FvH4_3g01042

Transcription initiation factor IIF subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0001724
Physical Location & Seq
Forward (+)
522764 .. 527661
4898 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g01042.t1

Sequence Viewer

Length: 1653 bp
ATGTCGTCGGACTTGTTGCTGAAGCCGTCGTGCGGCGGTTGCGGATCGACTTCCGATTTGTACGGAAGCAACTGTAAGCACATGACGCTGTGCTGCGCGTGCGGCAAAACCATGGCGGAGAATAAAGCCAAATGCTACGACTGCGGCGCCGTCGTCACTCGCTTGATTCGAGAGTATAATGTTAGGGCAAGCACTGCCAGCGAGAAGAGCTACTTCATTGGTAGGTTTGTGACGGGGTTGCCCAACTTTTCGAAGAAGAAGAGTGCCGAAAACAAATGGACTCTTCGGAAGGATCTTTCGGATCCGAGGAGGAGTAAGGATAAGGATGGTCAGGACCAGAAGAAATATAAGGATGTTCGGCCGCCTACTGAGGCATCTCGGGAGAAGCTCAAAAACAAACCATGGGTGTTGGAGGATGAAACTGGCCAGTCTCAGTACAATGGACAACTTGAAGGTGCACAGTCAGCAATCTACTACCTACTAATGATGCAAGGAAAAGAGTTTTCTGCTATTCCCGCCGGTTCTTGGTTCAACTTTAACAAGGTTGCACAATATAAGCAACTTACTCTAGAAGAAGCAGAAGAGAAAATGAACAATAGGAAAAAGACTCAAGATGGATATGAAAGATGGATGATGAAATCCGGAACAAATGGACCTGGTCCACTTGGTGAGAAGGAACCTAGTGTGGCTAGTGCCAAAGGACGCAAAAAAATGACTGGTGATGATGATGAGGGAAACTTCTCTGATAAAGGGGAGGAGGATGAGGATGAAGAGACAGCAAGGAAAAATAGACTTGGTCTCAACAAAAAAGGTGGTGATGATGAGGATGATGAAGGTGGAAATGAAGATGAGGACCTAGATGATGATAGCAGTGAGAAGGGTGATGATTGGGAGCACGAAGAAATATTCACTGATGATGATGAAGCTGTTGGTATAAATCCTGAGGAGAGAGAAGAATTCGAACCTGAGGTTCCAGCTCCTCCAGAAATTAAGCAGGACGATGATGATGATGATGCTAATGAAGGGGAGGGTGGGCTGAGCAAATCAGGAAAAGAGTTGAAGAAGCTACTTGGGCGAAGTGGGGGCCTGAATGATTCAGATGGAGAGGACGATGACGACGACGACGACGATATGGATGATGATATTGCCTTGCCTCAACCGCTGGCTCCAAAGCAGAAGGATGCACCCAAACAGGAACCAGTTGACAATAGTCCTGTTAAACCAGGGGCTTCTGGATCTGCTGCTAAAGCAAACCCATCTGCCTCCAAGTCATCTAAGGGAAAGAGAAAATCGAATGGTGATGATAATAAAACACCCAACAGTGCTCCACCCAAGAAAGTGAAGACCGAAAATGAGCAAAAATCAGTCAAAGAGGAGCCTGCCCCTGATAGTACTGTGCCTGCAAAAGGTACTGCTGCACCCTCGAAACCGGGTTCGACACCATCCGGTGGTCGTACAGGTCCGGTAACTGAGGAAGAAATTAGGGCAGTTTTGATGCAAAAGTCACCAATAACCACACGTGATCTTGTTAATAACTTCCCTGGACGACTCAAAACCAAAGAGGAGAAGGATGCTTTTGCAGCAATCCTGAGGATAATGTCTAGGATACAGAAGAACAACGGGATGAGCTATGTTGTGTTGAGGGAGAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005515 GO:0008022
KEGG Pathways
Metabolic & Signaling
ko03022 map03022
Pfam Domains
Protein Families
TFIIF_alpha

Protein Analysis

551

Amino Acids

60.36

Weight (kDa)

4.88

Isoelectric Point (pI)

47.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TFIIF_alpha PF05793 48 - 548 1.1e-122 Transcription initiation factor IIF, alpha subunit (TFIIF-alpha)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 146
AccB7I CCANNNNNTGG 1 cut(s) 1448
AccII CGCG 1 cut(s) 98
AccIII TCCGGA 1 cut(s) 641
AciI CCGC 9 cut(s) 33, 36, 42, 102, 116, 144, 362, 516, 1160
AclWI GGATC 5 cut(s) 52, 296, 300, 309, 1243
AcoI YGGCCR 2 cut(s) 359, 424
AcsI RAATTY 1 cut(s) 956
AcuI CTGAAG 1 cut(s) 41
AcvI CACGTG 1 cut(s) 1520
AcyI GRCGYC 1 cut(s) 147
AdeI CACNNNGTG 1 cut(s) 668
AfaI GTAC 5 cut(s) 62, 437, 1393, 1411, 1456
AfiI CCNNNNNNNGG 4 cut(s) 32, 525, 1406, 1448
AflIII ACRYGT 1 cut(s) 1517
AgsI TTSAA 3 cut(s) 452, 532, 1060
AjnI CCWGG 3 cut(s) 655, 1222, 1540
AluBI AGCT 6 cut(s) 210, 388, 926, 977, 1066, 1629
AluI AGCT 6 cut(s) 210, 388, 926, 977, 1066, 1629
Alw21I GWGCWC 3 cut(s) 460, 897, 1327
Alw26I GTCTC 3 cut(s) 435, 767, 803
Alw44I GTGCAC 1 cut(s) 456
AlwI GGATC 5 cut(s) 52, 296, 300, 309, 1243
Ama87I CYCGRG 1 cut(s) 378
Aor13HI TCCGGA 1 cut(s) 641
AoxI GGCC 3 cut(s) 359, 424, 1084
ApaLI GTGCAC 1 cut(s) 456
ApeKI GCWGC 4 cut(s) 93, 1241, 1415, 1580
ApoI RAATTY 1 cut(s) 956
ArsI GACNNNNNNTTYG 2 cut(s) 40, 72
AspLEI GCGC 2 cut(s) 98, 149
AspS9I GGNCC 6 cut(s) 334, 653, 659, 853, 1084, 1460
AsuC2I CCSGG 1 cut(s) 1431
AsuHPI GGTGA 6 cut(s) 680, 731, 827, 893, 1310, 1497
AsuII TTCGAA 2 cut(s) 251, 960
AvaI CYCGRG 1 cut(s) 378
AvaII GGWCC 5 cut(s) 334, 653, 659, 853, 1460
AxyI CCTNAGG 3 cut(s) 942, 966, 1589
BaeGI GKGCMC 1 cut(s) 460
BalI TGGCCA 1 cut(s) 426
BamHI GGATCC 1 cut(s) 301
BanI GGYRCC 1 cut(s) 146
BbrPI CACGTG 1 cut(s) 1520
BbsI GAAGAC 1 cut(s) 1349
Bbv12I GWGCWC 3 cut(s) 460, 897, 1327
BbvI GCAGC 4 cut(s) 80, 1228, 1402, 1592
BccI CCATC 6 cut(s) 320, 608, 621, 1094, 1264, 1450
BceAI ACGGC 2 cut(s) 10, 134
BciT130I CCWGG 3 cut(s) 657, 1224, 1542
BciVI GTATCC 1 cut(s) 1599
BcnI CCSGG 1 cut(s) 1431
BcoDI GTCTC 3 cut(s) 435, 767, 803
BfaI CTAG 5 cut(s) 569, 681, 690, 857, 1602
BfoI RGCGCY 1 cut(s) 150
BfuI GTATCC 1 cut(s) 1599
BisI GCNGC 8 cut(s) 34, 94, 103, 145, 362, 1242, 1416, 1581
BlpI GCTNAGC 1 cut(s) 1037
BlsI GCNGC 8 cut(s) 35, 95, 104, 146, 363, 1243, 1417, 1582
BmcAI AGTACT 1 cut(s) 1393
Bme1390I CCNGG 4 cut(s) 657, 1224, 1431, 1542
Bme18I GGWCC 5 cut(s) 334, 653, 659, 853, 1460
BmeT110I CYCGRG 1 cut(s) 378
BmgT120I GGNCC 6 cut(s) 334, 653, 659, 853, 1084, 1460
BmiI GGNNCC 8 cut(s) 148, 303, 678, 972, 1085, 1167, 1197, 1377
BmrFI CCNGG 4 cut(s) 657, 1224, 1431, 1542
BmsI GCATC 6 cut(s) 383, 477, 1003, 1171, 1485, 1561
BoxI GACNNNNGTC 1 cut(s) 1209
BpiI GAAGAC 1 cut(s) 1349
BpmI CTGGAG 1 cut(s) 966
Bpu1102I GCTNAGC 1 cut(s) 1037
Bpu14I TTCGAA 2 cut(s) 251, 960
BpuEI CTTGAG 1 cut(s) 594
BpuMI CCSGG 1 cut(s) 1431
BsaAI YACGTR 1 cut(s) 1520
BsaHI GRCGYC 1 cut(s) 147
BsaI GGTCTC 1 cut(s) 803
BsaJI CCNNGG 5 cut(s) 111, 305, 401, 1223, 1540
BsaWI WCCGGW 3 cut(s) 641, 1445, 1462
Bsc4I CCNNNNNNNGG 4 cut(s) 32, 525, 1406, 1448
Bse118I RCCGGY 1 cut(s) 518
Bse1I ACTGG 4 cut(s) 427, 427, 721, 1199
Bse21I CCTNAGG 3 cut(s) 942, 966, 1589
BseAI TCCGGA 1 cut(s) 641
BseBI CCWGG 3 cut(s) 657, 1224, 1542
BseDI CCNNGG 5 cut(s) 111, 305, 401, 1223, 1540
BseLI CCNNNNNNNGG 4 cut(s) 32, 525, 1406, 1448
BseMII CTCAG 7 cut(s) 360, 446, 933, 957, 1028, 1461, 1580
BseNI ACTGG 4 cut(s) 427, 427, 721, 1199
BseRI GAGGAG 7 cut(s) 322, 325, 770, 959, 969, 1388, 1577
BseSI GKGCMC 1 cut(s) 460
BseX3I CGGCCG 1 cut(s) 359
BseXI GCAGC 4 cut(s) 80, 1228, 1402, 1592
BsgI GTGCAG 1 cut(s) 1401
Bsh1236I CGCG 1 cut(s) 98
Bsh1285I CGRYCG 1 cut(s) 362
BshFI GGCC 3 cut(s) 361, 426, 1086
BshNI GGYRCC 1 cut(s) 146
BsiEI CGRYCG 1 cut(s) 362
BsiHKAI GWGCWC 3 cut(s) 460, 897, 1327
BsiHKCI CYCGRG 1 cut(s) 378
BsiSI CCGG 5 cut(s) 519, 642, 1430, 1446, 1463
BslI CCNNNNNNNGG 4 cut(s) 32, 525, 1406, 1448
BsmAI GTCTC 3 cut(s) 435, 767, 803
BsnI GGCC 3 cut(s) 361, 426, 1086
Bso31I GGTCTC 1 cut(s) 803
BsoBI CYCGRG 1 cut(s) 378
Bsp119I TTCGAA 2 cut(s) 251, 960
Bsp1286I GDGCHC 3 cut(s) 460, 897, 1327
Bsp13I TCCGGA 1 cut(s) 641
Bsp143I GATC 5 cut(s) 44, 292, 301, 1235, 1522
Bsp1720I GCTNAGC 1 cut(s) 1037
Bsp19I CCATGG 2 cut(s) 111, 401
BspACI CCGC 9 cut(s) 33, 36, 42, 102, 116, 144, 362, 516, 1160
BspANI GGCC 3 cut(s) 361, 426, 1086
BspCNI CTCAG 7 cut(s) 361, 445, 934, 958, 1029, 1462, 1581
BspEI TCCGGA 1 cut(s) 641
BspFNI CGCG 1 cut(s) 98
BspLI GGNNCC 8 cut(s) 148, 303, 678, 972, 1085, 1167, 1197, 1377
BspPI GGATC 5 cut(s) 52, 296, 300, 309, 1243
BspQI GCTCTTC 1 cut(s) 200
BspT104I TTCGAA 2 cut(s) 251, 960
BspT107I GGYRCC 1 cut(s) 146
BspTNI GGTCTC 1 cut(s) 803
BsrFI RCCGGY 1 cut(s) 518
BsrI ACTGG 4 cut(s) 427, 427, 721, 1199
BssAI RCCGGY 1 cut(s) 518
BssECI CCNNGG 5 cut(s) 111, 305, 401, 1223, 1540
BssMI GATC 5 cut(s) 44, 292, 301, 1235, 1522
BssNI GRCGYC 1 cut(s) 147
BssT1I CCWWGG 2 cut(s) 111, 401
Bst2UI CCWGG 3 cut(s) 657, 1224, 1542
Bst4CI ACNGT 4 cut(s) 74, 462, 1322, 1396
Bst6I CTCTTC 5 cut(s) 200, 254, 288, 576, 765
BstACI GRCGYC 1 cut(s) 147
BstAPI GCANNNNNTGC 1 cut(s) 194
BstBAI YACGTR 1 cut(s) 1520
BstBI TTCGAA 2 cut(s) 251, 960
BstC8I GCNNGC 6 cut(s) 100, 190, 199, 1164, 1380, 1401
BstDEI CTNAG 8 cut(s) 369, 432, 942, 966, 1037, 1275, 1470, 1589
BstDSI CCRYGG 2 cut(s) 111, 401
BstENI CCTNNNNNAGG 1 cut(s) 1404
BstFNI CGCG 1 cut(s) 98
BstH2I RGCGCY 1 cut(s) 150
BstHHI GCGC 2 cut(s) 98, 149
BstKTI GATC 5 cut(s) 47, 295, 304, 1238, 1525
BstMAI GTCTC 3 cut(s) 435, 767, 803
BstMBI GATC 5 cut(s) 44, 292, 301, 1235, 1522
BstMCI CGRYCG 1 cut(s) 362
BstNI CCWGG 3 cut(s) 657, 1224, 1542
BstPAI GACNNNNGTC 1 cut(s) 1209
BstSCI CCNGG 4 cut(s) 655, 1222, 1429, 1540
BstSLI GKGCMC 1 cut(s) 460
BstUI CGCG 1 cut(s) 98
BstV1I GCAGC 4 cut(s) 80, 1228, 1402, 1592
BstV2I GAAGAC 1 cut(s) 1349
BstX2I RGATCY 3 cut(s) 292, 301, 1235
BstYI RGATCY 3 cut(s) 292, 301, 1235
BstZI CGGCCG 1 cut(s) 359
Bsu36I CCTNAGG 3 cut(s) 942, 966, 1589
BsuI GTATCC 1 cut(s) 1599
BsuRI GGCC 3 cut(s) 361, 426, 1086
BtgI CCRYGG 2 cut(s) 111, 401
BtsI GCAGTG 2 cut(s) 192, 877
BtsIMutI CAGTG 4 cut(s) 192, 877, 909, 1327
Cac8I GCNNGC 6 cut(s) 100, 190, 199, 1164, 1380, 1401
CfoI GCGC 2 cut(s) 98, 149
Cfr10I RCCGGY 1 cut(s) 518
Cfr13I GGNCC 6 cut(s) 334, 653, 659, 853, 1084, 1460
CseI GACGC 2 cut(s) 94, 711
CsiI ACCWGGT 1 cut(s) 655
Csp6I GTAC 5 cut(s) 61, 436, 1392, 1410, 1455
CspCI CAANNNNNGTGG 2 cut(s) 793, 828
CviAII CATG 3 cut(s) 82, 112, 402
CviQI GTAC 5 cut(s) 61, 436, 1392, 1410, 1455
DdeI CTNAG 8 cut(s) 369, 432, 942, 966, 1037, 1275, 1470, 1589
DinI GGCGCC 1 cut(s) 148
DpnI GATC 5 cut(s) 46, 294, 303, 1237, 1524
DpnII GATC 5 cut(s) 44, 292, 301, 1235, 1522
DraIII CACNNNGTG 1 cut(s) 668
EaeI YGGCCR 2 cut(s) 359, 424
EagI CGGCCG 1 cut(s) 359
Eam1104I CTCTTC 5 cut(s) 200, 254, 288, 576, 765
EarI CTCTTC 5 cut(s) 200, 254, 288, 576, 765
EciI GGCGGA 1 cut(s) 131
EclXI CGGCCG 1 cut(s) 359
Eco130I CCWWGG 2 cut(s) 111, 401
Eco31I GGTCTC 1 cut(s) 803
Eco47I GGWCC 5 cut(s) 334, 653, 659, 853, 1460
Eco52I CGGCCG 1 cut(s) 359
Eco57I CTGAAG 1 cut(s) 41
Eco72I CACGTG 1 cut(s) 1520
Eco81I CCTNAGG 3 cut(s) 942, 966, 1589
Eco88I CYCGRG 1 cut(s) 378
EcoNI CCTNNNNNAGG 1 cut(s) 1404
EcoO109I RGGNCCY 2 cut(s) 853, 1084
EcoRI GAATTC 1 cut(s) 956
EcoRII CCWGG 3 cut(s) 655, 1222, 1540
EcoT14I CCWWGG 2 cut(s) 111, 401
EgeI GGCGCC 1 cut(s) 148
EheI GGCGCC 1 cut(s) 148
ErhI CCWWGG 2 cut(s) 111, 401
FaeI CATG 3 cut(s) 85, 115, 405
FalI AAGNNNNNCTT 2 cut(s) 197, 229
FatI CATG 3 cut(s) 81, 111, 401
FauI CCCGC 1 cut(s) 523
Fnu4HI GCNGC 8 cut(s) 34, 94, 103, 145, 362, 1242, 1416, 1581
Fsp4HI GCNGC 8 cut(s) 34, 94, 103, 145, 362, 1242, 1416, 1581
FspBI CTAG 5 cut(s) 569, 681, 690, 857, 1602
GlaI GCGC 2 cut(s) 97, 148
GluI GCNGC 8 cut(s) 34, 94, 103, 145, 362, 1242, 1416, 1581
GsuI CTGGAG 1 cut(s) 966
HaeII RGCGCY 1 cut(s) 150
HaeIII GGCC 3 cut(s) 361, 426, 1086
HapII CCGG 5 cut(s) 519, 642, 1430, 1446, 1463
HgaI GACGC 2 cut(s) 94, 711
HhaI GCGC 2 cut(s) 98, 149
Hin1I GRCGYC 1 cut(s) 147
Hin1II CATG 3 cut(s) 85, 115, 405
Hin6I GCGC 2 cut(s) 96, 147
HinP1I GCGC 2 cut(s) 96, 147
HincII GTYRAC 1 cut(s) 1204
HindII GTYRAC 1 cut(s) 1204
HinfI GANTC 5 cut(s) 166, 280, 607, 1094, 1548
HpaII CCGG 5 cut(s) 519, 642, 1430, 1446, 1463
HphI GGTGA 6 cut(s) 680, 731, 827, 893, 1310, 1497
Hpy166II GTNNAC 3 cut(s) 458, 662, 1204
Hpy188I TCNGA 7 cut(s) 10, 55, 288, 301, 306, 745, 1099
Hpy8I GTNNAC 3 cut(s) 458, 662, 1204
Hpy99I CGWCG 7 cut(s) 10, 31, 155, 1121, 1124, 1127, 1130
HpyAV CCTTC 8 cut(s) 283, 446, 667, 827, 871, 1016, 1171, 1561
HpyCH4III ACNGT 4 cut(s) 74, 462, 1322, 1396
HpyCH4IV ACGT 1 cut(s) 1519
HpyCH4V TGCA 8 cut(s) 458, 490, 548, 1184, 1403, 1418, 1498, 1580
HpyF3I CTNAG 8 cut(s) 369, 432, 942, 966, 1037, 1275, 1470, 1589
HpySE526I ACGT 1 cut(s) 1519
Hsp92I GRCGYC 1 cut(s) 147
Hsp92II CATG 3 cut(s) 85, 115, 405
HspAI GCGC 2 cut(s) 96, 147
KasI GGCGCC 1 cut(s) 146
Kpn2I TCCGGA 1 cut(s) 641
Kzo9I GATC 5 cut(s) 44, 292, 301, 1235, 1522
LguI GCTCTTC 1 cut(s) 200
LmnI GCTCC 5 cut(s) 892, 982, 1171, 1330, 1375
Lsp1109I GCAGC 4 cut(s) 80, 1228, 1402, 1592
LweI GCATC 6 cut(s) 383, 477, 1003, 1171, 1485, 1561
MabI ACCWGGT 1 cut(s) 655
MaeI CTAG 5 cut(s) 569, 681, 690, 857, 1602
MaeII ACGT 1 cut(s) 1519
MaeIII GTNAC 4 cut(s) 154, 229, 1465, 1503
MalI GATC 5 cut(s) 46, 294, 303, 1237, 1524
MboI GATC 5 cut(s) 44, 292, 301, 1235, 1522
MflI RGATCY 3 cut(s) 292, 301, 1235
MhlI GDGCHC 3 cut(s) 460, 897, 1327
MlsI TGGCCA 1 cut(s) 426
MluCI AATT 3 cut(s) 956, 987, 1479
MluNI TGGCCA 1 cut(s) 426
Mly113I GGCGCC 1 cut(s) 147
MlyI GAGTC 3 cut(s) 274, 601, 1542
MmeI TCCRAC 1 cut(s) 390
Mox20I TGGCCA 1 cut(s) 426
MroI TCCGGA 1 cut(s) 641
MscI TGGCCA 1 cut(s) 426
MseI TTAA 4 cut(s) 537, 990, 1218, 1530
Msp20I TGGCCA 1 cut(s) 426
MspA1I CMGCKG 1 cut(s) 1162
MspI CCGG 5 cut(s) 519, 642, 1430, 1446, 1463
MspR9I CCNGG 4 cut(s) 657, 1224, 1431, 1542
MvaI CCWGG 3 cut(s) 657, 1224, 1542
MvnI CGCG 1 cut(s) 98
NarI GGCGCC 1 cut(s) 147
NciI CCSGG 1 cut(s) 1431
NcoI CCATGG 2 cut(s) 111, 401
NdeII GATC 5 cut(s) 44, 292, 301, 1235, 1522
NlaIII CATG 3 cut(s) 85, 115, 405
NlaIV GGNNCC 8 cut(s) 148, 303, 678, 972, 1085, 1167, 1197, 1377
NmuCI GTSAC 3 cut(s) 154, 229, 1503
NspV TTCGAA 2 cut(s) 251, 960
PciSI GCTCTTC 1 cut(s) 200
PcsI WCGNNNNNNNCGW 4 cut(s) 166, 1116, 1122, 1125
PfeI GAWTC 2 cut(s) 166, 1094
PflFI GACNNNGTC 2 cut(s) 657, 795
PflMI CCANNNNNTGG 1 cut(s) 1448
PkrI GCNGC 8 cut(s) 35, 95, 104, 146, 363, 1243, 1417, 1582
PleI GAGTC 3 cut(s) 274, 601, 1542
PluTI GGCGCC 1 cut(s) 150
PmaCI CACGTG 1 cut(s) 1520
PmlI CACGTG 1 cut(s) 1520
PpsI GAGTC 3 cut(s) 274, 601, 1542
Ppu21I YACGTR 1 cut(s) 1520
PpuMI RGGWCCY 1 cut(s) 853
PshAI GACNNNNGTC 1 cut(s) 1209
Psp5II RGGWCCY 1 cut(s) 853
Psp6I CCWGG 3 cut(s) 655, 1222, 1540
PspCI CACGTG 1 cut(s) 1520
PspGI CCWGG 3 cut(s) 655, 1222, 1540
PspN4I GGNNCC 8 cut(s) 148, 303, 678, 972, 1085, 1167, 1197, 1377
PspPI GGNCC 6 cut(s) 334, 653, 659, 853, 1084, 1460
PspPPI RGGWCCY 1 cut(s) 853
PsuI RGATCY 3 cut(s) 292, 301, 1235
PsyI GACNNNGTC 2 cut(s) 657, 795
RsaI GTAC 5 cut(s) 62, 437, 1393, 1411, 1456
RsaNI GTAC 5 cut(s) 61, 436, 1392, 1410, 1455
SapI GCTCTTC 1 cut(s) 200
SaqAI TTAA 4 cut(s) 537, 990, 1218, 1530
SatI GCNGC 8 cut(s) 34, 94, 103, 145, 362, 1242, 1416, 1581
Sau3AI GATC 5 cut(s) 44, 292, 301, 1235, 1522
Sau96I GGNCC 6 cut(s) 334, 653, 659, 853, 1084, 1460
ScaI AGTACT 1 cut(s) 1393
SchI GAGTC 3 cut(s) 274, 601, 1542
ScrFI CCNGG 4 cut(s) 657, 1224, 1431, 1542
SduI GDGCHC 3 cut(s) 460, 897, 1327
SexAI ACCWGGT 1 cut(s) 655
SfaNI GCATC 6 cut(s) 383, 477, 1003, 1171, 1485, 1561
SfoI GGCGCC 1 cut(s) 148
SfuI TTCGAA 2 cut(s) 251, 960
SinI GGWCC 5 cut(s) 334, 653, 659, 853, 1460
SmlI CTYRAG 1 cut(s) 609
SmoI CTYRAG 1 cut(s) 609
Sse9I AATT 3 cut(s) 956, 987, 1479
SsiI CCGC 9 cut(s) 33, 36, 42, 102, 116, 144, 362, 516, 1160
SspDI GGCGCC 1 cut(s) 146
SspI AATATT 1 cut(s) 906
SspMI CTAG 5 cut(s) 569, 681, 690, 857, 1602
StyD4I CCNGG 4 cut(s) 655, 1222, 1429, 1540
StyI CCWWGG 2 cut(s) 111, 401
TaaI ACNGT 4 cut(s) 74, 462, 1322, 1396
TaiI ACGT 1 cut(s) 1522
TaqI TCGA 7 cut(s) 47, 169, 251, 960, 1292, 1424, 1436
TaqII GACCGA 1 cut(s) 1361
TasI AATT 3 cut(s) 956, 987, 1479
TatI WGTACW 2 cut(s) 435, 1391
TauI GCSGC 4 cut(s) 36, 105, 147, 364
TfiI GAWTC 2 cut(s) 166, 1094
Tru1I TTAA 4 cut(s) 537, 990, 1218, 1530
Tru9I TTAA 4 cut(s) 537, 990, 1218, 1530
TscAI CASTG 4 cut(s) 199, 877, 916, 1327
TseFI GTSAC 3 cut(s) 154, 229, 1503
TseI GCWGC 4 cut(s) 93, 1241, 1415, 1580
Tsp45I GTSAC 3 cut(s) 154, 229, 1503
TspGWI ACGGA 1 cut(s) 78
TspRI CASTG 4 cut(s) 199, 877, 916, 1327
Tth111I GACNNNGTC 2 cut(s) 657, 795
Van91I CCANNNNNTGG 1 cut(s) 1448
VneI GTGCAC 1 cut(s) 456
VpaK11BI GGWCC 5 cut(s) 334, 653, 659, 853, 1460
XagI CCTNNNNNAGG 1 cut(s) 1404
XapI RAATTY 1 cut(s) 956
XbaI TCTAGA 1 cut(s) 568
XspI CTAG 5 cut(s) 569, 681, 690, 857, 1602
ZrmI AGTACT 1 cut(s) 1393
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.