Rh2DG249300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0005517
Physical Location & Seq
Reverse (-)
25649706 .. 25651057
1352 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG249300.1

Sequence Viewer

Length: 489 bp
ATGGCAGAAATCGAAGCCACCATCGCCCGCGGCGAACAGACAGAGATTGATAAAGGGGAATCGCAAACACCATGTACAAAGTCACGAAAACGAAAACGAAATGCTCATTGTTGTTGCACGTGTCATGAACCCAAAACCCGACGCCGTGCCAAAACTAGGAAACCTCCTTTACCAGAGGGATTTGGTTATGTTCCTTCGGCAATATGTGTGGATAAGAATGGTAACTACATTCCAAGATTTTCTGATCGTGTTGCTTACATGTCTTGTCACCGTTGTGGTATGAAGGGTGACCACTGGGATCCATTTTGCCCCAACTCGATCAATGATCCGCCGGACTCAGGAGAAGCTGCTCCTACCCGTAACCCGTCATGGAATTATATATTGAGGAATTTCAACCTGAAAGAATATCTTGAGAAAAAGAATAAGACTGATGGGAAACCAGAACCACCACGGAGGAAGGCGGTGAAATCAAAAGTGTCCAATCCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

162

Amino Acids

18.44

Weight (kDa)

9.49

Isoelectric Point (pI)

61.78

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 30
AciI CCGC 4 cut(s) 28, 30, 329, 461
AclWI GGATC 3 cut(s) 293, 306, 320
AcsI RAATTY 1 cut(s) 388
AcvI CACGTG 1 cut(s) 120
AcyI GRCGYC 1 cut(s) 142
AfaI GTAC 1 cut(s) 76
AfiI CCNNNNNNNGG 2 cut(s) 156, 338
AflIII ACRYGT 2 cut(s) 119, 258
AgsI TTSAA 1 cut(s) 394
AleI CACNNNNGTG 1 cut(s) 273
AluBI AGCT 1 cut(s) 347
AluI AGCT 1 cut(s) 347
AlwI GGATC 3 cut(s) 293, 306, 320
ApeKI GCWGC 1 cut(s) 347
ApoI RAATTY 1 cut(s) 388
AsuHPI GGTGA 3 cut(s) 260, 299, 475
BamHI GGATCC 1 cut(s) 298
BbrPI CACGTG 1 cut(s) 120
BbvI GCAGC 1 cut(s) 334
BccI CCATC 2 cut(s) 29, 425
BceAI ACGGC 1 cut(s) 129
BfaI CTAG 1 cut(s) 156
BisI GCNGC 2 cut(s) 31, 348
BlsI GCNGC 2 cut(s) 32, 349
BmiI GGNNCC 1 cut(s) 300
BmrI ACTGGG 1 cut(s) 304
BmuI ACTGGG 1 cut(s) 304
BpuEI CTTGAG 1 cut(s) 431
BsaAI YACGTR 1 cut(s) 120
BsaHI GRCGYC 1 cut(s) 142
BsaJI CCNNGG 2 cut(s) 28, 449
Bsc4I CCNNNNNNNGG 2 cut(s) 156, 338
Bse1I ACTGG 1 cut(s) 299
BseDI CCNNGG 2 cut(s) 28, 449
BseLI CCNNNNNNNGG 2 cut(s) 156, 338
BseMII CTCAG 1 cut(s) 351
BseNI ACTGG 1 cut(s) 299
BseXI GCAGC 1 cut(s) 334
Bsh1236I CGCG 1 cut(s) 30
BsiSI CCGG 1 cut(s) 332
BslI CCNNNNNNNGG 2 cut(s) 156, 338
Bsp1407I TGTACA 1 cut(s) 74
Bsp143I GATC 4 cut(s) 244, 298, 318, 325
BspACI CCGC 4 cut(s) 28, 30, 329, 461
BspCNI CTCAG 1 cut(s) 350
BspFNI CGCG 1 cut(s) 30
BspHI TCATGA 1 cut(s) 124
BspLI GGNNCC 1 cut(s) 300
BspPI GGATC 3 cut(s) 293, 306, 320
BsrGI TGTACA 1 cut(s) 74
BsrI ACTGG 1 cut(s) 299
BssECI CCNNGG 2 cut(s) 28, 449
BssMI GATC 4 cut(s) 244, 298, 318, 325
BssNI GRCGYC 1 cut(s) 142
Bst4CI ACNGT 1 cut(s) 272
BstACI GRCGYC 1 cut(s) 142
BstAUI TGTACA 1 cut(s) 74
BstBAI YACGTR 1 cut(s) 120
BstC8I GCNNGC 1 cut(s) 28
BstDEI CTNAG 1 cut(s) 337
BstDSI CCRYGG 2 cut(s) 28, 449
BstEII GGTNACC 1 cut(s) 287
BstFNI CGCG 1 cut(s) 30
BstKTI GATC 4 cut(s) 247, 301, 321, 328
BstMBI GATC 4 cut(s) 244, 298, 318, 325
BstMWI GCNNNNNNNGC 1 cut(s) 23
BstNSI RCATGY 1 cut(s) 262
BstPI GGTNACC 1 cut(s) 287
BstUI CGCG 1 cut(s) 30
BstV1I GCAGC 1 cut(s) 334
BstX2I RGATCY 1 cut(s) 298
BstYI RGATCY 1 cut(s) 298
BtgI CCRYGG 2 cut(s) 28, 449
BtgZI GCGATG 1 cut(s) 7
BtsIMutI CAGTG 1 cut(s) 292
Cac8I GCNNGC 1 cut(s) 28
CciI TCATGA 1 cut(s) 124
Cfr42I CCGCGG 1 cut(s) 31
CseI GACGC 1 cut(s) 150
Csp6I GTAC 1 cut(s) 75
CspCI CAANNNNNGTGG 2 cut(s) 189, 224
CviAII CATG 4 cut(s) 72, 125, 259, 369
CviJI RGCY 2 cut(s) 17, 347
CviKI_1 RGCY 2 cut(s) 17, 347
CviQI GTAC 1 cut(s) 75
DdeI CTNAG 1 cut(s) 337
DpnI GATC 4 cut(s) 246, 300, 320, 327
DpnII GATC 4 cut(s) 244, 298, 318, 325
EciI GGCGGA 1 cut(s) 318
Eco72I CACGTG 1 cut(s) 120
Eco91I GGTNACC 1 cut(s) 287
EcoO65I GGTNACC 1 cut(s) 287
FaeI CATG 4 cut(s) 75, 128, 262, 372
FalI AAGNNNNNCTT 2 cut(s) 393, 425
FatI CATG 4 cut(s) 71, 124, 258, 368
FauI CCCGC 1 cut(s) 35
Fnu4HI GCNGC 2 cut(s) 31, 348
Fsp4HI GCNGC 2 cut(s) 31, 348
FspBI CTAG 1 cut(s) 156
GluI GCNGC 2 cut(s) 31, 348
HapII CCGG 1 cut(s) 332
HgaI GACGC 1 cut(s) 150
Hin1I GRCGYC 1 cut(s) 142
Hin1II CATG 4 cut(s) 75, 128, 262, 372
HinfI GANTC 2 cut(s) 59, 335
HpaII CCGG 1 cut(s) 332
HphI GGTGA 3 cut(s) 260, 299, 475
Hpy188I TCNGA 1 cut(s) 244
Hpy188III TCNNGA 4 cut(s) 84, 125, 339, 410
Hpy99I CGWCG 1 cut(s) 144
HpyAV CCTTC 3 cut(s) 204, 277, 451
HpyCH4III ACNGT 1 cut(s) 272
HpyCH4IV ACGT 1 cut(s) 119
HpyCH4V TGCA 1 cut(s) 117
HpyF10VI GCNNNNNNNGC 1 cut(s) 23
HpyF3I CTNAG 1 cut(s) 337
HpySE526I ACGT 1 cut(s) 119
Hsp92I GRCGYC 1 cut(s) 142
Hsp92II CATG 4 cut(s) 75, 128, 262, 372
KspI CCGCGG 1 cut(s) 31
Kzo9I GATC 4 cut(s) 244, 298, 318, 325
LmnI GCTCC 1 cut(s) 355
LpnPI CCDG 6 cut(s) 186, 280, 324, 345, 410, 453
Lsp1109I GCAGC 1 cut(s) 334
MaeI CTAG 1 cut(s) 156
MaeII ACGT 1 cut(s) 119
MaeIII GTNAC 5 cut(s) 81, 221, 266, 287, 359
MalI GATC 4 cut(s) 246, 300, 320, 327
MboI GATC 4 cut(s) 244, 298, 318, 325
MflI RGATCY 1 cut(s) 298
MluCI AATT 2 cut(s) 373, 388
MlyI GAGTC 1 cut(s) 329
MnlI CCTC 4 cut(s) 169, 174, 378, 447
MslI CAYNNNNRTG 1 cut(s) 273
MspA1I CMGCKG 1 cut(s) 30
MspI CCGG 1 cut(s) 332
MvnI CGCG 1 cut(s) 30
MwoI GCNNNNNNNGC 1 cut(s) 23
NdeII GATC 4 cut(s) 244, 298, 318, 325
NlaIII CATG 4 cut(s) 75, 128, 262, 372
NlaIV GGNNCC 1 cut(s) 300
NmuCI GTSAC 3 cut(s) 81, 266, 287
NspI RCATGY 1 cut(s) 262
OliI CACNNNNGTG 1 cut(s) 273
PagI TCATGA 1 cut(s) 124
PciI ACATGT 1 cut(s) 258
PcsI WCGNNNNNNNCGW 1 cut(s) 30
PfeI GAWTC 1 cut(s) 59
PkrI GCNGC 2 cut(s) 32, 349
PleI GAGTC 1 cut(s) 329
PmaCI CACGTG 1 cut(s) 120
PmlI CACGTG 1 cut(s) 120
PpsI GAGTC 1 cut(s) 329
Ppu21I YACGTR 1 cut(s) 120
PscI ACATGT 1 cut(s) 258
PspCI CACGTG 1 cut(s) 120
PspEI GGTNACC 1 cut(s) 287
PspN4I GGNNCC 1 cut(s) 300
PsuI RGATCY 1 cut(s) 298
RsaI GTAC 1 cut(s) 76
RsaNI GTAC 1 cut(s) 75
RseI CAYNNNNRTG 1 cut(s) 273
SacII CCGCGG 1 cut(s) 31
SatI GCNGC 2 cut(s) 31, 348
Sau3AI GATC 4 cut(s) 244, 298, 318, 325
SchI GAGTC 1 cut(s) 329
SetI ASST 4 cut(s) 122, 166, 349, 399
Sfr303I CCGCGG 1 cut(s) 31
SgrBI CCGCGG 1 cut(s) 31
SmiMI CAYNNNNRTG 1 cut(s) 273
SmlI CTYRAG 1 cut(s) 410
SmoI CTYRAG 1 cut(s) 410
Sse9I AATT 2 cut(s) 373, 388
SsiI CCGC 4 cut(s) 28, 30, 329, 461
SspMI CTAG 1 cut(s) 156
TaaI ACNGT 1 cut(s) 272
TaiI ACGT 1 cut(s) 122
TaqI TCGA 2 cut(s) 12, 317
TasI AATT 2 cut(s) 373, 388
TatI WGTACW 1 cut(s) 74
TauI GCSGC 1 cut(s) 33
TfiI GAWTC 1 cut(s) 59
TscAI CASTG 1 cut(s) 299
TseFI GTSAC 3 cut(s) 81, 266, 287
TseI GCWGC 1 cut(s) 347
Tsp45I GTSAC 3 cut(s) 81, 266, 287
TspDTI ATGAA 2 cut(s) 141, 296
TspGWI ACGGA 1 cut(s) 466
TspRI CASTG 1 cut(s) 299
XapI RAATTY 1 cut(s) 388
XceI RCATGY 1 cut(s) 262
XspI CTAG 1 cut(s) 156
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.