FvH4_2g08300

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0001583
Physical Location & Seq
Reverse (-)
7210520 .. 7214438
3919 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g08300.t1

Sequence Viewer

Length: 558 bp
ATGGCTTTCCCAGGCGAGGTCGGATTGCGGGTACTCTTCTCCCCTCTCGGTTCCAACGTCGTCGTCCGCACTGCTTGCTGCTCTGTGGGGGTCGCTTTACCTGTTTACTCAACTTTTAAAGCTATTGAGAGCAAAGATCATAACGAGCAGCAGCGCTGGCTTCTTTACTGGGCTGCGTATGGATCTTTCAGTATCGCAGAAGTGTTTTCAGACAAACTTCTCTCCTGGGTTCCGCTCTACTATCAGATGAAGTTTGCATTTCTGGTTTGGTTACAGCTTCCAACTGCAAATGGGGCAAAGCATTTGTATATGAGGCATATGCGTCCGTTCCTGCTGAGGCATCAAGCACGACTTGATCAAGTTGTTGAGTTTATATATGCTGAAACGAGTAAATTCATCATTTCACACCAAGGAGAATTACTGTTTGCAAGGGATCTTTTTATGAAAATTATGGGCTCAGTTAGGGAACTTGTGCACCCAGGACGAAAACAATCAAGTCATGCAATTCGAGGCCCTGAAGAACAAAGTGAGAACTCTGGATCAGATAAAGACGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2 PfkB TB2_DP1_HVA22

Protein Analysis

186

Amino Acids

21.16

Weight (kDa)

8.54

Isoelectric Point (pI)

46.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TB2_DP1_HVA22 PF03134 34 - 110 2e-28 TB2/DP1, HVA22 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 235
AciI CCGC 3 cut(s) 28, 67, 233
AclWI GGATC 3 cut(s) 190, 441, 547
AcsI RAATTY 1 cut(s) 392
AcuI CTGAAG 1 cut(s) 537
AfaI GTAC 1 cut(s) 33
AfeI AGCGCT 1 cut(s) 155
AfiI CCNNNNNNNGG 1 cut(s) 16
AjnI CCWGG 3 cut(s) 10, 224, 478
AluBI AGCT 2 cut(s) 122, 277
AluI AGCT 2 cut(s) 122, 277
Alw21I GWGCWC 1 cut(s) 477
Alw44I GTGCAC 1 cut(s) 473
AlwI GGATC 3 cut(s) 190, 441, 547
Aor51HI AGCGCT 1 cut(s) 155
AoxI GGCC 1 cut(s) 511
ApaLI GTGCAC 1 cut(s) 473
ApeKI GCWGC 4 cut(s) 78, 148, 151, 173
ApoI RAATTY 1 cut(s) 392
AspLEI GCGC 1 cut(s) 156
AspS9I GGNCC 1 cut(s) 512
BaeGI GKGCMC 1 cut(s) 477
BanII GRGCYC 1 cut(s) 458
Bbv12I GWGCWC 1 cut(s) 477
BbvCI CCTCAGC 1 cut(s) 335
BbvI GCAGC 4 cut(s) 65, 160, 160, 163
BciT130I CCWGG 3 cut(s) 12, 226, 480
BclI TGATCA 1 cut(s) 355
BfoI RGCGCY 1 cut(s) 157
BisI GCNGC 4 cut(s) 79, 149, 152, 174
BlsI GCNGC 4 cut(s) 80, 150, 153, 175
Bme1390I CCNGG 3 cut(s) 12, 226, 480
BmgT120I GGNCC 1 cut(s) 512
BmiI GGNNCC 2 cut(s) 52, 231
BmrFI CCNGG 3 cut(s) 12, 226, 480
BmrI ACTGGG 1 cut(s) 178
BmsI GCATC 1 cut(s) 349
BmuI ACTGGG 1 cut(s) 178
Bpu10I CCTNAGC 1 cut(s) 335
BsaJI CCNNGG 4 cut(s) 10, 225, 409, 478
Bsc4I CCNNNNNNNGG 1 cut(s) 16
Bse1I ACTGG 1 cut(s) 173
BseBI CCWGG 3 cut(s) 12, 226, 480
BseDI CCNNGG 4 cut(s) 10, 225, 409, 478
BseLI CCNNNNNNNGG 1 cut(s) 16
BseMII CTCAG 2 cut(s) 326, 471
BseNI ACTGG 1 cut(s) 173
BseSI GKGCMC 1 cut(s) 477
BseXI GCAGC 4 cut(s) 65, 160, 160, 163
BshFI GGCC 1 cut(s) 513
BsiHKAI GWGCWC 1 cut(s) 477
BslI CCNNNNNNNGG 1 cut(s) 16
BsnI GGCC 1 cut(s) 513
Bsp1286I GDGCHC 2 cut(s) 458, 477
Bsp143I GATC 5 cut(s) 136, 182, 355, 433, 539
BspACI CCGC 3 cut(s) 28, 67, 233
BspANI GGCC 1 cut(s) 513
BspCNI CTCAG 2 cut(s) 327, 470
BspLI GGNNCC 2 cut(s) 52, 231
BspPI GGATC 3 cut(s) 190, 441, 547
BsrBI CCGCTC 1 cut(s) 235
BsrI ACTGG 1 cut(s) 173
BssECI CCNNGG 4 cut(s) 10, 225, 409, 478
BssMI GATC 5 cut(s) 136, 182, 355, 433, 539
BssT1I CCWWGG 1 cut(s) 409
Bst2UI CCWGG 3 cut(s) 12, 226, 480
Bst4CI ACNGT 1 cut(s) 423
Bst6I CTCTTC 1 cut(s) 41
BstAPI GCANNNNNTGC 1 cut(s) 75
BstC8I GCNNGC 2 cut(s) 76, 158
BstDEI CTNAG 2 cut(s) 335, 457
BstH2I RGCGCY 1 cut(s) 157
BstHHI GCGC 1 cut(s) 156
BstKTI GATC 5 cut(s) 139, 185, 358, 436, 542
BstMBI GATC 5 cut(s) 136, 182, 355, 433, 539
BstMWI GCNNNNNNNGC 3 cut(s) 75, 157, 293
BstNI CCWGG 3 cut(s) 12, 226, 480
BstSCI CCNGG 3 cut(s) 10, 224, 478
BstSLI GKGCMC 1 cut(s) 477
BstV1I GCAGC 4 cut(s) 65, 160, 160, 163
BstX2I RGATCY 2 cut(s) 182, 433
BstYI RGATCY 2 cut(s) 182, 433
BsuRI GGCC 1 cut(s) 513
BtsI GCAGTG 1 cut(s) 69
BtsIMutI CAGTG 1 cut(s) 69
Cac8I GCNNGC 2 cut(s) 76, 158
CfoI GCGC 1 cut(s) 156
Cfr13I GGNCC 1 cut(s) 512
CseI GACGC 1 cut(s) 311
Csp6I GTAC 1 cut(s) 32
CviAII CATG 1 cut(s) 500
CviJI RGCY 7 cut(s) 5, 122, 160, 173, 277, 456, 513
CviKI_1 RGCY 7 cut(s) 5, 122, 160, 173, 277, 456, 513
CviQI GTAC 1 cut(s) 32
DdeI CTNAG 2 cut(s) 335, 457
DpnI GATC 5 cut(s) 138, 184, 357, 435, 541
DpnII GATC 5 cut(s) 136, 182, 355, 433, 539
DraI TTTAAA 1 cut(s) 118
Eam1104I CTCTTC 1 cut(s) 41
EarI CTCTTC 1 cut(s) 41
Eco130I CCWWGG 1 cut(s) 409
Eco24I GRGCYC 1 cut(s) 458
Eco47III AGCGCT 1 cut(s) 155
Eco57I CTGAAG 1 cut(s) 537
EcoO109I RGGNCCY 1 cut(s) 512
EcoRII CCWGG 3 cut(s) 10, 224, 478
EcoT14I CCWWGG 1 cut(s) 409
EcoT38I GRGCYC 1 cut(s) 458
ErhI CCWWGG 1 cut(s) 409
FaeI CATG 1 cut(s) 503
FalI AAGNNNNNCTT 2 cut(s) 336, 368
FatI CATG 1 cut(s) 499
FauI CCCGC 1 cut(s) 21
FauNDI CATATG 1 cut(s) 318
FbaI TGATCA 1 cut(s) 355
Fnu4HI GCNGC 4 cut(s) 79, 149, 152, 174
FriOI GRGCYC 1 cut(s) 458
Fsp4HI GCNGC 4 cut(s) 79, 149, 152, 174
GlaI GCGC 1 cut(s) 155
GluI GCNGC 4 cut(s) 79, 149, 152, 174
HaeII RGCGCY 1 cut(s) 157
HaeIII GGCC 1 cut(s) 513
HgaI GACGC 1 cut(s) 311
HhaI GCGC 1 cut(s) 156
Hin1II CATG 1 cut(s) 503
Hin6I GCGC 1 cut(s) 154
HinP1I GCGC 1 cut(s) 154
Hpy166II GTNNAC 2 cut(s) 106, 475
Hpy188I TCNGA 4 cut(s) 23, 211, 246, 544
Hpy188III TCNNGA 1 cut(s) 537
Hpy8I GTNNAC 2 cut(s) 106, 475
Hpy99I CGWCG 2 cut(s) 62, 65
HpyCH4III ACNGT 1 cut(s) 423
HpyCH4IV ACGT 1 cut(s) 57
HpyCH4V TGCA 5 cut(s) 257, 287, 428, 475, 503
HpyF10VI GCNNNNNNNGC 3 cut(s) 75, 157, 293
HpyF3I CTNAG 2 cut(s) 335, 457
HpySE526I ACGT 1 cut(s) 57
Hsp92II CATG 1 cut(s) 503
HspAI GCGC 1 cut(s) 154
Ksp22I TGATCA 1 cut(s) 355
Kzo9I GATC 5 cut(s) 136, 182, 355, 433, 539
Lsp1109I GCAGC 4 cut(s) 65, 160, 160, 163
LweI GCATC 1 cut(s) 349
MaeII ACGT 1 cut(s) 57
MaeIII GTNAC 1 cut(s) 270
MalI GATC 5 cut(s) 138, 184, 357, 435, 541
MbiI CCGCTC 1 cut(s) 235
MboI GATC 5 cut(s) 136, 182, 355, 433, 539
MboII GAAGA 2 cut(s) 28, 530
MflI RGATCY 2 cut(s) 182, 433
MhlI GDGCHC 2 cut(s) 458, 477
MluCI AATT 4 cut(s) 392, 416, 447, 504
MmeI TCCRAC 2 cut(s) 78, 305
MnlI CCTC 5 cut(s) 10, 54, 306, 330, 503
MseI TTAA 1 cut(s) 117
MspR9I CCNGG 3 cut(s) 12, 226, 480
MvaI CCWGG 3 cut(s) 12, 226, 480
MwoI GCNNNNNNNGC 3 cut(s) 75, 157, 293
NdeI CATATG 1 cut(s) 318
NdeII GATC 5 cut(s) 136, 182, 355, 433, 539
NlaIII CATG 1 cut(s) 503
NlaIV GGNNCC 2 cut(s) 52, 231
PcsI WCGNNNNNNNCGW 1 cut(s) 54
PkrI GCNGC 4 cut(s) 80, 150, 153, 175
Psp6I CCWGG 3 cut(s) 10, 224, 478
PspGI CCWGG 3 cut(s) 10, 224, 478
PspN4I GGNNCC 2 cut(s) 52, 231
PspPI GGNCC 1 cut(s) 512
PsuI RGATCY 2 cut(s) 182, 433
RsaI GTAC 1 cut(s) 33
RsaNI GTAC 1 cut(s) 32
SaqAI TTAA 1 cut(s) 117
SatI GCNGC 4 cut(s) 79, 149, 152, 174
Sau3AI GATC 5 cut(s) 136, 182, 355, 433, 539
Sau96I GGNCC 1 cut(s) 512
ScrFI CCNGG 3 cut(s) 12, 226, 480
SduI GDGCHC 2 cut(s) 458, 477
SetI ASST 5 cut(s) 21, 60, 103, 124, 279
SfaNI GCATC 1 cut(s) 349
Sse9I AATT 4 cut(s) 392, 416, 447, 504
SsiI CCGC 3 cut(s) 28, 67, 233
StyD4I CCNGG 3 cut(s) 10, 224, 478
StyI CCWWGG 1 cut(s) 409
TaaI ACNGT 1 cut(s) 423
TaiI ACGT 1 cut(s) 60
TaqI TCGA 1 cut(s) 508
TasI AATT 4 cut(s) 392, 416, 447, 504
Tru1I TTAA 1 cut(s) 117
Tru9I TTAA 1 cut(s) 117
TscAI CASTG 1 cut(s) 76
TseI GCWGC 4 cut(s) 78, 148, 151, 173
TspDTI ATGAA 3 cut(s) 263, 385, 458
TspGWI ACGGA 1 cut(s) 315
TspRI CASTG 1 cut(s) 76
VneI GTGCAC 1 cut(s) 473
XapI RAATTY 1 cut(s) 392
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.