Rh6CG326300

Transcription initiation factor IIF subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0001724
Physical Location & Seq
Reverse (-)
51326256 .. 51328540
2285 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG326300.1

Sequence Viewer

Length: 561 bp
ATGAGAAAACGATATGATGATGATGCCGAGGCATTTAGCTGTGCACCTCCCCAGAAGTTGAAGACTGAAAATGATAGATCTGAGTACCATGGTCATCTTCAAGAATCGTTTTCAGCTTACTACCTACTAATGCAAGAAGGAGAGGAGATTTCAGATGTTCCTGCTGGTTCCTGGTTCAACTTTGACAAAGTTCAACAGTATAATAAGCAACTTGCTTTGGCAGAAACCGAAGCGAAGATGATCCATAAGAGAAAACGATATGATGATGATGCCGAGGCCTTTAGCTGTGCACCTTCCAAGAAGTTGAAGACCGAAAATGATCAAAAACCTTCCATAGAAGAAGAGCCTGCATCTGCTAGTCCTATCAGTGAGGAAGAAATCAGGGCTGTTTTTGAGAAAGAACAAGCAATTTCCTTACAAGACCTTGTTGACCACTATCCAGGAAGAGTCGAATCGAATGAGGAGCTGAAGGCTTTAATAGCAGGGTTAAGGAGTATGTCTTACAAAATACTATATGTAAGAGACCGAAAGACGATGAAACTGGTTACAATCTTGTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005515 GO:0008022
KEGG Pathways
Metabolic & Signaling
ko03022 map03022
Pfam Domains
Protein Families
TFIIF_alpha

Protein Analysis

186

Amino Acids

21.51

Weight (kDa)

5.16

Isoelectric Point (pI)

75.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TFIIF_alpha PF05793 16 - 86 2.6e-10 Transcription initiation factor IIF, alpha subunit (TFIIF-alpha)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 235
AcuI CTGAAG 1 cut(s) 488
AfaI GTAC 1 cut(s) 86
AgsI TTSAA 5 cut(s) 61, 101, 178, 194, 307
AjnI CCWGG 2 cut(s) 170, 439
AluBI AGCT 4 cut(s) 39, 116, 285, 466
AluI AGCT 4 cut(s) 39, 116, 285, 466
Alw21I GWGCWC 2 cut(s) 46, 292
Alw26I GTCTC 1 cut(s) 516
Alw44I GTGCAC 2 cut(s) 42, 288
AlwI GGATC 1 cut(s) 235
AoxI GGCC 1 cut(s) 276
ApaLI GTGCAC 2 cut(s) 42, 288
BaeGI GKGCMC 2 cut(s) 46, 292
BbsI GAAGAC 2 cut(s) 68, 314
Bbv12I GWGCWC 2 cut(s) 46, 292
BciT130I CCWGG 2 cut(s) 172, 441
BclI TGATCA 1 cut(s) 319
BcoDI GTCTC 1 cut(s) 516
BfaI CTAG 1 cut(s) 357
BglII AGATCT 1 cut(s) 77
Bme1390I CCNGG 2 cut(s) 172, 441
BmiI GGNNCC 1 cut(s) 169
BmrFI CCNGG 2 cut(s) 172, 441
BmsI GCATC 3 cut(s) 13, 259, 359
BpiI GAAGAC 2 cut(s) 68, 314
BsaI GGTCTC 1 cut(s) 516
BsaJI CCNNGG 3 cut(s) 27, 88, 273
Bse1I ACTGG 1 cut(s) 546
BseBI CCWGG 2 cut(s) 172, 441
BseDI CCNNGG 3 cut(s) 27, 88, 273
BseMII CTCAG 1 cut(s) 72
BseNI ACTGG 1 cut(s) 546
BseRI GAGGAG 2 cut(s) 158, 476
BseSI GKGCMC 2 cut(s) 46, 292
BshFI GGCC 1 cut(s) 278
BsiHKAI GWGCWC 2 cut(s) 46, 292
BsmAI GTCTC 1 cut(s) 516
BsnI GGCC 1 cut(s) 278
Bso31I GGTCTC 1 cut(s) 516
Bsp1286I GDGCHC 2 cut(s) 46, 292
Bsp143I GATC 3 cut(s) 77, 240, 319
Bsp19I CCATGG 1 cut(s) 88
BspANI GGCC 1 cut(s) 278
BspCNI CTCAG 1 cut(s) 73
BspLI GGNNCC 1 cut(s) 169
BspPI GGATC 1 cut(s) 235
BspQI GCTCTTC 1 cut(s) 336
BspTNI GGTCTC 1 cut(s) 516
BsrI ACTGG 1 cut(s) 546
BssECI CCNNGG 3 cut(s) 27, 88, 273
BssMI GATC 3 cut(s) 77, 240, 319
BssT1I CCWWGG 1 cut(s) 88
Bst2UI CCWGG 2 cut(s) 172, 441
Bst4CI ACNGT 1 cut(s) 198
Bst6I CTCTTC 2 cut(s) 336, 439
BstC8I GCNNGC 1 cut(s) 348
BstDEI CTNAG 1 cut(s) 81
BstDSI CCRYGG 1 cut(s) 88
BstKTI GATC 3 cut(s) 80, 243, 322
BstMAI GTCTC 1 cut(s) 516
BstMBI GATC 3 cut(s) 77, 240, 319
BstMWI GCNNNNNNNGC 1 cut(s) 479
BstNI CCWGG 2 cut(s) 172, 441
BstSCI CCNGG 2 cut(s) 170, 439
BstSLI GKGCMC 2 cut(s) 46, 292
BstV2I GAAGAC 2 cut(s) 68, 314
BstX2I RGATCY 1 cut(s) 77
BstYI RGATCY 1 cut(s) 77
BsuRI GGCC 1 cut(s) 278
BtgI CCRYGG 1 cut(s) 88
BtsIMutI CAGTG 1 cut(s) 373
Cac8I GCNNGC 1 cut(s) 348
Csp6I GTAC 1 cut(s) 85
CviAII CATG 1 cut(s) 89
CviJI RGCY 8 cut(s) 39, 116, 278, 285, 346, 386, 466, 473
CviKI_1 RGCY 8 cut(s) 39, 116, 278, 285, 346, 386, 466, 473
CviQI GTAC 1 cut(s) 85
DdeI CTNAG 1 cut(s) 81
DpnI GATC 3 cut(s) 79, 242, 321
DpnII GATC 3 cut(s) 77, 240, 319
Eam1104I CTCTTC 2 cut(s) 336, 439
EarI CTCTTC 2 cut(s) 336, 439
Eco130I CCWWGG 1 cut(s) 88
Eco147I AGGCCT 1 cut(s) 278
Eco31I GGTCTC 1 cut(s) 516
Eco57I CTGAAG 1 cut(s) 488
EcoRII CCWGG 2 cut(s) 170, 439
EcoT14I CCWWGG 1 cut(s) 88
ErhI CCWWGG 1 cut(s) 88
FaeI CATG 1 cut(s) 92
FaiI YATR 9 cut(s) 15, 90, 201, 246, 261, 335, 497, 514, 516
FatI CATG 1 cut(s) 88
FbaI TGATCA 1 cut(s) 319
FspBI CTAG 1 cut(s) 357
HaeIII GGCC 1 cut(s) 278
Hin1II CATG 1 cut(s) 92
HincII GTYRAC 1 cut(s) 430
HindII GTYRAC 1 cut(s) 430
HinfI GANTC 3 cut(s) 104, 447, 452
Hpy166II GTNNAC 3 cut(s) 44, 290, 430
Hpy188I TCNGA 2 cut(s) 82, 154
Hpy188III TCNNGA 1 cut(s) 101
Hpy8I GTNNAC 3 cut(s) 44, 290, 430
HpyAV CCTTC 4 cut(s) 131, 303, 339, 463
HpyCH4III ACNGT 1 cut(s) 198
HpyCH4V TGCA 4 cut(s) 44, 133, 290, 350
HpyF10VI GCNNNNNNNGC 1 cut(s) 479
HpyF3I CTNAG 1 cut(s) 81
Hsp92II CATG 1 cut(s) 92
Ksp22I TGATCA 1 cut(s) 319
Kzo9I GATC 3 cut(s) 77, 240, 319
LguI GCTCTTC 1 cut(s) 336
LmnI GCTCC 1 cut(s) 463
LweI GCATC 3 cut(s) 13, 259, 359
MaeI CTAG 1 cut(s) 357
MaeIII GTNAC 1 cut(s) 544
MalI GATC 3 cut(s) 79, 242, 321
MboI GATC 3 cut(s) 77, 240, 319
MboII GAAGA 8 cut(s) 73, 89, 247, 319, 350, 353, 386, 456
MflI RGATCY 1 cut(s) 77
MhlI GDGCHC 2 cut(s) 46, 292
MluCI AATT 1 cut(s) 408
MlyI GAGTC 1 cut(s) 456
MnlI CCTC 6 cut(s) 22, 57, 136, 268, 364, 454
MseI TTAA 2 cut(s) 476, 488
MspR9I CCNGG 2 cut(s) 172, 441
MvaI CCWGG 2 cut(s) 172, 441
MwoI GCNNNNNNNGC 1 cut(s) 479
NcoI CCATGG 1 cut(s) 88
NdeII GATC 3 cut(s) 77, 240, 319
NlaIII CATG 1 cut(s) 92
NlaIV GGNNCC 1 cut(s) 169
NmeAIII GCCGAG 2 cut(s) 52, 298
PceI AGGCCT 1 cut(s) 278
PciSI GCTCTTC 1 cut(s) 336
PfeI GAWTC 2 cut(s) 104, 452
PfoI TCCNGGA 1 cut(s) 439
PleI GAGTC 1 cut(s) 455
PpsI GAGTC 1 cut(s) 455
Psp6I CCWGG 2 cut(s) 170, 439
PspGI CCWGG 2 cut(s) 170, 439
PspN4I GGNNCC 1 cut(s) 169
PsuI RGATCY 1 cut(s) 77
RsaI GTAC 1 cut(s) 86
RsaNI GTAC 1 cut(s) 85
SapI GCTCTTC 1 cut(s) 336
SaqAI TTAA 2 cut(s) 476, 488
Sau3AI GATC 3 cut(s) 77, 240, 319
SchI GAGTC 1 cut(s) 456
ScrFI CCNGG 2 cut(s) 172, 441
SduI GDGCHC 2 cut(s) 46, 292
SetI ASST 9 cut(s) 41, 49, 118, 126, 287, 295, 331, 426, 468
SfaNI GCATC 3 cut(s) 13, 259, 359
Sse9I AATT 1 cut(s) 408
SseBI AGGCCT 1 cut(s) 278
SspMI CTAG 1 cut(s) 357
StuI AGGCCT 1 cut(s) 278
StyD4I CCNGG 2 cut(s) 170, 439
StyI CCWWGG 1 cut(s) 88
TaaI ACNGT 1 cut(s) 198
TaqI TCGA 2 cut(s) 450, 455
TaqII GACCGA 2 cut(s) 326, 540
TasI AATT 1 cut(s) 408
TfiI GAWTC 2 cut(s) 104, 452
Tru1I TTAA 2 cut(s) 476, 488
Tru9I TTAA 2 cut(s) 476, 488
TscAI CASTG 1 cut(s) 373
TspDTI ATGAA 1 cut(s) 551
TspRI CASTG 1 cut(s) 373
VneI GTGCAC 2 cut(s) 42, 288
XspI CTAG 1 cut(s) 357
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.