RLG00000024477

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0004320
Physical Location & Seq
Forward (+)
36242543 .. 36243316
774 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000024477

Sequence Viewer

Length: 774 bp
ATGACTTCCTCCATACCTTTCGATCACATCAATGAAGAACACGAAGAGCTGCTAACCCTTCAACTCTCCACCGGAAACCATTCAACACCTATCCAACAATCTCCTCTTTTGATCTCGACACCGTCTCAGTCTCTAACTTTATTTGCAGCAACGAATTTCCACATGACATCACCACAAAACTCTAACCCTCCTGCTCCTGCTGCCTCTAATCACGAGGCTTCAGCGGCTGGTAGTTCCAGGTCGTCCCGTGCCCGTAGATCTTCAGCTCAAACCCTAAGAGAAGGGAAGACTGAAGACATACCAGCCCCATATCCTTGGGCTACCACAAAACGTGCTCAAGTATACTCTCGTCGATATTTGCTTTCTAATGGCATCAAGAAAATCAGTGGTGCGGTCCAATGCAAAAAGTGTGATCAGAAGTATGAGATTGAGTATGATCTGGAACAAAAGTTCGATGAAGTGGCGACTTATGTGTCTCAGCACAAGAGTGCAATGCGTGATAGGGCACCTGCAGTTTGGATGACTCCCACACTCCCGGATTGCAAGTTCTGTGGTCAAACAAATTGCGTGAAGCCTGAGATGGACAAGAAGAGGTCAATCAATTGGCTGTTCTTGCTTTTGGGTCAAATGCTCGGAATGTGCAAACTCAATGAATTGAAGTACTTCTGCAAGCACACCAAGAATCACAGGACCGGTGCTAAAGATCGGGTATTGTATCTTACTTATCTGGGATTGTTGAAACAACTTGAGCCGAATGGACCCTTTGATATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

258

Amino Acids

28.95

Weight (kDa)

9.1

Isoelectric Point (pI)

52.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7086 PF23324 118 - 249 4.2e-65 Domain of unknown function (DUF7086)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 517
AasI GACNNNNNNGTC 1 cut(s) 472
Acc36I ACCTGC 1 cut(s) 517
AccB1I GGYRCC 1 cut(s) 505
AccI GTMKAC 1 cut(s) 342
AciI CCGC 2 cut(s) 224, 392
AcsI RAATTY 1 cut(s) 154
AcuI CTGAAG 3 cut(s) 204, 246, 312
AfaI GTAC 1 cut(s) 662
AgeI ACCGGT 1 cut(s) 692
AgsI TTSAA 4 cut(s) 62, 84, 658, 739
AjnI CCWGG 1 cut(s) 236
AleI CACNNNNGTG 1 cut(s) 486
AloI GAACNNNNNNTCC 4 cut(s) 434, 466, 530, 562
AluBI AGCT 2 cut(s) 49, 266
AluI AGCT 2 cut(s) 49, 266
Alw21I GWGCWC 1 cut(s) 337
Alw26I GTCTC 3 cut(s) 129, 135, 480
AlwNI CAGNNNCTG 1 cut(s) 227
ApeKI GCWGC 3 cut(s) 49, 146, 200
ApoI RAATTY 1 cut(s) 154
AsiGI ACCGGT 1 cut(s) 692
Asp700I GAANNNNTTC 2 cut(s) 79, 662
AspS9I GGNCC 3 cut(s) 394, 690, 758
AsuC2I CCSGG 1 cut(s) 536
AsuHPI GGTGA 1 cut(s) 162
AvaII GGWCC 3 cut(s) 394, 690, 758
BaeGI GKGCMC 2 cut(s) 253, 508
BanI GGYRCC 1 cut(s) 505
BauI CACGAG 1 cut(s) 212
BbsI GAAGAC 2 cut(s) 293, 300
Bbv12I GWGCWC 1 cut(s) 337
BbvI GCAGC 3 cut(s) 36, 158, 187
BccI CCATC 1 cut(s) 574
BciT130I CCWGG 1 cut(s) 238
BclI TGATCA 1 cut(s) 412
BcnI CCSGG 1 cut(s) 536
BcoDI GTCTC 3 cut(s) 129, 135, 480
BfmI CTRYAG 1 cut(s) 510
BfuAI ACCTGC 1 cut(s) 517
BglII AGATCT 1 cut(s) 257
BisI GCNGC 4 cut(s) 50, 147, 201, 225
BlsI GCNGC 4 cut(s) 51, 148, 202, 226
BmcAI AGTACT 1 cut(s) 662
Bme1390I CCNGG 2 cut(s) 238, 536
Bme18I GGWCC 3 cut(s) 394, 690, 758
BmgT120I GGNCC 3 cut(s) 394, 690, 758
BmiI GGNNCC 2 cut(s) 507, 760
BmrFI CCNGG 2 cut(s) 238, 536
BmsI GCATC 1 cut(s) 381
BpiI GAAGAC 2 cut(s) 293, 300
BpuEI CTTGAG 2 cut(s) 321, 767
BpuMI CCSGG 1 cut(s) 536
BsaJI CCNNGG 1 cut(s) 314
BsaWI WCCGGW 2 cut(s) 71, 692
Bse118I RCCGGY 1 cut(s) 692
Bse3DI GCAATG 1 cut(s) 498
BseBI CCWGG 1 cut(s) 238
BseDI CCNNGG 1 cut(s) 314
BseGI GGATG 1 cut(s) 525
BseMI GCAATG 1 cut(s) 498
BseMII CTCAG 3 cut(s) 140, 491, 567
BseRI GAGGAG 1 cut(s) 93
BseSI GKGCMC 2 cut(s) 253, 508
BseXI GCAGC 3 cut(s) 36, 158, 187
BshNI GGYRCC 1 cut(s) 505
BshTI ACCGGT 1 cut(s) 692
BsiHKAI GWGCWC 1 cut(s) 337
BsiSI CCGG 3 cut(s) 72, 536, 693
BslFI GGGAC 1 cut(s) 229
BsmAI GTCTC 3 cut(s) 129, 135, 480
BsmBI CGTCTC 1 cut(s) 129
BsmFI GGGAC 1 cut(s) 229
Bsp1286I GDGCHC 3 cut(s) 253, 337, 508
Bsp143I GATC 6 cut(s) 22, 111, 257, 412, 436, 703
BspACI CCGC 2 cut(s) 224, 392
BspCNI CTCAG 3 cut(s) 139, 490, 568
BspLI GGNNCC 2 cut(s) 507, 760
BspMAI CTGCAG 1 cut(s) 514
BspMI ACCTGC 1 cut(s) 517
BspQI GCTCTTC 1 cut(s) 39
BspT107I GGYRCC 1 cut(s) 505
BsrDI GCAATG 1 cut(s) 498
BsrFI RCCGGY 1 cut(s) 692
BssAI RCCGGY 1 cut(s) 692
BssECI CCNNGG 1 cut(s) 314
BssMI GATC 6 cut(s) 22, 111, 257, 412, 436, 703
BssNAI GTATAC 1 cut(s) 343
BssSI CACGAG 1 cut(s) 212
BssT1I CCWWGG 1 cut(s) 314
Bst1107I GTATAC 1 cut(s) 343
Bst2BI CACGAG 1 cut(s) 212
Bst2UI CCWGG 1 cut(s) 238
Bst4CI ACNGT 1 cut(s) 123
Bst6I CTCTTC 2 cut(s) 39, 584
BstC8I GCNNGC 1 cut(s) 671
BstDEI CTNAG 4 cut(s) 126, 275, 477, 576
BstF5I GGATG 1 cut(s) 525
BstKTI GATC 6 cut(s) 25, 114, 260, 415, 439, 706
BstMAI GTCTC 3 cut(s) 129, 135, 480
BstMBI GATC 6 cut(s) 22, 111, 257, 412, 436, 703
BstMWI GCNNNNNNNGC 3 cut(s) 200, 224, 613
BstNI CCWGG 1 cut(s) 238
BstSCI CCNGG 2 cut(s) 236, 534
BstSFI CTRYAG 1 cut(s) 510
BstSLI GKGCMC 2 cut(s) 253, 508
BstV1I GCAGC 3 cut(s) 36, 158, 187
BstV2I GAAGAC 2 cut(s) 293, 300
BstX2I RGATCY 1 cut(s) 257
BstXI CCANNNNNNTGG 1 cut(s) 315
BstYI RGATCY 1 cut(s) 257
BstZ17I GTATAC 1 cut(s) 343
BtsCI GGATG 1 cut(s) 525
BtsIMutI CAGTG 1 cut(s) 391
BveI ACCTGC 1 cut(s) 517
Cac8I GCNNGC 1 cut(s) 671
CaiI CAGNNNCTG 1 cut(s) 227
Cfr10I RCCGGY 1 cut(s) 692
Cfr13I GGNCC 3 cut(s) 394, 690, 758
Csp6I GTAC 1 cut(s) 661
CspAI ACCGGT 1 cut(s) 692
CspCI CAANNNNNGTGG 2 cut(s) 532, 567
CviAII CATG 1 cut(s) 163
CviJI RGCY 9 cut(s) 49, 218, 227, 266, 305, 320, 574, 607, 751
CviKI_1 RGCY 9 cut(s) 49, 218, 227, 266, 305, 320, 574, 607, 751
CviQI GTAC 1 cut(s) 661
DdeI CTNAG 4 cut(s) 126, 275, 477, 576
DpnI GATC 6 cut(s) 24, 113, 259, 414, 438, 705
DpnII GATC 6 cut(s) 22, 111, 257, 412, 436, 703
DrdI GACNNNNNNGTC 1 cut(s) 472
DseDI GACNNNNNNGTC 1 cut(s) 472
Eam1104I CTCTTC 2 cut(s) 39, 584
EarI CTCTTC 2 cut(s) 39, 584
Eco130I CCWWGG 1 cut(s) 314
Eco47I GGWCC 3 cut(s) 394, 690, 758
Eco57I CTGAAG 3 cut(s) 204, 246, 312
EcoRII CCWGG 1 cut(s) 236
EcoT14I CCWWGG 1 cut(s) 314
ErhI CCWWGG 1 cut(s) 314
Esp3I CGTCTC 1 cut(s) 129
FaeI CATG 1 cut(s) 166
FaiI YATR 8 cut(s) 14, 164, 299, 310, 343, 423, 435, 471
FaqI GGGAC 1 cut(s) 229
FatI CATG 1 cut(s) 162
FbaI TGATCA 1 cut(s) 412
FblI GTMKAC 1 cut(s) 342
Fnu4HI GCNGC 4 cut(s) 50, 147, 201, 225
FokI GGATG 1 cut(s) 532
Fsp4HI GCNGC 4 cut(s) 50, 147, 201, 225
GluI GCNGC 4 cut(s) 50, 147, 201, 225
HapII CCGG 3 cut(s) 72, 536, 693
Hin1II CATG 1 cut(s) 166
HinfI GANTC 2 cut(s) 523, 682
HpaII CCGG 3 cut(s) 72, 536, 693
HphI GGTGA 1 cut(s) 162
Hpy166II GTNNAC 1 cut(s) 343
Hpy188I TCNGA 2 cut(s) 417, 635
Hpy188III TCNNGA 4 cut(s) 115, 212, 376, 440
Hpy8I GTNNAC 1 cut(s) 343
Hpy99I CGWCG 1 cut(s) 354
HpyAV CCTTC 2 cut(s) 68, 275
HpyCH4III ACNGT 1 cut(s) 123
HpyCH4IV ACGT 1 cut(s) 331
HpyCH4V TGCA 7 cut(s) 146, 402, 491, 512, 543, 642, 669
HpyF10VI GCNNNNNNNGC 3 cut(s) 200, 224, 613
HpyF3I CTNAG 4 cut(s) 126, 275, 477, 576
HpySE526I ACGT 1 cut(s) 331
Hsp92II CATG 1 cut(s) 166
Ksp22I TGATCA 1 cut(s) 412
Kzo9I GATC 6 cut(s) 22, 111, 257, 412, 436, 703
LguI GCTCTTC 1 cut(s) 39
LmnI GCTCC 1 cut(s) 199
Lsp1109I GCAGC 3 cut(s) 36, 158, 187
LweI GCATC 1 cut(s) 381
MaeII ACGT 1 cut(s) 331
MalI GATC 6 cut(s) 24, 113, 259, 414, 438, 705
MboI GATC 6 cut(s) 22, 111, 257, 412, 436, 703
MboII GAAGA 6 cut(s) 47, 56, 252, 298, 305, 601
MfeI CAATTG 1 cut(s) 601
MflI RGATCY 1 cut(s) 257
MhlI GDGCHC 3 cut(s) 253, 337, 508
MluCI AATT 4 cut(s) 154, 562, 601, 653
MlyI GAGTC 1 cut(s) 517
MmeI TCCRAC 1 cut(s) 118
MnlI CCTC 6 cut(s) 19, 114, 198, 208, 214, 585
MroXI GAANNNNTTC 2 cut(s) 79, 662
MslI CAYNNNNRTG 2 cut(s) 30, 486
MspA1I CMGCKG 1 cut(s) 224
MspI CCGG 3 cut(s) 72, 536, 693
MspR9I CCNGG 2 cut(s) 238, 536
MunI CAATTG 1 cut(s) 601
MvaI CCWGG 1 cut(s) 238
MwoI GCNNNNNNNGC 3 cut(s) 200, 224, 613
NciI CCSGG 1 cut(s) 536
NdeII GATC 6 cut(s) 22, 111, 257, 412, 436, 703
NlaIII CATG 1 cut(s) 166
NlaIV GGNNCC 2 cut(s) 507, 760
OliI CACNNNNGTG 1 cut(s) 486
PaqCI CACCTGC 1 cut(s) 517
PciSI GCTCTTC 1 cut(s) 39
PdmI GAANNNNTTC 2 cut(s) 79, 662
PfeI GAWTC 1 cut(s) 682
PflFI GACNNNGTC 1 cut(s) 121
PfoI TCCNGGA 1 cut(s) 534
PinAI ACCGGT 1 cut(s) 692
PkrI GCNGC 4 cut(s) 51, 148, 202, 226
PleI GAGTC 1 cut(s) 517
PpsI GAGTC 1 cut(s) 517
Psp6I CCWGG 1 cut(s) 236
PspGI CCWGG 1 cut(s) 236
PspN4I GGNNCC 2 cut(s) 507, 760
PspPI GGNCC 3 cut(s) 394, 690, 758
PstI CTGCAG 1 cut(s) 514
PstNI CAGNNNCTG 1 cut(s) 227
PsuI RGATCY 1 cut(s) 257
PsyI GACNNNGTC 1 cut(s) 121
RsaI GTAC 1 cut(s) 662
RsaNI GTAC 1 cut(s) 661
RseI CAYNNNNRTG 2 cut(s) 30, 486
SapI GCTCTTC 1 cut(s) 39
SatI GCNGC 4 cut(s) 50, 147, 201, 225
Sau3AI GATC 6 cut(s) 22, 111, 257, 412, 436, 703
Sau96I GGNCC 3 cut(s) 394, 690, 758
ScaI AGTACT 1 cut(s) 662
SchI GAGTC 1 cut(s) 517
ScrFI CCNGG 2 cut(s) 238, 536
SduI GDGCHC 3 cut(s) 253, 337, 508
SetI ASST 8 cut(s) 19, 51, 91, 242, 268, 334, 511, 596
SfaNI GCATC 1 cut(s) 381
SfcI CTRYAG 1 cut(s) 510
SinI GGWCC 3 cut(s) 394, 690, 758
SmiMI CAYNNNNRTG 2 cut(s) 30, 486
SmlI CTYRAG 2 cut(s) 336, 746
SmoI CTYRAG 2 cut(s) 336, 746
Sse9I AATT 4 cut(s) 154, 562, 601, 653
SsiI CCGC 2 cut(s) 224, 392
StyD4I CCNGG 2 cut(s) 236, 534
StyI CCWWGG 1 cut(s) 314
TaaI ACNGT 1 cut(s) 123
TaiI ACGT 1 cut(s) 334
TaqI TCGA 4 cut(s) 21, 116, 352, 453
TasI AATT 4 cut(s) 154, 562, 601, 653
TatI WGTACW 1 cut(s) 660
TauI GCSGC 1 cut(s) 227
TfiI GAWTC 1 cut(s) 682
TscAI CASTG 1 cut(s) 391
TseI GCWGC 3 cut(s) 49, 146, 200
TspDTI ATGAA 3 cut(s) 48, 471, 666
TspRI CASTG 1 cut(s) 391
Tth111I GACNNNGTC 1 cut(s) 121
VpaK11BI GGWCC 3 cut(s) 394, 690, 758
XapI RAATTY 1 cut(s) 154
XmiI GTMKAC 1 cut(s) 342
XmnI GAANNNNTTC 2 cut(s) 79, 662
ZrmI AGTACT 1 cut(s) 662
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.