MD10G1078300.v1.1

Hydroxyproline-rich glycoprotein family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0004320
Physical Location & Seq
Forward (+)
11191468 .. 11192289
822 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1078300.v1.1.491

Sequence Viewer

Length: 822 bp
ATGCTCGCTCTCTCGCTCAACTCTAATCGTCCTCAATCAAGACCTCGTCTAATGCCATCGGTAGATACACAGCTACCAGCATCGTTGCCACTGCCGGTGCCACCACAATCATCGCCGTCACTGTCAGTGCAGCCAACACCCTTGTTCATACAAACCCTAATGCCTCAAAAACATTCATCTCATCCTCTCCAACTCTCATCCTCACACCCACTTTACATGACTAAACTACAATCTTCCTCCTCCCCGTCCATGCCCTTGCCCAATCCCGGAGTTCCCACATCTCGTCATCTGCGTGCACGGCGGAACCCTACTCAAGGTCCGAGGGAGGGAAAGAGCGAGACTGTCCCGGCTCCATTTCCGTGGGCTACCACAAGGCGGGCCATGGTTCACAGTCTGGAATACTTGCATTCAAGGCAAATCGTTACCATCACTGGTGAAGTGCAGTGCAAGCGTTGTGAACGACAATATGAGATGGATTATAATCTGAAGGAAAAGTTTCTTGAGGTTGGGAGTTTTATTGCTGGTAACAAGAGCATGATGCGCGATAGGGCACCGAGTGTGTGGATGAACCCGGTTCTTCCTGCATGCAAGTTTTGCAAACAAGAGAACAGCGCGAAACCGATCATAGGGGACAAGAAGAAGGTGATTAACTGGCTGTTTTTGCTGCTGGGGCAGATGCTTGGTTGCTGCACGCTTGAGCAGTTGAAATATTTCTGTAAGCACACCAAAAATCATCGAACCGGTGCCAAGGATCGAGTTCTTTATCTTACCTATCTGGGTTTGTGTAAACAACTCGATCCCAATGGACCTTTTGATCGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

274

Amino Acids

30.84

Weight (kDa)

9.88

Isoelectric Point (pI)

60.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7086 PF23324 132 - 265 1.1e-67 Domain of unknown function (DUF7086)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 480
AccB1I GGYRCC 3 cut(s) 97, 550, 743
AccII CGCG 2 cut(s) 543, 614
AciI CCGC 2 cut(s) 301, 376
AclWI GGATC 2 cut(s) 759, 791
AcuI CTGAAG 1 cut(s) 506
AdeI CACNNNGTG 1 cut(s) 557
AfiI CCNNNNNNNGG 5 cut(s) 266, 314, 326, 375, 626
AgeI ACCGGT 1 cut(s) 740
AgsI TTSAA 2 cut(s) 411, 706
AluBI AGCT 1 cut(s) 73
AluI AGCT 1 cut(s) 73
Alw21I GWGCWC 1 cut(s) 298
Alw26I GTCTC 1 cut(s) 332
Alw44I GTGCAC 1 cut(s) 294
AlwI GGATC 2 cut(s) 759, 791
AoxI GGCC 1 cut(s) 378
ApaLI GTGCAC 1 cut(s) 294
ApeKI GCWGC 3 cut(s) 130, 664, 687
AsiGI ACCGGT 1 cut(s) 740
Asp700I GAANNNNTTC 2 cut(s) 495, 710
AspLEI GCGC 2 cut(s) 543, 614
AspS9I GGNCC 3 cut(s) 317, 378, 806
AsuC2I CCSGG 3 cut(s) 267, 347, 572
AsuHPI GGTGA 2 cut(s) 446, 655
AvaII GGWCC 2 cut(s) 317, 806
BaeGI GKGCMC 2 cut(s) 298, 553
BanI GGYRCC 3 cut(s) 97, 550, 743
Bbv12I GWGCWC 1 cut(s) 298
BbvI GCAGC 3 cut(s) 142, 651, 674
BccI CCATC 3 cut(s) 64, 434, 466
BceAI ACGGC 2 cut(s) 100, 314
BcnI CCSGG 3 cut(s) 267, 347, 572
BcoDI GTCTC 1 cut(s) 332
BisI GCNGC 3 cut(s) 131, 665, 688
BlsI GCNGC 3 cut(s) 132, 666, 689
Bme1390I CCNGG 3 cut(s) 267, 347, 572
Bme18I GGWCC 2 cut(s) 317, 806
BmgT120I GGNCC 3 cut(s) 317, 378, 806
BmiI GGNNCC 5 cut(s) 99, 305, 351, 552, 745
BmrFI CCNGG 3 cut(s) 267, 347, 572
BmsI GCATC 3 cut(s) 89, 528, 666
BpuEI CTTGAG 3 cut(s) 297, 521, 716
BpuMI CCSGG 3 cut(s) 267, 347, 572
Bsa29I ATCGAT 1 cut(s) 817
BsaBI GATNNNNATC 1 cut(s) 480
BsaJI CCNNGG 4 cut(s) 320, 359, 381, 747
BsaWI WCCGGW 1 cut(s) 740
Bsc4I CCNNNNNNNGG 5 cut(s) 266, 314, 326, 375, 626
Bse118I RCCGGY 2 cut(s) 94, 740
Bse1I ACTGG 2 cut(s) 436, 656
Bse8I GATNNNNATC 1 cut(s) 480
BseCI ATCGAT 1 cut(s) 817
BseDI CCNNGG 4 cut(s) 320, 359, 381, 747
BseGI GGATG 3 cut(s) 181, 197, 570
BseJI GATNNNNATC 1 cut(s) 480
BseLI CCNNNNNNNGG 5 cut(s) 266, 314, 326, 375, 626
BseNI ACTGG 2 cut(s) 436, 656
BseRI GAGGAG 1 cut(s) 229
BseSI GKGCMC 2 cut(s) 298, 553
BseXI GCAGC 3 cut(s) 142, 651, 674
BseYI CCCAGC 1 cut(s) 667
BsgI GTGCAG 3 cut(s) 149, 461, 673
Bsh1236I CGCG 2 cut(s) 543, 614
BshFI GGCC 1 cut(s) 380
BshNI GGYRCC 3 cut(s) 97, 550, 743
BshTI ACCGGT 1 cut(s) 740
BshVI ATCGAT 1 cut(s) 817
BsiHKAI GWGCWC 1 cut(s) 298
BsiSI CCGG 5 cut(s) 95, 267, 347, 572, 741
BslFI GGGAC 2 cut(s) 329, 644
BslI CCNNNNNNNGG 5 cut(s) 266, 314, 326, 375, 626
BsmAI GTCTC 1 cut(s) 332
BsmFI GGGAC 2 cut(s) 329, 644
BsmI GAATGC 1 cut(s) 406
BsnI GGCC 1 cut(s) 380
Bsp1286I GDGCHC 2 cut(s) 298, 553
Bsp143I GATC 4 cut(s) 621, 751, 796, 814
Bsp19I CCATGG 1 cut(s) 381
BspACI CCGC 2 cut(s) 301, 376
BspANI GGCC 1 cut(s) 380
BspDI ATCGAT 1 cut(s) 817
BspFNI CGCG 2 cut(s) 543, 614
BspLI GGNNCC 5 cut(s) 99, 305, 351, 552, 745
BspPI GGATC 2 cut(s) 759, 791
BspT107I GGYRCC 3 cut(s) 97, 550, 743
BsrFI RCCGGY 2 cut(s) 94, 740
BsrI ACTGG 2 cut(s) 436, 656
BssAI RCCGGY 2 cut(s) 94, 740
BssECI CCNNGG 4 cut(s) 320, 359, 381, 747
BssMI GATC 4 cut(s) 621, 751, 796, 814
BssT1I CCWWGG 2 cut(s) 381, 747
Bst4CI ACNGT 3 cut(s) 123, 343, 392
BstAPI GCANNNNNTGC 1 cut(s) 594
BstC8I GCNNGC 6 cut(s) 6, 294, 378, 449, 586, 692
BstDSI CCRYGG 2 cut(s) 359, 381
BstENI CCTNNNNNAGG 1 cut(s) 312
BstF5I GGATG 3 cut(s) 181, 197, 570
BstFNI CGCG 2 cut(s) 543, 614
BstHHI GCGC 2 cut(s) 543, 614
BstKTI GATC 4 cut(s) 624, 754, 799, 817
BstMAI GTCTC 1 cut(s) 332
BstMBI GATC 4 cut(s) 621, 751, 796, 814
BstMWI GCNNNNNNNGC 7 cut(s) 298, 412, 448, 540, 594, 661, 670
BstNSI RCATGY 1 cut(s) 588
BstSCI CCNGG 3 cut(s) 265, 345, 570
BstSLI GKGCMC 2 cut(s) 298, 553
BstUI CGCG 2 cut(s) 543, 614
BstV1I GCAGC 3 cut(s) 142, 651, 674
BstXI CCANNNNNNTGG 1 cut(s) 360
Bsu15I ATCGAT 1 cut(s) 817
BsuRI GGCC 1 cut(s) 380
BsuTUI ATCGAT 1 cut(s) 817
BtgI CCRYGG 2 cut(s) 359, 381
BtgZI GCGATG 1 cut(s) 96
BtsCI GGATG 3 cut(s) 181, 197, 570
BtsI GCAGTG 2 cut(s) 89, 449
BtsIMutI CAGTG 5 cut(s) 89, 119, 132, 429, 449
Cac8I GCNNGC 6 cut(s) 6, 294, 378, 449, 586, 692
CfoI GCGC 2 cut(s) 543, 614
Cfr10I RCCGGY 2 cut(s) 94, 740
Cfr13I GGNCC 3 cut(s) 317, 378, 806
ClaI ATCGAT 1 cut(s) 817
CspAI ACCGGT 1 cut(s) 740
CviAII CATG 5 cut(s) 217, 250, 382, 535, 585
CviJI RGCY 6 cut(s) 73, 133, 350, 365, 380, 655
CviKI_1 RGCY 6 cut(s) 73, 133, 350, 365, 380, 655
DpnI GATC 4 cut(s) 623, 753, 798, 816
DpnII GATC 4 cut(s) 621, 751, 796, 814
DraIII CACNNNGTG 1 cut(s) 557
EciI GGCGGA 1 cut(s) 316
Eco130I CCWWGG 2 cut(s) 381, 747
Eco47I GGWCC 2 cut(s) 317, 806
Eco57I CTGAAG 1 cut(s) 506
EcoNI CCTNNNNNAGG 1 cut(s) 312
EcoT14I CCWWGG 2 cut(s) 381, 747
ErhI CCWWGG 2 cut(s) 381, 747
FaeI CATG 5 cut(s) 220, 253, 385, 538, 588
FaiI YATR 9 cut(s) 149, 218, 251, 383, 468, 480, 536, 586, 626
FaqI GGGAC 2 cut(s) 329, 644
FatI CATG 5 cut(s) 216, 249, 381, 534, 584
FauI CCCGC 1 cut(s) 369
Fnu4HI GCNGC 3 cut(s) 131, 665, 688
FokI GGATG 3 cut(s) 168, 184, 577
Fsp4HI GCNGC 3 cut(s) 131, 665, 688
GlaI GCGC 2 cut(s) 542, 613
GluI GCNGC 3 cut(s) 131, 665, 688
GsaI CCCAGC 1 cut(s) 671
HaeIII GGCC 1 cut(s) 380
HapII CCGG 5 cut(s) 95, 267, 347, 572, 741
HhaI GCGC 2 cut(s) 543, 614
Hin1II CATG 5 cut(s) 220, 253, 385, 538, 588
Hin6I GCGC 2 cut(s) 541, 612
HinP1I GCGC 2 cut(s) 541, 612
HpaII CCGG 5 cut(s) 95, 267, 347, 572, 741
HphI GGTGA 2 cut(s) 446, 655
Hpy166II GTNNAC 4 cut(s) 296, 388, 458, 788
Hpy188I TCNGA 2 cut(s) 321, 486
Hpy188III TCNNGA 3 cut(s) 39, 395, 500
Hpy8I GTNNAC 4 cut(s) 296, 388, 458, 788
HpyAV CCTTC 2 cut(s) 481, 634
HpyCH4III ACNGT 3 cut(s) 123, 343, 392
HpyCH4V TGCA 9 cut(s) 130, 296, 406, 442, 447, 584, 588, 597, 690
HpyF10VI GCNNNNNNNGC 7 cut(s) 298, 412, 448, 540, 594, 661, 670
Hsp92II CATG 5 cut(s) 220, 253, 385, 538, 588
HspAI GCGC 2 cut(s) 541, 612
Kzo9I GATC 4 cut(s) 621, 751, 796, 814
LmnI GCTCC 1 cut(s) 355
Lsp1109I GCAGC 3 cut(s) 142, 651, 674
LweI GCATC 3 cut(s) 89, 528, 666
MaeIII GTNAC 3 cut(s) 117, 421, 524
MalI GATC 4 cut(s) 623, 753, 798, 816
MboI GATC 4 cut(s) 621, 751, 796, 814
MboII GAAGA 3 cut(s) 225, 569, 649
MhlI GDGCHC 2 cut(s) 298, 553
MmeI TCCRAC 1 cut(s) 214
MroXI GAANNNNTTC 2 cut(s) 495, 710
MseI TTAA 1 cut(s) 648
MslI CAYNNNNRTG 2 cut(s) 291, 358
MspI CCGG 5 cut(s) 95, 267, 347, 572, 741
MspR9I CCNGG 3 cut(s) 267, 347, 572
Mva1269I GAATGC 1 cut(s) 406
MvnI CGCG 2 cut(s) 543, 614
MwoI GCNNNNNNNGC 7 cut(s) 298, 412, 448, 540, 594, 661, 670
NciI CCSGG 3 cut(s) 267, 347, 572
NcoI CCATGG 1 cut(s) 381
NdeII GATC 4 cut(s) 621, 751, 796, 814
NlaIII CATG 5 cut(s) 220, 253, 385, 538, 588
NlaIV GGNNCC 5 cut(s) 99, 305, 351, 552, 745
NmuCI GTSAC 1 cut(s) 117
NspI RCATGY 1 cut(s) 588
PaeI GCATGC 1 cut(s) 588
PcsI WCGNNNNNNNCGW 1 cut(s) 289
PctI GAATGC 1 cut(s) 406
PdmI GAANNNNTTC 2 cut(s) 495, 710
PflFI GACNNNGTC 1 cut(s) 45
PfoI TCCNGGA 1 cut(s) 265
PinAI ACCGGT 1 cut(s) 740
PkrI GCNGC 3 cut(s) 132, 666, 689
PsiI TTATAA 1 cut(s) 480
PspFI CCCAGC 1 cut(s) 667
PspN4I GGNNCC 5 cut(s) 99, 305, 351, 552, 745
PspPI GGNCC 3 cut(s) 317, 378, 806
PsyI GACNNNGTC 1 cut(s) 45
RseI CAYNNNNRTG 2 cut(s) 291, 358
SaqAI TTAA 1 cut(s) 648
SatI GCNGC 3 cut(s) 131, 665, 688
Sau3AI GATC 4 cut(s) 621, 751, 796, 814
Sau96I GGNCC 3 cut(s) 317, 378, 806
ScrFI CCNGG 3 cut(s) 267, 347, 572
SduI GDGCHC 2 cut(s) 298, 553
SetI ASST 7 cut(s) 46, 75, 319, 507, 645, 773, 811
SfaNI GCATC 3 cut(s) 89, 528, 666
SinI GGWCC 2 cut(s) 317, 806
SmiMI CAYNNNNRTG 2 cut(s) 291, 358
SmlI CTYRAG 3 cut(s) 312, 500, 695
SmoI CTYRAG 3 cut(s) 312, 500, 695
SphI GCATGC 1 cut(s) 588
SsiI CCGC 2 cut(s) 301, 376
SspI AATATT 1 cut(s) 710
StyD4I CCNGG 3 cut(s) 265, 345, 570
StyI CCWWGG 2 cut(s) 381, 747
TaaI ACNGT 3 cut(s) 123, 343, 392
TaqI TCGA 4 cut(s) 736, 754, 795, 817
Tru1I TTAA 1 cut(s) 648
Tru9I TTAA 1 cut(s) 648
TscAI CASTG 5 cut(s) 96, 126, 132, 436, 449
TseFI GTSAC 1 cut(s) 117
TseI GCWGC 3 cut(s) 130, 664, 687
Tsp45I GTSAC 1 cut(s) 117
TspDTI ATGAA 3 cut(s) 136, 165, 581
TspGWI ACGGA 1 cut(s) 348
TspRI CASTG 5 cut(s) 96, 126, 132, 436, 449
Tth111I GACNNNGTC 1 cut(s) 45
VneI GTGCAC 1 cut(s) 294
VpaK11BI GGWCC 2 cut(s) 317, 806
XagI CCTNNNNNAGG 1 cut(s) 312
XceI RCATGY 1 cut(s) 588
XmnI GAANNNNTTC 2 cut(s) 495, 710
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.