Rw5G001200

Transcription initiation factor IIF subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0001724
Physical Location & Seq
Forward (+)
972867 .. 977530
4664 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G001200.1

Sequence Viewer

Length: 1653 bp
ATGTCGTCGGACTTGTTGCTAAAGCCGTCGTGCGGCGGATGCGGATCGACTTCTGATTTGTACGGAAGCAACTGTAAGCACATGACGCTGTGCTGTGCTTGCGGCAAAACCATGGCGGAGAACAAAGCCAAATGCTACGACTGCGGCGCCGTCGTCACTCGCTTGATTCGAGAATATAATGTGAGGGCAAGCACTGCCAGCGAGAAGAGCTACTTCATTGGTAGGTTTGTGACGGGGTTGCCCAACTTTTCGAAGAAGAAGAGTGCTGAAAATAAATGGACTCTTCGCAAGGATCTTTCGGATCCGAGAAGGAGTAAGGATAAGGATGGACAAGATCAGAAGAAATATAAGGATGTTCGCCCGCCTACTGAGGCCTCTCGGGAGAAGCACAAAAACAAACCATGGGTCTTGGAGGACGAAACTGGCCAGTCTCAGTACAACGGTCAACTCGAAGGTGCACAGTCAGCAATTTACTACCTATTAATGTTGCAAGGAAAGGAGTTTTCTGCTATTCCTGCTGGTTCGTGGTTCAACTTTAACAAGGTTGCACAATATAAGCAACTTACTCTAGAGGAAGCAGAAGAGAAGATGAACAATAGGAAAAAGACTCAAGATGGATATGAAAGATGGATGATGAAATCTGGAACTAATGAACCTGGTCCACTTCGTGAGAAGGAAACTGGTGTGGCTGGTGGTAAGGGACGCAAAAAAATGACTGGTGATGATGATGAGGGAAATTTCTCTGATAAAGGGGAGGAAGATGAGGAAGAAGAGACAGCAAGGAAAAATAGACTTGGTCTCAACAAAAAAGTTGGTGATGACGAGGATGATGAAGGTGGAAATGAAGAAGATGACTTAGATGATGATAGCAGTGAGAAGGGTGATGATTGGGAGCACGAAGAAATATTCACCGATGATGATGAAGCTGTTGGTATTAATCCTGAGGAGAGAGAAGAATTCGAACCTGAGGTTCCAGCTCCCCCAGAAATTAAGCAGGATGATGACGATGATGATAATAATGAAGAGGAGGGTGGGCTCAGCAAATCAGGAAAAGAGTTGAAGAAGCTACTTGGGCGAAGTGGTGGTCTGAATGATTCAGATGCAGAGGACGACGACGACGACGACGACGATATGGATGATGATATTGCCTTGCCTCAAGCGCTGGCACCAAAGCAGAAGGATGCACCCAAACAGGAACCAGTTGACAACAGTCCTATTAAACCCGGGACTTCTGGATCTGCTGCTAAAGCAAACCCATCTTCCTCCAAGTCGGCAAAGGGAAAGAGAAAATTAAATGGTGATGACGCTAAAGCATCCAACAGTGCACCTCCCAAGAAGGTGAAGACTGAAAATGAGCAAAAATCAGTCAAAGAGGAGCCTGCTTCTGAAAGTAGTGTGCCTGCAAAAGGTAGTGCTGTATCCTCGAAAACGGGTTCAACACCATCTGGTGGTCCTACAGGTCCCGTATCTGAGGAAGAAATTAGGGCAGTTTTGATGCAAAAGTCACCCATCACCACACGTGATCTTGTTAATAACTTCCCCGGACGACTCAAATCCAAAGAGGAGAAGGATGCTTTTGCGGCAATCCTGAGGAAAATGTCTAGGATACAGAAGAACAACGGGATCAGCTATGTTGTATTGAGGGAGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005515 GO:0008022
KEGG Pathways
Metabolic & Signaling
ko03022 map03022
Pfam Domains
Protein Families
TFIIF_alpha

Protein Analysis

550

Amino Acids

60.52

Weight (kDa)

4.9

Isoelectric Point (pI)

50.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TFIIF_alpha PF05793 48 - 548 1.9e-121 Transcription initiation factor IIF, alpha subunit (TFIIF-alpha)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 146, 1165
AccB7I CCANNNNNTGG 1 cut(s) 1448
AciI CCGC 8 cut(s) 33, 36, 42, 102, 116, 144, 362, 1580
AclWI GGATC 6 cut(s) 52, 296, 300, 309, 1243, 1631
AcoI YGGCCR 1 cut(s) 424
AcsI RAATTY 2 cut(s) 736, 956
AcvI CACGTG 1 cut(s) 1520
AcyI GRCGYC 1 cut(s) 147
AdeI CACNNNGTG 1 cut(s) 668
AfaI GTAC 2 cut(s) 62, 437
AfeI AGCGCT 1 cut(s) 1161
AfiI CCNNNNNNNGG 3 cut(s) 32, 1406, 1448
AflIII ACRYGT 1 cut(s) 1517
AgsI TTSAA 3 cut(s) 532, 1060, 1437
AjnI CCWGG 1 cut(s) 655
AluBI AGCT 5 cut(s) 210, 926, 977, 1066, 1629
AluI AGCT 5 cut(s) 210, 926, 977, 1066, 1629
Alw21I GWGCWC 3 cut(s) 460, 897, 1327
Alw26I GTCTC 3 cut(s) 435, 767, 803
Alw44I GTGCAC 2 cut(s) 456, 1323
AlwI GGATC 6 cut(s) 52, 296, 300, 309, 1243, 1631
Ama87I CYCGRG 2 cut(s) 378, 1223
Aor51HI AGCGCT 1 cut(s) 1161
AoxI GGCC 2 cut(s) 372, 424
ApaLI GTGCAC 2 cut(s) 456, 1323
ApeKI GCWGC 1 cut(s) 1241
ApoI RAATTY 2 cut(s) 736, 956
ArsI GACNNNNNNTTYG 6 cut(s) 40, 72, 390, 422, 1069, 1101
AseI ATTAAT 2 cut(s) 482, 936
AspLEI GCGC 2 cut(s) 149, 1162
AspS9I GGNCC 3 cut(s) 659, 1451, 1460
AsuC2I CCSGG 3 cut(s) 1224, 1225, 1542
AsuHPI GGTGA 8 cut(s) 731, 827, 893, 901, 1310, 1351, 1497, 1504
AsuII TTCGAA 2 cut(s) 251, 960
AvaI CYCGRG 2 cut(s) 378, 1223
AvaII GGWCC 3 cut(s) 659, 1451, 1460
AxyI CCTNAGG 3 cut(s) 942, 966, 1589
BaeGI GKGCMC 2 cut(s) 460, 1327
BalI TGGCCA 1 cut(s) 426
BamHI GGATCC 1 cut(s) 301
BanI GGYRCC 2 cut(s) 146, 1165
BanII GRGCYC 1 cut(s) 1038
BbrPI CACGTG 1 cut(s) 1520
BbsI GAAGAC 1 cut(s) 1349
Bbv12I GWGCWC 3 cut(s) 460, 897, 1327
BbvI GCAGC 1 cut(s) 1228
BccI CCATC 6 cut(s) 320, 608, 621, 1264, 1450, 1517
BceAI ACGGC 2 cut(s) 10, 134
BciT130I CCWGG 1 cut(s) 657
BciVI GTATCC 2 cut(s) 1429, 1599
BcnI CCSGG 3 cut(s) 1224, 1225, 1542
BcoDI GTCTC 3 cut(s) 435, 767, 803
BfaI CTAG 2 cut(s) 569, 1602
BfmI CTRYAG 1 cut(s) 1455
BfoI RGCGCY 2 cut(s) 150, 1163
BfuI GTATCC 2 cut(s) 1429, 1599
BisI GCNGC 5 cut(s) 34, 103, 145, 1242, 1581
BlpI GCTNAGC 1 cut(s) 1037
BlsI GCNGC 5 cut(s) 35, 104, 146, 1243, 1582
Bme1390I CCNGG 4 cut(s) 657, 1224, 1225, 1542
Bme18I GGWCC 3 cut(s) 659, 1451, 1460
BmeT110I CYCGRG 2 cut(s) 378, 1223
BmgT120I GGNCC 3 cut(s) 659, 1451, 1460
BmiI GGNNCC 7 cut(s) 148, 303, 972, 1167, 1197, 1377, 1462
BmrFI CCNGG 4 cut(s) 657, 1224, 1225, 1542
BmsI GCATC 6 cut(s) 29, 1090, 1171, 1322, 1485, 1561
BoxI GACNNNNGTC 1 cut(s) 1209
BpiI GAAGAC 1 cut(s) 1349
Bpu1102I GCTNAGC 1 cut(s) 1037
Bpu14I TTCGAA 2 cut(s) 251, 960
BpuEI CTTGAG 2 cut(s) 594, 1140
BpuMI CCSGG 3 cut(s) 1224, 1225, 1542
BsaAI YACGTR 1 cut(s) 1520
BsaBI GATNNNNATC 1 cut(s) 43
BsaHI GRCGYC 1 cut(s) 147
BsaI GGTCTC 1 cut(s) 803
BsaJI CCNNGG 4 cut(s) 111, 401, 1223, 1540
Bsc4I CCNNNNNNNGG 3 cut(s) 32, 1406, 1448
Bse1I ACTGG 5 cut(s) 427, 427, 685, 721, 1199
Bse21I CCTNAGG 3 cut(s) 942, 966, 1589
Bse8I GATNNNNATC 1 cut(s) 43
BseBI CCWGG 1 cut(s) 657
BseDI CCNNGG 4 cut(s) 111, 401, 1223, 1540
BseJI GATNNNNATC 1 cut(s) 43
BseLI CCNNNNNNNGG 3 cut(s) 32, 1406, 1448
BseMII CTCAG 7 cut(s) 360, 446, 933, 957, 1051, 1461, 1580
BseNI ACTGG 5 cut(s) 427, 427, 685, 721, 1199
BseRI GAGGAG 4 cut(s) 959, 1040, 1388, 1577
BseSI GKGCMC 2 cut(s) 460, 1327
BseXI GCAGC 1 cut(s) 1228
BshFI GGCC 2 cut(s) 374, 426
BshNI GGYRCC 2 cut(s) 146, 1165
BsiHKAI GWGCWC 3 cut(s) 460, 897, 1327
BsiHKCI CYCGRG 2 cut(s) 378, 1223
BsiSI CCGG 2 cut(s) 1224, 1542
BslFI GGGAC 3 cut(s) 714, 1240, 1446
BslI CCNNNNNNNGG 3 cut(s) 32, 1406, 1448
BsmAI GTCTC 3 cut(s) 435, 767, 803
BsmFI GGGAC 3 cut(s) 714, 1240, 1446
BsnI GGCC 2 cut(s) 374, 426
Bso31I GGTCTC 1 cut(s) 803
BsoBI CYCGRG 2 cut(s) 378, 1223
Bsp119I TTCGAA 2 cut(s) 251, 960
Bsp1286I GDGCHC 4 cut(s) 460, 897, 1038, 1327
Bsp143I GATC 7 cut(s) 44, 292, 301, 334, 1235, 1522, 1623
Bsp1720I GCTNAGC 1 cut(s) 1037
Bsp19I CCATGG 2 cut(s) 111, 401
BspACI CCGC 8 cut(s) 33, 36, 42, 102, 116, 144, 362, 1580
BspANI GGCC 2 cut(s) 374, 426
BspCNI CTCAG 7 cut(s) 361, 445, 934, 958, 1050, 1462, 1581
BspLI GGNNCC 7 cut(s) 148, 303, 972, 1167, 1197, 1377, 1462
BspPI GGATC 6 cut(s) 52, 296, 300, 309, 1243, 1631
BspQI GCTCTTC 1 cut(s) 200
BspT104I TTCGAA 2 cut(s) 251, 960
BspT107I GGYRCC 2 cut(s) 146, 1165
BspTNI GGTCTC 1 cut(s) 803
BsrI ACTGG 5 cut(s) 427, 427, 685, 721, 1199
BssECI CCNNGG 4 cut(s) 111, 401, 1223, 1540
BssMI GATC 7 cut(s) 44, 292, 301, 334, 1235, 1522, 1623
BssNI GRCGYC 1 cut(s) 147
BssT1I CCWWGG 2 cut(s) 111, 401
Bst2UI CCWGG 1 cut(s) 657
Bst4CI ACNGT 5 cut(s) 74, 443, 462, 1211, 1322
Bst6I CTCTTC 6 cut(s) 200, 254, 288, 576, 765, 1017
BstACI GRCGYC 1 cut(s) 147
BstAPI GCANNNNNTGC 1 cut(s) 194
BstBAI YACGTR 1 cut(s) 1520
BstBI TTCGAA 2 cut(s) 251, 960
BstC8I GCNNGC 7 cut(s) 100, 190, 199, 362, 1164, 1380, 1401
BstDEI CTNAG 8 cut(s) 369, 432, 856, 942, 966, 1037, 1470, 1589
BstDSI CCRYGG 2 cut(s) 111, 401
BstENI CCTNNNNNAGG 1 cut(s) 1404
BstH2I RGCGCY 2 cut(s) 150, 1163
BstHHI GCGC 2 cut(s) 149, 1162
BstKTI GATC 7 cut(s) 47, 295, 304, 337, 1238, 1525, 1626
BstMAI GTCTC 3 cut(s) 435, 767, 803
BstMBI GATC 7 cut(s) 44, 292, 301, 334, 1235, 1522, 1623
BstNI CCWGG 1 cut(s) 657
BstPAI GACNNNNGTC 1 cut(s) 1209
BstSCI CCNGG 4 cut(s) 655, 1222, 1223, 1540
BstSFI CTRYAG 1 cut(s) 1455
BstSLI GKGCMC 2 cut(s) 460, 1327
BstV1I GCAGC 1 cut(s) 1228
BstV2I GAAGAC 1 cut(s) 1349
BstX2I RGATCY 3 cut(s) 292, 301, 1235
BstYI RGATCY 3 cut(s) 292, 301, 1235
Bsu36I CCTNAGG 3 cut(s) 942, 966, 1589
BsuI GTATCC 2 cut(s) 1429, 1599
BsuRI GGCC 2 cut(s) 374, 426
BtgI CCRYGG 2 cut(s) 111, 401
BtsI GCAGTG 2 cut(s) 192, 877
BtsIMutI CAGTG 3 cut(s) 192, 877, 1327
Cac8I GCNNGC 7 cut(s) 100, 190, 199, 362, 1164, 1380, 1401
CfoI GCGC 2 cut(s) 149, 1162
Cfr13I GGNCC 3 cut(s) 659, 1451, 1460
Cfr9I CCCGGG 1 cut(s) 1223
CseI GACGC 3 cut(s) 94, 711, 1313
CsiI ACCWGGT 1 cut(s) 655
Csp6I GTAC 2 cut(s) 61, 436
CviAII CATG 3 cut(s) 82, 112, 402
CviQI GTAC 2 cut(s) 61, 436
DdeI CTNAG 8 cut(s) 369, 432, 856, 942, 966, 1037, 1470, 1589
DinI GGCGCC 1 cut(s) 148
DpnI GATC 7 cut(s) 46, 294, 303, 336, 1237, 1524, 1625
DpnII GATC 7 cut(s) 44, 292, 301, 334, 1235, 1522, 1623
DraIII CACNNNGTG 1 cut(s) 668
EaeI YGGCCR 1 cut(s) 424
Eam1104I CTCTTC 6 cut(s) 200, 254, 288, 576, 765, 1017
EarI CTCTTC 6 cut(s) 200, 254, 288, 576, 765, 1017
EciI GGCGGA 2 cut(s) 51, 131
Eco130I CCWWGG 2 cut(s) 111, 401
Eco147I AGGCCT 1 cut(s) 374
Eco24I GRGCYC 1 cut(s) 1038
Eco31I GGTCTC 1 cut(s) 803
Eco47I GGWCC 3 cut(s) 659, 1451, 1460
Eco47III AGCGCT 1 cut(s) 1161
Eco72I CACGTG 1 cut(s) 1520
Eco81I CCTNAGG 3 cut(s) 942, 966, 1589
Eco88I CYCGRG 2 cut(s) 378, 1223
EcoNI CCTNNNNNAGG 1 cut(s) 1404
EcoO109I RGGNCCY 1 cut(s) 1460
EcoRI GAATTC 1 cut(s) 956
EcoRII CCWGG 1 cut(s) 655
EcoT14I CCWWGG 2 cut(s) 111, 401
EcoT38I GRGCYC 1 cut(s) 1038
EgeI GGCGCC 1 cut(s) 148
EheI GGCGCC 1 cut(s) 148
ErhI CCWWGG 2 cut(s) 111, 401
FaeI CATG 3 cut(s) 85, 115, 405
FaiI YATR 9 cut(s) 83, 113, 177, 348, 403, 555, 621, 1133, 1632
FalI AAGNNNNNCTT 2 cut(s) 197, 229
FaqI GGGAC 3 cut(s) 714, 1240, 1446
FatI CATG 3 cut(s) 81, 111, 401
FauI CCCGC 1 cut(s) 369
Fnu4HI GCNGC 5 cut(s) 34, 103, 145, 1242, 1581
FriOI GRGCYC 1 cut(s) 1038
Fsp4HI GCNGC 5 cut(s) 34, 103, 145, 1242, 1581
FspBI CTAG 2 cut(s) 569, 1602
GlaI GCGC 2 cut(s) 148, 1161
GluI GCNGC 5 cut(s) 34, 103, 145, 1242, 1581
HaeII RGCGCY 2 cut(s) 150, 1163
HaeIII GGCC 2 cut(s) 374, 426
HapII CCGG 2 cut(s) 1224, 1542
HgaI GACGC 3 cut(s) 94, 711, 1313
HhaI GCGC 2 cut(s) 149, 1162
Hin1I GRCGYC 1 cut(s) 147
Hin1II CATG 3 cut(s) 85, 115, 405
Hin6I GCGC 2 cut(s) 147, 1160
HinP1I GCGC 2 cut(s) 147, 1160
HincII GTYRAC 2 cut(s) 446, 1204
HindII GTYRAC 2 cut(s) 446, 1204
HinfI GANTC 5 cut(s) 166, 280, 607, 1094, 1548
HpaII CCGG 2 cut(s) 1224, 1542
HphI GGTGA 8 cut(s) 731, 827, 893, 901, 1310, 1351, 1497, 1504
Hpy166II GTNNAC 5 cut(s) 446, 458, 662, 1204, 1325
Hpy8I GTNNAC 5 cut(s) 446, 458, 662, 1204, 1325
Hpy99I CGWCG 9 cut(s) 10, 31, 155, 1115, 1118, 1121, 1124, 1127, 1130
HpyAV CCTTC 8 cut(s) 303, 446, 667, 827, 871, 1171, 1330, 1561
HpyCH4III ACNGT 5 cut(s) 74, 443, 462, 1211, 1322
HpyCH4IV ACGT 1 cut(s) 1519
HpyCH4V TGCA 8 cut(s) 458, 490, 548, 1103, 1184, 1325, 1403, 1498
HpyF3I CTNAG 8 cut(s) 369, 432, 856, 942, 966, 1037, 1470, 1589
HpySE526I ACGT 1 cut(s) 1519
Hsp92I GRCGYC 1 cut(s) 147
Hsp92II CATG 3 cut(s) 85, 115, 405
HspAI GCGC 2 cut(s) 147, 1160
KasI GGCGCC 1 cut(s) 146
Kzo9I GATC 7 cut(s) 44, 292, 301, 334, 1235, 1522, 1623
LguI GCTCTTC 1 cut(s) 200
LmnI GCTCC 3 cut(s) 892, 982, 1375
Lsp1109I GCAGC 1 cut(s) 1228
LweI GCATC 6 cut(s) 29, 1090, 1171, 1322, 1485, 1561
MabI ACCWGGT 1 cut(s) 655
MaeI CTAG 2 cut(s) 569, 1602
MaeII ACGT 1 cut(s) 1519
MaeIII GTNAC 3 cut(s) 154, 229, 1503
MalI GATC 7 cut(s) 46, 294, 303, 336, 1237, 1524, 1625
MboI GATC 7 cut(s) 44, 292, 301, 334, 1235, 1522, 1623
MflI RGATCY 3 cut(s) 292, 301, 1235
MhlI GDGCHC 4 cut(s) 460, 897, 1038, 1327
MlsI TGGCCA 1 cut(s) 426
MluCI AATT 6 cut(s) 468, 736, 956, 987, 1289, 1479
MluNI TGGCCA 1 cut(s) 426
Mly113I GGCGCC 1 cut(s) 147
MlyI GAGTC 3 cut(s) 274, 601, 1542
MmeI TCCRAC 1 cut(s) 1341
Mox20I TGGCCA 1 cut(s) 426
MscI TGGCCA 1 cut(s) 426
MseI TTAA 7 cut(s) 482, 537, 936, 990, 1218, 1292, 1530
Msp20I TGGCCA 1 cut(s) 426
MspI CCGG 2 cut(s) 1224, 1542
MspR9I CCNGG 4 cut(s) 657, 1224, 1225, 1542
MvaI CCWGG 1 cut(s) 657
NarI GGCGCC 1 cut(s) 147
NciI CCSGG 3 cut(s) 1224, 1225, 1542
NcoI CCATGG 2 cut(s) 111, 401
NdeII GATC 7 cut(s) 44, 292, 301, 334, 1235, 1522, 1623
NlaIII CATG 3 cut(s) 85, 115, 405
NlaIV GGNNCC 7 cut(s) 148, 303, 972, 1167, 1197, 1377, 1462
NmuCI GTSAC 3 cut(s) 154, 229, 1503
NspV TTCGAA 2 cut(s) 251, 960
PceI AGGCCT 1 cut(s) 374
PciSI GCTCTTC 1 cut(s) 200
PcsI WCGNNNNNNNCGW 6 cut(s) 166, 447, 1116, 1119, 1122, 1125
PfeI GAWTC 2 cut(s) 166, 1094
PflFI GACNNNGTC 1 cut(s) 795
PflMI CCANNNNNTGG 1 cut(s) 1448
PkrI GCNGC 5 cut(s) 35, 104, 146, 1243, 1582
PleI GAGTC 3 cut(s) 274, 601, 1542
PluTI GGCGCC 1 cut(s) 150
PmaCI CACGTG 1 cut(s) 1520
PmlI CACGTG 1 cut(s) 1520
PpsI GAGTC 3 cut(s) 274, 601, 1542
Ppu21I YACGTR 1 cut(s) 1520
PpuMI RGGWCCY 1 cut(s) 1460
PshAI GACNNNNGTC 1 cut(s) 1209
PshBI ATTAAT 2 cut(s) 482, 936
Psp5II RGGWCCY 1 cut(s) 1460
Psp6I CCWGG 1 cut(s) 655
PspCI CACGTG 1 cut(s) 1520
PspGI CCWGG 1 cut(s) 655
PspN4I GGNNCC 7 cut(s) 148, 303, 972, 1167, 1197, 1377, 1462
PspPI GGNCC 3 cut(s) 659, 1451, 1460
PspPPI RGGWCCY 1 cut(s) 1460
PsuI RGATCY 3 cut(s) 292, 301, 1235
PsyI GACNNNGTC 1 cut(s) 795
RsaI GTAC 2 cut(s) 62, 437
RsaNI GTAC 2 cut(s) 61, 436
SapI GCTCTTC 1 cut(s) 200
SaqAI TTAA 7 cut(s) 482, 537, 936, 990, 1218, 1292, 1530
SatI GCNGC 5 cut(s) 34, 103, 145, 1242, 1581
Sau3AI GATC 7 cut(s) 44, 292, 301, 334, 1235, 1522, 1623
Sau96I GGNCC 3 cut(s) 659, 1451, 1460
SchI GAGTC 3 cut(s) 274, 601, 1542
ScrFI CCNGG 4 cut(s) 657, 1224, 1225, 1542
SduI GDGCHC 4 cut(s) 460, 897, 1038, 1327
SexAI ACCWGGT 1 cut(s) 655
SfaNI GCATC 6 cut(s) 29, 1090, 1171, 1322, 1485, 1561
SfcI CTRYAG 1 cut(s) 1455
SfoI GGCGCC 1 cut(s) 148
SfuI TTCGAA 2 cut(s) 251, 960
SinI GGWCC 3 cut(s) 659, 1451, 1460
SmaI CCCGGG 1 cut(s) 1225
SmlI CTYRAG 2 cut(s) 609, 1155
SmoI CTYRAG 2 cut(s) 609, 1155
Sse9I AATT 6 cut(s) 468, 736, 956, 987, 1289, 1479
SseBI AGGCCT 1 cut(s) 374
SsiI CCGC 8 cut(s) 33, 36, 42, 102, 116, 144, 362, 1580
SspDI GGCGCC 1 cut(s) 146
SspI AATATT 1 cut(s) 906
SspMI CTAG 2 cut(s) 569, 1602
StuI AGGCCT 1 cut(s) 374
StyD4I CCNGG 4 cut(s) 655, 1222, 1223, 1540
StyI CCWWGG 2 cut(s) 111, 401
TaaI ACNGT 5 cut(s) 74, 443, 462, 1211, 1322
TaiI ACGT 1 cut(s) 1522
TaqI TCGA 6 cut(s) 47, 169, 251, 450, 960, 1424
TasI AATT 6 cut(s) 468, 736, 956, 987, 1289, 1479
TatI WGTACW 1 cut(s) 435
TauI GCSGC 4 cut(s) 36, 105, 147, 1583
TfiI GAWTC 2 cut(s) 166, 1094
Tru1I TTAA 7 cut(s) 482, 537, 936, 990, 1218, 1292, 1530
Tru9I TTAA 7 cut(s) 482, 537, 936, 990, 1218, 1292, 1530
TscAI CASTG 3 cut(s) 199, 877, 1327
TseFI GTSAC 3 cut(s) 154, 229, 1503
TseI GCWGC 1 cut(s) 1241
Tsp45I GTSAC 3 cut(s) 154, 229, 1503
TspDTI ATGAA 9 cut(s) 205, 605, 636, 650, 666, 846, 858, 936, 1035
TspGWI ACGGA 1 cut(s) 78
TspMI CCCGGG 1 cut(s) 1223
TspRI CASTG 3 cut(s) 199, 877, 1327
Tth111I GACNNNGTC 1 cut(s) 795
Van91I CCANNNNNTGG 1 cut(s) 1448
VneI GTGCAC 2 cut(s) 456, 1323
VpaK11BI GGWCC 3 cut(s) 659, 1451, 1460
VspI ATTAAT 2 cut(s) 482, 936
XagI CCTNNNNNAGG 1 cut(s) 1404
XapI RAATTY 2 cut(s) 736, 956
XbaI TCTAGA 1 cut(s) 568
XmaI CCCGGG 1 cut(s) 1223
XspI CTAG 2 cut(s) 569, 1602
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.