCGC 1 cut(s) 75
Hin1I GRCGYC 1 cut(s) 47
Hin1II CATG 3 cut(s) 31, 86, 174
Hin6I GCGC 1 cut(s) 73
HinP1I GCGC 1 cut(s) 73
HinfI GANTC 1 cut(s) 190
Hpy188I TCNGA 1 cut(s) 189
Hpy188III TCNNGA 1 cut(s) 83
HpyCH4IV ACGT 1 cut(s) 47
HpyCH4V TGCA 1 cut(s) 137
HpyF10VI GCNNNNNNNGC 4 cut(s) 110, 119, 128, 233
HpyF3I CTNAG 4 cut(s) 18, 69, 105, 201
HpySE526I ACGT 1 cut(s) 47
Hsp92I GRCGYC 1 cut(s) 47
Hsp92II CATG 3 cut(s) 31, 86, 174
HspAI GCGC 1 cut(s) 73
Kzo9I GATC 1 cut(s) 299
LmnI GCTCC 1 cut(s) 101
LpnPI CCDG 5 cut(s) 56, 71, 84, 188, 213
Lsp1109I GCAGC 3 cut(s) 51, 122, 153
LweI GCATC 1 cut(s) 153
MaeI CTAG 2 cut(s) 123, 153
MaeII ACGT 1 cut(s) 47
MalI GATC 1 cut(s) 301
MboI GATC 1 cut(s) 299
MboII GAAGA 1 cut(s) 292
MflI RGATCY 1 cut(s) 299
MluCI AATT 2 cut(s) 138, 213
MmeI TCCRAC 1 cut(s) 167
MnlI CCTC 2 cut(s) 79, 153
MroXI GAANNNNTTC 1 cut(s) 287
MslI CAYNNNNRTG 1 cut(s) 87
MspA1I CMGCKG 2 cut(s) 113, 224
MwoI GCNNNNNNNGC 4 cut(s) 110, 119, 128, 233
NcoI CCATGG 2 cut(s) 27, 170
NdeII GATC 1 cut(s) 299
NheI GCTAGC 1 cut(s) 122
NlaIII CATG 3 cut(s) 31, 86, 174
PagI TCATGA 1 cut(s) 82
PdmI GAANNNNTTC 1 cut(s) 287
PfeI GAWTC 1 cut(s) 190
PflMI CCANNNNNTGG 1 cut(s) 64
PkrI GCNGC 4 cut(s) 41, 112, 168, 226
PstNI CAGNNNCTG 1 cut(s) 227
PsuI RGATCY 1 cut(s) 299
PvuII CAGCTG 1 cut(s) 113
RseI CAYNNNNRTG 1 cut(s) 87
SatI GCNGC 4 cut(s) 40, 111, 167, 225
Sau3AI GATC 1 cut(s) 299
SetI ASST 4 cut(s) 50, 100, 106, 115
SfaNI GCATC 1 cut(s) 153
SmiMI CAYNNNNRTG 1 cut(s) 87
Sse9I AATT 2 cut(s) 138, 213
SsiI CCGC 2 cut(s) 224, 273
SspMI CTAG 2 cut(s) 123, 153
StyI CCWWGG 3 cut(s) 27, 170, 295
TaiI ACGT 1 cut(s) 50
TasI AATT 2 cut(s) 138, 213
TauI GCSGC 1 cut(s) 227
TfiI GAWTC 1 cut(s) 190
TscAI CASTG 2 cut(s) 139, 202
TseI GCWGC 3 cut(s) 39, 110, 166
TspDTI ATGAA 1 cut(s) 232
TspRI CASTG 2 cut(s) 139, 202
Van91I CCANNNNNTGG 1 cut(s) 64
XmnI GAANNNNTTC 1 cut(s) 287
XspI CTAG 2 cut(s) 123, 153
ZraI GACGTC 1 cut(s) 48
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.