Rroxscaffold_1G00074740

Transcription initiation factor IIF subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0001724
Physical Location & Seq
Reverse (-)
95616472 .. 95621521
5050 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00074740.1

Sequence Viewer

Length: 1662 bp
ATGTCGTCGGACTTGTTGCTGAAGCCGTCGTGCGGCGGATGCGGATCGACTTCTGATTTGTACGGGAGCAACTGTAAGCACATGACGCTGTGCTGTGCTTGCGGCAAAACCATGGCGGAGAACAAAGCCAAATGCTACGACTGCGGCGCCGTCGTCACTCGCTTGATTCGAGAATATAATGTGAGGGCAAGCACTGCCAGCGAGAAGAGCTACTTCATTGGTAGGTTTGTGACGGGGTTGCCCAACTTTTCGAAGAAGAAGAGTGCCGAAAATAAATGGACTCTTCGCAAGGATCTTTCGGATCCAAGAAGGAGTAAGGATAAGGATGGACAAGACCAGAAGAAATATAAGGATGCTCGCCCACCTACTGAGGCCTCTCGGGAGAAGCACAAAAACAAACCATGGGTCTTGGAGGACGAAACTGGCCAGTCTCAGTACAACGGTCAACTTGAAGGTGCACAATCAGCAATTTACTACCTACTAATGTTGCAAGGAAAGGAGTTTTCTGCTATTCCTGCTGGGTCGTGGTTCAACTTTAACAAGGTTGCACAATATAAGCAACTTACTCTAGAGGAAGCAGAAGAGAAGATGAACAATAGGAAAAAGACTCAAGATGGATATGAAAGATGGATGATGAAATCTGGAACTAACGAACCTGGTCCACTTCGTGAGAAGGAAACTGGTGTGGCTGGTGGTAAGGGACGCAAAAAAATGACTGGTGATGATGATGAGGGAAATTTCTCTGATAAAGGGGAGGAAGATGAGGAAGAAGAGACAGCAAGGAAAAATAGACTTGGTCTCAATAAAAAAGGTGGTGATGATGAGGATGATGAAGGTGGAAATGAAGAAGATGACTTAGATGATGATAGCAGTGAGAAGGGTGATGATTGGGAGCACGAAGAAATATTCACCGATGATGATGAAGCTGTTGGTATTAATCCTGAGGAGAGAGAAGAATTCGAACCTGAGGTTCCAGCTCCTCCAGAAATTAAGCAGGATGATGACGATGATGATAATAATGAAGAGGAGGGTGGGCTCAGCAAATCAGGAAAAGAGTTGAAGAAGCTACTTGGGCGAAGTGGTGGCCTGAATGATTCAGATGCAGAGGACGATGACGATGACGACGATGATGTAGGTTATATGGATGATGATATTGCCTTGCCTCAAGCGCTGGCACCAAAGCAGAAGGATGCACCCAAACAGGAACCAGTTGACAACAGTCCTATTAAACCCGGGACTTCTGGAACTGCTGCTAAAGCAAACCCATCTGCCTCCAAGTCGGCGAAGGGAAAGAGAAAATTAAATGGTGATGACGCTAAAGCATCCAACAGTGCACCTCCCAAGAAGGTGAAGACCGAAAATGAGCAAAAATCAGTCAAAGAGGAGCCTGCTTCTGAAAGTAGTGTGCCTGCAAAAGGTACTGCTGCATCCTCGAAAACGGGTTCAACACCATCTGGTGGTCCTACAGGTCCTGTATCTGAGGAAGAAATTAGGGCAGTTTTGATGCAAAAGTCACCCATCACCACACGCGATCTTGTTAATAACTTCCCCGGACGACTCAAATCCAAAGAGGAGAAGGATGCTTTTGCAGCAATCCTGAGGAAAATGTCTAGGATACAGAAGAACAACGGGATCAGCTATGTTGTATTGAGGGAGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005515 GO:0008022
KEGG Pathways
Metabolic & Signaling
ko03022 map03022
Pfam Domains
Protein Families
TFIIF_alpha

Protein Analysis

553

Amino Acids

60.75

Weight (kDa)

4.9

Isoelectric Point (pI)

50.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TFIIF_alpha PF05793 48 - 551 8.7e-120 Transcription initiation factor IIF, alpha subunit (TFIIF-alpha)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 146, 1174
AccB7I CCANNNNNTGG 1 cut(s) 1457
AccII CGCG 1 cut(s) 1530
AciI CCGC 6 cut(s) 33, 36, 42, 102, 116, 144
AclWI GGATC 5 cut(s) 52, 296, 300, 309, 1640
AcoI YGGCCR 1 cut(s) 424
AcsI RAATTY 2 cut(s) 736, 956
AcuI CTGAAG 1 cut(s) 41
AcyI GRCGYC 1 cut(s) 147
AdeI CACNNNGTG 1 cut(s) 668
AfaI GTAC 3 cut(s) 62, 437, 1420
AfeI AGCGCT 1 cut(s) 1170
AfiI CCNNNNNNNGG 3 cut(s) 32, 1415, 1457
AgsI TTSAA 4 cut(s) 452, 532, 1060, 1446
AjnI CCWGG 1 cut(s) 655
AluBI AGCT 5 cut(s) 210, 926, 977, 1066, 1638
AluI AGCT 5 cut(s) 210, 926, 977, 1066, 1638
Alw21I GWGCWC 3 cut(s) 460, 897, 1336
Alw26I GTCTC 3 cut(s) 435, 767, 803
Alw44I GTGCAC 2 cut(s) 456, 1332
AlwI GGATC 5 cut(s) 52, 296, 300, 309, 1640
AlwNI CAGNNNCTG 1 cut(s) 1472
Ama87I CYCGRG 2 cut(s) 378, 1232
Aor51HI AGCGCT 1 cut(s) 1170
AoxI GGCC 3 cut(s) 372, 424, 1084
ApaLI GTGCAC 2 cut(s) 456, 1332
ApeKI GCWGC 3 cut(s) 1250, 1424, 1589
ApoI RAATTY 2 cut(s) 736, 956
ArsI GACNNNNNNTTYG 4 cut(s) 40, 72, 390, 422
AseI ATTAAT 1 cut(s) 936
AspLEI GCGC 2 cut(s) 149, 1171
AspS9I GGNCC 3 cut(s) 659, 1460, 1469
AsuC2I CCSGG 3 cut(s) 1233, 1234, 1551
AsuHPI GGTGA 8 cut(s) 731, 827, 893, 901, 1319, 1360, 1506, 1513
AsuII TTCGAA 2 cut(s) 251, 960
AvaI CYCGRG 2 cut(s) 378, 1232
AvaII GGWCC 3 cut(s) 659, 1460, 1469
AxyI CCTNAGG 3 cut(s) 942, 966, 1598
BaeGI GKGCMC 2 cut(s) 460, 1336
BalI TGGCCA 1 cut(s) 426
BamHI GGATCC 1 cut(s) 301
BanI GGYRCC 2 cut(s) 146, 1174
BanII GRGCYC 1 cut(s) 1038
BbsI GAAGAC 1 cut(s) 1358
Bbv12I GWGCWC 3 cut(s) 460, 897, 1336
BbvI GCAGC 3 cut(s) 1237, 1411, 1601
BccI CCATC 6 cut(s) 320, 608, 621, 1273, 1459, 1526
BceAI ACGGC 2 cut(s) 10, 134
BciT130I CCWGG 1 cut(s) 657
BciVI GTATCC 1 cut(s) 1608
BcnI CCSGG 3 cut(s) 1233, 1234, 1551
BcoDI GTCTC 3 cut(s) 435, 767, 803
BfaI CTAG 2 cut(s) 569, 1611
BfmI CTRYAG 1 cut(s) 1464
BfoI RGCGCY 2 cut(s) 150, 1172
BfuI GTATCC 1 cut(s) 1608
BisI GCNGC 6 cut(s) 34, 103, 145, 1251, 1425, 1590
BlpI GCTNAGC 1 cut(s) 1037
BlsI GCNGC 6 cut(s) 35, 104, 146, 1252, 1426, 1591
Bme1390I CCNGG 4 cut(s) 657, 1233, 1234, 1551
Bme18I GGWCC 3 cut(s) 659, 1460, 1469
BmeT110I CYCGRG 2 cut(s) 378, 1232
BmgT120I GGNCC 3 cut(s) 659, 1460, 1469
BmiI GGNNCC 6 cut(s) 148, 303, 972, 1176, 1206, 1386
BmrFI CCNGG 4 cut(s) 657, 1233, 1234, 1551
BmsI GCATC 8 cut(s) 29, 343, 1090, 1180, 1331, 1436, 1494, 1570
BoxI GACNNNNGTC 1 cut(s) 1218
BpiI GAAGAC 1 cut(s) 1358
BpmI CTGGAG 1 cut(s) 966
Bpu1102I GCTNAGC 1 cut(s) 1037
Bpu14I TTCGAA 2 cut(s) 251, 960
BpuEI CTTGAG 2 cut(s) 594, 1149
BpuMI CCSGG 3 cut(s) 1233, 1234, 1551
BsaBI GATNNNNATC 1 cut(s) 43
BsaHI GRCGYC 1 cut(s) 147
BsaI GGTCTC 1 cut(s) 803
BsaJI CCNNGG 4 cut(s) 111, 401, 1232, 1549
BsaXI ACNNNNNCTCC 2 cut(s) 58, 88
Bsc4I CCNNNNNNNGG 3 cut(s) 32, 1415, 1457
Bse1I ACTGG 5 cut(s) 427, 427, 685, 721, 1208
Bse21I CCTNAGG 3 cut(s) 942, 966, 1598
Bse8I GATNNNNATC 1 cut(s) 43
BseBI CCWGG 1 cut(s) 657
BseDI CCNNGG 4 cut(s) 111, 401, 1232, 1549
BseJI GATNNNNATC 1 cut(s) 43
BseLI CCNNNNNNNGG 3 cut(s) 32, 1415, 1457
BseMII CTCAG 7 cut(s) 360, 446, 933, 957, 1051, 1470, 1589
BseNI ACTGG 5 cut(s) 427, 427, 685, 721, 1208
BseRI GAGGAG 5 cut(s) 959, 969, 1040, 1397, 1586
BseSI GKGCMC 2 cut(s) 460, 1336
BseXI GCAGC 3 cut(s) 1237, 1411, 1601
BseYI CCCAGC 1 cut(s) 518
Bsh1236I CGCG 1 cut(s) 1530
BshFI GGCC 3 cut(s) 374, 426, 1086
BshNI GGYRCC 2 cut(s) 146, 1174
BsiHKAI GWGCWC 3 cut(s) 460, 897, 1336
BsiHKCI CYCGRG 2 cut(s) 378, 1232
BsiSI CCGG 2 cut(s) 1233, 1551
BslFI GGGAC 2 cut(s) 714, 1249
BslI CCNNNNNNNGG 3 cut(s) 32, 1415, 1457
BsmAI GTCTC 3 cut(s) 435, 767, 803
BsmFI GGGAC 2 cut(s) 714, 1249
BsnI GGCC 3 cut(s) 374, 426, 1086
Bso31I GGTCTC 1 cut(s) 803
BsoBI CYCGRG 2 cut(s) 378, 1232
Bsp119I TTCGAA 2 cut(s) 251, 960
Bsp1286I GDGCHC 4 cut(s) 460, 897, 1038, 1336
Bsp143I GATC 5 cut(s) 44, 292, 301, 1531, 1632
Bsp1720I GCTNAGC 1 cut(s) 1037
Bsp19I CCATGG 2 cut(s) 111, 401
BspACI CCGC 6 cut(s) 33, 36, 42, 102, 116, 144
BspANI GGCC 3 cut(s) 374, 426, 1086
BspCNI CTCAG 7 cut(s) 361, 445, 934, 958, 1050, 1471, 1590
BspFNI CGCG 1 cut(s) 1530
BspLI GGNNCC 6 cut(s) 148, 303, 972, 1176, 1206, 1386
BspPI GGATC 5 cut(s) 52, 296, 300, 309, 1640
BspQI GCTCTTC 1 cut(s) 200
BspT104I TTCGAA 2 cut(s) 251, 960
BspT107I GGYRCC 2 cut(s) 146, 1174
BspTNI GGTCTC 1 cut(s) 803
BsrI ACTGG 5 cut(s) 427, 427, 685, 721, 1208
BssECI CCNNGG 4 cut(s) 111, 401, 1232, 1549
BssMI GATC 5 cut(s) 44, 292, 301, 1531, 1632
BssNI GRCGYC 1 cut(s) 147
BssT1I CCWWGG 2 cut(s) 111, 401
Bst2UI CCWGG 1 cut(s) 657
Bst4CI ACNGT 4 cut(s) 74, 443, 1220, 1331
Bst6I CTCTTC 6 cut(s) 200, 254, 288, 576, 765, 1017
BstACI GRCGYC 1 cut(s) 147
BstAPI GCANNNNNTGC 1 cut(s) 194
BstBI TTCGAA 2 cut(s) 251, 960
BstC8I GCNNGC 7 cut(s) 100, 190, 199, 358, 1173, 1389, 1410
BstDEI CTNAG 8 cut(s) 369, 432, 856, 942, 966, 1037, 1479, 1598
BstDSI CCRYGG 2 cut(s) 111, 401
BstENI CCTNNNNNAGG 1 cut(s) 1413
BstFNI CGCG 1 cut(s) 1530
BstH2I RGCGCY 2 cut(s) 150, 1172
BstHHI GCGC 2 cut(s) 149, 1171
BstKTI GATC 5 cut(s) 47, 295, 304, 1534, 1635
BstMAI GTCTC 3 cut(s) 435, 767, 803
BstMBI GATC 5 cut(s) 44, 292, 301, 1531, 1632
BstNI CCWGG 1 cut(s) 657
BstPAI GACNNNNGTC 1 cut(s) 1218
BstSCI CCNGG 4 cut(s) 655, 1231, 1232, 1549
BstSFI CTRYAG 1 cut(s) 1464
BstSLI GKGCMC 2 cut(s) 460, 1336
BstUI CGCG 1 cut(s) 1530
BstV1I GCAGC 3 cut(s) 1237, 1411, 1601
BstV2I GAAGAC 1 cut(s) 1358
BstX2I RGATCY 2 cut(s) 292, 301
BstYI RGATCY 2 cut(s) 292, 301
Bsu36I CCTNAGG 3 cut(s) 942, 966, 1598
BsuI GTATCC 1 cut(s) 1608
BsuRI GGCC 3 cut(s) 374, 426, 1086
BtgI CCRYGG 2 cut(s) 111, 401
BtsI GCAGTG 2 cut(s) 192, 877
BtsIMutI CAGTG 3 cut(s) 192, 877, 1336
Cac8I GCNNGC 7 cut(s) 100, 190, 199, 358, 1173, 1389, 1410
CaiI CAGNNNCTG 1 cut(s) 1472
CfoI GCGC 2 cut(s) 149, 1171
Cfr13I GGNCC 3 cut(s) 659, 1460, 1469
Cfr9I CCCGGG 1 cut(s) 1232
CseI GACGC 3 cut(s) 94, 711, 1322
CsiI ACCWGGT 1 cut(s) 655
Csp6I GTAC 3 cut(s) 61, 436, 1419
CviAII CATG 3 cut(s) 82, 112, 402
CviQI GTAC 3 cut(s) 61, 436, 1419
DdeI CTNAG 8 cut(s) 369, 432, 856, 942, 966, 1037, 1479, 1598
DinI GGCGCC 1 cut(s) 148
DpnI GATC 5 cut(s) 46, 294, 303, 1533, 1634
DpnII GATC 5 cut(s) 44, 292, 301, 1531, 1632
DraIII CACNNNGTG 1 cut(s) 668
EaeI YGGCCR 1 cut(s) 424
Eam1104I CTCTTC 6 cut(s) 200, 254, 288, 576, 765, 1017
EarI CTCTTC 6 cut(s) 200, 254, 288, 576, 765, 1017
EciI GGCGGA 2 cut(s) 51, 131
Eco130I CCWWGG 2 cut(s) 111, 401
Eco147I AGGCCT 1 cut(s) 374
Eco24I GRGCYC 1 cut(s) 1038
Eco31I GGTCTC 1 cut(s) 803
Eco47I GGWCC 3 cut(s) 659, 1460, 1469
Eco47III AGCGCT 1 cut(s) 1170
Eco57I CTGAAG 1 cut(s) 41
Eco81I CCTNAGG 3 cut(s) 942, 966, 1598
Eco88I CYCGRG 2 cut(s) 378, 1232
EcoNI CCTNNNNNAGG 1 cut(s) 1413
EcoO109I RGGNCCY 1 cut(s) 1469
EcoRI GAATTC 1 cut(s) 956
EcoRII CCWGG 1 cut(s) 655
EcoT14I CCWWGG 2 cut(s) 111, 401
EcoT38I GRGCYC 1 cut(s) 1038
EgeI GGCGCC 1 cut(s) 148
EheI GGCGCC 1 cut(s) 148
ErhI CCWWGG 2 cut(s) 111, 401
FaeI CATG 3 cut(s) 85, 115, 405
FalI AAGNNNNNCTT 2 cut(s) 197, 229
FaqI GGGAC 2 cut(s) 714, 1249
FatI CATG 3 cut(s) 81, 111, 401
Fnu4HI GCNGC 6 cut(s) 34, 103, 145, 1251, 1425, 1590
FriOI GRGCYC 1 cut(s) 1038
Fsp4HI GCNGC 6 cut(s) 34, 103, 145, 1251, 1425, 1590
FspBI CTAG 2 cut(s) 569, 1611
GlaI GCGC 2 cut(s) 148, 1170
GluI GCNGC 6 cut(s) 34, 103, 145, 1251, 1425, 1590
GsaI CCCAGC 1 cut(s) 522
GsuI CTGGAG 1 cut(s) 966
HaeII RGCGCY 2 cut(s) 150, 1172
HaeIII GGCC 3 cut(s) 374, 426, 1086
HapII CCGG 2 cut(s) 1233, 1551
HgaI GACGC 3 cut(s) 94, 711, 1322
HhaI GCGC 2 cut(s) 149, 1171
Hin1I GRCGYC 1 cut(s) 147
Hin1II CATG 3 cut(s) 85, 115, 405
Hin6I GCGC 2 cut(s) 147, 1169
HinP1I GCGC 2 cut(s) 147, 1169
HincII GTYRAC 2 cut(s) 446, 1213
HindII GTYRAC 2 cut(s) 446, 1213
HinfI GANTC 5 cut(s) 166, 280, 607, 1094, 1557
HpaII CCGG 2 cut(s) 1233, 1551
HphI GGTGA 8 cut(s) 731, 827, 893, 901, 1319, 1360, 1506, 1513
Hpy166II GTNNAC 5 cut(s) 446, 458, 662, 1213, 1334
Hpy188I TCNGA 7 cut(s) 10, 55, 301, 745, 1099, 1396, 1480
Hpy8I GTNNAC 5 cut(s) 446, 458, 662, 1213, 1334
Hpy99I CGWCG 4 cut(s) 10, 31, 155, 1127
HpyAV CCTTC 9 cut(s) 303, 446, 667, 827, 871, 1180, 1279, 1339, 1570
HpyCH4III ACNGT 4 cut(s) 74, 443, 1220, 1331
HpyF3I CTNAG 8 cut(s) 369, 432, 856, 942, 966, 1037, 1479, 1598
Hsp92I GRCGYC 1 cut(s) 147
Hsp92II CATG 3 cut(s) 85, 115, 405
HspAI GCGC 2 cut(s) 147, 1169
KasI GGCGCC 1 cut(s) 146
Kzo9I GATC 5 cut(s) 44, 292, 301, 1531, 1632
LguI GCTCTTC 1 cut(s) 200
LmnI GCTCC 4 cut(s) 66, 892, 982, 1384
Lsp1109I GCAGC 3 cut(s) 1237, 1411, 1601
LweI GCATC 8 cut(s) 29, 343, 1090, 1180, 1331, 1436, 1494, 1570
MabI ACCWGGT 1 cut(s) 655
MaeI CTAG 2 cut(s) 569, 1611
MaeIII GTNAC 3 cut(s) 154, 229, 1512
MalI GATC 5 cut(s) 46, 294, 303, 1533, 1634
MboI GATC 5 cut(s) 44, 292, 301, 1531, 1632
MflI RGATCY 2 cut(s) 292, 301
MhlI GDGCHC 4 cut(s) 460, 897, 1038, 1336
MlsI TGGCCA 1 cut(s) 426
MluCI AATT 6 cut(s) 468, 736, 956, 987, 1298, 1488
MluNI TGGCCA 1 cut(s) 426
Mly113I GGCGCC 1 cut(s) 147
MlyI GAGTC 3 cut(s) 274, 601, 1551
MmeI TCCRAC 1 cut(s) 1350
Mox20I TGGCCA 1 cut(s) 426
MscI TGGCCA 1 cut(s) 426
MseI TTAA 6 cut(s) 537, 936, 990, 1227, 1301, 1539
Msp20I TGGCCA 1 cut(s) 426
MspI CCGG 2 cut(s) 1233, 1551
MspR9I CCNGG 4 cut(s) 657, 1233, 1234, 1551
MvaI CCWGG 1 cut(s) 657
MvnI CGCG 1 cut(s) 1530
NarI GGCGCC 1 cut(s) 147
NciI CCSGG 3 cut(s) 1233, 1234, 1551
NcoI CCATGG 2 cut(s) 111, 401
NdeII GATC 5 cut(s) 44, 292, 301, 1531, 1632
NlaIII CATG 3 cut(s) 85, 115, 405
NlaIV GGNNCC 6 cut(s) 148, 303, 972, 1176, 1206, 1386
NmuCI GTSAC 3 cut(s) 154, 229, 1512
NspV TTCGAA 2 cut(s) 251, 960
PceI AGGCCT 1 cut(s) 374
PciSI GCTCTTC 1 cut(s) 200
PcsI WCGNNNNNNNCGW 2 cut(s) 166, 1122
PfeI GAWTC 2 cut(s) 166, 1094
PflFI GACNNNGTC 1 cut(s) 795
PflMI CCANNNNNTGG 1 cut(s) 1457
PkrI GCNGC 6 cut(s) 35, 104, 146, 1252, 1426, 1591
PleI GAGTC 3 cut(s) 274, 601, 1551
PluTI GGCGCC 1 cut(s) 150
PpsI GAGTC 3 cut(s) 274, 601, 1551
PpuMI RGGWCCY 1 cut(s) 1469
PshAI GACNNNNGTC 1 cut(s) 1218
PshBI ATTAAT 1 cut(s) 936
Psp5II RGGWCCY 1 cut(s) 1469
Psp6I CCWGG 1 cut(s) 655
PspFI CCCAGC 1 cut(s) 518
PspGI CCWGG 1 cut(s) 655
PspN4I GGNNCC 6 cut(s) 148, 303, 972, 1176, 1206, 1386
PspPI GGNCC 3 cut(s) 659, 1460, 1469
PspPPI RGGWCCY 1 cut(s) 1469
PstNI CAGNNNCTG 1 cut(s) 1472
PsuI RGATCY 2 cut(s) 292, 301
PsyI GACNNNGTC 1 cut(s) 795
RsaI GTAC 3 cut(s) 62, 437, 1420
RsaNI GTAC 3 cut(s) 61, 436, 1419
SapI GCTCTTC 1 cut(s) 200
SaqAI TTAA 6 cut(s) 537, 936, 990, 1227, 1301, 1539
SatI GCNGC 6 cut(s) 34, 103, 145, 1251, 1425, 1590
Sau3AI GATC 5 cut(s) 44, 292, 301, 1531, 1632
Sau96I GGNCC 3 cut(s) 659, 1460, 1469
SchI GAGTC 3 cut(s) 274, 601, 1551
ScrFI CCNGG 4 cut(s) 657, 1233, 1234, 1551
SduI GDGCHC 4 cut(s) 460, 897, 1038, 1336
SexAI ACCWGGT 1 cut(s) 655
SfaNI GCATC 8 cut(s) 29, 343, 1090, 1180, 1331, 1436, 1494, 1570
SfcI CTRYAG 1 cut(s) 1464
SfoI GGCGCC 1 cut(s) 148
SfuI TTCGAA 2 cut(s) 251, 960
SinI GGWCC 3 cut(s) 659, 1460, 1469
SmaI CCCGGG 1 cut(s) 1234
SmlI CTYRAG 2 cut(s) 609, 1164
SmoI CTYRAG 2 cut(s) 609, 1164
Sse9I AATT 6 cut(s) 468, 736, 956, 987, 1298, 1488
SseBI AGGCCT 1 cut(s) 374
SsiI CCGC 6 cut(s) 33, 36, 42, 102, 116, 144
SspDI GGCGCC 1 cut(s) 146
SspI AATATT 1 cut(s) 906
SspMI CTAG 2 cut(s) 569, 1611
StuI AGGCCT 1 cut(s) 374
StyD4I CCNGG 4 cut(s) 655, 1231, 1232, 1549
StyI CCWWGG 2 cut(s) 111, 401
TaaI ACNGT 4 cut(s) 74, 443, 1220, 1331
TaqI TCGA 5 cut(s) 47, 169, 251, 960, 1433
TaqII GACCGA 1 cut(s) 1370
TasI AATT 6 cut(s) 468, 736, 956, 987, 1298, 1488
TatI WGTACW 1 cut(s) 435
TauI GCSGC 3 cut(s) 36, 105, 147
TfiI GAWTC 2 cut(s) 166, 1094
Tru1I TTAA 6 cut(s) 537, 936, 990, 1227, 1301, 1539
Tru9I TTAA 6 cut(s) 537, 936, 990, 1227, 1301, 1539
TscAI CASTG 3 cut(s) 199, 877, 1336
TseFI GTSAC 3 cut(s) 154, 229, 1512
TseI GCWGC 3 cut(s) 1250, 1424, 1589
Tsp45I GTSAC 3 cut(s) 154, 229, 1512
TspDTI ATGAA 8 cut(s) 205, 605, 636, 650, 846, 858, 936, 1035
TspMI CCCGGG 1 cut(s) 1232
TspRI CASTG 3 cut(s) 199, 877, 1336
Tth111I GACNNNGTC 1 cut(s) 795
Van91I CCANNNNNTGG 1 cut(s) 1457
VneI GTGCAC 2 cut(s) 456, 1332
VpaK11BI GGWCC 3 cut(s) 659, 1460, 1469
VspI ATTAAT 1 cut(s) 936
XagI CCTNNNNNAGG 1 cut(s) 1413
XapI RAATTY 2 cut(s) 736, 956
XbaI TCTAGA 1 cut(s) 568
XmaI CCCGGG 1 cut(s) 1232
XspI CTAG 2 cut(s) 569, 1611
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.