Rroxscaffold_2G00152190

Early nodulin-93-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0003255
Physical Location & Seq
Forward (+)
89255131 .. 89256526
1396 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00152190.1

Sequence Viewer

Length: 441 bp
ATGCGTAACTTTACCATTCATCCAAAGTTTTCAGGAAATAGCTTCTCAAAATTTCATCCCAGCTCTTCTAACTTCTATCATTCCATTTATTCATCCACTACTATACGTAGTAATACTCTTGCAATGGCAAAGAATGTGGCTCAGTCTCCCATGGAGAAACGCAGCCTGACGTCATTTGACCAGCAGTTGGCTAAGCGCTGTTCTCATGAGGGTGTAAAAGCTGGAGCTAAGGCAGCTGTTGTTGCTAGCATTGCCACTGCAATTCCAACTTTTGCTAGTGTGAGGATGCTGCCATGGGCAAAAGCCAATCTGAATCCCACTGCTCAGGCTCTCATAATCTCCACAGCGGCTGGAATGGCATATTTCATAGTGGCTGACAAGACCATTTTGGCAACCGCAAGGAAGAACTCATTCCATCAAGGATCTAACTATGAAGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ENOD93

Protein Analysis

146

Amino Acids

15.8

Weight (kDa)

10.25

Isoelectric Point (pI)

34.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ENOD93 PF03386 63 - 137 6.5e-40 Early nodulin 93 ENOD93 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 173
AccB7I CCANNNNNTGG 1 cut(s) 187
AciI CCGC 2 cut(s) 347, 396
AclWI GGATC 1 cut(s) 430
AcsI RAATTY 1 cut(s) 50
AcyI GRCGYC 1 cut(s) 170
AfeI AGCGCT 1 cut(s) 197
AfiI CCNNNNNNNGG 1 cut(s) 187
AluBI AGCT 5 cut(s) 42, 63, 221, 227, 236
AluI AGCT 5 cut(s) 42, 63, 221, 227, 236
Alw26I GTCTC 1 cut(s) 150
AlwI GGATC 1 cut(s) 430
AlwNI CAGNNNCTG 1 cut(s) 350
Aor51HI AGCGCT 1 cut(s) 197
ApeKI GCWGC 3 cut(s) 162, 233, 289
ApoI RAATTY 1 cut(s) 50
Asp700I GAANNNNTTC 1 cut(s) 410
AspLEI GCGC 1 cut(s) 198
AsuNHI GCTAGC 1 cut(s) 245
BbvI GCAGC 3 cut(s) 174, 245, 276
BccI CCATC 1 cut(s) 423
BcoDI GTCTC 1 cut(s) 150
BfaI CTAG 2 cut(s) 246, 276
BfoI RGCGCY 1 cut(s) 199
BisI GCNGC 4 cut(s) 163, 234, 290, 348
BlpI GCTNAGC 1 cut(s) 192
BlsI GCNGC 4 cut(s) 164, 235, 291, 349
BmsI GCATC 1 cut(s) 276
BmtI GCTAGC 1 cut(s) 249
BpmI CTGGAG 1 cut(s) 243
Bpu10I CCTNAGC 2 cut(s) 228, 324
Bpu1102I GCTNAGC 1 cut(s) 192
BsaAI YACGTR 1 cut(s) 107
BsaHI GRCGYC 1 cut(s) 170
BsaJI CCNNGG 2 cut(s) 150, 293
Bsc4I CCNNNNNNNGG 1 cut(s) 187
Bse3DI GCAATG 2 cut(s) 129, 249
BseDI CCNNGG 2 cut(s) 150, 293
BseGI GGATG 4 cut(s) 19, 55, 92, 291
BseLI CCNNNNNNNGG 1 cut(s) 187
BseMI GCAATG 2 cut(s) 129, 249
BseMII CTCAG 2 cut(s) 155, 338
BseXI GCAGC 3 cut(s) 174, 245, 276
BseYI CCCAGC 1 cut(s) 59
BslI CCNNNNNNNGG 1 cut(s) 187
BsmAI GTCTC 1 cut(s) 150
Bsp143I GATC 1 cut(s) 422
Bsp1720I GCTNAGC 1 cut(s) 192
Bsp19I CCATGG 2 cut(s) 150, 293
BspACI CCGC 2 cut(s) 347, 396
BspCNI CTCAG 2 cut(s) 154, 337
BspHI TCATGA 1 cut(s) 205
BspOI GCTAGC 1 cut(s) 249
BspPI GGATC 1 cut(s) 430
BspQI GCTCTTC 1 cut(s) 70
BsrDI GCAATG 2 cut(s) 129, 249
BssECI CCNNGG 2 cut(s) 150, 293
BssMI GATC 1 cut(s) 422
BssNI GRCGYC 1 cut(s) 170
BssT1I CCWWGG 2 cut(s) 150, 293
Bst6I CTCTTC 1 cut(s) 70
BstACI GRCGYC 1 cut(s) 170
BstBAI YACGTR 1 cut(s) 107
BstC8I GCNNGC 1 cut(s) 247
BstDEI CTNAG 4 cut(s) 141, 192, 228, 324
BstDSI CCRYGG 2 cut(s) 150, 293
BstF5I GGATG 4 cut(s) 19, 55, 92, 291
BstH2I RGCGCY 1 cut(s) 199
BstHHI GCGC 1 cut(s) 198
BstKTI GATC 1 cut(s) 425
BstMAI GTCTC 1 cut(s) 150
BstMBI GATC 1 cut(s) 422
BstMWI GCNNNNNNNGC 4 cut(s) 233, 242, 251, 356
BstSNI TACGTA 1 cut(s) 107
BstV1I GCAGC 3 cut(s) 174, 245, 276
BstX2I RGATCY 1 cut(s) 422
BstYI RGATCY 1 cut(s) 422
BtgI CCRYGG 2 cut(s) 150, 293
BtsCI GGATG 4 cut(s) 19, 55, 92, 291
BtsI GCAGTG 2 cut(s) 255, 318
BtsIMutI CAGTG 2 cut(s) 255, 318
Cac8I GCNNGC 1 cut(s) 247
CaiI CAGNNNCTG 1 cut(s) 350
CciI TCATGA 1 cut(s) 205
CfoI GCGC 1 cut(s) 198
CspCI CAANNNNNGTGG 2 cut(s) 117, 152
CviAII CATG 3 cut(s) 151, 206, 294
DdeI CTNAG 4 cut(s) 141, 192, 228, 324
DpnI GATC 1 cut(s) 424
DpnII GATC 1 cut(s) 422
Eam1104I CTCTTC 1 cut(s) 70
EarI CTCTTC 1 cut(s) 70
Eco105I TACGTA 1 cut(s) 107
Eco130I CCWWGG 2 cut(s) 150, 293
Eco47III AGCGCT 1 cut(s) 197
EcoT14I CCWWGG 2 cut(s) 150, 293
ErhI CCWWGG 2 cut(s) 150, 293
FaeI CATG 3 cut(s) 154, 209, 297
FaiI YATR 9 cut(s) 104, 152, 207, 295, 335, 361, 368, 432, 439
FatI CATG 3 cut(s) 150, 205, 293
Fnu4HI GCNGC 4 cut(s) 163, 234, 290, 348
FokI GGATG 4 cut(s) 6, 42, 79, 298
Fsp4HI GCNGC 4 cut(s) 163, 234, 290, 348
FspBI CTAG 2 cut(s) 246, 276
GlaI GCGC 1 cut(s) 197
GluI GCNGC 4 cut(s) 163, 234, 290, 348
GsaI CCCAGC 1 cut(s) 63
GsuI CTGGAG 1 cut(s) 243
HaeII RGCGCY 1 cut(s) 199
HhaI GCGC 1 cut(s) 198
Hin1I GRCGYC 1 cut(s) 170
Hin1II CATG 3 cut(s) 154, 209, 297
Hin6I GCGC 1 cut(s) 196
HinP1I GCGC 1 cut(s) 196
HinfI GANTC 1 cut(s) 313
Hpy188I TCNGA 1 cut(s) 312
Hpy188III TCNNGA 2 cut(s) 33, 206
HpyCH4IV ACGT 2 cut(s) 106, 170
HpyCH4V TGCA 2 cut(s) 122, 260
HpyF10VI GCNNNNNNNGC 4 cut(s) 233, 242, 251, 356
HpyF3I CTNAG 4 cut(s) 141, 192, 228, 324
HpySE526I ACGT 2 cut(s) 106, 170
Hsp92I GRCGYC 1 cut(s) 170
Hsp92II CATG 3 cut(s) 154, 209, 297
HspAI GCGC 1 cut(s) 196
Kzo9I GATC 1 cut(s) 422
LguI GCTCTTC 1 cut(s) 70
LmnI GCTCC 1 cut(s) 224
LpnPI CCDG 7 cut(s) 18, 73, 179, 194, 207, 311, 336
Lsp1109I GCAGC 3 cut(s) 174, 245, 276
LweI GCATC 1 cut(s) 276
MaeI CTAG 2 cut(s) 246, 276
MaeII ACGT 2 cut(s) 106, 170
MaeIII GTNAC 1 cut(s) 5
MalI GATC 1 cut(s) 424
MboI GATC 1 cut(s) 422
MboII GAAGA 2 cut(s) 57, 415
MflI RGATCY 1 cut(s) 422
MluCI AATT 2 cut(s) 50, 261
MmeI TCCRAC 1 cut(s) 290
MnlI CCTC 2 cut(s) 202, 276
MroXI GAANNNNTTC 1 cut(s) 410
MslI CAYNNNNRTG 1 cut(s) 210
MspA1I CMGCKG 2 cut(s) 236, 347
MwoI GCNNNNNNNGC 4 cut(s) 233, 242, 251, 356
NcoI CCATGG 2 cut(s) 150, 293
NdeII GATC 1 cut(s) 422
NheI GCTAGC 1 cut(s) 245
NlaIII CATG 3 cut(s) 154, 209, 297
PagI TCATGA 1 cut(s) 205
PciSI GCTCTTC 1 cut(s) 70
PdmI GAANNNNTTC 1 cut(s) 410
PfeI GAWTC 1 cut(s) 313
PflMI CCANNNNNTGG 1 cut(s) 187
PkrI GCNGC 4 cut(s) 164, 235, 291, 349
Ppu21I YACGTR 1 cut(s) 107
PspFI CCCAGC 1 cut(s) 59
PstNI CAGNNNCTG 1 cut(s) 350
PsuI RGATCY 1 cut(s) 422
PvuII CAGCTG 1 cut(s) 236
RseI CAYNNNNRTG 1 cut(s) 210
SapI GCTCTTC 1 cut(s) 70
SatI GCNGC 4 cut(s) 163, 234, 290, 348
Sau3AI GATC 1 cut(s) 422
SetI ASST 7 cut(s) 44, 65, 109, 173, 223, 229, 238
SfaNI GCATC 1 cut(s) 276
SmiMI CAYNNNNRTG 1 cut(s) 210
SnaBI TACGTA 1 cut(s) 107
Sse9I AATT 2 cut(s) 50, 261
SsiI CCGC 2 cut(s) 347, 396
SspMI CTAG 2 cut(s) 246, 276
StyI CCWWGG 2 cut(s) 150, 293
TaiI ACGT 2 cut(s) 109, 173
TasI AATT 2 cut(s) 50, 261
TauI GCSGC 1 cut(s) 350
TfiI GAWTC 1 cut(s) 313
TscAI CASTG 2 cut(s) 262, 325
TseI GCWGC 3 cut(s) 162, 233, 289
TspDTI ATGAA 4 cut(s) 8, 44, 81, 355
TspRI CASTG 2 cut(s) 262, 325
Van91I CCANNNNNTGG 1 cut(s) 187
XapI RAATTY 1 cut(s) 50
XmnI GAANNNNTTC 1 cut(s) 410
XspI CTAG 2 cut(s) 246, 276
ZraI GACGTC 1 cut(s) 171
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.