Rorug02G0187300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0005517
Physical Location & Seq
Reverse (-)
17474807 .. 17475729
923 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0187300.1

Sequence Viewer

Length: 561 bp
ATGTCTACCGGAATTGTGACCCGGTTCAAGGAAGTGGGCCAATCGACGATGGCGGCGCACCAGCTGTGGTCCGAATTCACCGACTCGACCGCCCTCAGCCTCCCTTCAAGCCTCTTCGACGCCTCGACCCGAGTCTTCCAGAACTTGACCCGGTGGGAGCCGGCGCCACCGGTGACGACGATCCTGAGCCTGCGCCTTGCCGGCGTTGTTGCGAAGGTCGGGGGAGAAGGAGCCTCCAAGGTTGCGTCGGTTTTCTGGAAGGAGAATGAGCTAAACATGGTTGATACGCCGGGACATGCAGACTTTGGTGGTGAAGTTGAGCGGGTTGTTGGTATGGTTGAAGGGGCAGTTTTAGTTGTTGATGCCGAGGAAGATCTGCTAGCACAGACCAAGTTTGTGCTTGCCAAAGCCTTAAAATACGGGCTGCGTCCTATTCTTCTTCTAAACAAAGTAGATCGGCCTGGCAGGTACTTATTCTCGTCTTTAATTTGCAAAATGTGCAAATTAAACAATTGCATGAATAATATGATAGTGGAAAGACTAATTAAGGAAAACATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

186

Amino Acids

20.44

Weight (kDa)

6.74

Isoelectric Point (pI)

31.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GTP_EFTU PF00009 79 - 155 7.1e-23 Elongation factor Tu GTP binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 456
AccB1I GGYRCC 1 cut(s) 163
AccBSI CCGCTC 1 cut(s) 322
AccI GTMKAC 1 cut(s) 5
AciI CCGC 3 cut(s) 53, 90, 322
AclWI GGATC 1 cut(s) 175
AcsI RAATTY 1 cut(s) 74
AcyI GRCGYC 2 cut(s) 120, 164
AfaI GTAC 1 cut(s) 470
AfiI CCNNNNNNNGG 1 cut(s) 28
AflIII ACRYGT 1 cut(s) 555
AgeI ACCGGT 1 cut(s) 169
AgsI TTSAA 3 cut(s) 28, 108, 341
AjnI CCWGG 1 cut(s) 460
AluBI AGCT 2 cut(s) 64, 271
AluI AGCT 2 cut(s) 64, 271
AlwI GGATC 1 cut(s) 175
Ama87I CYCGRG 1 cut(s) 129
AoxI GGCC 2 cut(s) 37, 458
ApeKI GCWGC 1 cut(s) 424
ApoI RAATTY 1 cut(s) 74
AsiGI ACCGGT 1 cut(s) 169
AspLEI GCGC 3 cut(s) 58, 166, 195
AspS9I GGNCC 2 cut(s) 37, 69
AsuC2I CCSGG 3 cut(s) 22, 151, 291
AsuHPI GGTGA 3 cut(s) 70, 184, 323
AsuNHI GCTAGC 1 cut(s) 379
AvaI CYCGRG 1 cut(s) 129
AvaII GGWCC 1 cut(s) 69
BanI GGYRCC 1 cut(s) 163
BbsI GAAGAC 1 cut(s) 127
BbvCI CCTCAGC 1 cut(s) 95
BbvI GCAGC 1 cut(s) 411
BccI CCATC 1 cut(s) 43
BciT130I CCWGG 1 cut(s) 462
BcnI CCSGG 3 cut(s) 22, 151, 291
BfaI CTAG 1 cut(s) 380
BfoI RGCGCY 1 cut(s) 167
BfuAI ACCTGC 1 cut(s) 456
BglI GCCNNNNNGGC 1 cut(s) 201
BglII AGATCT 1 cut(s) 373
BisI GCNGC 2 cut(s) 54, 425
BlsI GCNGC 2 cut(s) 55, 426
Bme1390I CCNGG 4 cut(s) 22, 151, 291, 462
Bme18I GGWCC 1 cut(s) 69
BmeT110I CYCGRG 1 cut(s) 129
BmgT120I GGNCC 2 cut(s) 37, 69
BmiI GGNNCC 3 cut(s) 159, 165, 232
BmrFI CCNGG 4 cut(s) 22, 151, 291, 462
BmsI GCATC 1 cut(s) 352
BmtI GCTAGC 1 cut(s) 383
BoxI GACNNNNGTC 1 cut(s) 131
BpiI GAAGAC 1 cut(s) 127
Bpu10I CCTNAGC 2 cut(s) 95, 185
BpuMI CCSGG 3 cut(s) 22, 151, 291
BsaHI GRCGYC 2 cut(s) 120, 164
BsaJI CCNNGG 2 cut(s) 237, 366
BsaWI WCCGGW 2 cut(s) 8, 169
Bsc4I CCNNNNNNNGG 1 cut(s) 28
Bse118I RCCGGY 3 cut(s) 160, 169, 200
BseBI CCWGG 1 cut(s) 462
BseDI CCNNGG 2 cut(s) 237, 366
BseLI CCNNNNNNNGG 1 cut(s) 28
BseMII CTCAG 2 cut(s) 109, 176
BseXI GCAGC 1 cut(s) 411
Bsh1285I CGRYCG 1 cut(s) 90
BshFI GGCC 2 cut(s) 39, 460
BshNI GGYRCC 1 cut(s) 163
BshTI ACCGGT 1 cut(s) 169
BsiEI CGRYCG 1 cut(s) 90
BsiHKCI CYCGRG 1 cut(s) 129
BsiSI CCGG 7 cut(s) 9, 22, 151, 161, 170, 201, 290
BslFI GGGAC 1 cut(s) 306
BslI CCNNNNNNNGG 1 cut(s) 28
BsmFI GGGAC 1 cut(s) 306
BsnI GGCC 2 cut(s) 39, 460
BsoBI CYCGRG 1 cut(s) 129
Bsp143I GATC 3 cut(s) 180, 373, 454
BspACI CCGC 3 cut(s) 53, 90, 322
BspANI GGCC 2 cut(s) 39, 460
BspCNI CTCAG 2 cut(s) 108, 177
BspLI GGNNCC 3 cut(s) 159, 165, 232
BspMI ACCTGC 1 cut(s) 456
BspOI GCTAGC 1 cut(s) 383
BspPI GGATC 1 cut(s) 175
BspT107I GGYRCC 1 cut(s) 163
BsrBI CCGCTC 1 cut(s) 322
BsrFI RCCGGY 3 cut(s) 160, 169, 200
BssAI RCCGGY 3 cut(s) 160, 169, 200
BssECI CCNNGG 2 cut(s) 237, 366
BssMI GATC 3 cut(s) 180, 373, 454
BssNI GRCGYC 2 cut(s) 120, 164
BssT1I CCWWGG 1 cut(s) 237
Bst2UI CCWGG 1 cut(s) 462
Bst6I CTCTTC 1 cut(s) 119
BstACI GRCGYC 2 cut(s) 120, 164
BstAPI GCANNNNNTGC 1 cut(s) 498
BstC8I GCNNGC 5 cut(s) 162, 191, 202, 381, 402
BstDEI CTNAG 2 cut(s) 95, 185
BstH2I RGCGCY 1 cut(s) 167
BstHHI GCGC 3 cut(s) 58, 166, 195
BstKTI GATC 3 cut(s) 183, 376, 457
BstMBI GATC 3 cut(s) 180, 373, 454
BstMCI CGRYCG 1 cut(s) 90
BstMWI GCNNNNNNNGC 2 cut(s) 201, 498
BstNI CCWGG 1 cut(s) 462
BstNSI RCATGY 2 cut(s) 299, 559
BstPAI GACNNNNGTC 1 cut(s) 131
BstSCI CCNGG 4 cut(s) 20, 149, 289, 460
BstV1I GCAGC 1 cut(s) 411
BstV2I GAAGAC 1 cut(s) 127
BstX2I RGATCY 1 cut(s) 373
BstYI RGATCY 1 cut(s) 373
BsuRI GGCC 2 cut(s) 39, 460
BveI ACCTGC 1 cut(s) 456
Cac8I GCNNGC 5 cut(s) 162, 191, 202, 381, 402
CfoI GCGC 3 cut(s) 58, 166, 195
Cfr10I RCCGGY 3 cut(s) 160, 169, 200
Cfr13I GGNCC 2 cut(s) 37, 69
CseI GACGC 3 cut(s) 128, 234, 416
Csp6I GTAC 1 cut(s) 469
CspAI ACCGGT 1 cut(s) 169
CviAII CATG 4 cut(s) 277, 296, 517, 556
CviQI GTAC 1 cut(s) 469
DdeI CTNAG 2 cut(s) 95, 185
DinI GGCGCC 1 cut(s) 165
DpnI GATC 3 cut(s) 182, 375, 456
DpnII GATC 3 cut(s) 180, 373, 454
Eam1104I CTCTTC 1 cut(s) 119
EarI CTCTTC 1 cut(s) 119
Eco130I CCWWGG 1 cut(s) 237
Eco47I GGWCC 1 cut(s) 69
Eco88I CYCGRG 1 cut(s) 129
EcoRI GAATTC 1 cut(s) 74
EcoRII CCWGG 1 cut(s) 460
EcoT14I CCWWGG 1 cut(s) 237
EgeI GGCGCC 1 cut(s) 165
EheI GGCGCC 1 cut(s) 165
ErhI CCWWGG 1 cut(s) 237
FaeI CATG 4 cut(s) 280, 299, 520, 559
FaiI YATR 6 cut(s) 278, 297, 335, 518, 527, 557
FaqI GGGAC 1 cut(s) 306
FatI CATG 4 cut(s) 276, 295, 516, 555
FauI CCCGC 1 cut(s) 315
FblI GTMKAC 1 cut(s) 5
Fnu4HI GCNGC 2 cut(s) 54, 425
Fsp4HI GCNGC 2 cut(s) 54, 425
FspBI CTAG 1 cut(s) 380
GlaI GCGC 3 cut(s) 57, 165, 194
GluI GCNGC 2 cut(s) 54, 425
HaeII RGCGCY 1 cut(s) 167
HaeIII GGCC 2 cut(s) 39, 460
HapII CCGG 7 cut(s) 9, 22, 151, 161, 170, 201, 290
HgaI GACGC 3 cut(s) 128, 234, 416
HhaI GCGC 3 cut(s) 58, 166, 195
Hin1I GRCGYC 2 cut(s) 120, 164
Hin1II CATG 4 cut(s) 280, 299, 520, 559
Hin6I GCGC 3 cut(s) 56, 164, 193
HinP1I GCGC 3 cut(s) 56, 164, 193
HinfI GANTC 2 cut(s) 83, 132
HpaII CCGG 7 cut(s) 9, 22, 151, 161, 170, 201, 290
HphI GGTGA 3 cut(s) 70, 184, 323
Hpy166II GTNNAC 1 cut(s) 6
Hpy188I TCNGA 1 cut(s) 73
Hpy188III TCNNGA 3 cut(s) 139, 184, 256
Hpy8I GTNNAC 1 cut(s) 6
Hpy99I CGWCG 4 cut(s) 49, 122, 181, 250
HpyAV CCTTC 5 cut(s) 114, 208, 221, 253, 335
HpyCH4V TGCA 4 cut(s) 299, 492, 501, 516
HpyF10VI GCNNNNNNNGC 2 cut(s) 201, 498
HpyF3I CTNAG 2 cut(s) 95, 185
Hsp92I GRCGYC 2 cut(s) 120, 164
Hsp92II CATG 4 cut(s) 280, 299, 520, 559
HspAI GCGC 3 cut(s) 56, 164, 193
KasI GGCGCC 1 cut(s) 163
KroI GCCGGC 2 cut(s) 160, 200
KroNI GCCGGC 2 cut(s) 162, 202
Kzo9I GATC 3 cut(s) 180, 373, 454
LmnI GCTCC 2 cut(s) 157, 230
Lsp1109I GCAGC 1 cut(s) 411
LweI GCATC 1 cut(s) 352
MaeI CTAG 1 cut(s) 380
MaeIII GTNAC 2 cut(s) 16, 172
MalI GATC 3 cut(s) 182, 375, 456
MbiI CCGCTC 1 cut(s) 322
MboI GATC 3 cut(s) 180, 373, 454
MboII GAAGA 5 cut(s) 106, 127, 383, 428, 431
MfeI CAATTG 1 cut(s) 511
MflI RGATCY 1 cut(s) 373
MluCI AATT 6 cut(s) 12, 74, 486, 503, 511, 543
Mly113I GGCGCC 1 cut(s) 164
MlyI GAGTC 2 cut(s) 77, 141
MnlI CCTC 6 cut(s) 104, 110, 122, 133, 244, 361
MroNI GCCGGC 2 cut(s) 160, 200
MseI TTAA 4 cut(s) 413, 485, 506, 546
MspA1I CMGCKG 1 cut(s) 64
MspI CCGG 7 cut(s) 9, 22, 151, 161, 170, 201, 290
MspR9I CCNGG 4 cut(s) 22, 151, 291, 462
MunI CAATTG 1 cut(s) 511
MvaI CCWGG 1 cut(s) 462
MwoI GCNNNNNNNGC 2 cut(s) 201, 498
NaeI GCCGGC 2 cut(s) 162, 202
NarI GGCGCC 1 cut(s) 164
NciI CCSGG 3 cut(s) 22, 151, 291
NdeII GATC 3 cut(s) 180, 373, 454
NgoMIV GCCGGC 2 cut(s) 160, 200
NheI GCTAGC 1 cut(s) 379
NlaIII CATG 4 cut(s) 280, 299, 520, 559
NlaIV GGNNCC 3 cut(s) 159, 165, 232
NmeAIII GCCGAG 1 cut(s) 391
NmuCI GTSAC 2 cut(s) 16, 172
NspI RCATGY 2 cut(s) 299, 559
PciI ACATGT 1 cut(s) 555
PdiI GCCGGC 2 cut(s) 162, 202
PinAI ACCGGT 1 cut(s) 169
PkrI GCNGC 2 cut(s) 55, 426
PleI GAGTC 2 cut(s) 77, 140
PluTI GGCGCC 1 cut(s) 167
PpsI GAGTC 2 cut(s) 77, 140
PscI ACATGT 1 cut(s) 555
PshAI GACNNNNGTC 1 cut(s) 131
Psp6I CCWGG 1 cut(s) 460
PspGI CCWGG 1 cut(s) 460
PspN4I GGNNCC 3 cut(s) 159, 165, 232
PspPI GGNCC 2 cut(s) 37, 69
PsuI RGATCY 1 cut(s) 373
PvuII CAGCTG 1 cut(s) 64
RsaI GTAC 1 cut(s) 470
RsaNI GTAC 1 cut(s) 469
SaqAI TTAA 4 cut(s) 413, 485, 506, 546
SatI GCNGC 2 cut(s) 54, 425
Sau3AI GATC 3 cut(s) 180, 373, 454
Sau96I GGNCC 2 cut(s) 37, 69
SchI GAGTC 2 cut(s) 77, 141
ScrFI CCNGG 4 cut(s) 22, 151, 291, 462
SetI ASST 5 cut(s) 66, 219, 243, 273, 470
SfaNI GCATC 1 cut(s) 352
SfoI GGCGCC 1 cut(s) 165
SgrAI CRCCGGYG 1 cut(s) 169
SinI GGWCC 1 cut(s) 69
Sse9I AATT 6 cut(s) 12, 74, 486, 503, 511, 543
SsiI CCGC 3 cut(s) 53, 90, 322
SspDI GGCGCC 1 cut(s) 163
SspMI CTAG 1 cut(s) 380
StyD4I CCNGG 4 cut(s) 20, 149, 289, 460
StyI CCWWGG 1 cut(s) 237
TaqI TCGA 4 cut(s) 44, 86, 117, 125
TasI AATT 6 cut(s) 12, 74, 486, 503, 511, 543
TauI GCSGC 1 cut(s) 56
Tru1I TTAA 4 cut(s) 413, 485, 506, 546
Tru9I TTAA 4 cut(s) 413, 485, 506, 546
TseFI GTSAC 2 cut(s) 16, 172
TseI GCWGC 1 cut(s) 424
Tsp45I GTSAC 2 cut(s) 16, 172
TspDTI ATGAA 1 cut(s) 533
VpaK11BI GGWCC 1 cut(s) 69
XapI RAATTY 1 cut(s) 74
XceI RCATGY 2 cut(s) 299, 559
XmiI GTMKAC 1 cut(s) 5
XspI CTAG 1 cut(s) 380
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.