FvH4_1g24220

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0004320
Physical Location & Seq
Forward (+)
16089292 .. 16089855
564 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g24220.t1

Sequence Viewer

Length: 564 bp
ATGACATCAAGCTCTATCTCTCTTGCTGCCTCCGCCTCCACGTCCGCCTCTACTCACGAGGCTTCAGCGGCTAAAGAGAAGATTCACATACGAGCTCCATACCCTTGGGCTACAACAAAGCGTGCCCTAGTATACTCTCGTCATTATTTACTCTCTCATGGCATCAACACAATCAGTGGTGCAGTTCAATGCAAAAAGTGTGATGAGAAGTATGAGATCGAGTACGATCTGCAACAAAAGTTTGCACAAGTGGCGACTTATGTGTCTCAAAACAAGAGTGCAATGCGTGATAGGGCACCTGCTGTTTGGATGACTCCCACACTCCCGGATTGCAAGTTCTGTGGGCAAACAAATTGTATGAAACCTCTGATAGACAAGAAGAAGTCGATCAATTGGTTGTTCCTGCTTTTAGGTCAAATGCTCGGAATGTGCAAACTCTCTCAATTGAAGTATTTCTGCAAACACACCAAGAATCACAGGACCGGTGCTAAAGATCGTGTTTTGTATCGTACTTATCTGGGACTGTTGAAACAACTTGAGCCGAATGGACCTTTTGATATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

188

Amino Acids

21.13

Weight (kDa)

9.56

Isoelectric Point (pI)

31.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7086 PF23324 48 - 179 3.7e-66 Domain of unknown function (DUF7086)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 307
AasI GACNNNNNNGTC 1 cut(s) 262
Acc36I ACCTGC 1 cut(s) 307
AccB1I GGYRCC 1 cut(s) 295
AccI GTMKAC 1 cut(s) 132
AciI CCGC 3 cut(s) 33, 45, 68
AcuI CTGAAG 1 cut(s) 48
AfaI GTAC 2 cut(s) 224, 511
AgeI ACCGGT 1 cut(s) 482
AgsI TTSAA 3 cut(s) 188, 448, 529
AjiI CACGTC 1 cut(s) 42
AloI GAACNNNNNNTCC 2 cut(s) 320, 352
AluBI AGCT 2 cut(s) 12, 95
AluI AGCT 2 cut(s) 12, 95
Alw21I GWGCWC 1 cut(s) 97
Alw26I GTCTC 1 cut(s) 270
ApeKI GCWGC 1 cut(s) 26
AsiGI ACCGGT 1 cut(s) 482
Asp700I GAANNNNTTC 1 cut(s) 452
AspS9I GGNCC 2 cut(s) 480, 548
AsuC2I CCSGG 1 cut(s) 326
AvaII GGWCC 2 cut(s) 480, 548
BaeGI GKGCMC 2 cut(s) 127, 298
BanI GGYRCC 1 cut(s) 295
BanII GRGCYC 1 cut(s) 97
BauI CACGAG 1 cut(s) 56
Bbv12I GWGCWC 1 cut(s) 97
BbvI GCAGC 1 cut(s) 13
BcnI CCSGG 1 cut(s) 326
BcoDI GTCTC 1 cut(s) 270
BfaI CTAG 1 cut(s) 128
BfuAI ACCTGC 1 cut(s) 307
BisI GCNGC 2 cut(s) 27, 69
BlsI GCNGC 2 cut(s) 28, 70
Bme1390I CCNGG 1 cut(s) 326
Bme18I GGWCC 2 cut(s) 480, 548
BmgBI CACGTC 1 cut(s) 42
BmgT120I GGNCC 2 cut(s) 480, 548
BmiI GGNNCC 1 cut(s) 297
BmrFI CCNGG 1 cut(s) 326
BmsI GCATC 1 cut(s) 171
BpuEI CTTGAG 1 cut(s) 557
BpuMI CCSGG 1 cut(s) 326
BsaJI CCNNGG 1 cut(s) 104
BsaWI WCCGGW 1 cut(s) 482
Bse118I RCCGGY 1 cut(s) 482
Bse3DI GCAATG 1 cut(s) 288
BseDI CCNNGG 1 cut(s) 104
BseGI GGATG 1 cut(s) 315
BseMI GCAATG 1 cut(s) 288
BseSI GKGCMC 2 cut(s) 127, 298
BseXI GCAGC 1 cut(s) 13
BsgI GTGCAG 1 cut(s) 201
BshNI GGYRCC 1 cut(s) 295
BshTI ACCGGT 1 cut(s) 482
BsiHKAI GWGCWC 1 cut(s) 97
BsiSI CCGG 2 cut(s) 326, 483
BslFI GGGAC 1 cut(s) 534
BsmAI GTCTC 1 cut(s) 270
BsmFI GGGAC 1 cut(s) 534
Bsp1286I GDGCHC 3 cut(s) 97, 127, 298
Bsp143I GATC 4 cut(s) 216, 226, 387, 493
BspACI CCGC 3 cut(s) 33, 45, 68
BspLI GGNNCC 1 cut(s) 297
BspMI ACCTGC 1 cut(s) 307
BspT107I GGYRCC 1 cut(s) 295
BsrDI GCAATG 1 cut(s) 288
BsrFI RCCGGY 1 cut(s) 482
BssAI RCCGGY 1 cut(s) 482
BssECI CCNNGG 1 cut(s) 104
BssMI GATC 4 cut(s) 216, 226, 387, 493
BssNAI GTATAC 1 cut(s) 133
BssSI CACGAG 1 cut(s) 56
BssT1I CCWWGG 1 cut(s) 104
Bst1107I GTATAC 1 cut(s) 133
Bst2BI CACGAG 1 cut(s) 56
Bst4CI ACNGT 1 cut(s) 525
BstC8I GCNNGC 1 cut(s) 123
BstF5I GGATG 1 cut(s) 315
BstKTI GATC 4 cut(s) 219, 229, 390, 496
BstMAI GTCTC 1 cut(s) 270
BstMBI GATC 4 cut(s) 216, 226, 387, 493
BstMWI GCNNNNNNNGC 3 cut(s) 32, 68, 251
BstSCI CCNGG 1 cut(s) 324
BstSLI GKGCMC 2 cut(s) 127, 298
BstV1I GCAGC 1 cut(s) 13
BstXI CCANNNNNNTGG 1 cut(s) 105
BstZ17I GTATAC 1 cut(s) 133
BtrI CACGTC 1 cut(s) 42
BtsCI GGATG 1 cut(s) 315
BtsIMutI CAGTG 1 cut(s) 181
BveI ACCTGC 1 cut(s) 307
Cac8I GCNNGC 1 cut(s) 123
Cfr10I RCCGGY 1 cut(s) 482
Cfr13I GGNCC 2 cut(s) 480, 548
Csp6I GTAC 2 cut(s) 223, 510
CspAI ACCGGT 1 cut(s) 482
CspCI CAANNNNNGTGG 2 cut(s) 322, 357
CviAII CATG 1 cut(s) 158
CviJI RGCY 6 cut(s) 12, 62, 71, 95, 110, 541
CviKI_1 RGCY 6 cut(s) 12, 62, 71, 95, 110, 541
CviQI GTAC 2 cut(s) 223, 510
DpnI GATC 4 cut(s) 218, 228, 389, 495
DpnII GATC 4 cut(s) 216, 226, 387, 493
DrdI GACNNNNNNGTC 1 cut(s) 262
DseDI GACNNNNNNGTC 1 cut(s) 262
EciI GGCGGA 2 cut(s) 22, 34
Ecl136II GAGCTC 1 cut(s) 95
Eco130I CCWWGG 1 cut(s) 104
Eco24I GRGCYC 1 cut(s) 97
Eco47I GGWCC 2 cut(s) 480, 548
Eco53kI GAGCTC 1 cut(s) 95
Eco57I CTGAAG 1 cut(s) 48
EcoICRI GAGCTC 1 cut(s) 95
EcoT14I CCWWGG 1 cut(s) 104
EcoT38I GRGCYC 1 cut(s) 97
ErhI CCWWGG 1 cut(s) 104
FaeI CATG 1 cut(s) 161
FaiI YATR 7 cut(s) 89, 100, 133, 159, 213, 261, 359
FaqI GGGAC 1 cut(s) 534
FatI CATG 1 cut(s) 157
FblI GTMKAC 1 cut(s) 132
Fnu4HI GCNGC 2 cut(s) 27, 69
FokI GGATG 1 cut(s) 322
FriOI GRGCYC 1 cut(s) 97
Fsp4HI GCNGC 2 cut(s) 27, 69
FspBI CTAG 1 cut(s) 128
GluI GCNGC 2 cut(s) 27, 69
HapII CCGG 2 cut(s) 326, 483
Hin1II CATG 1 cut(s) 161
HinfI GANTC 3 cut(s) 82, 313, 472
HpaII CCGG 2 cut(s) 326, 483
Hpy166II GTNNAC 1 cut(s) 133
Hpy188I TCNGA 2 cut(s) 369, 425
Hpy188III TCNNGA 1 cut(s) 56
Hpy8I GTNNAC 1 cut(s) 133
HpyCH4III ACNGT 1 cut(s) 525
HpyCH4IV ACGT 1 cut(s) 41
HpyCH4V TGCA 8 cut(s) 182, 192, 232, 245, 281, 333, 432, 459
HpyF10VI GCNNNNNNNGC 3 cut(s) 32, 68, 251
HpySE526I ACGT 1 cut(s) 41
Hsp92II CATG 1 cut(s) 161
Kzo9I GATC 4 cut(s) 216, 226, 387, 493
LmnI GCTCC 1 cut(s) 100
LpnPI CCDG 6 cut(s) 312, 339, 416, 463, 496, 503
Lsp1109I GCAGC 1 cut(s) 13
LweI GCATC 1 cut(s) 171
MaeI CTAG 1 cut(s) 128
MaeII ACGT 1 cut(s) 41
MalI GATC 4 cut(s) 218, 228, 389, 495
MboI GATC 4 cut(s) 216, 226, 387, 493
MboII GAAGA 2 cut(s) 91, 391
MfeI CAATTG 2 cut(s) 391, 443
MhlI GDGCHC 3 cut(s) 97, 127, 298
MluCI AATT 3 cut(s) 352, 391, 443
MlyI GAGTC 1 cut(s) 307
MnlI CCTC 5 cut(s) 40, 46, 52, 58, 375
MroXI GAANNNNTTC 1 cut(s) 452
MspA1I CMGCKG 1 cut(s) 68
MspI CCGG 2 cut(s) 326, 483
MspR9I CCNGG 1 cut(s) 326
MunI CAATTG 2 cut(s) 391, 443
MwoI GCNNNNNNNGC 3 cut(s) 32, 68, 251
NciI CCSGG 1 cut(s) 326
NdeII GATC 4 cut(s) 216, 226, 387, 493
NlaIII CATG 1 cut(s) 161
NlaIV GGNNCC 1 cut(s) 297
PaqCI CACCTGC 1 cut(s) 307
PdmI GAANNNNTTC 1 cut(s) 452
PfeI GAWTC 2 cut(s) 82, 472
PfoI TCCNGGA 1 cut(s) 324
PinAI ACCGGT 1 cut(s) 482
PkrI GCNGC 2 cut(s) 28, 70
PleI GAGTC 1 cut(s) 307
PpsI GAGTC 1 cut(s) 307
Psp124BI GAGCTC 1 cut(s) 97
PspN4I GGNNCC 1 cut(s) 297
PspPI GGNCC 2 cut(s) 480, 548
RsaI GTAC 2 cut(s) 224, 511
RsaNI GTAC 2 cut(s) 223, 510
SacI GAGCTC 1 cut(s) 97
SatI GCNGC 2 cut(s) 27, 69
Sau3AI GATC 4 cut(s) 216, 226, 387, 493
Sau96I GGNCC 2 cut(s) 480, 548
SchI GAGTC 1 cut(s) 307
ScrFI CCNGG 1 cut(s) 326
SduI GDGCHC 3 cut(s) 97, 127, 298
SetI ASST 7 cut(s) 14, 44, 97, 301, 367, 415, 553
SfaNI GCATC 1 cut(s) 171
SinI GGWCC 2 cut(s) 480, 548
SmlI CTYRAG 1 cut(s) 536
SmoI CTYRAG 1 cut(s) 536
Sse9I AATT 3 cut(s) 352, 391, 443
SsiI CCGC 3 cut(s) 33, 45, 68
SspMI CTAG 1 cut(s) 128
SstI GAGCTC 1 cut(s) 97
StyD4I CCNGG 1 cut(s) 324
StyI CCWWGG 1 cut(s) 104
TaaI ACNGT 1 cut(s) 525
TaiI ACGT 1 cut(s) 44
TaqI TCGA 2 cut(s) 219, 386
TasI AATT 3 cut(s) 352, 391, 443
TauI GCSGC 1 cut(s) 71
TfiI GAWTC 2 cut(s) 82, 472
TscAI CASTG 1 cut(s) 181
TseI GCWGC 1 cut(s) 26
TspDTI ATGAA 1 cut(s) 374
TspRI CASTG 1 cut(s) 181
VpaK11BI GGWCC 2 cut(s) 480, 548
XmiI GTMKAC 1 cut(s) 132
XmnI GAANNNNTTC 1 cut(s) 452
XspI CTAG 1 cut(s) 128
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.