pycom10g06380

HVA22-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0001583
Physical Location & Seq
Forward (+)
7371666 .. 7374479
2814 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g06380.5

Sequence Viewer

Length: 555 bp
ATGGCTCTACCAAGCGAGGTGGGATTGCGGGTACTGCTTTGTCCCTTTGGTTCCAACGTCCTTGTCCGGACCGCTTGCTGCTCTGTGGGGATTGCTTTACCTGTTTACTCGACGTTTAAAGCTATTGAGAAGAAGGATCAAGAGGAGCAACAAAGAATGCTTCTTTATTGGGCAGCTTATGGATCTTTCAGTGTTGCAGAAGTATTTTCAGACAAAATTCTCTCCTGGTTTCCGCTCTACTACCATATGAAGTTTGCATTTCTTGTTTGGTTACAACTTCCAGCCTCAAATGGAGCAAGGCATTTGTACATGAACCATCTGCGTCCATTCTTTCGGAGACATCAAGTCCGACTTGATCAAGCTGTGGATTTTATTTATGGCCAAATGACTAAATTCATCAGTTCACACCAAGGAGAATTACAGTTTGCAAAGGCACTTTTCATGAAGATTGTGGCTTCAGTTAGGGGCATCGTCCCAGGGCAAAGGCAATCAAATCGTGCGATCGAAGGCCCTGCGAAAGAGAGTCAAGACACTGAGTCAGATATAGATGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TB2_DP1_HVA22

Protein Analysis

185

Amino Acids

21.02

Weight (kDa)

8.77

Isoelectric Point (pI)

51.17

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 235
AccIII TCCGGA 1 cut(s) 66
AciI CCGC 3 cut(s) 28, 72, 233
AclWI GGATC 2 cut(s) 144, 190
AcoI YGGCCR 1 cut(s) 379
AcsI RAATTY 2 cut(s) 216, 392
AcuI CTGAAG 1 cut(s) 441
AfaI GTAC 2 cut(s) 33, 308
AhdI GACNNNNNGTC 2 cut(s) 344, 535
AjnI CCWGG 2 cut(s) 224, 475
AluBI AGCT 3 cut(s) 122, 176, 362
AluI AGCT 3 cut(s) 122, 176, 362
Alw26I GTCTC 1 cut(s) 331
AlwI GGATC 2 cut(s) 144, 190
Aor13HI TCCGGA 1 cut(s) 66
AoxI GGCC 2 cut(s) 379, 508
ApeKI GCWGC 2 cut(s) 78, 173
ApoI RAATTY 2 cut(s) 216, 392
AspS9I GGNCC 2 cut(s) 69, 509
AvaII GGWCC 1 cut(s) 69
BalI TGGCCA 1 cut(s) 381
BbvI GCAGC 2 cut(s) 65, 185
BccI CCATC 1 cut(s) 324
BcgI CGANNNNNNTGC 4 cut(s) 476, 494, 510, 528
BciT130I CCWGG 2 cut(s) 226, 477
BclI TGATCA 1 cut(s) 355
BcoDI GTCTC 1 cut(s) 331
BisI GCNGC 2 cut(s) 79, 174
BlsI GCNGC 2 cut(s) 80, 175
Bme1390I CCNGG 2 cut(s) 226, 477
Bme18I GGWCC 1 cut(s) 69
BmeRI GACNNNNNGTC 2 cut(s) 344, 535
BmgT120I GGNCC 2 cut(s) 69, 509
BmiI GGNNCC 1 cut(s) 52
BmrFI CCNGG 2 cut(s) 226, 477
BmsI GCATC 1 cut(s) 477
BsaJI CCNNGG 3 cut(s) 409, 475, 476
BsaWI WCCGGW 1 cut(s) 66
BseAI TCCGGA 1 cut(s) 66
BseBI CCWGG 2 cut(s) 226, 477
BseDI CCNNGG 3 cut(s) 409, 475, 476
BseMII CTCAG 1 cut(s) 525
BseRI GAGGAG 1 cut(s) 158
BseXI GCAGC 2 cut(s) 65, 185
Bsh1285I CGRYCG 1 cut(s) 504
BshFI GGCC 2 cut(s) 381, 510
BsiEI CGRYCG 1 cut(s) 504
BsiSI CCGG 1 cut(s) 67
BslFI GGGAC 2 cut(s) 27, 458
BsmAI GTCTC 1 cut(s) 331
BsmFI GGGAC 2 cut(s) 27, 458
BsmI GAATGC 1 cut(s) 162
BsnI GGCC 2 cut(s) 381, 510
Bsp13I TCCGGA 1 cut(s) 66
Bsp1407I TGTACA 1 cut(s) 306
Bsp143I GATC 4 cut(s) 136, 182, 355, 501
BspACI CCGC 3 cut(s) 28, 72, 233
BspANI GGCC 2 cut(s) 381, 510
BspCNI CTCAG 1 cut(s) 526
BspEI TCCGGA 1 cut(s) 66
BspHI TCATGA 1 cut(s) 441
BspLI GGNNCC 1 cut(s) 52
BspPI GGATC 2 cut(s) 144, 190
BsrBI CCGCTC 1 cut(s) 235
BsrGI TGTACA 1 cut(s) 306
BssECI CCNNGG 3 cut(s) 409, 475, 476
BssMI GATC 4 cut(s) 136, 182, 355, 501
BssT1I CCWWGG 1 cut(s) 409
Bst2UI CCWGG 2 cut(s) 226, 477
Bst4CI ACNGT 1 cut(s) 423
BstAUI TGTACA 1 cut(s) 306
BstC8I GCNNGC 1 cut(s) 76
BstDEI CTNAG 1 cut(s) 534
BstKTI GATC 4 cut(s) 139, 185, 358, 504
BstMAI GTCTC 1 cut(s) 331
BstMBI GATC 4 cut(s) 136, 182, 355, 501
BstMCI CGRYCG 1 cut(s) 504
BstMWI GCNNNNNNNGC 1 cut(s) 34
BstNI CCWGG 2 cut(s) 226, 477
BstSCI CCNGG 2 cut(s) 224, 475
BstV1I GCAGC 2 cut(s) 65, 185
BstX2I RGATCY 1 cut(s) 182
BstYI RGATCY 1 cut(s) 182
BsuRI GGCC 2 cut(s) 381, 510
BtsIMutI CAGTG 2 cut(s) 196, 531
Cac8I GCNNGC 1 cut(s) 76
CciI TCATGA 1 cut(s) 441
Cfr13I GGNCC 2 cut(s) 69, 509
CpoI CGGWCCG 1 cut(s) 69
CseI GACGC 1 cut(s) 311
Csp6I GTAC 2 cut(s) 32, 307
CspCI CAANNNNNGTGG 1 cut(s) 35
CspI CGGWCCG 1 cut(s) 69
CviAII CATG 2 cut(s) 310, 442
CviJI RGCY 8 cut(s) 5, 122, 176, 284, 362, 381, 455, 510
CviKI_1 RGCY 8 cut(s) 5, 122, 176, 284, 362, 381, 455, 510
CviQI GTAC 2 cut(s) 32, 307
DdeI CTNAG 1 cut(s) 534
DpnI GATC 4 cut(s) 138, 184, 357, 503
DpnII GATC 4 cut(s) 136, 182, 355, 501
DraI TTTAAA 1 cut(s) 118
DriI GACNNNNNGTC 2 cut(s) 344, 535
EaeI YGGCCR 1 cut(s) 379
Eam1105I GACNNNNNGTC 2 cut(s) 344, 535
Eco130I CCWWGG 1 cut(s) 409
Eco47I GGWCC 1 cut(s) 69
Eco57I CTGAAG 1 cut(s) 441
EcoO109I RGGNCCY 1 cut(s) 509
EcoRII CCWGG 2 cut(s) 224, 475
EcoT14I CCWWGG 1 cut(s) 409
ErhI CCWWGG 1 cut(s) 409
FaeI CATG 2 cut(s) 313, 445
FaiI YATR 7 cut(s) 180, 246, 248, 311, 378, 443, 545
FalI AAGNNNNNCTT 2 cut(s) 336, 368
FaqI GGGAC 2 cut(s) 27, 458
FatI CATG 2 cut(s) 309, 441
FauI CCCGC 1 cut(s) 21
FauNDI CATATG 1 cut(s) 246
FbaI TGATCA 1 cut(s) 355
Fnu4HI GCNGC 2 cut(s) 79, 174
Fsp4HI GCNGC 2 cut(s) 79, 174
GluI GCNGC 2 cut(s) 79, 174
HaeIII GGCC 2 cut(s) 381, 510
HapII CCGG 1 cut(s) 67
HgaI GACGC 1 cut(s) 311
Hin1II CATG 2 cut(s) 313, 445
HinfI GANTC 2 cut(s) 523, 536
HpaII CCGG 1 cut(s) 67
Hpy166II GTNNAC 2 cut(s) 106, 404
Hpy188I TCNGA 4 cut(s) 211, 336, 350, 541
Hpy188III TCNNGA 4 cut(s) 67, 140, 442, 527
Hpy8I GTNNAC 2 cut(s) 106, 404
Hpy99I CGWCG 1 cut(s) 115
HpyAV CCTTC 2 cut(s) 127, 500
HpyCH4III ACNGT 1 cut(s) 423
HpyCH4IV ACGT 2 cut(s) 57, 113
HpyCH4V TGCA 3 cut(s) 197, 257, 428
HpyF10VI GCNNNNNNNGC 1 cut(s) 34
HpyF3I CTNAG 1 cut(s) 534
HpySE526I ACGT 2 cut(s) 57, 113
Hsp92II CATG 2 cut(s) 313, 445
Kpn2I TCCGGA 1 cut(s) 66
Ksp22I TGATCA 1 cut(s) 355
Kzo9I GATC 4 cut(s) 136, 182, 355, 501
LmnI GCTCC 2 cut(s) 145, 293
LpnPI CCDG 8 cut(s) 80, 114, 211, 238, 294, 462, 489, 525
Lsp1109I GCAGC 2 cut(s) 65, 185
LweI GCATC 1 cut(s) 477
MaeII ACGT 2 cut(s) 57, 113
MaeIII GTNAC 1 cut(s) 270
MalI GATC 4 cut(s) 138, 184, 357, 503
MbiI CCGCTC 1 cut(s) 235
MboI GATC 4 cut(s) 136, 182, 355, 501
MboII GAAGA 2 cut(s) 142, 457
MflI RGATCY 1 cut(s) 182
MlsI TGGCCA 1 cut(s) 381
MluCI AATT 3 cut(s) 216, 392, 416
MluNI TGGCCA 1 cut(s) 381
MlyI GAGTC 2 cut(s) 532, 545
MmeI TCCRAC 2 cut(s) 78, 373
MnlI CCTC 3 cut(s) 10, 136, 295
Mox20I TGGCCA 1 cut(s) 381
MroI TCCGGA 1 cut(s) 66
MscI TGGCCA 1 cut(s) 381
MseI TTAA 1 cut(s) 117
Msp20I TGGCCA 1 cut(s) 381
MspI CCGG 1 cut(s) 67
MspR9I CCNGG 2 cut(s) 226, 477
Mva1269I GAATGC 1 cut(s) 162
MvaI CCWGG 2 cut(s) 226, 477
MwoI GCNNNNNNNGC 1 cut(s) 34
NdeI CATATG 1 cut(s) 246
NdeII GATC 4 cut(s) 136, 182, 355, 501
NlaIII CATG 2 cut(s) 313, 445
NlaIV GGNNCC 1 cut(s) 52
PagI TCATGA 1 cut(s) 441
PasI CCCWGGG 1 cut(s) 476
PctI GAATGC 1 cut(s) 162
PkrI GCNGC 2 cut(s) 80, 175
Ple19I CGATCG 1 cut(s) 504
PleI GAGTC 2 cut(s) 531, 544
PpsI GAGTC 2 cut(s) 531, 544
Psp6I CCWGG 2 cut(s) 224, 475
PspGI CCWGG 2 cut(s) 224, 475
PspN4I GGNNCC 1 cut(s) 52
PspPI GGNCC 2 cut(s) 69, 509
PsuI RGATCY 1 cut(s) 182
PvuI CGATCG 1 cut(s) 504
RsaI GTAC 2 cut(s) 33, 308
RsaNI GTAC 2 cut(s) 32, 307
Rsr2I CGGWCCG 1 cut(s) 69
RsrII CGGWCCG 1 cut(s) 69
SaqAI TTAA 1 cut(s) 117
SatI GCNGC 2 cut(s) 79, 174
Sau3AI GATC 4 cut(s) 136, 182, 355, 501
Sau96I GGNCC 2 cut(s) 69, 509
SchI GAGTC 2 cut(s) 532, 545
ScrFI CCNGG 2 cut(s) 226, 477
SetI ASST 7 cut(s) 21, 60, 103, 116, 124, 178, 364
SfaNI GCATC 1 cut(s) 477
SinI GGWCC 1 cut(s) 69
Sse9I AATT 3 cut(s) 216, 392, 416
SsiI CCGC 3 cut(s) 28, 72, 233
StyD4I CCNGG 2 cut(s) 224, 475
StyI CCWWGG 1 cut(s) 409
TaaI ACNGT 1 cut(s) 423
TaiI ACGT 2 cut(s) 60, 116
TaqI TCGA 2 cut(s) 110, 504
TasI AATT 3 cut(s) 216, 392, 416
TatI WGTACW 1 cut(s) 306
Tru1I TTAA 1 cut(s) 117
Tru9I TTAA 1 cut(s) 117
TscAI CASTG 2 cut(s) 196, 538
TseI GCWGC 2 cut(s) 78, 173
TspDTI ATGAA 5 cut(s) 263, 326, 385, 430, 458
TspRI CASTG 2 cut(s) 196, 538
VpaK11BI GGWCC 1 cut(s) 69
XapI RAATTY 2 cut(s) 216, 392
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.