Rh6BG319600

Transcription initiation factor IIF subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0001724
Physical Location & Seq
Reverse (-)
55085850 .. 55093198
7349 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG319600.1

Sequence Viewer

Length: 996 bp
ATGGTGTTACATAGAAGAGAGGCTCACCTCAAAAAGTTGACGAGTGAACATGAGCTGTGGGTCTTATCAAGCCTTTTGTTGGATTCCTTTTTCATGATGGGCACAAATATTTCTCAACTGATGTGCAGGCTGGCAAATTTGAGTCTGGGCAGCTTGGGAAAACTGTATAGTGAGTATGATGTTGTGCGTGCAAACAATGATGACAAGAACTACTTCATCGGGAACATCTCAGATAACGATGATGAGGAACAAGAGGGAGCAAGGAAAAATAGACTTGAACCCAAGAGAAGATATGGTGATGATGAAGGAAACATCTCAGATAATAGTGATGAGGAAGAAGATGTAGCAAGGAAAAACATACTTGAACTCGAGAGAATAGGTGGCGATGATGAAGGTACAAGTGGAGATGATGACTTTGATAATGATGATAATGAGAAGGATGATGACTGGAAGCTTGAGGAGATTTTTAGCGATGATGATGAAAATAATACCAAGGAAATAGAAGACTTTGTAACCGAGGATGATGAGGAAGATGATAATAATGAAGAGGAGGGTGGGCTTAGTGAATCCGGAAAAGAGTTGAAGAAGCTACTTGGGCACCTCATTGATCATTCTAGATCGGCTCAGACTACCCCATCTCCCTCCAAGCCATCCAAAAGTATGAGAAAACGATATGATGATGATACCGAGGCATTTAGCTGTGCACCTCCCGAGAAGTTGAAGACTGAAAATGATAAAAAACCTTCCATAAAAGAAGAGTCTGCATCTGCTAGTCCTATCAGTGAGGAAGAAATCAGGGCTGTTCTTGAGAAAGAACTAGCGATTTCCTTACAAGACCTTGTTGACCACTATACAGGAAGAGTCGAATCGAATGAGGAGCTGAAGGCTTTAATAGCAGGGTTGAGGAGTATGTCTTACAAAATACTATACGGTAAACACAGACTTTCCAAATTTACTTCTAATCACGGATACGTCTTTTTTGGTGTATCAGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005515 GO:0008022
KEGG Pathways
Metabolic & Signaling
ko03022 map03022
Pfam Domains
Protein Families
TFIIF_alpha

Protein Analysis

331

Amino Acids

37.61

Weight (kDa)

4.51

Isoelectric Point (pI)

55.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TFIIF_alpha PF05793 206 - 304 2.2e-06 Transcription initiation factor IIF, alpha subunit (TFIIF-alpha)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 597
AccIII TCCGGA 1 cut(s) 569
AcsI RAATTY 2 cut(s) 136, 950
AcuI CTGAAG 1 cut(s) 902
AfaI GTAC 1 cut(s) 397
AfiI CCNNNNNNNGG 1 cut(s) 79
AgsI TTSAA 4 cut(s) 278, 365, 583, 721
AluBI AGCT 6 cut(s) 55, 153, 454, 589, 699, 880
AluI AGCT 6 cut(s) 55, 153, 454, 589, 699, 880
Alw21I GWGCWC 1 cut(s) 706
Alw44I GTGCAC 1 cut(s) 702
Ama87I CYCGRG 2 cut(s) 368, 710
Aor13HI TCCGGA 1 cut(s) 569
ApaLI GTGCAC 1 cut(s) 702
ApeKI GCWGC 1 cut(s) 150
ApoI RAATTY 2 cut(s) 136, 950
Asp700I GAANNNNTTC 1 cut(s) 212
AsuHPI GGTGA 2 cut(s) 17, 308
AvaI CYCGRG 2 cut(s) 368, 710
BaeGI GKGCMC 3 cut(s) 104, 600, 706
BanI GGYRCC 1 cut(s) 597
BbsI GAAGAC 2 cut(s) 510, 728
Bbv12I GWGCWC 1 cut(s) 706
BbvI GCAGC 1 cut(s) 162
BccI CCATC 3 cut(s) 91, 643, 658
BciVI GTATCC 1 cut(s) 962
BclI TGATCA 1 cut(s) 607
BfaI CTAG 3 cut(s) 615, 771, 818
BfuI GTATCC 1 cut(s) 962
BisI GCNGC 1 cut(s) 151
BlsI GCNGC 1 cut(s) 152
BmeT110I CYCGRG 2 cut(s) 368, 710
BmiI GGNNCC 1 cut(s) 599
BmsI GCATC 1 cut(s) 773
BpiI GAAGAC 2 cut(s) 510, 728
BpuEI CTTGAG 2 cut(s) 476, 827
BsaJI CCNNGG 3 cut(s) 492, 516, 687
BsaWI WCCGGW 1 cut(s) 569
BsaXI ACNNNNNCTCC 2 cut(s) 622, 652
Bsc4I CCNNNNNNNGG 1 cut(s) 79
Bse1I ACTGG 1 cut(s) 452
BseAI TCCGGA 1 cut(s) 569
BseDI CCNNGG 3 cut(s) 492, 516, 687
BseGI GGATG 3 cut(s) 445, 526, 650
BseLI CCNNNNNNNGG 1 cut(s) 79
BseMII CTCAG 3 cut(s) 243, 330, 638
BseNI ACTGG 1 cut(s) 452
BseRI GAGGAG 4 cut(s) 473, 563, 890, 919
BseSI GKGCMC 3 cut(s) 104, 600, 706
BseXI GCAGC 1 cut(s) 162
BsgI GTGCAG 1 cut(s) 145
BshNI GGYRCC 1 cut(s) 597
BsiHKAI GWGCWC 1 cut(s) 706
BsiHKCI CYCGRG 2 cut(s) 368, 710
BsiSI CCGG 1 cut(s) 570
BslI CCNNNNNNNGG 1 cut(s) 79
BsoBI CYCGRG 2 cut(s) 368, 710
Bsp1286I GDGCHC 3 cut(s) 104, 600, 706
Bsp13I TCCGGA 1 cut(s) 569
Bsp143I GATC 2 cut(s) 607, 617
BspCNI CTCAG 3 cut(s) 242, 329, 637
BspEI TCCGGA 1 cut(s) 569
BspHI TCATGA 1 cut(s) 93
BspLI GGNNCC 1 cut(s) 599
BspT107I GGYRCC 1 cut(s) 597
BsrI ACTGG 1 cut(s) 452
BssECI CCNNGG 3 cut(s) 492, 516, 687
BssMI GATC 2 cut(s) 607, 617
BssT1I CCWWGG 1 cut(s) 492
Bst4CI ACNGT 2 cut(s) 165, 932
Bst6I CTCTTC 4 cut(s) 10, 540, 750, 853
BstC8I GCNNGC 3 cut(s) 128, 132, 189
BstDEI CTNAG 4 cut(s) 229, 316, 560, 624
BstF5I GGATG 3 cut(s) 445, 526, 650
BstKTI GATC 2 cut(s) 610, 620
BstMBI GATC 2 cut(s) 607, 617
BstMWI GCNNNNNNNGC 2 cut(s) 595, 893
BstSLI GKGCMC 3 cut(s) 104, 600, 706
BstV1I GCAGC 1 cut(s) 162
BstV2I GAAGAC 2 cut(s) 510, 728
BsuI GTATCC 1 cut(s) 962
BtgZI GCGATG 2 cut(s) 399, 486
BtsCI GGATG 3 cut(s) 445, 526, 650
BtsIMutI CAGTG 2 cut(s) 787, 996
Cac8I GCNNGC 3 cut(s) 128, 132, 189
CciI TCATGA 1 cut(s) 93
Csp6I GTAC 1 cut(s) 396
CviAII CATG 2 cut(s) 50, 94
CviQI GTAC 1 cut(s) 396
DdeI CTNAG 4 cut(s) 229, 316, 560, 624
DpnI GATC 2 cut(s) 609, 619
DpnII GATC 2 cut(s) 607, 617
Eam1104I CTCTTC 4 cut(s) 10, 540, 750, 853
EarI CTCTTC 4 cut(s) 10, 540, 750, 853
Eco130I CCWWGG 1 cut(s) 492
Eco57I CTGAAG 1 cut(s) 902
Eco88I CYCGRG 2 cut(s) 368, 710
EcoT14I CCWWGG 1 cut(s) 492
ErhI CCWWGG 1 cut(s) 492
FaeI CATG 2 cut(s) 53, 97
FalI AAGNNNNNCTT 2 cut(s) 197, 229
FatI CATG 2 cut(s) 49, 93
FbaI TGATCA 1 cut(s) 607
Fnu4HI GCNGC 1 cut(s) 151
FokI GGATG 3 cut(s) 452, 533, 637
Fsp4HI GCNGC 1 cut(s) 151
FspBI CTAG 3 cut(s) 615, 771, 818
GluI GCNGC 1 cut(s) 151
HapII CCGG 1 cut(s) 570
Hin1II CATG 2 cut(s) 53, 97
HincII GTYRAC 2 cut(s) 39, 844
HindII GTYRAC 2 cut(s) 39, 844
HindIII AAGCTT 1 cut(s) 452
HinfI GANTC 6 cut(s) 83, 142, 566, 758, 861, 866
HpaII CCGG 1 cut(s) 570
HphI GGTGA 2 cut(s) 17, 308
Hpy166II GTNNAC 5 cut(s) 39, 47, 704, 844, 935
Hpy188I TCNGA 3 cut(s) 232, 319, 627
Hpy188III TCNNGA 7 cut(s) 94, 220, 370, 570, 615, 710, 806
Hpy8I GTNNAC 5 cut(s) 39, 47, 704, 844, 935
HpyAV CCTTC 5 cut(s) 299, 386, 430, 753, 877
HpyCH4III ACNGT 2 cut(s) 165, 932
HpyCH4IV ACGT 1 cut(s) 972
HpyCH4V TGCA 4 cut(s) 126, 191, 704, 764
HpyF10VI GCNNNNNNNGC 2 cut(s) 595, 893
HpyF3I CTNAG 4 cut(s) 229, 316, 560, 624
HpySE526I ACGT 1 cut(s) 972
Hsp92II CATG 2 cut(s) 53, 97
Kpn2I TCCGGA 1 cut(s) 569
Ksp22I TGATCA 1 cut(s) 607
Kzo9I GATC 2 cut(s) 607, 617
LmnI GCTCC 2 cut(s) 257, 877
LpnPI CCDG 8 cut(s) 112, 116, 131, 433, 583, 781, 840, 882
Lsp1109I GCAGC 1 cut(s) 162
LweI GCATC 1 cut(s) 773
MaeI CTAG 3 cut(s) 615, 771, 818
MaeII ACGT 1 cut(s) 972
MaeIII GTNAC 2 cut(s) 6, 511
MalI GATC 2 cut(s) 609, 619
MboI GATC 2 cut(s) 607, 617
MhlI GDGCHC 3 cut(s) 104, 600, 706
MluCI AATT 2 cut(s) 136, 950
MlyI GAGTC 3 cut(s) 151, 767, 870
MmeI TCCRAC 1 cut(s) 60
MroI TCCGGA 1 cut(s) 569
MroXI GAANNNNTTC 1 cut(s) 212
MseI TTAA 1 cut(s) 890
MspI CCGG 1 cut(s) 570
MwoI GCNNNNNNNGC 2 cut(s) 595, 893
NdeII GATC 2 cut(s) 607, 617
NlaIII CATG 2 cut(s) 53, 97
NlaIV GGNNCC 1 cut(s) 599
PaeR7I CTCGAG 1 cut(s) 368
PagI TCATGA 1 cut(s) 93
PdmI GAANNNNTTC 1 cut(s) 212
PfeI GAWTC 3 cut(s) 83, 566, 866
PkrI GCNGC 1 cut(s) 152
PleI GAGTC 3 cut(s) 150, 766, 869
PpsI GAGTC 3 cut(s) 150, 766, 869
PspN4I GGNNCC 1 cut(s) 599
RsaI GTAC 1 cut(s) 397
RsaNI GTAC 1 cut(s) 396
SaqAI TTAA 1 cut(s) 890
SatI GCNGC 1 cut(s) 151
Sau3AI GATC 2 cut(s) 607, 617
SchI GAGTC 3 cut(s) 151, 767, 870
SduI GDGCHC 3 cut(s) 104, 600, 706
SfaNI GCATC 1 cut(s) 773
Sfr274I CTCGAG 1 cut(s) 368
SlaI CTCGAG 1 cut(s) 368
SmlI CTYRAG 3 cut(s) 368, 455, 806
SmoI CTYRAG 3 cut(s) 368, 455, 806
Sse9I AATT 2 cut(s) 136, 950
SspI AATATT 1 cut(s) 109
SspMI CTAG 3 cut(s) 615, 771, 818
StyI CCWWGG 1 cut(s) 492
TaaI ACNGT 2 cut(s) 165, 932
TaiI ACGT 1 cut(s) 975
TaqI TCGA 3 cut(s) 369, 864, 869
TasI AATT 2 cut(s) 136, 950
TfiI GAWTC 3 cut(s) 83, 566, 866
Tru1I TTAA 1 cut(s) 890
Tru9I TTAA 1 cut(s) 890
TscAI CASTG 2 cut(s) 787, 996
TseI GCWGC 1 cut(s) 150
TspDTI ATGAA 6 cut(s) 82, 205, 318, 405, 495, 558
TspGWI ACGGA 1 cut(s) 981
TspRI CASTG 2 cut(s) 787, 996
VneI GTGCAC 1 cut(s) 702
XapI RAATTY 2 cut(s) 136, 950
XbaI TCTAGA 1 cut(s) 614
XhoI CTCGAG 1 cut(s) 368
XmnI GAANNNNTTC 1 cut(s) 212
XspI CTAG 3 cut(s) 615, 771, 818
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.