FvH4_1g20831

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0005517
Physical Location & Seq
Reverse (-)
12986201 .. 12988455
2255 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g20831.t1

Sequence Viewer

Length: 816 bp
ATGCTGACTGATGAAGGGGAATCACGAAAACGAAAACGAAATGCTTCTTGTTGCACCTGTCGACCCCGACGCCCTGCCAAAACTAGGAAACTTGCTTTACCAGAGGGATTTGGTTATGTTCCTTCAGCATCTTATGTCGATGAAAATGGTGTCTACCAGCCAGTATTTACTCATCAAGTAGCTTACATGAGATGCCGTCGTTGTGGTATCAACGGTGACCACTGGGAGCCATTTTGCCTGAATCCAATCAATGATCCGCCAGAAAGAGAAGAAGAAGCTGCCAAAAAGAAGATCTCGTGGAAGTATATATTGAGGAATTTTAATCCAAAAGAATACATTGACAAACATAAGACTCAAGGGAAACTGCAACCATCAAGCAGGAAGGCAGGGATATCAAAATCTCCTGTCTCTCGAAAATGTTTCCATTGCCGAAACCAAACCCCTGCCAAGACAGAGAAGCCTCCTTTACCAGAGGGATTTGGTTATGTTCCTTCGGCATCTTATGTCGATGAAAATGGTGTCTACCAGCCAGTATTTACTCATCGAGTTGTTTACATGAGATGTCATCGTTGTGGTATCAACGGTGACCACTGGGAGCCATTTTGCCTGAATCCAATCAATGATCCGCCAGAAACAGAAGAAGAAGCTGCCAAAAAGAAGATCTCGTGGAAGTATATATTGAGGAATTTTAATCCAAAAGAATATCTTGAAAAACATAAGACTCAAGAACCGGAATTCGACTCACCACGATTGCCGGTATTGAAATTTACTATTCCCAAGTCTCGAAGAGCTGTTTTCCGCCTTGTGTTTGGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

272

Amino Acids

31.52

Weight (kDa)

9.47

Isoelectric Point (pI)

60.05

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 3 cut(s) 61, 153, 522
AciI CCGC 3 cut(s) 257, 626, 799
AclWI GGATC 2 cut(s) 248, 617
AcsI RAATTY 4 cut(s) 316, 685, 734, 764
AcuI CTGAAG 1 cut(s) 108
AcyI GRCGYC 1 cut(s) 70
AfiI CCNNNNNNNGG 1 cut(s) 84
AgsI TTSAA 2 cut(s) 710, 763
AluBI AGCT 4 cut(s) 182, 278, 647, 791
AluI AGCT 4 cut(s) 182, 278, 647, 791
Alw26I GTCTC 2 cut(s) 412, 786
AlwI GGATC 2 cut(s) 248, 617
ApeKI GCWGC 2 cut(s) 278, 647
ApoI RAATTY 4 cut(s) 316, 685, 734, 764
Asp700I GAANNNNTTC 1 cut(s) 43
AsuHPI GGTGA 3 cut(s) 227, 596, 735
BauI CACGAG 2 cut(s) 295, 664
BbvI GCAGC 2 cut(s) 265, 634
BccI CCATC 1 cut(s) 379
BceAI ACGGC 1 cut(s) 180
BcoDI GTCTC 2 cut(s) 412, 786
BfaI CTAG 1 cut(s) 84
BglII AGATCT 2 cut(s) 291, 660
BisI GCNGC 2 cut(s) 279, 648
BlsI GCNGC 2 cut(s) 280, 649
BmiI GGNNCC 2 cut(s) 228, 597
BmrI ACTGGG 2 cut(s) 232, 601
BmsI GCATC 3 cut(s) 137, 182, 506
BmuI ACTGGG 2 cut(s) 232, 601
BpuEI CTTGAG 2 cut(s) 339, 708
BsaHI GRCGYC 1 cut(s) 70
BsaWI WCCGGW 1 cut(s) 730
Bsc4I CCNNNNNNNGG 1 cut(s) 84
Bse118I RCCGGY 1 cut(s) 754
Bse1I ACTGG 4 cut(s) 161, 227, 530, 596
Bse3DI GCAATG 1 cut(s) 424
BseLI CCNNNNNNNGG 1 cut(s) 84
BseMI GCAATG 1 cut(s) 424
BseNI ACTGG 4 cut(s) 161, 227, 530, 596
BseXI GCAGC 2 cut(s) 265, 634
BsiSI CCGG 2 cut(s) 731, 755
BslI CCNNNNNNNGG 1 cut(s) 84
BsmAI GTCTC 2 cut(s) 412, 786
Bsp143I GATC 4 cut(s) 253, 291, 622, 660
BspACI CCGC 3 cut(s) 257, 626, 799
BspLI GGNNCC 2 cut(s) 228, 597
BspPI GGATC 2 cut(s) 248, 617
BspQI GCTCTTC 1 cut(s) 781
BsrDI GCAATG 1 cut(s) 424
BsrFI RCCGGY 1 cut(s) 754
BsrI ACTGG 4 cut(s) 161, 227, 530, 596
BssAI RCCGGY 1 cut(s) 754
BssMI GATC 4 cut(s) 253, 291, 622, 660
BssNI GRCGYC 1 cut(s) 70
BssSI CACGAG 2 cut(s) 295, 664
Bst2BI CACGAG 2 cut(s) 295, 664
Bst4CI ACNGT 2 cut(s) 215, 584
Bst6I CTCTTC 1 cut(s) 781
BstACI GRCGYC 1 cut(s) 70
BstEII GGTNACC 2 cut(s) 215, 584
BstKTI GATC 4 cut(s) 256, 294, 625, 663
BstMAI GTCTC 2 cut(s) 412, 786
BstMBI GATC 4 cut(s) 253, 291, 622, 660
BstPI GGTNACC 2 cut(s) 215, 584
BstV1I GCAGC 2 cut(s) 265, 634
BstX2I RGATCY 2 cut(s) 291, 660
BstYI RGATCY 2 cut(s) 291, 660
BtsIMutI CAGTG 2 cut(s) 220, 589
Cfr10I RCCGGY 1 cut(s) 754
CseI GACGC 1 cut(s) 78
CviAII CATG 2 cut(s) 187, 556
CviJI RGCY 9 cut(s) 160, 182, 229, 278, 460, 529, 598, 647, 791
CviKI_1 RGCY 9 cut(s) 160, 182, 229, 278, 460, 529, 598, 647, 791
DpnI GATC 4 cut(s) 255, 293, 624, 662
DpnII GATC 4 cut(s) 253, 291, 622, 660
Eam1104I CTCTTC 1 cut(s) 781
EarI CTCTTC 1 cut(s) 781
EciI GGCGGA 3 cut(s) 246, 615, 788
Eco32I GATATC 1 cut(s) 393
Eco57I CTGAAG 1 cut(s) 108
Eco91I GGTNACC 2 cut(s) 215, 584
EcoO65I GGTNACC 2 cut(s) 215, 584
EcoRI GAATTC 1 cut(s) 734
EcoRV GATATC 1 cut(s) 393
FaeI CATG 2 cut(s) 190, 559
FalI AAGNNNNNCTT 2 cut(s) 690, 722
FatI CATG 2 cut(s) 186, 555
FblI GTMKAC 3 cut(s) 61, 153, 522
Fnu4HI GCNGC 2 cut(s) 279, 648
Fsp4HI GCNGC 2 cut(s) 279, 648
FspBI CTAG 1 cut(s) 84
GluI GCNGC 2 cut(s) 279, 648
HapII CCGG 2 cut(s) 731, 755
HgaI GACGC 1 cut(s) 78
Hin1I GRCGYC 1 cut(s) 70
Hin1II CATG 2 cut(s) 190, 559
HincII GTYRAC 1 cut(s) 62
HindII GTYRAC 1 cut(s) 62
HinfI GANTC 6 cut(s) 20, 241, 352, 610, 721, 740
HpaII CCGG 2 cut(s) 731, 755
HphI GGTGA 3 cut(s) 227, 596, 735
Hpy166II GTNNAC 4 cut(s) 62, 154, 523, 553
Hpy188III TCNNGA 5 cut(s) 24, 411, 707, 725, 783
Hpy8I GTNNAC 4 cut(s) 62, 154, 523, 553
Hpy99I CGWCG 2 cut(s) 72, 201
HpyAV CCTTC 4 cut(s) 8, 132, 376, 501
HpyCH4III ACNGT 2 cut(s) 215, 584
HpyCH4V TGCA 2 cut(s) 54, 367
Hsp92I GRCGYC 1 cut(s) 70
Hsp92II CATG 2 cut(s) 190, 559
Kzo9I GATC 4 cut(s) 253, 291, 622, 660
LguI GCTCTTC 1 cut(s) 781
LmnI GCTCC 2 cut(s) 226, 595
Lsp1109I GCAGC 2 cut(s) 265, 634
LweI GCATC 3 cut(s) 137, 182, 506
MaeI CTAG 1 cut(s) 84
MaeIII GTNAC 2 cut(s) 215, 584
MalI GATC 4 cut(s) 255, 293, 624, 662
MboI GATC 4 cut(s) 253, 291, 622, 660
MboII GAAGA 7 cut(s) 281, 284, 301, 650, 653, 670, 798
MflI RGATCY 2 cut(s) 291, 660
MluCI AATT 4 cut(s) 316, 685, 734, 764
MlyI GAGTC 3 cut(s) 346, 715, 734
MnlI CCTC 5 cut(s) 97, 306, 466, 471, 675
MroXI GAANNNNTTC 1 cut(s) 43
MseI TTAA 2 cut(s) 321, 690
MslI CAYNNNNRTG 1 cut(s) 570
MspI CCGG 2 cut(s) 731, 755
NdeII GATC 4 cut(s) 253, 291, 622, 660
NlaIII CATG 2 cut(s) 190, 559
NlaIV GGNNCC 2 cut(s) 228, 597
NmuCI GTSAC 2 cut(s) 215, 584
PciSI GCTCTTC 1 cut(s) 781
PdmI GAANNNNTTC 1 cut(s) 43
PfeI GAWTC 3 cut(s) 20, 241, 610
PkrI GCNGC 2 cut(s) 280, 649
PleI GAGTC 3 cut(s) 346, 715, 734
PpsI GAGTC 3 cut(s) 346, 715, 734
PspEI GGTNACC 2 cut(s) 215, 584
PspN4I GGNNCC 2 cut(s) 228, 597
PsuI RGATCY 2 cut(s) 291, 660
RseI CAYNNNNRTG 1 cut(s) 570
SalI GTCGAC 1 cut(s) 60
SapI GCTCTTC 1 cut(s) 781
SaqAI TTAA 2 cut(s) 321, 690
SatI GCNGC 2 cut(s) 279, 648
Sau3AI GATC 4 cut(s) 253, 291, 622, 660
SchI GAGTC 3 cut(s) 346, 715, 734
SetI ASST 5 cut(s) 59, 184, 280, 649, 793
SfaNI GCATC 3 cut(s) 137, 182, 506
SmiMI CAYNNNNRTG 1 cut(s) 570
SmlI CTYRAG 2 cut(s) 354, 723
SmoI CTYRAG 2 cut(s) 354, 723
Sse9I AATT 4 cut(s) 316, 685, 734, 764
SsiI CCGC 3 cut(s) 257, 626, 799
SspMI CTAG 1 cut(s) 84
TaaI ACNGT 2 cut(s) 215, 584
TaqI TCGA 7 cut(s) 61, 138, 412, 507, 544, 738, 784
TasI AATT 4 cut(s) 316, 685, 734, 764
TfiI GAWTC 3 cut(s) 20, 241, 610
Tru1I TTAA 2 cut(s) 321, 690
Tru9I TTAA 2 cut(s) 321, 690
TscAI CASTG 2 cut(s) 227, 596
TseFI GTSAC 2 cut(s) 215, 584
TseI GCWGC 2 cut(s) 278, 647
Tsp45I GTSAC 2 cut(s) 215, 584
TspDTI ATGAA 3 cut(s) 27, 156, 525
TspRI CASTG 2 cut(s) 227, 596
XapI RAATTY 4 cut(s) 316, 685, 734, 764
XmiI GTMKAC 3 cut(s) 61, 153, 522
XmnI GAANNNNTTC 1 cut(s) 43
XspI CTAG 1 cut(s) 84
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.