Rw7G019510

Transcription initiation factor IIF subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0001724
Physical Location & Seq
Forward (+)
21650392 .. 21651893
1502 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G019510.1

Sequence Viewer

Length: 654 bp
ATGGGCAAGGCGTTTTTGGATGATGACGATGATGATAATAATGAAGAGGAGGGTGGGCTCAGCAAATCAGGAAAAGAGTTGAAGAAGCAACTTGGGCGAAGTGGTGGCCTGAATGATTCAGATGCAGAGGACGACGACGACGACGACGATGACGATTTCTTACGATTTGTACAGATGGATGATGATATTGCCTTGCCTCAAGCGCTTGCACCAAAGCAGAAGGATGCACCCAAACAGGAACCAGTTACAAGTCCTATTAAACCCGGGACTTCTGGATCTGCTGGTAAAGCAAACCCATCTGCCTCCAAGTCGGCGAAGGGAAAGAGAAAATTAAATGGTGATGACGCTAAAGCATCCAACAGTGCACCTCCCAAGAAGGTGAAGACCGAAAATTGTGCCTGCAAAAGGTACTGCTGCATCCTTGAAACGGGTTCAACACCATCTGGTGGTCCTACAGGTCCTGTATCTGAGGAAGAAATTAGGGCAGTTTTGATGCAAAAGTCACCCATCACCACACGCGATCTTGTTAATAACTTCCCCCGGATGACTCAAATCCAAAGAGGTATGGAGAAGGATGCTTTTGCAGGAATCCTGAGGAAAATGTTTAGGATACAGAAAAATGGGATCAGCTATGTTGTATTGAGGGAGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005515 GO:0008022
KEGG Pathways
Metabolic & Signaling
ko03022 map03022
Pfam Domains
Protein Families
TFIIF_alpha

Protein Analysis

217

Amino Acids

23.51

Weight (kDa)

5.29

Isoelectric Point (pI)

43.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TFIIF_alpha PF05793 7 - 215 3.2e-36 Transcription initiation factor IIF, alpha subunit (TFIIF-alpha)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 446
AccII CGCG 1 cut(s) 519
AclWI GGATC 2 cut(s) 283, 632
AfaI GTAC 2 cut(s) 171, 410
AfeI AGCGCT 1 cut(s) 204
AfiI CCNNNNNNNGG 3 cut(s) 405, 427, 446
AgsI TTSAA 3 cut(s) 82, 425, 435
AluBI AGCT 1 cut(s) 630
AluI AGCT 1 cut(s) 630
Alw21I GWGCWC 1 cut(s) 367
Alw44I GTGCAC 1 cut(s) 363
AlwI GGATC 2 cut(s) 283, 632
AlwNI CAGNNNCTG 1 cut(s) 461
Ama87I CYCGRG 1 cut(s) 263
Aor51HI AGCGCT 1 cut(s) 204
AoxI GGCC 1 cut(s) 106
ApaLI GTGCAC 1 cut(s) 363
ApeKI GCWGC 1 cut(s) 414
AspLEI GCGC 1 cut(s) 205
AspS9I GGNCC 2 cut(s) 449, 458
AsuC2I CCSGG 3 cut(s) 264, 265, 541
AsuHPI GGTGA 4 cut(s) 350, 391, 495, 502
AvaI CYCGRG 1 cut(s) 263
AvaII GGWCC 2 cut(s) 449, 458
AxyI CCTNAGG 1 cut(s) 593
BaeGI GKGCMC 1 cut(s) 367
BanII GRGCYC 1 cut(s) 60
BbsI GAAGAC 1 cut(s) 389
Bbv12I GWGCWC 1 cut(s) 367
BbvI GCAGC 1 cut(s) 401
BccI CCATC 4 cut(s) 169, 304, 448, 515
BcgI CGANNNNNNTGC 2 cut(s) 377, 411
BciVI GTATCC 1 cut(s) 603
BcnI CCSGG 3 cut(s) 264, 265, 541
BfmI CTRYAG 1 cut(s) 453
BfoI RGCGCY 1 cut(s) 206
BfuI GTATCC 1 cut(s) 603
BisI GCNGC 1 cut(s) 415
BlpI GCTNAGC 1 cut(s) 59
BlsI GCNGC 1 cut(s) 416
Bme1390I CCNGG 3 cut(s) 264, 265, 541
Bme18I GGWCC 2 cut(s) 449, 458
BmeT110I CYCGRG 1 cut(s) 263
BmgT120I GGNCC 2 cut(s) 449, 458
BmiI GGNNCC 1 cut(s) 240
BmrFI CCNGG 3 cut(s) 264, 265, 541
BmsI GCATC 6 cut(s) 112, 214, 362, 426, 483, 565
BpiI GAAGAC 1 cut(s) 389
Bpu1102I GCTNAGC 1 cut(s) 59
BpuEI CTTGAG 1 cut(s) 183
BpuMI CCSGG 3 cut(s) 264, 265, 541
BsaJI CCNNGG 2 cut(s) 263, 539
Bsc4I CCNNNNNNNGG 3 cut(s) 405, 427, 446
Bse1I ACTGG 1 cut(s) 242
Bse21I CCTNAGG 1 cut(s) 593
BseDI CCNNGG 2 cut(s) 263, 539
BseGI GGATG 7 cut(s) 25, 184, 229, 353, 417, 549, 580
BseLI CCNNNNNNNGG 3 cut(s) 405, 427, 446
BseMII CTCAG 3 cut(s) 73, 459, 584
BseNI ACTGG 1 cut(s) 242
BseRI GAGGAG 1 cut(s) 62
BseSI GKGCMC 1 cut(s) 367
BseXI GCAGC 1 cut(s) 401
Bsh1236I CGCG 1 cut(s) 519
BshFI GGCC 1 cut(s) 108
BsiHKAI GWGCWC 1 cut(s) 367
BsiHKCI CYCGRG 1 cut(s) 263
BsiSI CCGG 2 cut(s) 264, 541
BslFI GGGAC 1 cut(s) 280
BslI CCNNNNNNNGG 3 cut(s) 405, 427, 446
BsmFI GGGAC 1 cut(s) 280
BsnI GGCC 1 cut(s) 108
BsoBI CYCGRG 1 cut(s) 263
Bsp1286I GDGCHC 2 cut(s) 60, 367
Bsp1407I TGTACA 1 cut(s) 169
Bsp143I GATC 3 cut(s) 275, 520, 624
Bsp1720I GCTNAGC 1 cut(s) 59
BspANI GGCC 1 cut(s) 108
BspCNI CTCAG 3 cut(s) 72, 460, 585
BspFNI CGCG 1 cut(s) 519
BspLI GGNNCC 1 cut(s) 240
BspPI GGATC 2 cut(s) 283, 632
BsrGI TGTACA 1 cut(s) 169
BsrI ACTGG 1 cut(s) 242
BssECI CCNNGG 2 cut(s) 263, 539
BssMI GATC 3 cut(s) 275, 520, 624
Bst4CI ACNGT 1 cut(s) 362
Bst6I CTCTTC 1 cut(s) 39
BstAUI TGTACA 1 cut(s) 169
BstC8I GCNNGC 2 cut(s) 207, 400
BstDEI CTNAG 3 cut(s) 59, 468, 593
BstENI CCTNNNNNAGG 1 cut(s) 403
BstF5I GGATG 7 cut(s) 25, 184, 229, 353, 417, 549, 580
BstFNI CGCG 1 cut(s) 519
BstH2I RGCGCY 1 cut(s) 206
BstHHI GCGC 1 cut(s) 205
BstKTI GATC 3 cut(s) 278, 523, 627
BstMBI GATC 3 cut(s) 275, 520, 624
BstMWI GCNNNNNNNGC 3 cut(s) 94, 202, 287
BstSCI CCNGG 3 cut(s) 262, 263, 539
BstSFI CTRYAG 1 cut(s) 453
BstSLI GKGCMC 1 cut(s) 367
BstUI CGCG 1 cut(s) 519
BstV1I GCAGC 1 cut(s) 401
BstV2I GAAGAC 1 cut(s) 389
BstX2I RGATCY 1 cut(s) 275
BstYI RGATCY 1 cut(s) 275
Bsu36I CCTNAGG 1 cut(s) 593
BsuI GTATCC 1 cut(s) 603
BsuRI GGCC 1 cut(s) 108
BtsCI GGATG 7 cut(s) 25, 184, 229, 353, 417, 549, 580
BtsIMutI CAGTG 1 cut(s) 367
Cac8I GCNNGC 2 cut(s) 207, 400
CaiI CAGNNNCTG 1 cut(s) 461
CfoI GCGC 1 cut(s) 205
Cfr13I GGNCC 2 cut(s) 449, 458
Cfr9I CCCGGG 1 cut(s) 263
CseI GACGC 1 cut(s) 353
Csp6I GTAC 2 cut(s) 170, 409
CviJI RGCY 3 cut(s) 58, 108, 630
CviKI_1 RGCY 3 cut(s) 58, 108, 630
CviQI GTAC 2 cut(s) 170, 409
DdeI CTNAG 3 cut(s) 59, 468, 593
DpnI GATC 3 cut(s) 277, 522, 626
DpnII GATC 3 cut(s) 275, 520, 624
Eam1104I CTCTTC 1 cut(s) 39
EarI CTCTTC 1 cut(s) 39
Eco24I GRGCYC 1 cut(s) 60
Eco47I GGWCC 2 cut(s) 449, 458
Eco47III AGCGCT 1 cut(s) 204
Eco81I CCTNAGG 1 cut(s) 593
Eco88I CYCGRG 1 cut(s) 263
EcoNI CCTNNNNNAGG 1 cut(s) 403
EcoO109I RGGNCCY 1 cut(s) 458
EcoT38I GRGCYC 1 cut(s) 60
FaiI YATR 2 cut(s) 566, 633
FaqI GGGAC 1 cut(s) 280
Fnu4HI GCNGC 1 cut(s) 415
FokI GGATG 7 cut(s) 32, 191, 236, 340, 404, 556, 587
FriOI GRGCYC 1 cut(s) 60
Fsp4HI GCNGC 1 cut(s) 415
GlaI GCGC 1 cut(s) 204
GluI GCNGC 1 cut(s) 415
HaeII RGCGCY 1 cut(s) 206
HaeIII GGCC 1 cut(s) 108
HapII CCGG 2 cut(s) 264, 541
HgaI GACGC 1 cut(s) 353
HhaI GCGC 1 cut(s) 205
Hin6I GCGC 1 cut(s) 203
HinP1I GCGC 1 cut(s) 203
HinfI GANTC 3 cut(s) 116, 547, 588
HpaII CCGG 2 cut(s) 264, 541
HphI GGTGA 4 cut(s) 350, 391, 495, 502
Hpy166II GTNNAC 1 cut(s) 365
Hpy188I TCNGA 2 cut(s) 121, 469
Hpy188III TCNNGA 3 cut(s) 69, 273, 592
Hpy8I GTNNAC 1 cut(s) 365
Hpy99I CGWCG 5 cut(s) 137, 140, 143, 146, 149
HpyAV CCTTC 4 cut(s) 214, 310, 370, 565
HpyCH4III ACNGT 1 cut(s) 362
HpyCH4V TGCA 8 cut(s) 125, 209, 227, 365, 402, 417, 496, 584
HpyF10VI GCNNNNNNNGC 3 cut(s) 94, 202, 287
HpyF3I CTNAG 3 cut(s) 59, 468, 593
HspAI GCGC 1 cut(s) 203
Kzo9I GATC 3 cut(s) 275, 520, 624
Lsp1109I GCAGC 1 cut(s) 401
LweI GCATC 6 cut(s) 112, 214, 362, 426, 483, 565
MaeIII GTNAC 2 cut(s) 244, 501
MalI GATC 3 cut(s) 277, 522, 626
MboI GATC 3 cut(s) 275, 520, 624
MboII GAAGA 4 cut(s) 56, 94, 394, 485
MflI RGATCY 1 cut(s) 275
MhlI GDGCHC 2 cut(s) 60, 367
MluCI AATT 3 cut(s) 329, 391, 477
MlyI GAGTC 1 cut(s) 541
MmeI TCCRAC 1 cut(s) 381
MseI TTAA 3 cut(s) 258, 332, 528
MspI CCGG 2 cut(s) 264, 541
MspR9I CCNGG 3 cut(s) 264, 265, 541
MvnI CGCG 1 cut(s) 519
MwoI GCNNNNNNNGC 3 cut(s) 94, 202, 287
NciI CCSGG 3 cut(s) 264, 265, 541
NdeII GATC 3 cut(s) 275, 520, 624
NlaIV GGNNCC 1 cut(s) 240
NmuCI GTSAC 1 cut(s) 501
PcsI WCGNNNNNNNCGW 4 cut(s) 138, 141, 144, 150
PfeI GAWTC 2 cut(s) 116, 588
PflMI CCANNNNNTGG 1 cut(s) 446
PkrI GCNGC 1 cut(s) 416
PleI GAGTC 1 cut(s) 541
PpsI GAGTC 1 cut(s) 541
PpuMI RGGWCCY 1 cut(s) 458
Psp5II RGGWCCY 1 cut(s) 458
PspN4I GGNNCC 1 cut(s) 240
PspPI GGNCC 2 cut(s) 449, 458
PspPPI RGGWCCY 1 cut(s) 458
PstNI CAGNNNCTG 1 cut(s) 461
PsuI RGATCY 1 cut(s) 275
RsaI GTAC 2 cut(s) 171, 410
RsaNI GTAC 2 cut(s) 170, 409
SaqAI TTAA 3 cut(s) 258, 332, 528
SatI GCNGC 1 cut(s) 415
Sau3AI GATC 3 cut(s) 275, 520, 624
Sau96I GGNCC 2 cut(s) 449, 458
SchI GAGTC 1 cut(s) 541
ScrFI CCNGG 3 cut(s) 264, 265, 541
SduI GDGCHC 2 cut(s) 60, 367
SetI ASST 6 cut(s) 370, 381, 410, 460, 565, 632
SfaNI GCATC 6 cut(s) 112, 214, 362, 426, 483, 565
SfcI CTRYAG 1 cut(s) 453
SinI GGWCC 2 cut(s) 449, 458
SmaI CCCGGG 1 cut(s) 265
SmlI CTYRAG 1 cut(s) 198
SmoI CTYRAG 1 cut(s) 198
Sse9I AATT 3 cut(s) 329, 391, 477
StyD4I CCNGG 3 cut(s) 262, 263, 539
TaaI ACNGT 1 cut(s) 362
TaqII GACCGA 1 cut(s) 401
TasI AATT 3 cut(s) 329, 391, 477
TatI WGTACW 1 cut(s) 169
TfiI GAWTC 2 cut(s) 116, 588
Tru1I TTAA 3 cut(s) 258, 332, 528
Tru9I TTAA 3 cut(s) 258, 332, 528
TscAI CASTG 1 cut(s) 367
TseFI GTSAC 1 cut(s) 501
TseI GCWGC 1 cut(s) 414
Tsp45I GTSAC 1 cut(s) 501
TspDTI ATGAA 1 cut(s) 57
TspMI CCCGGG 1 cut(s) 263
TspRI CASTG 1 cut(s) 367
Van91I CCANNNNNTGG 1 cut(s) 446
VneI GTGCAC 1 cut(s) 363
VpaK11BI GGWCC 2 cut(s) 449, 458
XagI CCTNNNNNAGG 1 cut(s) 403
XmaI CCCGGG 1 cut(s) 263
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.