MD04G1118400.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0004320
Physical Location & Seq
Forward (+)
20420694 .. 20420948
255 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1118400.v1.1.491

Sequence Viewer

Length: 255 bp
ATGAATCCCACCTTACCCAATTGCAAGTTTTGTGATCAGAACAACTGTATGAAGCCAGTGATTTCGAAGAAGCGTTCGATCAATTGGTTGTTTTTGTTGCTGGGGCAAATGCTTGGGTGCTGCAAGCTTTCTGAGTTGAAGTATTTTTGTAAGCACACAAAGAATCACAGGACCGGCGCTAAAGATCGAGTCTTGTATCTCACTTATTTGGGACTTTGCAAACAGCTTGATCCCTATGGACCCTTTGAGATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

85

Amino Acids

9.77

Weight (kDa)

9.3

Isoelectric Point (pI)

30.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7086 PF23324 1 - 76 3.2e-41 Domain of unknown function (DUF7086)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 224
AgsI TTSAA 1 cut(s) 139
AluBI AGCT 2 cut(s) 127, 226
AluI AGCT 2 cut(s) 127, 226
AlwI GGATC 1 cut(s) 224
ApeKI GCWGC 1 cut(s) 120
AspLEI GCGC 1 cut(s) 179
AspS9I GGNCC 2 cut(s) 171, 239
AsuII TTCGAA 1 cut(s) 65
AvaII GGWCC 2 cut(s) 171, 239
BbvI GCAGC 1 cut(s) 107
BclI TGATCA 1 cut(s) 34
BfoI RGCGCY 1 cut(s) 180
BisI GCNGC 1 cut(s) 121
BlsI GCNGC 1 cut(s) 122
Bme18I GGWCC 2 cut(s) 171, 239
BmgT120I GGNCC 2 cut(s) 171, 239
BmiI GGNNCC 1 cut(s) 241
Bpu14I TTCGAA 1 cut(s) 65
Bse118I RCCGGY 1 cut(s) 173
Bse1I ACTGG 1 cut(s) 56
BseMII CTCAG 1 cut(s) 123
BseNI ACTGG 1 cut(s) 56
BseXI GCAGC 1 cut(s) 107
BseYI CCCAGC 1 cut(s) 100
BsiSI CCGG 1 cut(s) 174
BslFI GGGAC 1 cut(s) 225
BsmFI GGGAC 1 cut(s) 225
Bsp119I TTCGAA 1 cut(s) 65
Bsp143I GATC 4 cut(s) 34, 78, 184, 229
BspCNI CTCAG 1 cut(s) 124
BspLI GGNNCC 1 cut(s) 241
BspPI GGATC 1 cut(s) 224
BspT104I TTCGAA 1 cut(s) 65
BsrFI RCCGGY 1 cut(s) 173
BsrI ACTGG 1 cut(s) 56
BssAI RCCGGY 1 cut(s) 173
BssMI GATC 4 cut(s) 34, 78, 184, 229
Bst4CI ACNGT 1 cut(s) 47
BstBI TTCGAA 1 cut(s) 65
BstC8I GCNNGC 1 cut(s) 125
BstDEI CTNAG 1 cut(s) 132
BstH2I RGCGCY 1 cut(s) 180
BstHHI GCGC 1 cut(s) 179
BstKTI GATC 4 cut(s) 37, 81, 187, 232
BstMBI GATC 4 cut(s) 34, 78, 184, 229
BstV1I GCAGC 1 cut(s) 107
BtsIMutI CAGTG 1 cut(s) 63
Cac8I GCNNGC 1 cut(s) 125
CfoI GCGC 1 cut(s) 179
Cfr10I RCCGGY 1 cut(s) 173
Cfr13I GGNCC 2 cut(s) 171, 239
CviJI RGCY 3 cut(s) 55, 127, 226
CviKI_1 RGCY 3 cut(s) 55, 127, 226
DdeI CTNAG 1 cut(s) 132
DpnI GATC 4 cut(s) 36, 80, 186, 231
DpnII GATC 4 cut(s) 34, 78, 184, 229
Eco47I GGWCC 2 cut(s) 171, 239
FaiI YATR 2 cut(s) 50, 237
FaqI GGGAC 1 cut(s) 225
FbaI TGATCA 1 cut(s) 34
Fnu4HI GCNGC 1 cut(s) 121
Fsp4HI GCNGC 1 cut(s) 121
GlaI GCGC 1 cut(s) 178
GluI GCNGC 1 cut(s) 121
GsaI CCCAGC 1 cut(s) 104
HaeII RGCGCY 1 cut(s) 180
HapII CCGG 1 cut(s) 174
HhaI GCGC 1 cut(s) 179
Hin6I GCGC 1 cut(s) 177
HinP1I GCGC 1 cut(s) 177
HindIII AAGCTT 1 cut(s) 125
HinfI GANTC 3 cut(s) 4, 163, 189
HpaII CCGG 1 cut(s) 174
Hpy188I TCNGA 2 cut(s) 39, 133
HpyCH4III ACNGT 1 cut(s) 47
HpyCH4V TGCA 3 cut(s) 24, 123, 219
HpyF3I CTNAG 1 cut(s) 132
HspAI GCGC 1 cut(s) 177
Ksp22I TGATCA 1 cut(s) 34
Kzo9I GATC 4 cut(s) 34, 78, 184, 229
LpnPI CCDG 4 cut(s) 69, 86, 154, 187
Lsp1109I GCAGC 1 cut(s) 107
MalI GATC 4 cut(s) 36, 80, 186, 231
MboI GATC 4 cut(s) 34, 78, 184, 229
MboII GAAGA 1 cut(s) 79
MfeI CAATTG 2 cut(s) 19, 82
MluCI AATT 2 cut(s) 19, 82
MlyI GAGTC 1 cut(s) 198
MseI TTAA 1 cut(s) 253
MspI CCGG 1 cut(s) 174
MunI CAATTG 2 cut(s) 19, 82
NdeII GATC 4 cut(s) 34, 78, 184, 229
NlaIV GGNNCC 1 cut(s) 241
NspV TTCGAA 1 cut(s) 65
PfeI GAWTC 2 cut(s) 4, 163
PkrI GCNGC 1 cut(s) 122
PleI GAGTC 1 cut(s) 197
PpsI GAGTC 1 cut(s) 197
PspFI CCCAGC 1 cut(s) 100
PspN4I GGNNCC 1 cut(s) 241
PspPI GGNCC 2 cut(s) 171, 239
SaqAI TTAA 1 cut(s) 253
SatI GCNGC 1 cut(s) 121
Sau3AI GATC 4 cut(s) 34, 78, 184, 229
Sau96I GGNCC 2 cut(s) 171, 239
SchI GAGTC 1 cut(s) 198
SetI ASST 3 cut(s) 14, 129, 228
SfuI TTCGAA 1 cut(s) 65
SinI GGWCC 2 cut(s) 171, 239
Sse9I AATT 2 cut(s) 19, 82
TaaI ACNGT 1 cut(s) 47
TaqI TCGA 3 cut(s) 65, 77, 187
TasI AATT 2 cut(s) 19, 82
TfiI GAWTC 2 cut(s) 4, 163
Tru1I TTAA 1 cut(s) 253
Tru9I TTAA 1 cut(s) 253
TscAI CASTG 1 cut(s) 63
TseI GCWGC 1 cut(s) 120
TspDTI ATGAA 2 cut(s) 17, 65
TspRI CASTG 1 cut(s) 63
VpaK11BI GGWCC 2 cut(s) 171, 239
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.