MD02G1036100.v1.1

Early nodulin-93-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0003255
Physical Location & Seq
Reverse (-)
2737529 .. 2741238
3710 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1036100.v1.1.491

Sequence Viewer

Length: 339 bp
ATGAAGGCGTCAATAAAGTCTCTAACAATGGCAAGAAATCTGCCTCAGTCTCCCTATGAGAAGACCTCAATTGACCAGTTGGCCTTGGCTAAGCACTGCTCTCGCGAAGGTGTAAAAGCGGGAGCTAAGGCAGCTGTTGTTGCTACCATTGCCACAGCCATTCCAACTCTGGTTAGTGTGAGGATGCTGCCTTGGGCAAGAACCAATCTCAACCCCACTGCTCAGGCCCTCATAATCTCCACAGTGGCTGGAATGGCATACTTCATCGTGGCTGACAAGACTGTTTTGGCAACGGCAAGAAGGAACTCATTCAATCAAGTGGCTCACCATGAAGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ENOD93

Protein Analysis

113

Amino Acids

11.96

Weight (kDa)

10.29

Isoelectric Point (pI)

23.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ENOD93 PF03386 26 - 103 1.9e-39 Early nodulin 93 ENOD93 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

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Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 105
AciI CCGC 1 cut(s) 119
AcyI GRCGYC 1 cut(s) 8
AgsI TTSAA 1 cut(s) 313
AluBI AGCT 2 cut(s) 125, 134
AluI AGCT 2 cut(s) 125, 134
Alw26I GTCTC 2 cut(s) 24, 54
AlwNI CAGNNNCTG 1 cut(s) 248
AoxI GGCC 2 cut(s) 81, 225
ApeKI GCWGC 2 cut(s) 131, 187
Asp700I GAANNNNTTC 1 cut(s) 308
AspS9I GGNCC 1 cut(s) 226
AsuHPI GGTGA 1 cut(s) 317
BbsI GAAGAC 1 cut(s) 68
BbvI GCAGC 2 cut(s) 143, 174
BceAI ACGGC 1 cut(s) 309
BcgI CGANNNNNNTGC 2 cut(s) 83, 117
BcoDI GTCTC 2 cut(s) 24, 54
BisI GCNGC 2 cut(s) 132, 188
BlpI GCTNAGC 1 cut(s) 90
BlsI GCNGC 2 cut(s) 133, 189
BmgT120I GGNCC 1 cut(s) 226
BmsI GCATC 1 cut(s) 174
BpiI GAAGAC 1 cut(s) 68
BplI GAGNNNNNCTC 2 cut(s) 50, 82
Bpu10I CCTNAGC 2 cut(s) 126, 222
Bpu1102I GCTNAGC 1 cut(s) 90
BsaHI GRCGYC 1 cut(s) 8
BsaJI CCNNGG 2 cut(s) 84, 191
Bse1I ACTGG 1 cut(s) 76
Bse3DI GCAATG 1 cut(s) 147
BseDI CCNNGG 2 cut(s) 84, 191
BseGI GGATG 1 cut(s) 189
BseMI GCAATG 1 cut(s) 147
BseMII CTCAG 2 cut(s) 59, 236
BseNI ACTGG 1 cut(s) 76
BseXI GCAGC 2 cut(s) 143, 174
Bsh1236I CGCG 1 cut(s) 105
BshFI GGCC 2 cut(s) 83, 227
BsmAI GTCTC 2 cut(s) 24, 54
BsnI GGCC 2 cut(s) 83, 227
Bsp1720I GCTNAGC 1 cut(s) 90
Bsp68I TCGCGA 1 cut(s) 105
BspACI CCGC 1 cut(s) 119
BspANI GGCC 2 cut(s) 83, 227
BspCNI CTCAG 2 cut(s) 58, 235
BspFNI CGCG 1 cut(s) 105
BsrDI GCAATG 1 cut(s) 147
BsrI ACTGG 1 cut(s) 76
BssECI CCNNGG 2 cut(s) 84, 191
BssNI GRCGYC 1 cut(s) 8
BssT1I CCWWGG 2 cut(s) 84, 191
Bst4CI ACNGT 2 cut(s) 244, 283
BstACI GRCGYC 1 cut(s) 8
BstDEI CTNAG 4 cut(s) 45, 90, 126, 222
BstF5I GGATG 1 cut(s) 189
BstFNI CGCG 1 cut(s) 105
BstMAI GTCTC 2 cut(s) 24, 54
BstMWI GCNNNNNNNGC 4 cut(s) 131, 140, 149, 254
BstUI CGCG 1 cut(s) 105
BstV1I GCAGC 2 cut(s) 143, 174
BstV2I GAAGAC 1 cut(s) 68
BsuRI GGCC 2 cut(s) 83, 227
BtsCI GGATG 1 cut(s) 189
BtsI GCAGTG 2 cut(s) 94, 216
BtsIMutI CAGTG 3 cut(s) 94, 216, 249
BtuMI TCGCGA 1 cut(s) 105
CaiI CAGNNNCTG 1 cut(s) 248
Cfr13I GGNCC 1 cut(s) 226
CviAII CATG 2 cut(s) 329, 336
CviJI RGCY 9 cut(s) 83, 89, 125, 134, 158, 227, 248, 272, 323
CviKI_1 RGCY 9 cut(s) 83, 89, 125, 134, 158, 227, 248, 272, 323
DdeI CTNAG 4 cut(s) 45, 90, 126, 222
Eco130I CCWWGG 2 cut(s) 84, 191
EcoO109I RGGNCCY 1 cut(s) 226
EcoT14I CCWWGG 2 cut(s) 84, 191
ErhI CCWWGG 2 cut(s) 84, 191
FaeI CATG 2 cut(s) 332, 339
FaiI YATR 5 cut(s) 57, 233, 259, 330, 337
FatI CATG 2 cut(s) 328, 335
FauI CCCGC 1 cut(s) 112
Fnu4HI GCNGC 2 cut(s) 132, 188
FokI GGATG 1 cut(s) 196
Fsp4HI GCNGC 2 cut(s) 132, 188
GluI GCNGC 2 cut(s) 132, 188
HaeIII GGCC 2 cut(s) 83, 227
Hin1I GRCGYC 1 cut(s) 8
Hin1II CATG 2 cut(s) 332, 339
HphI GGTGA 1 cut(s) 317
Hpy188III TCNNGA 1 cut(s) 104
HpyAV CCTTC 2 cut(s) 101, 294
HpyCH4III ACNGT 2 cut(s) 244, 283
HpyF10VI GCNNNNNNNGC 4 cut(s) 131, 140, 149, 254
HpyF3I CTNAG 4 cut(s) 45, 90, 126, 222
Hsp92I GRCGYC 1 cut(s) 8
Hsp92II CATG 2 cut(s) 332, 339
LmnI GCTCC 1 cut(s) 122
LpnPI CCDG 4 cut(s) 89, 155, 209, 234
Lsp1109I GCAGC 2 cut(s) 143, 174
LweI GCATC 1 cut(s) 174
MboII GAAGA 1 cut(s) 73
MfeI CAATTG 1 cut(s) 69
MluCI AATT 1 cut(s) 69
MmeI TCCRAC 1 cut(s) 188
MnlI CCTC 4 cut(s) 54, 76, 174, 239
MroXI GAANNNNTTC 1 cut(s) 308
MspA1I CMGCKG 1 cut(s) 134
MunI CAATTG 1 cut(s) 69
MvnI CGCG 1 cut(s) 105
MwoI GCNNNNNNNGC 4 cut(s) 131, 140, 149, 254
NlaIII CATG 2 cut(s) 332, 339
NruI TCGCGA 1 cut(s) 105
PdmI GAANNNNTTC 1 cut(s) 308
PkrI GCNGC 2 cut(s) 133, 189
PspPI GGNCC 1 cut(s) 226
PstNI CAGNNNCTG 1 cut(s) 248
PvuII CAGCTG 1 cut(s) 134
RruI TCGCGA 1 cut(s) 105
SatI GCNGC 2 cut(s) 132, 188
Sau96I GGNCC 1 cut(s) 226
SetI ASST 4 cut(s) 68, 112, 127, 136
SfaNI GCATC 1 cut(s) 174
Sse9I AATT 1 cut(s) 69
SsiI CCGC 1 cut(s) 119
StyI CCWWGG 2 cut(s) 84, 191
TaaI ACNGT 2 cut(s) 244, 283
TasI AATT 1 cut(s) 69
TscAI CASTG 3 cut(s) 101, 223, 249
TseI GCWGC 2 cut(s) 131, 187
TspDTI ATGAA 2 cut(s) 17, 253
TspRI CASTG 3 cut(s) 101, 223, 249
XcmI CCANNNNNNNNNTGG 1 cut(s) 166
XmnI GAANNNNTTC 1 cut(s) 308
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.