Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb2

Orthogroup ID

OG0001724

Physical Location & Seq

Genomic Coordinates Forward (+)

18197021 .. 18201132

Gene Structure 4112 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_2g22070.t2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1374 bp

ATGGCGACAGATTTACTGCTGAGGCCGTCGTGCAGAGGTTGTGGTTCGACGTCGGAATTATACGGAAGCAACTGCAAGCACACCACCCTGTGCGTTTCGTGTGGCAAATCCATGGCTGAGGACAAGGCCAAATGCTCTGACTGTGGCGCCATTCTCACTCGCTTGATTCGAGAGTATAGTGTTTGGGTGAATACTGTTGATGAGAAGGAGTACTTCATTGGTAAATTCTCGACGGGGCTGCCAAAATTTTTGGAGAAGAAAAGTTGTGAAAATAAATGGTCTCTGTGTAAAAAGAAAACTAAAGGTGGATTACAAGGACGCCAAGATAGCTTTCAGGATAAGTTGCTCAGAAATAAACCATGGGTGTTGGAGGATGAAACAGGTCATACTCTGTACCAGGGAGATCCTGAACTGTCTGCAGCTTACTATCTACTAATGCGGAAAGGAAATAAGGAGATTTCCGCTATCCCTGCTGGTTCTTGGTTTAACTTTAAAAAGGTTGCACAGTATAAGCAATTTACTTTGGAGGAAGCTGAAGATAGAATGAGCAGTAGGAAAAAGAAGCAAGATGGACATGAAAGATGGATACTGAAAGCTGCAACTAATGGAGCTGTTGCATATGGTAATGTGGAGAAGTTTGGTGATAATGATAGTAAATACAAAATTGGTGATGATGATGAAGGTGTAAGTGGAAATGATGACTTTGATGATGATGATGATGATAGTGAGAAGGGCGATGACTGGGAGCATGATGAAATTTTCAGTGATGATGAAGAAAATACTCTTGAGGAAATAGAGGACTTCGCAACTGAGGTTCCAGCTCCAACAAAATTCAAGCAGGATGGTGATGAAGATTATAACAATGATGAAGATGGCAGACTAAGCAAATCCGGAAAAGAGTTAAAGAAGCTACTTGGCTGCCTGAACGAGTCTGATACAGAGGATGATGATGCCAATGGGGATGAGGATATGGATGATGTTATTGACAATGGTCCCTCAAAACTAGTGCCTTCTGGAACTTCTCAAACAACTCCATCTACCTCCAAGAGAAAGAGAAAAGGCAATACTGATGAATCCAAGGCATCTCCGCACAAGAAGGCGAAGACTGAAAATAATCAAAACCTTTCCGTCAACGAAGAGCCTGCATCTGCTGGTCCTGTCACTCAAGAAGAAATCCGGGCTGTTTTGATGCAACAGTCACCAATCACCATGCGTGATCTTCTTAATAACTTTGCAGGAAGACTCCGGTCAAAAGAGGAGAAGGAAGCTTTTACAGCAATCCTGAAGGACATGCGTTCTCAGAAAACGATTTCTAGGAGATCAAAAGATGATGGAACTGGTTGTTTTGTTGTACTGAGGGAGAAAATGAACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005515 GO:0008022

KEGG Pathways

Metabolic & Signaling

ko03022 map03022

Pfam Domains

Protein Families

TFIIF_alpha

Protein Analysis

458

Amino Acids

51.25

Weight (kDa)

4.95

Isoelectric Point (pI)

45.68

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TFIIF_alpha	PF05793	46 - 219	4.1e-30	Transcription initiation factor IIF, alpha subunit (TFIIF-alpha)
TFIIF_alpha	PF05793	222 - 437	7.6e-21	Transcription initiation factor IIF, alpha subunit (TFIIF-alpha)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001724)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G12610 AT4G12610
fragaria_vesca	FvH4_2g22070 FvH4_2g22070 FvH4_2g22070 FvH4_3g01042
malus_domestica	MD05G1357300.v1.1 MD10G1331000.v1.1
prunus_persica	Prupe.4G011300_v2.0.a1 Prupe.4G011300_v2.0.a1
pyrus_communis	pycom05g32330 pycom10g28050
rosa_chinensis	RchiOBHm_Chr5g0001581 RchiOBHm_Chr6g0288941
rosa_laevigata	RLG00000009133 RLG00000012342 RLG00000012344 RLG00000030944
rosa_multiflora	Rmu_sc0003749.1_g000001 Rmu_sc0006320.1_g000007 Rmu_sc0007182.1_g000001
rosa_roxburghii	Rroxscaffold_1G00074740 Rroxscaffold_7G00179460 Rroxscaffold_7G00179470 Rroxscaffold_7G00179490
rosa_rugosa	Rorug04G0391100 Rorug06G0199700 Rorug06G0201700
rosa_samantha	Rh2BG455300 Rh2DG465300 Rh5AG012500 Rh5BG015400 Rh5CG013500 Rh5CG313700 Rh5DG013300 Rh6AG312800 Rh6BG317400 Rh6BG319600 Rh6CG326300 Rh6DG312400
rosa_wichuraiana	Rw0G003180 Rw0G005580 Rw1G016380 Rw2G040560 Rw5G001200 Rw6G027030 Rw7G019510 Rw7G019540

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	858
AatII	GACGTC	1 cut(s)	53
AccB1I	GGYRCC	1 cut(s)	146
AccIII	TCCGGA	1 cut(s)	890
AciI	CCGC	3 cut(s)	439, 462, 1088
AclWI	GGATC	1 cut(s)	398
AcsI	RAATTY	4 cut(s)	224, 245, 756, 830
AcuI	CTGAAG	2 cut(s)	555, 1304
AcyI	GRCGYC	3 cut(s)	50, 147, 319
AdeI	CACNNNGTG	1 cut(s)	90
AfaI	GTAC	3 cut(s)	212, 395, 1353
AgsI	TTSAA	1 cut(s)	835
AhlI	ACTAGT	1 cut(s)	1003
AjnI	CCWGG	1 cut(s)	396
AjuI	GAANNNNNNNTTGG	2 cut(s)	315, 347
AluBI	AGCT	8 cut(s)	330, 422, 533, 596, 611, 821, 910, 1268
AluI	AGCT	8 cut(s)	330, 422, 533, 596, 611, 821, 910, 1268
Alw26I	GTCTC	1 cut(s)	285
AlwI	GGATC	1 cut(s)	398
Aor13HI	TCCGGA	1 cut(s)	890
AoxI	GGCC	2 cut(s)	23, 126
ApeKI	GCWGC	4 cut(s)	238, 419, 596, 918
ApoI	RAATTY	4 cut(s)	224, 245, 756, 830
AspLEI	GCGC	1 cut(s)	149
AspS9I	GGNCC	2 cut(s)	992, 1154
AsuC2I	CCSGG	1 cut(s)	1178
AsuHPI	GGTGA	6 cut(s)	199, 653, 680, 857, 1191, 1198
AvaII	GGWCC	2 cut(s)	992, 1154
BanI	GGYRCC	1 cut(s)	146
BbsI	GAAGAC	2 cut(s)	1109, 1246
BbvCI	CCTCAGC	2 cut(s)	20, 117
BbvI	GCAGC	4 cut(s)	225, 431, 583, 905
BccI	CCATC	6 cut(s)	563, 576, 836, 866, 1042, 1325
BceAI	ACGGC	1 cut(s)	10
BcgI	CGANNNNNNTGC	4 cut(s)	220, 254, 1124, 1158
BciT130I	CCWGG	1 cut(s)	398
BciVI	GTATCC	1 cut(s)	579
BcnI	CCSGG	1 cut(s)	1178
BcoDI	GTCTC	1 cut(s)	285
BcuI	ACTAGT	1 cut(s)	1003
BfaI	CTAG	2 cut(s)	1004, 1314
BfmI	CTRYAG	1 cut(s)	417
BfoI	RGCGCY	1 cut(s)	150
BfuI	GTATCC	1 cut(s)	579
BisI	GCNGC	4 cut(s)	239, 420, 597, 919
BlsI	GCNGC	4 cut(s)	240, 421, 598, 920
BmcAI	AGTACT	1 cut(s)	212
Bme1390I	CCNGG	2 cut(s)	398, 1178
Bme18I	GGWCC	2 cut(s)	992, 1154
BmgT120I	GGNCC	2 cut(s)	992, 1154
BmiI	GGNNCC	3 cut(s)	148, 816, 994
BmrFI	CCNGG	2 cut(s)	398, 1178
BmrI	ACTGGG	1 cut(s)	751
BmsI	GCATC	4 cut(s)	940, 1091, 1154, 1179
BmuI	ACTGGG	1 cut(s)	751
BoxI	GACNNNNGTC	2 cut(s)	990, 1246
BpiI	GAAGAC	2 cut(s)	1109, 1246
Bpu10I	CCTNAGC	2 cut(s)	20, 117
BpuEI	CTTGAG	2 cut(s)	806, 1149
BpuMI	CCSGG	1 cut(s)	1178
BsaHI	GRCGYC	3 cut(s)	50, 147, 319
BsaI	GGTCTC	1 cut(s)	285
BsaJI	CCNNGG	4 cut(s)	111, 359, 397, 1077
BsaWI	WCCGGW	2 cut(s)	890, 1245
Bse1I	ACTGG	2 cut(s)	746, 1342
BseAI	TCCGGA	1 cut(s)	890
BseBI	CCWGG	1 cut(s)	398
BseDI	CCNNGG	4 cut(s)	111, 359, 397, 1077
BseGI	GGATG	5 cut(s)	379, 847, 949, 967, 979
BseMII	CTCAG	6 cut(s)	11, 108, 361, 801, 1313, 1346
BseNI	ACTGG	2 cut(s)	746, 1342
BseRI	GAGGAG	1 cut(s)	1271
BseXI	GCAGC	4 cut(s)	225, 431, 583, 905
BsgI	GTGCAG	1 cut(s)	52
BshFI	GGCC	2 cut(s)	25, 128
BshNI	GGYRCC	1 cut(s)	146
BsiSI	CCGG	3 cut(s)	891, 1177, 1246
BslFI	GGGAC	1 cut(s)	978
BsmAI	GTCTC	1 cut(s)	285
BsmFI	GGGAC	1 cut(s)	978
BsnI	GGCC	2 cut(s)	25, 128
Bso31I	GGTCTC	1 cut(s)	285
Bsp13I	TCCGGA	1 cut(s)	890
Bsp143I	GATC	3 cut(s)	403, 1216, 1319
Bsp19I	CCATGG	2 cut(s)	111, 359
BspACI	CCGC	3 cut(s)	439, 462, 1088
BspANI	GGCC	2 cut(s)	25, 128
BspCNI	CTCAG	6 cut(s)	12, 109, 360, 802, 1312, 1347
BspEI	TCCGGA	1 cut(s)	890
BspLI	GGNNCC	3 cut(s)	148, 816, 994
BspMAI	CTGCAG	1 cut(s)	421
BspPI	GGATC	1 cut(s)	398
BspQI	GCTCTTC	1 cut(s)	1131
BspT107I	GGYRCC	1 cut(s)	146
BspTNI	GGTCTC	1 cut(s)	285
BsrI	ACTGG	2 cut(s)	746, 1342
BssECI	CCNNGG	4 cut(s)	111, 359, 397, 1077
BssMI	GATC	3 cut(s)	403, 1216, 1319
BssNI	GRCGYC	3 cut(s)	50, 147, 319
BssT1I	CCWWGG	3 cut(s)	111, 359, 1077
Bst2UI	CCWGG	1 cut(s)	398
Bst4CI	ACNGT	5 cut(s)	143, 196, 414, 507, 1197
Bst6I	CTCTTC	1 cut(s)	1131
BstACI	GRCGYC	3 cut(s)	50, 147, 319
BstC8I	GCNNGC	2 cut(s)	77, 1143
BstDEI	CTNAG	7 cut(s)	20, 117, 347, 810, 881, 1299, 1355
BstDSI	CCRYGG	2 cut(s)	111, 359
BstF5I	GGATG	5 cut(s)	379, 847, 949, 967, 979
BstH2I	RGCGCY	1 cut(s)	150
BstHHI	GCGC	1 cut(s)	149
BstKTI	GATC	3 cut(s)	406, 1219, 1322
BstMAI	GTCTC	1 cut(s)	285
BstMBI	GATC	3 cut(s)	403, 1216, 1319
BstMWI	GCNNNNNNNGC	4 cut(s)	327, 470, 882, 1274
BstNI	CCWGG	1 cut(s)	398
BstNSI	RCATGY	1 cut(s)	1294
BstPAI	GACNNNNGTC	2 cut(s)	990, 1246
BstSCI	CCNGG	2 cut(s)	396, 1176
BstSFI	CTRYAG	1 cut(s)	417
BstV1I	GCAGC	4 cut(s)	225, 431, 583, 905
BstV2I	GAAGAC	2 cut(s)	1109, 1246
BstX2I	RGATCY	1 cut(s)	403
BstYI	RGATCY	1 cut(s)	403
BsuI	GTATCC	1 cut(s)	579
BsuRI	GGCC	2 cut(s)	25, 128
BtgI	CCRYGG	2 cut(s)	111, 359
BtgZI	GCGATG	1 cut(s)	750
BtsCI	GGATG	5 cut(s)	379, 847, 949, 967, 979
BtsIMutI	CAGTG	1 cut(s)	769
Cac8I	GCNNGC	2 cut(s)	77, 1143
CfoI	GCGC	1 cut(s)	149
Cfr13I	GGNCC	2 cut(s)	992, 1154
CseI	GACGC	1 cut(s)	327
Csp6I	GTAC	3 cut(s)	211, 394, 1352
CviAII	CATG	6 cut(s)	112, 360, 575, 749, 1210, 1291
CviQI	GTAC	3 cut(s)	211, 394, 1352
DdeI	CTNAG	7 cut(s)	20, 117, 347, 810, 881, 1299, 1355
DinI	GGCGCC	1 cut(s)	148
DpnI	GATC	3 cut(s)	405, 1218, 1321
DpnII	GATC	3 cut(s)	403, 1216, 1319
DraI	TTTAAA	1 cut(s)	493
DraIII	CACNNNGTG	1 cut(s)	90
Eam1104I	CTCTTC	1 cut(s)	1131
EarI	CTCTTC	1 cut(s)	1131
Eco130I	CCWWGG	3 cut(s)	111, 359, 1077
Eco31I	GGTCTC	1 cut(s)	285
Eco47I	GGWCC	2 cut(s)	992, 1154
Eco57I	CTGAAG	2 cut(s)	555, 1304
EcoRII	CCWGG	1 cut(s)	396
EcoT14I	CCWWGG	3 cut(s)	111, 359, 1077
EgeI	GGCGCC	1 cut(s)	148
EheI	GGCGCC	1 cut(s)	148
ErhI	CCWWGG	3 cut(s)	111, 359, 1077
FaeI	CATG	6 cut(s)	115, 363, 578, 752, 1213, 1294
FalI	AAGNNNNNCTT	2 cut(s)	197, 229
FaqI	GGGAC	1 cut(s)	978
FatI	CATG	6 cut(s)	111, 359, 574, 748, 1209, 1290
FauNDI	CATATG	1 cut(s)	619
Fnu4HI	GCNGC	4 cut(s)	239, 420, 597, 919
FokI	GGATG	5 cut(s)	386, 854, 956, 974, 986
Fsp4HI	GCNGC	4 cut(s)	239, 420, 597, 919
FspBI	CTAG	2 cut(s)	1004, 1314
GlaI	GCGC	1 cut(s)	148
GluI	GCNGC	4 cut(s)	239, 420, 597, 919
HaeII	RGCGCY	1 cut(s)	150
HaeIII	GGCC	2 cut(s)	25, 128
HapII	CCGG	3 cut(s)	891, 1177, 1246
HgaI	GACGC	1 cut(s)	327
HhaI	GCGC	1 cut(s)	149
Hin1I	GRCGYC	3 cut(s)	50, 147, 319
Hin1II	CATG	6 cut(s)	115, 363, 578, 752, 1213, 1294
Hin6I	GCGC	1 cut(s)	147
HinP1I	GCGC	1 cut(s)	147
HincII	GTYRAC	1 cut(s)	1132
HindII	GTYRAC	1 cut(s)	1132
HindIII	AAGCTT	1 cut(s)	1266
HinfI	GANTC	4 cut(s)	166, 929, 1073, 1242
HpaII	CCGG	3 cut(s)	891, 1177, 1246
HphI	GGTGA	6 cut(s)	199, 653, 680, 857, 1191, 1198
Hpy166II	GTNNAC	1 cut(s)	1132
Hpy188I	TCNGA	5 cut(s)	55, 139, 350, 934, 1302
Hpy188III	TCNNGA	9 cut(s)	170, 229, 335, 407, 785, 891, 1014, 1166, 1282
Hpy8I	GTNNAC	1 cut(s)	1132
Hpy99I	CGWCG	4 cut(s)	31, 52, 55, 235
HpyAV	CCTTC	7 cut(s)	199, 674, 724, 1020, 1090, 1255, 1279
HpyCH4III	ACNGT	5 cut(s)	143, 196, 414, 507, 1197
HpyCH4IV	ACGT	1 cut(s)	50
HpyCH4V	TGCA	9 cut(s)	33, 75, 419, 503, 599, 617, 1145, 1192, 1235
HpyF10VI	GCNNNNNNNGC	4 cut(s)	327, 470, 882, 1274
HpyF3I	CTNAG	7 cut(s)	20, 117, 347, 810, 881, 1299, 1355
HpySE526I	ACGT	1 cut(s)	50
Hsp92I	GRCGYC	3 cut(s)	50, 147, 319
Hsp92II	CATG	6 cut(s)	115, 363, 578, 752, 1213, 1294
HspAI	GCGC	1 cut(s)	147
KasI	GGCGCC	1 cut(s)	146
Kpn2I	TCCGGA	1 cut(s)	890
Kzo9I	GATC	3 cut(s)	403, 1216, 1319
LguI	GCTCTTC	1 cut(s)	1131
LmnI	GCTCC	3 cut(s)	608, 745, 826
Lsp1109I	GCAGC	4 cut(s)	225, 431, 583, 905
LweI	GCATC	4 cut(s)	940, 1091, 1154, 1179
MaeI	CTAG	2 cut(s)	1004, 1314
MaeII	ACGT	1 cut(s)	50
MaeIII	GTNAC	2 cut(s)	1159, 1197
MalI	GATC	3 cut(s)	405, 1218, 1321
MboI	GATC	3 cut(s)	403, 1216, 1319
MflI	RGATCY	1 cut(s)	403
MluCI	AATT	7 cut(s)	56, 224, 245, 515, 663, 756, 830
Mly113I	GGCGCC	1 cut(s)	147
MlyI	GAGTC	2 cut(s)	938, 1236
MmeI	TCCRAC	3 cut(s)	33, 348, 848
MroI	TCCGGA	1 cut(s)	890
MseI	TTAA	4 cut(s)	486, 492, 902, 1224
MspI	CCGG	3 cut(s)	891, 1177, 1246
MspR9I	CCNGG	2 cut(s)	398, 1178
MvaI	CCWGG	1 cut(s)	398
MwoI	GCNNNNNNNGC	4 cut(s)	327, 470, 882, 1274
NarI	GGCGCC	1 cut(s)	147
NciI	CCSGG	1 cut(s)	1178
NcoI	CCATGG	2 cut(s)	111, 359
NdeI	CATATG	1 cut(s)	619
NdeII	GATC	3 cut(s)	403, 1216, 1319
NlaIII	CATG	6 cut(s)	115, 363, 578, 752, 1213, 1294
NlaIV	GGNNCC	3 cut(s)	148, 816, 994
NmuCI	GTSAC	2 cut(s)	1159, 1197
NspI	RCATGY	1 cut(s)	1294
PciSI	GCTCTTC	1 cut(s)	1131
PcsI	WCGNNNNNNNCGW	1 cut(s)	166
PfeI	GAWTC	2 cut(s)	166, 1073
PkrI	GCNGC	4 cut(s)	240, 421, 598, 920
PleI	GAGTC	2 cut(s)	937, 1236
PluTI	GGCGCC	1 cut(s)	150
PpsI	GAGTC	2 cut(s)	937, 1236
PshAI	GACNNNNGTC	2 cut(s)	990, 1246
PsiI	TTATAA	1 cut(s)	858
Psp6I	CCWGG	1 cut(s)	396
PspGI	CCWGG	1 cut(s)	396
PspN4I	GGNNCC	3 cut(s)	148, 816, 994
PspPI	GGNCC	2 cut(s)	992, 1154
PstI	CTGCAG	1 cut(s)	421
PsuI	RGATCY	1 cut(s)	403
RsaI	GTAC	3 cut(s)	212, 395, 1353
RsaNI	GTAC	3 cut(s)	211, 394, 1352
SapI	GCTCTTC	1 cut(s)	1131
SaqAI	TTAA	4 cut(s)	486, 492, 902, 1224
SatI	GCNGC	4 cut(s)	239, 420, 597, 919
Sau3AI	GATC	3 cut(s)	403, 1216, 1319
Sau96I	GGNCC	2 cut(s)	992, 1154
ScaI	AGTACT	1 cut(s)	212
SchI	GAGTC	2 cut(s)	938, 1236
ScrFI	CCNGG	2 cut(s)	398, 1178
SfaNI	GCATC	4 cut(s)	940, 1091, 1154, 1179
SfcI	CTRYAG	1 cut(s)	417
SfoI	GGCGCC	1 cut(s)	148
SinI	GGWCC	2 cut(s)	992, 1154
SmlI	CTYRAG	2 cut(s)	785, 1164
SmoI	CTYRAG	2 cut(s)	785, 1164
SpeI	ACTAGT	1 cut(s)	1003
Sse9I	AATT	7 cut(s)	56, 224, 245, 515, 663, 756, 830
SsiI	CCGC	3 cut(s)	439, 462, 1088
SspDI	GGCGCC	1 cut(s)	146
SspMI	CTAG	2 cut(s)	1004, 1314
StyD4I	CCNGG	2 cut(s)	396, 1176
StyI	CCWWGG	3 cut(s)	111, 359, 1077
TaaI	ACNGT	5 cut(s)	143, 196, 414, 507, 1197
TaiI	ACGT	1 cut(s)	53
TaqI	TCGA	3 cut(s)	47, 169, 230
TasI	AATT	7 cut(s)	56, 224, 245, 515, 663, 756, 830
TatI	WGTACW	2 cut(s)	210, 1351
TfiI	GAWTC	2 cut(s)	166, 1073
Tru1I	TTAA	4 cut(s)	486, 492, 902, 1224
Tru9I	TTAA	4 cut(s)	486, 492, 902, 1224
TscAI	CASTG	1 cut(s)	769
TseFI	GTSAC	2 cut(s)	1159, 1197
TseI	GCWGC	4 cut(s)	238, 419, 596, 918
Tsp45I	GTSAC	2 cut(s)	1159, 1197
TspDTI	ATGAA	9 cut(s)	205, 390, 591, 693, 768, 786, 864, 882, 1086
TspGWI	ACGGA	2 cut(s)	78, 1117
TspRI	CASTG	1 cut(s)	769
VpaK11BI	GGWCC	2 cut(s)	992, 1154
XapI	RAATTY	4 cut(s)	224, 245, 756, 830
XceI	RCATGY	1 cut(s)	1294
XspI	CTAG	2 cut(s)	1004, 1314
ZraI	GACGTC	1 cut(s)	51
ZrmI	AGTACT	1 cut(s)	212

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.