Rorug03G0089400

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0004320
Physical Location & Seq
Forward (+)
6968854 .. 6970177
1324 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0089400.1

Sequence Viewer

Length: 561 bp
ATGTATCGCACGCCTTTGGAGCTAACGCTGGTCTCCGCCCATGACATCAAGTACGTCAACCTCTTCTCCAAGATGAACGTCTACGCCGTCGTTTCGATCACCGGCGGCGCCAAGAAGCAGAAGCTCAAGACCCCCGTCCACAAGGGAGGTGGCACCAATCCCAAGTGGAACCACTCCCTCAAGTTCACGCTCGACGACGCCGCTCTCAACCAGGACCACCTCAAAGTCGAGATTCAGCTCGTCTCCGACCGCACCCTCCGCGACACCAAGATCGGAAAAGTCGTTATTCCGGCAAAGGAGTTGCTTCAGTATTCCGGCACGGACGTGAGCTACCAGCTTGTGACGCGCAAGGGCAGATCCAAAGGGAGCGTCAGCTTCAGGTACAAGTTGGGCGAGAAATACTGGGTTGAGCCCGAGCCAAAACCCGAGCCCGTCATGGCTTACGCCGCTTACCCCGCCGCGGGTTACGGAAACCCACCAGTTGTGCAGCGGCCGAAGAAGTCCAAAAAAGGAAAACTGACGCTTGGTTTGTTTGCTTTGATAATTATGGGTGGATTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

186

Amino Acids

20.66

Weight (kDa)

9.92

Isoelectric Point (pI)

23.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 6 - 113 5.8e-17 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 107, 152
AccBSI CCGCTC 1 cut(s) 203
AccI GTMKAC 1 cut(s) 81
AccII CGCG 3 cut(s) 261, 346, 461
AclWI GGATC 1 cut(s) 351
AcoI YGGCCR 1 cut(s) 491
AcuI CTGAAG 2 cut(s) 290, 361
AcyI GRCGYC 2 cut(s) 108, 198
AfaI GTAC 2 cut(s) 53, 383
AfiI CCNNNNNNNGG 2 cut(s) 460, 461
AjiI CACGTC 1 cut(s) 325
AjnI CCWGG 1 cut(s) 210
AleI CACNNNNGTG 1 cut(s) 323
AluBI AGCT 6 cut(s) 22, 124, 238, 330, 337, 375
AluI AGCT 6 cut(s) 22, 124, 238, 330, 337, 375
Alw26I GTCTC 2 cut(s) 37, 247
AlwI GGATC 1 cut(s) 351
Ama87I CYCGRG 2 cut(s) 413, 425
AoxI GGCC 1 cut(s) 491
ApeKI GCWGC 1 cut(s) 487
ArsI GACNNNNNNTTYG 4 cut(s) 354, 386, 511, 543
AspLEI GCGC 2 cut(s) 110, 348
AspS9I GGNCC 1 cut(s) 214
AsuHPI GGTGA 1 cut(s) 91
AvaI CYCGRG 2 cut(s) 413, 425
AvaII GGWCC 1 cut(s) 214
BanI GGYRCC 2 cut(s) 107, 152
BanII GRGCYC 2 cut(s) 414, 432
BbvI GCAGC 1 cut(s) 499
BceAI ACGGC 1 cut(s) 71
BciT130I CCWGG 1 cut(s) 212
BcoDI GTCTC 2 cut(s) 37, 247
BfaI CTAG 1 cut(s) 559
BfoI RGCGCY 1 cut(s) 111
BisI GCNGC 6 cut(s) 106, 201, 447, 459, 488, 491
BlsI GCNGC 6 cut(s) 107, 202, 448, 460, 489, 492
Bme1390I CCNGG 1 cut(s) 212
Bme18I GGWCC 1 cut(s) 214
BmeT110I CYCGRG 2 cut(s) 413, 425
BmgBI CACGTC 1 cut(s) 325
BmgT120I GGNCC 1 cut(s) 214
BmiI GGNNCC 3 cut(s) 109, 154, 170
BmrFI CCNGG 1 cut(s) 212
BmrI ACTGGG 1 cut(s) 412
BmuI ACTGGG 1 cut(s) 412
BoxI GACNNNNGTC 1 cut(s) 134
BpuEI CTTGAG 2 cut(s) 110, 164
BsaHI GRCGYC 2 cut(s) 108, 198
BsaI GGTCTC 1 cut(s) 37
BsaJI CCNNGG 1 cut(s) 459
BsaXI ACNNNNNCTCC 2 cut(s) 50, 80
Bsc4I CCNNNNNNNGG 2 cut(s) 460, 461
Bse118I RCCGGY 1 cut(s) 101
Bse1I ACTGG 2 cut(s) 407, 479
BseBI CCWGG 1 cut(s) 212
BseDI CCNNGG 1 cut(s) 459
BseLI CCNNNNNNNGG 2 cut(s) 460, 461
BseNI ACTGG 2 cut(s) 407, 479
BseX3I CGGCCG 1 cut(s) 491
BseXI GCAGC 1 cut(s) 499
BsgI GTGCAG 1 cut(s) 506
Bsh1236I CGCG 3 cut(s) 261, 346, 461
Bsh1285I CGRYCG 2 cut(s) 250, 494
BshFI GGCC 1 cut(s) 493
BshNI GGYRCC 2 cut(s) 107, 152
BsiEI CGRYCG 2 cut(s) 250, 494
BsiHKCI CYCGRG 2 cut(s) 413, 425
BsiSI CCGG 3 cut(s) 102, 290, 315
BslI CCNNNNNNNGG 2 cut(s) 460, 461
BsmAI GTCTC 2 cut(s) 37, 247
BsmBI CGTCTC 1 cut(s) 247
BsnI GGCC 1 cut(s) 493
Bso31I GGTCTC 1 cut(s) 37
BsoBI CYCGRG 2 cut(s) 413, 425
Bsp1286I GDGCHC 2 cut(s) 414, 432
Bsp143I GATC 3 cut(s) 96, 270, 356
BspANI GGCC 1 cut(s) 493
BspFNI CGCG 3 cut(s) 261, 346, 461
BspLI GGNNCC 3 cut(s) 109, 154, 170
BspPI GGATC 1 cut(s) 351
BspT107I GGYRCC 2 cut(s) 107, 152
BspTNI GGTCTC 1 cut(s) 37
BsrBI CCGCTC 1 cut(s) 203
BsrFI RCCGGY 1 cut(s) 101
BsrI ACTGG 2 cut(s) 407, 479
BssAI RCCGGY 1 cut(s) 101
BssECI CCNNGG 1 cut(s) 459
BssMI GATC 3 cut(s) 96, 270, 356
BssNI GRCGYC 2 cut(s) 108, 198
Bst2UI CCWGG 1 cut(s) 212
Bst6I CTCTTC 1 cut(s) 68
BstACI GRCGYC 2 cut(s) 108, 198
BstC8I GCNNGC 1 cut(s) 11
BstDSI CCRYGG 1 cut(s) 459
BstFNI CGCG 3 cut(s) 261, 346, 461
BstH2I RGCGCY 1 cut(s) 111
BstHHI GCGC 2 cut(s) 110, 348
BstKTI GATC 3 cut(s) 99, 273, 359
BstMAI GTCTC 2 cut(s) 37, 247
BstMBI GATC 3 cut(s) 96, 270, 356
BstMCI CGRYCG 2 cut(s) 250, 494
BstMWI GCNNNNNNNGC 5 cut(s) 19, 258, 343, 446, 455
BstNI CCWGG 1 cut(s) 212
BstPAI GACNNNNGTC 1 cut(s) 134
BstSCI CCNGG 1 cut(s) 210
BstUI CGCG 3 cut(s) 261, 346, 461
BstV1I GCAGC 1 cut(s) 499
BstX2I RGATCY 1 cut(s) 356
BstYI RGATCY 1 cut(s) 356
BstZI CGGCCG 1 cut(s) 491
BsuRI GGCC 1 cut(s) 493
BtgI CCRYGG 1 cut(s) 459
BtrI CACGTC 1 cut(s) 325
Cac8I GCNNGC 1 cut(s) 11
CfoI GCGC 2 cut(s) 110, 348
Cfr10I RCCGGY 1 cut(s) 101
Cfr13I GGNCC 1 cut(s) 214
Cfr42I CCGCGG 1 cut(s) 462
CseI GACGC 4 cut(s) 206, 352, 358, 529
Csp6I GTAC 2 cut(s) 52, 382
CspCI CAANNNNNGTGG 2 cut(s) 130, 165
CviAII CATG 2 cut(s) 41, 436
CviQI GTAC 2 cut(s) 52, 382
DinI GGCGCC 1 cut(s) 109
DpnI GATC 3 cut(s) 98, 272, 358
DpnII GATC 3 cut(s) 96, 270, 356
EaeI YGGCCR 1 cut(s) 491
EagI CGGCCG 1 cut(s) 491
Eam1104I CTCTTC 1 cut(s) 68
EarI CTCTTC 1 cut(s) 68
EciI GGCGGA 1 cut(s) 25
EclXI CGGCCG 1 cut(s) 491
Eco24I GRGCYC 2 cut(s) 414, 432
Eco31I GGTCTC 1 cut(s) 37
Eco47I GGWCC 1 cut(s) 214
Eco52I CGGCCG 1 cut(s) 491
Eco57I CTGAAG 2 cut(s) 290, 361
Eco88I CYCGRG 2 cut(s) 413, 425
EcoRII CCWGG 1 cut(s) 210
EcoT38I GRGCYC 2 cut(s) 414, 432
EgeI GGCGCC 1 cut(s) 109
EheI GGCGCC 1 cut(s) 109
Esp3I CGTCTC 1 cut(s) 247
FaeI CATG 2 cut(s) 44, 439
FaiI YATR 3 cut(s) 42, 437, 548
FatI CATG 2 cut(s) 40, 435
FauI CCCGC 2 cut(s) 454, 463
FblI GTMKAC 1 cut(s) 81
Fnu4HI GCNGC 6 cut(s) 106, 201, 447, 459, 488, 491
FriOI GRGCYC 2 cut(s) 414, 432
Fsp4HI GCNGC 6 cut(s) 106, 201, 447, 459, 488, 491
FspBI CTAG 1 cut(s) 559
GlaI GCGC 2 cut(s) 109, 347
GluI GCNGC 6 cut(s) 106, 201, 447, 459, 488, 491
HaeII RGCGCY 1 cut(s) 111
HaeIII GGCC 1 cut(s) 493
HapII CCGG 3 cut(s) 102, 290, 315
HgaI GACGC 4 cut(s) 206, 352, 358, 529
HhaI GCGC 2 cut(s) 110, 348
Hin1I GRCGYC 2 cut(s) 108, 198
Hin1II CATG 2 cut(s) 44, 439
Hin6I GCGC 2 cut(s) 108, 346
HinP1I GCGC 2 cut(s) 108, 346
HincII GTYRAC 1 cut(s) 58
HindII GTYRAC 1 cut(s) 58
HinfI GANTC 2 cut(s) 232, 555
HpaII CCGG 3 cut(s) 102, 290, 315
HphI GGTGA 1 cut(s) 91
Hpy166II GTNNAC 4 cut(s) 58, 82, 139, 186
Hpy188I TCNGA 2 cut(s) 247, 275
Hpy188III TCNNGA 2 cut(s) 127, 229
Hpy8I GTNNAC 4 cut(s) 58, 82, 139, 186
Hpy99I CGWCG 3 cut(s) 92, 197, 200
HpyCH4IV ACGT 3 cut(s) 54, 78, 324
HpyCH4V TGCA 1 cut(s) 487
HpyF10VI GCNNNNNNNGC 5 cut(s) 19, 258, 343, 446, 455
HpySE526I ACGT 3 cut(s) 54, 78, 324
Hsp92I GRCGYC 2 cut(s) 108, 198
Hsp92II CATG 2 cut(s) 44, 439
HspAI GCGC 2 cut(s) 108, 346
KasI GGCGCC 1 cut(s) 107
KspI CCGCGG 1 cut(s) 462
Kzo9I GATC 3 cut(s) 96, 270, 356
LmnI GCTCC 2 cut(s) 19, 366
Lsp1109I GCAGC 1 cut(s) 499
MaeI CTAG 1 cut(s) 559
MaeII ACGT 3 cut(s) 54, 78, 324
MaeIII GTNAC 2 cut(s) 340, 464
MalI GATC 3 cut(s) 98, 272, 358
MbiI CCGCTC 1 cut(s) 203
MboI GATC 3 cut(s) 96, 270, 356
MboII GAAGA 2 cut(s) 55, 508
MflI RGATCY 1 cut(s) 356
MhlI GDGCHC 2 cut(s) 414, 432
MluCI AATT 1 cut(s) 543
Mly113I GGCGCC 1 cut(s) 108
MmeI TCCRAC 1 cut(s) 270
MnlI CCTC 5 cut(s) 71, 140, 188, 230, 266
MslI CAYNNNNRTG 1 cut(s) 323
MspA1I CMGCKG 2 cut(s) 461, 490
MspI CCGG 3 cut(s) 102, 290, 315
MspR9I CCNGG 1 cut(s) 212
MvaI CCWGG 1 cut(s) 212
MvnI CGCG 3 cut(s) 261, 346, 461
MwoI GCNNNNNNNGC 5 cut(s) 19, 258, 343, 446, 455
NarI GGCGCC 1 cut(s) 108
NdeII GATC 3 cut(s) 96, 270, 356
NlaIII CATG 2 cut(s) 44, 439
NlaIV GGNNCC 3 cut(s) 109, 154, 170
NmuCI GTSAC 1 cut(s) 340
OliI CACNNNNGTG 1 cut(s) 323
PcsI WCGNNNNNNNCGW 2 cut(s) 84, 279
PfeI GAWTC 2 cut(s) 232, 555
PkrI GCNGC 6 cut(s) 107, 202, 448, 460, 489, 492
PluTI GGCGCC 1 cut(s) 111
PshAI GACNNNNGTC 1 cut(s) 134
Psp6I CCWGG 1 cut(s) 210
PspGI CCWGG 1 cut(s) 210
PspN4I GGNNCC 3 cut(s) 109, 154, 170
PspPI GGNCC 1 cut(s) 214
PsuI RGATCY 1 cut(s) 356
RsaI GTAC 2 cut(s) 53, 383
RsaNI GTAC 2 cut(s) 52, 382
RseI CAYNNNNRTG 1 cut(s) 323
SacII CCGCGG 1 cut(s) 462
SatI GCNGC 6 cut(s) 106, 201, 447, 459, 488, 491
Sau3AI GATC 3 cut(s) 96, 270, 356
Sau96I GGNCC 1 cut(s) 214
ScrFI CCNGG 1 cut(s) 212
SduI GDGCHC 2 cut(s) 414, 432
SfoI GGCGCC 1 cut(s) 109
Sfr303I CCGCGG 1 cut(s) 462
SgrAI CRCCGGYG 1 cut(s) 101
SgrBI CCGCGG 1 cut(s) 462
SinI GGWCC 1 cut(s) 214
SmiMI CAYNNNNRTG 1 cut(s) 323
SmlI CTYRAG 2 cut(s) 125, 179
SmoI CTYRAG 2 cut(s) 125, 179
Sse9I AATT 1 cut(s) 543
SspDI GGCGCC 1 cut(s) 107
SspMI CTAG 1 cut(s) 559
StyD4I CCNGG 1 cut(s) 210
TaiI ACGT 3 cut(s) 57, 81, 327
TaqI TCGA 3 cut(s) 95, 192, 228
TasI AATT 1 cut(s) 543
TauI GCSGC 5 cut(s) 108, 203, 449, 461, 493
TfiI GAWTC 2 cut(s) 232, 555
TseFI GTSAC 1 cut(s) 340
TseI GCWGC 1 cut(s) 487
Tsp45I GTSAC 1 cut(s) 340
TspDTI ATGAA 1 cut(s) 89
TspGWI ACGGA 2 cut(s) 335, 483
VpaK11BI GGWCC 1 cut(s) 214
XcmI CCANNNNNNNNNTGG 1 cut(s) 146
XmiI GTMKAC 1 cut(s) 81
XspI CTAG 1 cut(s) 559
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.