Rh2BG455300

Transcription initiation factor IIF subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0001724
Physical Location & Seq
Forward (+)
64453847 .. 64456409
2563 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG455300.1

Sequence Viewer

Length: 819 bp
ATGGATGATGAAATTGGAACTAACGAACCTGGTCCACTTCGTGAGAAGGAAACTGGTGTGGCTGGTGGTAAGGGACGCAAAAAAATGACTGGTGATGATGATGAGGGAAATTTCTCTGATAAAGGGGAGGAAGATGAGGAAGAAGAGACAGCAAGGAAAAATAGACTTGGTCTCAATAAAAAAGGTGGTGATGATGACGATGATGAAGGTGGAAATGAAGAAGATGACTTAGATGATGATAGCAGTGAGAAGGGTGATGATTGGGAGCATGAAGAAATATTCACCGATGATGATGAAGCTGTTGGTATTAATCCTGAGGAGAGAGAAGAATTCGAACCTGAGGTTCCAGCTCCTCCAGAAATTAAGCAGGATGATGACGATGATGATAATAATGAAGAGGAGGGTGGGCTCAGCAAATCAGGAAAAGAGTTGAAGAAGCAACTTGGGCGAAGTGGTGGCCTGAATGATTCAGATGCAGAGGACGACGATGATATGGATGATGATATTGCCTTGCCTCAAGCGCTTGCACCAAATCCTATTAAACCCGGGACTTCTGGATCTGCTGCTAAAGCAAACCCATCTGCCTCCAAGTCGGCGAAGGGAAAGAGAAAATTAAATGGTGATGACGCTAAAGCATCCAACAGTGCACCTCCCAAGAAGGTGAAGACCGAAAATGAGCAAAAATCAGTCAAAGAGGAGCCTGCTTCTGAAAGTAGTGTGCCTGCAAAAGGTACTGCTGCATCCTCGAAAACGGGTACAACACCATCTGGTGGTCCTACAGGTCCTGTATCTGAGGAAGAAATTAGGGCGGTGTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005515 GO:0008022
KEGG Pathways
Metabolic & Signaling
ko03022 map03022
Pfam Domains
Protein Families
TFIIF_alpha

Protein Analysis

272

Amino Acids

28.9

Weight (kDa)

4.21

Isoelectric Point (pI)

52.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TFIIF_alpha PF05793 19 - 271 1e-36 Transcription initiation factor IIF, alpha subunit (TFIIF-alpha)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 770
AciI CCGC 1 cut(s) 809
AclWI GGATC 1 cut(s) 565
AcsI RAATTY 2 cut(s) 109, 329
AdeI CACNNNGTG 1 cut(s) 41
AfaI GTAC 2 cut(s) 733, 757
AfeI AGCGCT 1 cut(s) 522
AfiI CCNNNNNNNGG 2 cut(s) 728, 770
AgsI TTSAA 1 cut(s) 433
AjnI CCWGG 1 cut(s) 28
AluBI AGCT 2 cut(s) 299, 350
AluI AGCT 2 cut(s) 299, 350
Alw21I GWGCWC 1 cut(s) 649
Alw26I GTCTC 2 cut(s) 140, 176
Alw44I GTGCAC 1 cut(s) 645
AlwI GGATC 1 cut(s) 565
AlwNI CAGNNNCTG 1 cut(s) 785
Ama87I CYCGRG 1 cut(s) 545
Aor51HI AGCGCT 1 cut(s) 522
AoxI GGCC 1 cut(s) 457
ApaLI GTGCAC 1 cut(s) 645
ApeKI GCWGC 2 cut(s) 563, 737
ApoI RAATTY 2 cut(s) 109, 329
AseI ATTAAT 1 cut(s) 309
AspLEI GCGC 1 cut(s) 523
AspS9I GGNCC 3 cut(s) 32, 773, 782
AsuC2I CCSGG 2 cut(s) 546, 547
AsuHPI GGTGA 6 cut(s) 104, 200, 266, 274, 632, 673
AsuII TTCGAA 1 cut(s) 333
AvaI CYCGRG 1 cut(s) 545
AvaII GGWCC 3 cut(s) 32, 773, 782
AxyI CCTNAGG 2 cut(s) 315, 339
BaeGI GKGCMC 1 cut(s) 649
BanII GRGCYC 1 cut(s) 411
BbsI GAAGAC 1 cut(s) 671
Bbv12I GWGCWC 1 cut(s) 649
BbvI GCAGC 2 cut(s) 550, 724
BccI CCATC 2 cut(s) 586, 772
BciT130I CCWGG 1 cut(s) 30
BcnI CCSGG 2 cut(s) 546, 547
BcoDI GTCTC 2 cut(s) 140, 176
BfmI CTRYAG 1 cut(s) 777
BfoI RGCGCY 1 cut(s) 524
BisI GCNGC 2 cut(s) 564, 738
BlpI GCTNAGC 1 cut(s) 410
BlsI GCNGC 2 cut(s) 565, 739
Bme1390I CCNGG 3 cut(s) 30, 546, 547
Bme18I GGWCC 3 cut(s) 32, 773, 782
BmeT110I CYCGRG 1 cut(s) 545
BmgT120I GGNCC 3 cut(s) 32, 773, 782
BmiI GGNNCC 2 cut(s) 345, 699
BmrFI CCNGG 3 cut(s) 30, 546, 547
BmsI GCATC 3 cut(s) 463, 644, 749
BpiI GAAGAC 1 cut(s) 671
BpmI CTGGAG 1 cut(s) 339
Bpu1102I GCTNAGC 1 cut(s) 410
Bpu14I TTCGAA 1 cut(s) 333
BpuEI CTTGAG 1 cut(s) 501
BpuMI CCSGG 2 cut(s) 546, 547
BsaI GGTCTC 1 cut(s) 176
BsaJI CCNNGG 1 cut(s) 545
Bsc4I CCNNNNNNNGG 2 cut(s) 728, 770
Bse1I ACTGG 2 cut(s) 58, 94
Bse21I CCTNAGG 2 cut(s) 315, 339
BseBI CCWGG 1 cut(s) 30
BseDI CCNNGG 1 cut(s) 545
BseGI GGATG 5 cut(s) 10, 376, 502, 635, 740
BseLI CCNNNNNNNGG 2 cut(s) 728, 770
BseMII CTCAG 4 cut(s) 306, 330, 424, 783
BseNI ACTGG 2 cut(s) 58, 94
BseRI GAGGAG 4 cut(s) 332, 342, 413, 710
BseSI GKGCMC 1 cut(s) 649
BseXI GCAGC 2 cut(s) 550, 724
BshFI GGCC 1 cut(s) 459
BsiHKAI GWGCWC 1 cut(s) 649
BsiHKCI CYCGRG 1 cut(s) 545
BsiSI CCGG 1 cut(s) 546
BslFI GGGAC 2 cut(s) 87, 562
BslI CCNNNNNNNGG 2 cut(s) 728, 770
BsmAI GTCTC 2 cut(s) 140, 176
BsmFI GGGAC 2 cut(s) 87, 562
BsnI GGCC 1 cut(s) 459
Bso31I GGTCTC 1 cut(s) 176
BsoBI CYCGRG 1 cut(s) 545
Bsp119I TTCGAA 1 cut(s) 333
Bsp1286I GDGCHC 2 cut(s) 411, 649
Bsp143I GATC 1 cut(s) 557
Bsp1720I GCTNAGC 1 cut(s) 410
BspACI CCGC 1 cut(s) 809
BspANI GGCC 1 cut(s) 459
BspCNI CTCAG 4 cut(s) 307, 331, 423, 784
BspLI GGNNCC 2 cut(s) 345, 699
BspPI GGATC 1 cut(s) 565
BspT104I TTCGAA 1 cut(s) 333
BspTNI GGTCTC 1 cut(s) 176
BsrI ACTGG 2 cut(s) 58, 94
BssECI CCNNGG 1 cut(s) 545
BssMI GATC 1 cut(s) 557
Bst2UI CCWGG 1 cut(s) 30
Bst4CI ACNGT 1 cut(s) 644
Bst6I CTCTTC 2 cut(s) 138, 390
BstBI TTCGAA 1 cut(s) 333
BstC8I GCNNGC 3 cut(s) 525, 702, 723
BstDEI CTNAG 5 cut(s) 229, 315, 339, 410, 792
BstENI CCTNNNNNAGG 1 cut(s) 726
BstF5I GGATG 5 cut(s) 10, 376, 502, 635, 740
BstH2I RGCGCY 1 cut(s) 524
BstHHI GCGC 1 cut(s) 523
BstKTI GATC 1 cut(s) 560
BstMAI GTCTC 2 cut(s) 140, 176
BstMBI GATC 1 cut(s) 557
BstMWI GCNNNNNNNGC 3 cut(s) 445, 520, 569
BstNI CCWGG 1 cut(s) 30
BstSCI CCNGG 3 cut(s) 28, 544, 545
BstSFI CTRYAG 1 cut(s) 777
BstSLI GKGCMC 1 cut(s) 649
BstV1I GCAGC 2 cut(s) 550, 724
BstV2I GAAGAC 1 cut(s) 671
BstX2I RGATCY 1 cut(s) 557
BstYI RGATCY 1 cut(s) 557
Bsu36I CCTNAGG 2 cut(s) 315, 339
BsuRI GGCC 1 cut(s) 459
BtsCI GGATG 5 cut(s) 10, 376, 502, 635, 740
BtsI GCAGTG 1 cut(s) 250
BtsIMutI CAGTG 2 cut(s) 250, 649
Cac8I GCNNGC 3 cut(s) 525, 702, 723
CaiI CAGNNNCTG 1 cut(s) 785
CfoI GCGC 1 cut(s) 523
Cfr13I GGNCC 3 cut(s) 32, 773, 782
Cfr9I CCCGGG 1 cut(s) 545
CseI GACGC 2 cut(s) 84, 635
CsiI ACCWGGT 1 cut(s) 28
Csp6I GTAC 2 cut(s) 732, 756
CviAII CATG 1 cut(s) 269
CviJI RGCY 6 cut(s) 62, 299, 350, 409, 459, 700
CviKI_1 RGCY 6 cut(s) 62, 299, 350, 409, 459, 700
CviQI GTAC 2 cut(s) 732, 756
DdeI CTNAG 5 cut(s) 229, 315, 339, 410, 792
DpnI GATC 1 cut(s) 559
DpnII GATC 1 cut(s) 557
DraIII CACNNNGTG 1 cut(s) 41
Eam1104I CTCTTC 2 cut(s) 138, 390
EarI CTCTTC 2 cut(s) 138, 390
Eco24I GRGCYC 1 cut(s) 411
Eco31I GGTCTC 1 cut(s) 176
Eco47I GGWCC 3 cut(s) 32, 773, 782
Eco47III AGCGCT 1 cut(s) 522
Eco81I CCTNAGG 2 cut(s) 315, 339
Eco88I CYCGRG 1 cut(s) 545
EcoNI CCTNNNNNAGG 1 cut(s) 726
EcoO109I RGGNCCY 1 cut(s) 782
EcoRI GAATTC 1 cut(s) 329
EcoRII CCWGG 1 cut(s) 28
EcoT38I GRGCYC 1 cut(s) 411
FaeI CATG 1 cut(s) 272
FaiI YATR 2 cut(s) 270, 494
FaqI GGGAC 2 cut(s) 87, 562
FatI CATG 1 cut(s) 268
Fnu4HI GCNGC 2 cut(s) 564, 738
FokI GGATG 5 cut(s) 17, 383, 509, 622, 727
FriOI GRGCYC 1 cut(s) 411
Fsp4HI GCNGC 2 cut(s) 564, 738
GlaI GCGC 1 cut(s) 522
GluI GCNGC 2 cut(s) 564, 738
GsuI CTGGAG 1 cut(s) 339
HaeII RGCGCY 1 cut(s) 524
HaeIII GGCC 1 cut(s) 459
HapII CCGG 1 cut(s) 546
HgaI GACGC 2 cut(s) 84, 635
HhaI GCGC 1 cut(s) 523
Hin1II CATG 1 cut(s) 272
Hin6I GCGC 1 cut(s) 521
HinP1I GCGC 1 cut(s) 521
HinfI GANTC 1 cut(s) 467
HpaII CCGG 1 cut(s) 546
HphI GGTGA 6 cut(s) 104, 200, 266, 274, 632, 673
Hpy166II GTNNAC 2 cut(s) 35, 647
Hpy188I TCNGA 4 cut(s) 118, 472, 709, 793
Hpy188III TCNNGA 5 cut(s) 41, 314, 356, 420, 555
Hpy8I GTNNAC 2 cut(s) 35, 647
Hpy99I CGWCG 1 cut(s) 488
HpyAV CCTTC 5 cut(s) 40, 200, 244, 592, 652
HpyCH4III ACNGT 1 cut(s) 644
HpyCH4V TGCA 5 cut(s) 476, 527, 647, 725, 740
HpyF10VI GCNNNNNNNGC 3 cut(s) 445, 520, 569
HpyF3I CTNAG 5 cut(s) 229, 315, 339, 410, 792
Hsp92II CATG 1 cut(s) 272
HspAI GCGC 1 cut(s) 521
Kzo9I GATC 1 cut(s) 557
LmnI GCTCC 3 cut(s) 265, 355, 697
Lsp1109I GCAGC 2 cut(s) 550, 724
LweI GCATC 3 cut(s) 463, 644, 749
MabI ACCWGGT 1 cut(s) 28
MalI GATC 1 cut(s) 559
MboI GATC 1 cut(s) 557
MflI RGATCY 1 cut(s) 557
MhlI GDGCHC 2 cut(s) 411, 649
MluCI AATT 6 cut(s) 12, 109, 329, 360, 611, 801
MmeI TCCRAC 1 cut(s) 663
MseI TTAA 4 cut(s) 309, 363, 540, 614
MspI CCGG 1 cut(s) 546
MspR9I CCNGG 3 cut(s) 30, 546, 547
MvaI CCWGG 1 cut(s) 30
MwoI GCNNNNNNNGC 3 cut(s) 445, 520, 569
NciI CCSGG 2 cut(s) 546, 547
NdeII GATC 1 cut(s) 557
NlaIII CATG 1 cut(s) 272
NlaIV GGNNCC 2 cut(s) 345, 699
NspV TTCGAA 1 cut(s) 333
PfeI GAWTC 1 cut(s) 467
PflFI GACNNNGTC 1 cut(s) 168
PflMI CCANNNNNTGG 1 cut(s) 770
PkrI GCNGC 2 cut(s) 565, 739
PpuMI RGGWCCY 1 cut(s) 782
PshBI ATTAAT 1 cut(s) 309
Psp5II RGGWCCY 1 cut(s) 782
Psp6I CCWGG 1 cut(s) 28
PspGI CCWGG 1 cut(s) 28
PspN4I GGNNCC 2 cut(s) 345, 699
PspPI GGNCC 3 cut(s) 32, 773, 782
PspPPI RGGWCCY 1 cut(s) 782
PstNI CAGNNNCTG 1 cut(s) 785
PsuI RGATCY 1 cut(s) 557
PsyI GACNNNGTC 1 cut(s) 168
RsaI GTAC 2 cut(s) 733, 757
RsaNI GTAC 2 cut(s) 732, 756
SaqAI TTAA 4 cut(s) 309, 363, 540, 614
SatI GCNGC 2 cut(s) 564, 738
Sau3AI GATC 1 cut(s) 557
Sau96I GGNCC 3 cut(s) 32, 773, 782
ScrFI CCNGG 3 cut(s) 30, 546, 547
SduI GDGCHC 2 cut(s) 411, 649
SexAI ACCWGGT 1 cut(s) 28
SfaNI GCATC 3 cut(s) 463, 644, 749
SfcI CTRYAG 1 cut(s) 777
SfuI TTCGAA 1 cut(s) 333
SinI GGWCC 3 cut(s) 32, 773, 782
SmaI CCCGGG 1 cut(s) 547
SmlI CTYRAG 1 cut(s) 516
SmoI CTYRAG 1 cut(s) 516
Sse9I AATT 6 cut(s) 12, 109, 329, 360, 611, 801
SsiI CCGC 1 cut(s) 809
SspI AATATT 1 cut(s) 279
StyD4I CCNGG 3 cut(s) 28, 544, 545
TaaI ACNGT 1 cut(s) 644
TaqI TCGA 2 cut(s) 333, 746
TaqII GACCGA 1 cut(s) 683
TasI AATT 6 cut(s) 12, 109, 329, 360, 611, 801
TfiI GAWTC 1 cut(s) 467
Tru1I TTAA 4 cut(s) 309, 363, 540, 614
Tru9I TTAA 4 cut(s) 309, 363, 540, 614
TscAI CASTG 2 cut(s) 250, 649
TseI GCWGC 2 cut(s) 563, 737
TspDTI ATGAA 6 cut(s) 24, 219, 231, 285, 309, 408
TspMI CCCGGG 1 cut(s) 545
TspRI CASTG 2 cut(s) 250, 649
Tth111I GACNNNGTC 1 cut(s) 168
Van91I CCANNNNNTGG 1 cut(s) 770
VneI GTGCAC 1 cut(s) 645
VpaK11BI GGWCC 3 cut(s) 32, 773, 782
VspI ATTAAT 1 cut(s) 309
XagI CCTNNNNNAGG 1 cut(s) 726
XapI RAATTY 2 cut(s) 109, 329
XmaI CCCGGG 1 cut(s) 545
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.