Rh2DG465300

Transcription initiation factor IIF subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0001724
Physical Location & Seq
Forward (+)
67372322 .. 67374479
2158 bp
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UTR
Exon/CDS
Intron
Rh2DG465300.1

Sequence Viewer

Length: 537 bp
ATGGATGATGAAATTGGAACTAACGAACCTGGTCCACTTCGTGAGAAGGAAACTGGTGTGGCTGGTGGTAAGGGACGCAAAAAAATGACTGGTGATGATGATGAGGGAAATTTCTCTGATAAAGGGGAGGAAGATGAGGAAGAAGAGACAGCAAGGAAAAATAGACTTGGTCTCAATAAAAAAGGTGGTGATGATGACGATGATGAAGGTGGAAATGAAGAAGATGACTTAGATGATGATAGCAGTGAGAAGGGTGATGATTGGGAGCATGAAGAAATATTCACCGATGATGATGAAGCTGTTGGTATTAATCCTGAGGAGAGAGAAGAATTCGAACCTGAGGTTCCAGCTCCTCCAGAAATTAAGCAGGATGATGACGATGATGATAATAATGAAGAGGAGGGTGGGCTCAGCAAATCAGGAAAAGAGTTGAAGAAGCAACTTGGGCGAAGTGGTGGCCTGAATGATTCAGATGCAGAGGACGACGATGATATGGATGATGATATTGCCTTGCCTCAAGCGCTTGCACCAAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005515 GO:0008022
KEGG Pathways
Metabolic & Signaling
ko03022 map03022
Pfam Domains
Protein Families
TFIIF_alpha

Protein Analysis

178

Amino Acids

19.52

Weight (kDa)

4.05

Isoelectric Point (pI)

55.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TFIIF_alpha PF05793 18 - 167 7.8e-27 Transcription initiation factor IIF, alpha subunit (TFIIF-alpha)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 2 cut(s) 109, 329
AdeI CACNNNGTG 1 cut(s) 41
AfeI AGCGCT 1 cut(s) 522
AgsI TTSAA 1 cut(s) 433
AjnI CCWGG 1 cut(s) 28
AluBI AGCT 2 cut(s) 299, 350
AluI AGCT 2 cut(s) 299, 350
Alw26I GTCTC 2 cut(s) 140, 176
Aor51HI AGCGCT 1 cut(s) 522
AoxI GGCC 1 cut(s) 457
ApoI RAATTY 2 cut(s) 109, 329
AseI ATTAAT 1 cut(s) 309
AspLEI GCGC 1 cut(s) 523
AspS9I GGNCC 1 cut(s) 32
AsuHPI GGTGA 4 cut(s) 104, 200, 266, 274
AsuII TTCGAA 1 cut(s) 333
AvaII GGWCC 1 cut(s) 32
AxyI CCTNAGG 2 cut(s) 315, 339
BanII GRGCYC 1 cut(s) 411
BciT130I CCWGG 1 cut(s) 30
BcoDI GTCTC 2 cut(s) 140, 176
BfoI RGCGCY 1 cut(s) 524
BlpI GCTNAGC 1 cut(s) 410
Bme1390I CCNGG 1 cut(s) 30
Bme18I GGWCC 1 cut(s) 32
BmgT120I GGNCC 1 cut(s) 32
BmiI GGNNCC 1 cut(s) 345
BmrFI CCNGG 1 cut(s) 30
BmsI GCATC 1 cut(s) 463
BpmI CTGGAG 1 cut(s) 339
Bpu1102I GCTNAGC 1 cut(s) 410
Bpu14I TTCGAA 1 cut(s) 333
BpuEI CTTGAG 1 cut(s) 501
BsaI GGTCTC 1 cut(s) 176
Bse1I ACTGG 2 cut(s) 58, 94
Bse21I CCTNAGG 2 cut(s) 315, 339
BseBI CCWGG 1 cut(s) 30
BseGI GGATG 3 cut(s) 10, 376, 502
BseMII CTCAG 3 cut(s) 306, 330, 424
BseNI ACTGG 2 cut(s) 58, 94
BseRI GAGGAG 3 cut(s) 332, 342, 413
BshFI GGCC 1 cut(s) 459
BslFI GGGAC 1 cut(s) 87
BsmAI GTCTC 2 cut(s) 140, 176
BsmFI GGGAC 1 cut(s) 87
BsnI GGCC 1 cut(s) 459
Bso31I GGTCTC 1 cut(s) 176
Bsp119I TTCGAA 1 cut(s) 333
Bsp1286I GDGCHC 1 cut(s) 411
Bsp1720I GCTNAGC 1 cut(s) 410
BspANI GGCC 1 cut(s) 459
BspCNI CTCAG 3 cut(s) 307, 331, 423
BspLI GGNNCC 1 cut(s) 345
BspT104I TTCGAA 1 cut(s) 333
BspTNI GGTCTC 1 cut(s) 176
BsrI ACTGG 2 cut(s) 58, 94
Bst2UI CCWGG 1 cut(s) 30
Bst6I CTCTTC 2 cut(s) 138, 390
BstBI TTCGAA 1 cut(s) 333
BstC8I GCNNGC 1 cut(s) 525
BstDEI CTNAG 4 cut(s) 229, 315, 339, 410
BstF5I GGATG 3 cut(s) 10, 376, 502
BstH2I RGCGCY 1 cut(s) 524
BstHHI GCGC 1 cut(s) 523
BstMAI GTCTC 2 cut(s) 140, 176
BstMWI GCNNNNNNNGC 2 cut(s) 445, 520
BstNI CCWGG 1 cut(s) 30
BstSCI CCNGG 1 cut(s) 28
Bsu36I CCTNAGG 2 cut(s) 315, 339
BsuRI GGCC 1 cut(s) 459
BtsCI GGATG 3 cut(s) 10, 376, 502
BtsI GCAGTG 1 cut(s) 250
BtsIMutI CAGTG 1 cut(s) 250
Cac8I GCNNGC 1 cut(s) 525
CfoI GCGC 1 cut(s) 523
Cfr13I GGNCC 1 cut(s) 32
CseI GACGC 1 cut(s) 84
CsiI ACCWGGT 1 cut(s) 28
CviAII CATG 1 cut(s) 269
CviJI RGCY 5 cut(s) 62, 299, 350, 409, 459
CviKI_1 RGCY 5 cut(s) 62, 299, 350, 409, 459
DdeI CTNAG 4 cut(s) 229, 315, 339, 410
DraIII CACNNNGTG 1 cut(s) 41
Eam1104I CTCTTC 2 cut(s) 138, 390
EarI CTCTTC 2 cut(s) 138, 390
Eco24I GRGCYC 1 cut(s) 411
Eco31I GGTCTC 1 cut(s) 176
Eco47I GGWCC 1 cut(s) 32
Eco47III AGCGCT 1 cut(s) 522
Eco81I CCTNAGG 2 cut(s) 315, 339
EcoRI GAATTC 1 cut(s) 329
EcoRII CCWGG 1 cut(s) 28
EcoT38I GRGCYC 1 cut(s) 411
FaeI CATG 1 cut(s) 272
FaiI YATR 2 cut(s) 270, 494
FaqI GGGAC 1 cut(s) 87
FatI CATG 1 cut(s) 268
FokI GGATG 3 cut(s) 17, 383, 509
FriOI GRGCYC 1 cut(s) 411
GlaI GCGC 1 cut(s) 522
GsuI CTGGAG 1 cut(s) 339
HaeII RGCGCY 1 cut(s) 524
HaeIII GGCC 1 cut(s) 459
HgaI GACGC 1 cut(s) 84
HhaI GCGC 1 cut(s) 523
Hin1II CATG 1 cut(s) 272
Hin6I GCGC 1 cut(s) 521
HinP1I GCGC 1 cut(s) 521
HinfI GANTC 1 cut(s) 467
HphI GGTGA 4 cut(s) 104, 200, 266, 274
Hpy166II GTNNAC 1 cut(s) 35
Hpy188I TCNGA 2 cut(s) 118, 472
Hpy188III TCNNGA 4 cut(s) 41, 314, 356, 420
Hpy8I GTNNAC 1 cut(s) 35
Hpy99I CGWCG 1 cut(s) 488
HpyAV CCTTC 3 cut(s) 40, 200, 244
HpyCH4V TGCA 2 cut(s) 476, 527
HpyF10VI GCNNNNNNNGC 2 cut(s) 445, 520
HpyF3I CTNAG 4 cut(s) 229, 315, 339, 410
Hsp92II CATG 1 cut(s) 272
HspAI GCGC 1 cut(s) 521
LmnI GCTCC 2 cut(s) 265, 355
LweI GCATC 1 cut(s) 463
MabI ACCWGGT 1 cut(s) 28
MboII GAAGA 9 cut(s) 143, 152, 155, 230, 233, 284, 338, 407, 445
MhlI GDGCHC 1 cut(s) 411
MluCI AATT 4 cut(s) 12, 109, 329, 360
MseI TTAA 2 cut(s) 309, 363
MspR9I CCNGG 1 cut(s) 30
MvaI CCWGG 1 cut(s) 30
MwoI GCNNNNNNNGC 2 cut(s) 445, 520
NlaIII CATG 1 cut(s) 272
NlaIV GGNNCC 1 cut(s) 345
NspV TTCGAA 1 cut(s) 333
PfeI GAWTC 1 cut(s) 467
PflFI GACNNNGTC 1 cut(s) 168
PshBI ATTAAT 1 cut(s) 309
Psp6I CCWGG 1 cut(s) 28
PspGI CCWGG 1 cut(s) 28
PspN4I GGNNCC 1 cut(s) 345
PspPI GGNCC 1 cut(s) 32
PsyI GACNNNGTC 1 cut(s) 168
SaqAI TTAA 2 cut(s) 309, 363
Sau96I GGNCC 1 cut(s) 32
ScrFI CCNGG 1 cut(s) 30
SduI GDGCHC 1 cut(s) 411
SetI ASST 7 cut(s) 31, 187, 211, 301, 340, 345, 352
SexAI ACCWGGT 1 cut(s) 28
SfaNI GCATC 1 cut(s) 463
SfuI TTCGAA 1 cut(s) 333
SinI GGWCC 1 cut(s) 32
SmlI CTYRAG 1 cut(s) 516
SmoI CTYRAG 1 cut(s) 516
Sse9I AATT 4 cut(s) 12, 109, 329, 360
SspI AATATT 1 cut(s) 279
StyD4I CCNGG 1 cut(s) 28
TaqI TCGA 1 cut(s) 333
TasI AATT 4 cut(s) 12, 109, 329, 360
TfiI GAWTC 1 cut(s) 467
Tru1I TTAA 2 cut(s) 309, 363
Tru9I TTAA 2 cut(s) 309, 363
TscAI CASTG 1 cut(s) 250
TspDTI ATGAA 6 cut(s) 24, 219, 231, 285, 309, 408
TspRI CASTG 1 cut(s) 250
Tth111I GACNNNGTC 1 cut(s) 168
VpaK11BI GGWCC 1 cut(s) 32
VspI ATTAAT 1 cut(s) 309
XapI RAATTY 2 cut(s) 109, 329
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.