Rw2G018840

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0005517
Physical Location & Seq
Reverse (-)
24782187 .. 24782825
639 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G018840.1

Sequence Viewer

Length: 486 bp
ATGGCAAAAATCGAAGCGGACCGGCGTGTTGAATCCGATGAGGTCGGCACCATCGACCGCGGCAAACATAAAGGGGAATCGCAACCACCATGTACAAAGTCACGATATCGAAAACGAAATGCTCATTGTTGTTGCAAGTGTCATGATCCCAAAACCCGACGCCGTGCCAAAACTAGGAAACGTCCTTTACCAGAGGGATTTGGTTATGTTCCTTCGGCAATATGTGTGGATAAGAATGGTAACTACATTCCAAGATTTTCTGATCGTGTTGCTTACATGTCTTGTCACCGTTGTGGTATGAAGGGTGACCACTGGGATCCATTTTGCCCCAACTCGATCAATGATCCGCCAGACTCAGGAGAAGCTGCTCCTACCCGTAACCCGTCATGGAATTATATATTGAGGAATTTCAATTTGAAAGAATATCTTGAGAAAAAGAATAATAAGACTGATGGGAAACCAAACCACCACGGAGGAAGACGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

161

Amino Acids

18.53

Weight (kDa)

9.63

Isoelectric Point (pI)

57.09

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 47
AccII CGCG 1 cut(s) 60
AciI CCGC 4 cut(s) 17, 58, 60, 347
AclWI GGATC 4 cut(s) 140, 311, 324, 338
AcsI RAATTY 1 cut(s) 406
AcyI GRCGYC 1 cut(s) 160
AfaI GTAC 1 cut(s) 94
AfiI CCNNNNNNNGG 2 cut(s) 174, 356
AflIII ACRYGT 1 cut(s) 276
AgsI TTSAA 3 cut(s) 32, 412, 418
AleI CACNNNNGTG 1 cut(s) 291
AluBI AGCT 1 cut(s) 365
AluI AGCT 1 cut(s) 365
AlwI GGATC 4 cut(s) 140, 311, 324, 338
ApeKI GCWGC 1 cut(s) 365
ApoI RAATTY 1 cut(s) 406
AspS9I GGNCC 1 cut(s) 19
AsuHPI GGTGA 2 cut(s) 278, 317
AvaII GGWCC 1 cut(s) 19
BamHI GGATCC 1 cut(s) 316
BanI GGYRCC 1 cut(s) 47
BbvI GCAGC 1 cut(s) 352
BccI CCATC 2 cut(s) 59, 446
BceAI ACGGC 1 cut(s) 147
BfaI CTAG 1 cut(s) 174
BisI GCNGC 2 cut(s) 61, 366
BlsI GCNGC 2 cut(s) 62, 367
Bme18I GGWCC 1 cut(s) 19
BmgT120I GGNCC 1 cut(s) 19
BmiI GGNNCC 2 cut(s) 49, 318
BmrI ACTGGG 1 cut(s) 322
BmuI ACTGGG 1 cut(s) 322
BpuEI CTTGAG 1 cut(s) 449
BsaHI GRCGYC 1 cut(s) 160
BsaJI CCNNGG 2 cut(s) 58, 469
Bsc4I CCNNNNNNNGG 2 cut(s) 174, 356
Bse118I RCCGGY 1 cut(s) 21
Bse1I ACTGG 1 cut(s) 317
BseDI CCNNGG 2 cut(s) 58, 469
BseLI CCNNNNNNNGG 2 cut(s) 174, 356
BseMII CTCAG 1 cut(s) 369
BseNI ACTGG 1 cut(s) 317
BseXI GCAGC 1 cut(s) 352
Bsh1236I CGCG 1 cut(s) 60
Bsh1285I CGRYCG 1 cut(s) 58
BshNI GGYRCC 1 cut(s) 47
BsiEI CGRYCG 1 cut(s) 58
BsiSI CCGG 1 cut(s) 22
BslI CCNNNNNNNGG 2 cut(s) 174, 356
Bsp1407I TGTACA 1 cut(s) 92
Bsp143I GATC 5 cut(s) 145, 262, 316, 336, 343
BspACI CCGC 4 cut(s) 17, 58, 60, 347
BspCNI CTCAG 1 cut(s) 368
BspFNI CGCG 1 cut(s) 60
BspHI TCATGA 1 cut(s) 142
BspLI GGNNCC 2 cut(s) 49, 318
BspPI GGATC 4 cut(s) 140, 311, 324, 338
BspT107I GGYRCC 1 cut(s) 47
BsrFI RCCGGY 1 cut(s) 21
BsrGI TGTACA 1 cut(s) 92
BsrI ACTGG 1 cut(s) 317
BssAI RCCGGY 1 cut(s) 21
BssECI CCNNGG 2 cut(s) 58, 469
BssMI GATC 5 cut(s) 145, 262, 316, 336, 343
BssNI GRCGYC 1 cut(s) 160
Bst4CI ACNGT 2 cut(s) 290, 483
BstACI GRCGYC 1 cut(s) 160
BstAUI TGTACA 1 cut(s) 92
BstDEI CTNAG 1 cut(s) 355
BstDSI CCRYGG 2 cut(s) 58, 469
BstEII GGTNACC 1 cut(s) 305
BstFNI CGCG 1 cut(s) 60
BstKTI GATC 5 cut(s) 148, 265, 319, 339, 346
BstMBI GATC 5 cut(s) 145, 262, 316, 336, 343
BstMCI CGRYCG 1 cut(s) 58
BstNSI RCATGY 1 cut(s) 280
BstPI GGTNACC 1 cut(s) 305
BstUI CGCG 1 cut(s) 60
BstV1I GCAGC 1 cut(s) 352
BstX2I RGATCY 1 cut(s) 316
BstYI RGATCY 1 cut(s) 316
BtgI CCRYGG 2 cut(s) 58, 469
BtsIMutI CAGTG 1 cut(s) 310
CciI TCATGA 1 cut(s) 142
Cfr10I RCCGGY 1 cut(s) 21
Cfr13I GGNCC 1 cut(s) 19
Cfr42I CCGCGG 1 cut(s) 61
CpoI CGGWCCG 1 cut(s) 19
CseI GACGC 1 cut(s) 168
Csp6I GTAC 1 cut(s) 93
CspCI CAANNNNNGTGG 2 cut(s) 207, 242
CspI CGGWCCG 1 cut(s) 19
CviAII CATG 4 cut(s) 90, 143, 277, 387
CviJI RGCY 1 cut(s) 365
CviKI_1 RGCY 1 cut(s) 365
CviQI GTAC 1 cut(s) 93
DdeI CTNAG 1 cut(s) 355
DpnI GATC 5 cut(s) 147, 264, 318, 338, 345
DpnII GATC 5 cut(s) 145, 262, 316, 336, 343
EciI GGCGGA 1 cut(s) 336
Eco32I GATATC 1 cut(s) 107
Eco47I GGWCC 1 cut(s) 19
Eco91I GGTNACC 1 cut(s) 305
EcoO65I GGTNACC 1 cut(s) 305
EcoRV GATATC 1 cut(s) 107
FaeI CATG 4 cut(s) 93, 146, 280, 390
FalI AAGNNNNNCTT 2 cut(s) 411, 443
FatI CATG 4 cut(s) 89, 142, 276, 386
Fnu4HI GCNGC 2 cut(s) 61, 366
Fsp4HI GCNGC 2 cut(s) 61, 366
FspBI CTAG 1 cut(s) 174
GluI GCNGC 2 cut(s) 61, 366
HapII CCGG 1 cut(s) 22
HgaI GACGC 1 cut(s) 168
Hin1I GRCGYC 1 cut(s) 160
Hin1II CATG 4 cut(s) 93, 146, 280, 390
HinfI GANTC 3 cut(s) 32, 77, 353
HpaII CCGG 1 cut(s) 22
HphI GGTGA 2 cut(s) 278, 317
Hpy188I TCNGA 2 cut(s) 37, 262
Hpy188III TCNNGA 4 cut(s) 102, 143, 357, 428
Hpy99I CGWCG 1 cut(s) 162
HpyAV CCTTC 2 cut(s) 222, 295
HpyCH4III ACNGT 2 cut(s) 290, 483
HpyCH4IV ACGT 1 cut(s) 181
HpyCH4V TGCA 1 cut(s) 135
HpyF3I CTNAG 1 cut(s) 355
HpySE526I ACGT 1 cut(s) 181
Hsp92I GRCGYC 1 cut(s) 160
Hsp92II CATG 4 cut(s) 93, 146, 280, 390
KspI CCGCGG 1 cut(s) 61
Kzo9I GATC 5 cut(s) 145, 262, 316, 336, 343
LmnI GCTCC 1 cut(s) 373
LpnPI CCDG 5 cut(s) 35, 204, 298, 342, 363
Lsp1109I GCAGC 1 cut(s) 352
MaeI CTAG 1 cut(s) 174
MaeII ACGT 1 cut(s) 181
MaeIII GTNAC 5 cut(s) 99, 239, 284, 305, 377
MalI GATC 5 cut(s) 147, 264, 318, 338, 345
MboI GATC 5 cut(s) 145, 262, 316, 336, 343
MflI RGATCY 1 cut(s) 316
MluCI AATT 3 cut(s) 391, 406, 412
MlyI GAGTC 1 cut(s) 347
MnlI CCTC 4 cut(s) 34, 187, 396, 467
MslI CAYNNNNRTG 1 cut(s) 291
MspA1I CMGCKG 1 cut(s) 60
MspI CCGG 1 cut(s) 22
MvnI CGCG 1 cut(s) 60
NdeII GATC 5 cut(s) 145, 262, 316, 336, 343
NlaIII CATG 4 cut(s) 93, 146, 280, 390
NlaIV GGNNCC 2 cut(s) 49, 318
NmuCI GTSAC 3 cut(s) 99, 284, 305
NspI RCATGY 1 cut(s) 280
OliI CACNNNNGTG 1 cut(s) 291
PagI TCATGA 1 cut(s) 142
PciI ACATGT 1 cut(s) 276
PcsI WCGNNNNNNNCGW 1 cut(s) 51
PfeI GAWTC 2 cut(s) 32, 77
PkrI GCNGC 2 cut(s) 62, 367
PleI GAGTC 1 cut(s) 347
PpsI GAGTC 1 cut(s) 347
PscI ACATGT 1 cut(s) 276
PspEI GGTNACC 1 cut(s) 305
PspN4I GGNNCC 2 cut(s) 49, 318
PspPI GGNCC 1 cut(s) 19
PsuI RGATCY 1 cut(s) 316
RsaI GTAC 1 cut(s) 94
RsaNI GTAC 1 cut(s) 93
RseI CAYNNNNRTG 1 cut(s) 291
Rsr2I CGGWCCG 1 cut(s) 19
RsrII CGGWCCG 1 cut(s) 19
SacII CCGCGG 1 cut(s) 61
SatI GCNGC 2 cut(s) 61, 366
Sau3AI GATC 5 cut(s) 145, 262, 316, 336, 343
Sau96I GGNCC 1 cut(s) 19
SchI GAGTC 1 cut(s) 347
SetI ASST 3 cut(s) 45, 184, 367
Sfr303I CCGCGG 1 cut(s) 61
SgrBI CCGCGG 1 cut(s) 61
SinI GGWCC 1 cut(s) 19
SmiMI CAYNNNNRTG 1 cut(s) 291
SmlI CTYRAG 1 cut(s) 428
SmoI CTYRAG 1 cut(s) 428
Sse9I AATT 3 cut(s) 391, 406, 412
SsiI CCGC 4 cut(s) 17, 58, 60, 347
SspMI CTAG 1 cut(s) 174
TaaI ACNGT 2 cut(s) 290, 483
TaiI ACGT 1 cut(s) 184
TaqI TCGA 4 cut(s) 12, 54, 109, 335
TasI AATT 3 cut(s) 391, 406, 412
TatI WGTACW 1 cut(s) 92
TauI GCSGC 1 cut(s) 63
TfiI GAWTC 2 cut(s) 32, 77
TscAI CASTG 1 cut(s) 317
TseFI GTSAC 3 cut(s) 99, 284, 305
TseI GCWGC 1 cut(s) 365
Tsp45I GTSAC 3 cut(s) 99, 284, 305
TspDTI ATGAA 1 cut(s) 314
TspGWI ACGGA 1 cut(s) 486
TspRI CASTG 1 cut(s) 317
VpaK11BI GGWCC 1 cut(s) 19
XapI RAATTY 1 cut(s) 406
XceI RCATGY 1 cut(s) 280
XspI CTAG 1 cut(s) 174
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.