Rroxscaffold_2G00129300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0005517
Physical Location & Seq
Forward (+)
65109440 .. 65110124
685 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00129300.1

Sequence Viewer

Length: 588 bp
ATGGCAGAAATCGAAGCGGACCGGCGTGTTGAATCCGATGAGGTCGGCACCATCGACCGCGGCAAACAGATGGAGATTGATAAAGATTTCTTATTAATATTATGTTCCGTTACTCATCTTTTGGTTTTCCGATCTCATCAGGGGAATCGCAACCACCATGTACAAAGTCACGATATCGAAAACGAAAACGAAATGCTCATTGTTGTTGCAAGTGTCATGATCCCAAAACCCGACGCCGTGCCAAAACTAGGAAACCTCCTTTACCAGAGGGATTTGGTTATGTTCCTTCGGCAATATGTGTGGATAAGAATGGTAACTACATTCCAAGATTTTCTGATCGTGTTGCTTACATGTCTTGTCAGTTGTCACCGTTGTGGTATGAAGGGTGACCACTGGGATCCATTTTGCCCCAACTCGATCAATGATCCGCCAGACTCAGGAGAAGCTGCTCCTACCCGTAACCCGTCATGGAATTATATATTGAGGAATTTCAATTTGAAAGAATATCTTGACAAAAAGAATAAGACTGATGGGAAACCAGAACCACCACGGAGGAAGGCGGTGAAATCAAAAGTGTCCAATCCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

195

Amino Acids

22.5

Weight (kDa)

7.04

Isoelectric Point (pI)

40.46

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 47
AccII CGCG 1 cut(s) 60
AciI CCGC 5 cut(s) 17, 58, 60, 428, 560
AclWI GGATC 4 cut(s) 214, 392, 405, 419
AcsI RAATTY 1 cut(s) 487
AcyI GRCGYC 1 cut(s) 234
AfaI GTAC 1 cut(s) 162
AfiI CCNNNNNNNGG 2 cut(s) 248, 437
AflIII ACRYGT 1 cut(s) 350
AgsI TTSAA 3 cut(s) 32, 493, 499
AleI CACNNNNGTG 1 cut(s) 372
AluBI AGCT 1 cut(s) 446
AluI AGCT 1 cut(s) 446
AlwI GGATC 4 cut(s) 214, 392, 405, 419
ApeKI GCWGC 1 cut(s) 446
ApoI RAATTY 1 cut(s) 487
AseI ATTAAT 1 cut(s) 95
AspS9I GGNCC 1 cut(s) 19
AsuHPI GGTGA 3 cut(s) 359, 398, 574
AvaII GGWCC 1 cut(s) 19
BamHI GGATCC 1 cut(s) 397
BanI GGYRCC 1 cut(s) 47
BbvI GCAGC 1 cut(s) 433
BccI CCATC 3 cut(s) 59, 64, 524
BceAI ACGGC 1 cut(s) 221
BfaI CTAG 1 cut(s) 248
BisI GCNGC 2 cut(s) 61, 447
BlsI GCNGC 2 cut(s) 62, 448
Bme18I GGWCC 1 cut(s) 19
BmgT120I GGNCC 1 cut(s) 19
BmiI GGNNCC 2 cut(s) 49, 399
BmrI ACTGGG 1 cut(s) 403
BmuI ACTGGG 1 cut(s) 403
BsaHI GRCGYC 1 cut(s) 234
BsaJI CCNNGG 2 cut(s) 58, 548
Bsc4I CCNNNNNNNGG 2 cut(s) 248, 437
Bse118I RCCGGY 1 cut(s) 21
Bse1I ACTGG 1 cut(s) 398
BseDI CCNNGG 2 cut(s) 58, 548
BseLI CCNNNNNNNGG 2 cut(s) 248, 437
BseMII CTCAG 1 cut(s) 450
BseNI ACTGG 1 cut(s) 398
BseXI GCAGC 1 cut(s) 433
Bsh1236I CGCG 1 cut(s) 60
Bsh1285I CGRYCG 1 cut(s) 58
BshNI GGYRCC 1 cut(s) 47
BsiEI CGRYCG 1 cut(s) 58
BsiSI CCGG 1 cut(s) 22
BslI CCNNNNNNNGG 2 cut(s) 248, 437
Bsp1407I TGTACA 1 cut(s) 160
Bsp143I GATC 6 cut(s) 131, 219, 336, 397, 417, 424
BspACI CCGC 5 cut(s) 17, 58, 60, 428, 560
BspCNI CTCAG 1 cut(s) 449
BspFNI CGCG 1 cut(s) 60
BspHI TCATGA 1 cut(s) 216
BspLI GGNNCC 2 cut(s) 49, 399
BspPI GGATC 4 cut(s) 214, 392, 405, 419
BspT107I GGYRCC 1 cut(s) 47
BsrFI RCCGGY 1 cut(s) 21
BsrGI TGTACA 1 cut(s) 160
BsrI ACTGG 1 cut(s) 398
BssAI RCCGGY 1 cut(s) 21
BssECI CCNNGG 2 cut(s) 58, 548
BssMI GATC 6 cut(s) 131, 219, 336, 397, 417, 424
BssNI GRCGYC 1 cut(s) 234
Bst4CI ACNGT 1 cut(s) 371
BstACI GRCGYC 1 cut(s) 234
BstAUI TGTACA 1 cut(s) 160
BstDEI CTNAG 1 cut(s) 436
BstDSI CCRYGG 2 cut(s) 58, 548
BstEII GGTNACC 1 cut(s) 386
BstFNI CGCG 1 cut(s) 60
BstKTI GATC 6 cut(s) 134, 222, 339, 400, 420, 427
BstMBI GATC 6 cut(s) 131, 219, 336, 397, 417, 424
BstMCI CGRYCG 1 cut(s) 58
BstNSI RCATGY 1 cut(s) 354
BstPI GGTNACC 1 cut(s) 386
BstUI CGCG 1 cut(s) 60
BstV1I GCAGC 1 cut(s) 433
BstX2I RGATCY 1 cut(s) 397
BstYI RGATCY 1 cut(s) 397
BtgI CCRYGG 2 cut(s) 58, 548
BtsIMutI CAGTG 1 cut(s) 391
CciI TCATGA 1 cut(s) 216
Cfr10I RCCGGY 1 cut(s) 21
Cfr13I GGNCC 1 cut(s) 19
Cfr42I CCGCGG 1 cut(s) 61
CpoI CGGWCCG 1 cut(s) 19
CseI GACGC 1 cut(s) 242
Csp6I GTAC 1 cut(s) 161
CspCI CAANNNNNGTGG 2 cut(s) 281, 316
CspI CGGWCCG 1 cut(s) 19
CviAII CATG 4 cut(s) 158, 217, 351, 468
CviJI RGCY 1 cut(s) 446
CviKI_1 RGCY 1 cut(s) 446
CviQI GTAC 1 cut(s) 161
DdeI CTNAG 1 cut(s) 436
DpnI GATC 6 cut(s) 133, 221, 338, 399, 419, 426
DpnII GATC 6 cut(s) 131, 219, 336, 397, 417, 424
EciI GGCGGA 1 cut(s) 417
Eco32I GATATC 1 cut(s) 175
Eco47I GGWCC 1 cut(s) 19
Eco91I GGTNACC 1 cut(s) 386
EcoO65I GGTNACC 1 cut(s) 386
EcoRV GATATC 1 cut(s) 175
FaeI CATG 4 cut(s) 161, 220, 354, 471
FalI AAGNNNNNCTT 2 cut(s) 492, 524
FatI CATG 4 cut(s) 157, 216, 350, 467
Fnu4HI GCNGC 2 cut(s) 61, 447
Fsp4HI GCNGC 2 cut(s) 61, 447
FspBI CTAG 1 cut(s) 248
GluI GCNGC 2 cut(s) 61, 447
HapII CCGG 1 cut(s) 22
HgaI GACGC 1 cut(s) 242
Hin1I GRCGYC 1 cut(s) 234
Hin1II CATG 4 cut(s) 161, 220, 354, 471
HinfI GANTC 3 cut(s) 32, 145, 434
HpaII CCGG 1 cut(s) 22
HphI GGTGA 3 cut(s) 359, 398, 574
Hpy188I TCNGA 3 cut(s) 37, 131, 336
Hpy188III TCNNGA 4 cut(s) 170, 217, 438, 509
Hpy99I CGWCG 1 cut(s) 236
HpyAV CCTTC 3 cut(s) 296, 376, 550
HpyCH4III ACNGT 1 cut(s) 371
HpyCH4V TGCA 1 cut(s) 209
HpyF3I CTNAG 1 cut(s) 436
Hsp92I GRCGYC 1 cut(s) 234
Hsp92II CATG 4 cut(s) 161, 220, 354, 471
KspI CCGCGG 1 cut(s) 61
Kzo9I GATC 6 cut(s) 131, 219, 336, 397, 417, 424
LmnI GCTCC 1 cut(s) 454
LpnPI CCDG 7 cut(s) 35, 125, 278, 379, 423, 444, 552
Lsp1109I GCAGC 1 cut(s) 433
MaeI CTAG 1 cut(s) 248
MaeIII GTNAC 6 cut(s) 109, 167, 313, 365, 386, 458
MalI GATC 6 cut(s) 133, 221, 338, 399, 419, 426
MboI GATC 6 cut(s) 131, 219, 336, 397, 417, 424
MflI RGATCY 1 cut(s) 397
MluCI AATT 3 cut(s) 472, 487, 493
MlyI GAGTC 1 cut(s) 428
MnlI CCTC 5 cut(s) 34, 261, 266, 477, 546
MseI TTAA 1 cut(s) 95
MslI CAYNNNNRTG 1 cut(s) 372
MspA1I CMGCKG 1 cut(s) 60
MspI CCGG 1 cut(s) 22
MvnI CGCG 1 cut(s) 60
NdeII GATC 6 cut(s) 131, 219, 336, 397, 417, 424
NlaIII CATG 4 cut(s) 161, 220, 354, 471
NlaIV GGNNCC 2 cut(s) 49, 399
NmuCI GTSAC 3 cut(s) 167, 365, 386
NspI RCATGY 1 cut(s) 354
OliI CACNNNNGTG 1 cut(s) 372
PagI TCATGA 1 cut(s) 216
PciI ACATGT 1 cut(s) 350
PcsI WCGNNNNNNNCGW 1 cut(s) 51
PfeI GAWTC 2 cut(s) 32, 145
PkrI GCNGC 2 cut(s) 62, 448
PleI GAGTC 1 cut(s) 428
PpsI GAGTC 1 cut(s) 428
PscI ACATGT 1 cut(s) 350
PshBI ATTAAT 1 cut(s) 95
PspEI GGTNACC 1 cut(s) 386
PspN4I GGNNCC 2 cut(s) 49, 399
PspPI GGNCC 1 cut(s) 19
PsuI RGATCY 1 cut(s) 397
RsaI GTAC 1 cut(s) 162
RsaNI GTAC 1 cut(s) 161
RseI CAYNNNNRTG 1 cut(s) 372
Rsr2I CGGWCCG 1 cut(s) 19
RsrII CGGWCCG 1 cut(s) 19
SacII CCGCGG 1 cut(s) 61
SaqAI TTAA 1 cut(s) 95
SatI GCNGC 2 cut(s) 61, 447
Sau3AI GATC 6 cut(s) 131, 219, 336, 397, 417, 424
Sau96I GGNCC 1 cut(s) 19
SchI GAGTC 1 cut(s) 428
SetI ASST 3 cut(s) 45, 258, 448
Sfr303I CCGCGG 1 cut(s) 61
SgrBI CCGCGG 1 cut(s) 61
SinI GGWCC 1 cut(s) 19
SmiMI CAYNNNNRTG 1 cut(s) 372
Sse9I AATT 3 cut(s) 472, 487, 493
SsiI CCGC 5 cut(s) 17, 58, 60, 428, 560
SspI AATATT 1 cut(s) 99
SspMI CTAG 1 cut(s) 248
TaaI ACNGT 1 cut(s) 371
TaqI TCGA 4 cut(s) 12, 54, 177, 416
TasI AATT 3 cut(s) 472, 487, 493
TatI WGTACW 1 cut(s) 160
TauI GCSGC 1 cut(s) 63
TfiI GAWTC 2 cut(s) 32, 145
Tru1I TTAA 1 cut(s) 95
Tru9I TTAA 1 cut(s) 95
TscAI CASTG 1 cut(s) 398
TseFI GTSAC 3 cut(s) 167, 365, 386
TseI GCWGC 1 cut(s) 446
Tsp45I GTSAC 3 cut(s) 167, 365, 386
TspDTI ATGAA 1 cut(s) 395
TspGWI ACGGA 2 cut(s) 97, 565
TspRI CASTG 1 cut(s) 398
VpaK11BI GGWCC 1 cut(s) 19
VspI ATTAAT 1 cut(s) 95
XapI RAATTY 1 cut(s) 487
XceI RCATGY 1 cut(s) 354
XspI CTAG 1 cut(s) 248
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.