Rh5CG313700

Transcription initiation factor IIF subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0001724
Physical Location & Seq
Reverse (-)
34619767 .. 34621212
1446 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG313700.1

Sequence Viewer

Length: 540 bp
ATGGAATTGACAGAACAAAACACCAAAGAAAGTGGAGAGGCATTTAGTTATGATGACGAAGATTATAACAATGATGAAGATGGCAGACTGAGCAAATCTGGAAACGAGTTAAAGAAGCTACTTGGGCGCCTGAATAAGTCAGATACAGAGGATGACGATGACAATGACAATGCCGATGGGGATGATGATATCGATGATGTTATTGACAAGGATCCCCTAAAAGCAGTGCCTTCTGGAGCTTCTCAAATAACCCCATCTACCTCCCAAGGAAAGAGAAAAAGTAACGATGATGAAGCCAAGGCATATACCGGTGCACCTCACAAGAAGGCGAAGACTGAAAATGGTCAAAAACCTTCTGTCAAAGAAGAGCCTGCATCTGATAGTCTTGTCACTGAAGAAATCAAGGCTGTTTTGATGCAACAGTCACCAATCACCGTGTGTGATCTTCTTAATAACTTTCCAGGAAGACTCCGGCCAAAAGAGGAAATCAGATCCTCAGCTCCTATGCAAAGAAAGCTAACCTGGAGGGATCCACTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005515 GO:0008022
KEGG Pathways
Metabolic & Signaling
ko03022 map03022
Pfam Domains
Protein Families
TFIIF_alpha

Protein Analysis

179

Amino Acids

19.84

Weight (kDa)

4.54

Isoelectric Point (pI)

45.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TFIIF_alpha PF05793 15 - 165 1.1e-09 Transcription initiation factor IIF, alpha subunit (TFIIF-alpha)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 66
AccB1I GGYRCC 1 cut(s) 126
AclWI GGATC 5 cut(s) 206, 219, 486, 524, 537
AcoI YGGCCR 1 cut(s) 473
AcuI CTGAAG 1 cut(s) 414
AcyI GRCGYC 1 cut(s) 127
AdeI CACNNNGTG 1 cut(s) 438
AgeI ACCGGT 1 cut(s) 308
AjnI CCWGG 2 cut(s) 460, 521
AluBI AGCT 4 cut(s) 118, 239, 500, 517
AluI AGCT 4 cut(s) 118, 239, 500, 517
Alw21I GWGCWC 1 cut(s) 316
Alw44I GTGCAC 1 cut(s) 312
AlwI GGATC 5 cut(s) 206, 219, 486, 524, 537
AoxI GGCC 1 cut(s) 473
ApaLI GTGCAC 1 cut(s) 312
AsiGI ACCGGT 1 cut(s) 308
AspLEI GCGC 1 cut(s) 129
AsuHPI GGTGA 2 cut(s) 417, 424
BaeGI GKGCMC 1 cut(s) 316
BamHI GGATCC 2 cut(s) 211, 529
BanI GGYRCC 1 cut(s) 126
BbsI GAAGAC 2 cut(s) 338, 472
Bbv12I GWGCWC 1 cut(s) 316
BbvCI CCTCAGC 1 cut(s) 496
BccI CCATC 3 cut(s) 74, 170, 262
BciT130I CCWGG 2 cut(s) 462, 523
BfoI RGCGCY 1 cut(s) 130
Bme1390I CCNGG 2 cut(s) 462, 523
BmiI GGNNCC 3 cut(s) 128, 213, 531
BmrFI CCNGG 2 cut(s) 462, 523
BmsI GCATC 2 cut(s) 383, 405
BpiI GAAGAC 2 cut(s) 338, 472
BpmI CTGGAG 1 cut(s) 255
Bpu10I CCTNAGC 1 cut(s) 496
Bsa29I ATCGAT 1 cut(s) 192
BsaHI GRCGYC 1 cut(s) 127
BsaJI CCNNGG 2 cut(s) 265, 297
BsaWI WCCGGW 1 cut(s) 308
Bse118I RCCGGY 1 cut(s) 308
BseBI CCWGG 2 cut(s) 462, 523
BseCI ATCGAT 1 cut(s) 192
BseDI CCNNGG 2 cut(s) 265, 297
BseGI GGATG 2 cut(s) 157, 187
BseMII CTCAG 2 cut(s) 80, 510
BseSI GKGCMC 1 cut(s) 316
BshFI GGCC 1 cut(s) 475
BshNI GGYRCC 1 cut(s) 126
BshTI ACCGGT 1 cut(s) 308
BshVI ATCGAT 1 cut(s) 192
BsiHKAI GWGCWC 1 cut(s) 316
BsiSI CCGG 2 cut(s) 309, 472
BsnI GGCC 1 cut(s) 475
Bsp1286I GDGCHC 1 cut(s) 316
Bsp143I GATC 4 cut(s) 211, 442, 491, 529
BspANI GGCC 1 cut(s) 475
BspCNI CTCAG 2 cut(s) 81, 509
BspDI ATCGAT 1 cut(s) 192
BspLI GGNNCC 3 cut(s) 128, 213, 531
BspPI GGATC 5 cut(s) 206, 219, 486, 524, 537
BspQI GCTCTTC 1 cut(s) 360
BspT107I GGYRCC 1 cut(s) 126
BsrFI RCCGGY 1 cut(s) 308
BssAI RCCGGY 1 cut(s) 308
BssECI CCNNGG 2 cut(s) 265, 297
BssMI GATC 4 cut(s) 211, 442, 491, 529
BssNI GRCGYC 1 cut(s) 127
BssT1I CCWWGG 2 cut(s) 265, 297
Bst2UI CCWGG 2 cut(s) 462, 523
Bst4CI ACNGT 2 cut(s) 423, 436
Bst6I CTCTTC 1 cut(s) 360
BstACI GRCGYC 1 cut(s) 127
BstC8I GCNNGC 1 cut(s) 372
BstDEI CTNAG 2 cut(s) 89, 496
BstF5I GGATG 2 cut(s) 157, 187
BstH2I RGCGCY 1 cut(s) 130
BstHHI GCGC 1 cut(s) 129
BstKTI GATC 4 cut(s) 214, 445, 494, 532
BstMBI GATC 4 cut(s) 211, 442, 491, 529
BstMWI GCNNNNNNNGC 3 cut(s) 90, 124, 514
BstNI CCWGG 2 cut(s) 462, 523
BstSCI CCNGG 2 cut(s) 460, 521
BstSLI GKGCMC 1 cut(s) 316
BstV2I GAAGAC 2 cut(s) 338, 472
BstX2I RGATCY 3 cut(s) 211, 491, 529
BstYI RGATCY 3 cut(s) 211, 491, 529
Bsu15I ATCGAT 1 cut(s) 192
BsuRI GGCC 1 cut(s) 475
BsuTUI ATCGAT 1 cut(s) 192
BtsCI GGATG 2 cut(s) 157, 187
BtsI GCAGTG 1 cut(s) 231
BtsIMutI CAGTG 2 cut(s) 231, 390
Cac8I GCNNGC 1 cut(s) 372
CfoI GCGC 1 cut(s) 129
Cfr10I RCCGGY 1 cut(s) 308
ClaI ATCGAT 1 cut(s) 192
CspAI ACCGGT 1 cut(s) 308
CspCI CAANNNNNGTGG 2 cut(s) 13, 48
CviJI RGCY 8 cut(s) 118, 239, 296, 370, 407, 475, 500, 517
CviKI_1 RGCY 8 cut(s) 118, 239, 296, 370, 407, 475, 500, 517
DdeI CTNAG 2 cut(s) 89, 496
DinI GGCGCC 1 cut(s) 128
DpnI GATC 4 cut(s) 213, 444, 493, 531
DpnII GATC 4 cut(s) 211, 442, 491, 529
DraIII CACNNNGTG 1 cut(s) 438
EaeI YGGCCR 1 cut(s) 473
Eam1104I CTCTTC 1 cut(s) 360
EarI CTCTTC 1 cut(s) 360
Eco130I CCWWGG 2 cut(s) 265, 297
Eco32I GATATC 1 cut(s) 190
Eco57I CTGAAG 1 cut(s) 414
EcoRII CCWGG 2 cut(s) 460, 521
EcoRV GATATC 1 cut(s) 190
EcoT14I CCWWGG 2 cut(s) 265, 297
EgeI GGCGCC 1 cut(s) 128
EheI GGCGCC 1 cut(s) 128
ErhI CCWWGG 2 cut(s) 265, 297
FaiI YATR 5 cut(s) 51, 66, 304, 306, 506
FokI GGATG 2 cut(s) 164, 194
GlaI GCGC 1 cut(s) 128
GsuI CTGGAG 1 cut(s) 255
HaeII RGCGCY 1 cut(s) 130
HaeIII GGCC 1 cut(s) 475
HapII CCGG 2 cut(s) 309, 472
HhaI GCGC 1 cut(s) 129
Hin1I GRCGYC 1 cut(s) 127
Hin6I GCGC 1 cut(s) 127
HinP1I GCGC 1 cut(s) 127
HinfI GANTC 1 cut(s) 468
HpaII CCGG 2 cut(s) 309, 472
HphI GGTGA 2 cut(s) 417, 424
Hpy166II GTNNAC 1 cut(s) 314
Hpy188I TCNGA 3 cut(s) 142, 379, 491
Hpy188III TCNNGA 2 cut(s) 99, 234
Hpy8I GTNNAC 1 cut(s) 314
HpyAV CCTTC 3 cut(s) 240, 319, 363
HpyCH4III ACNGT 2 cut(s) 423, 436
HpyCH4V TGCA 4 cut(s) 314, 374, 418, 508
HpyF10VI GCNNNNNNNGC 3 cut(s) 90, 124, 514
HpyF3I CTNAG 2 cut(s) 89, 496
Hsp92I GRCGYC 1 cut(s) 127
HspAI GCGC 1 cut(s) 127
KasI GGCGCC 1 cut(s) 126
Kzo9I GATC 4 cut(s) 211, 442, 491, 529
LguI GCTCTTC 1 cut(s) 360
LmnI GCTCC 2 cut(s) 236, 505
LweI GCATC 2 cut(s) 383, 405
MaeIII GTNAC 3 cut(s) 281, 388, 423
MalI GATC 4 cut(s) 213, 444, 493, 531
MboI GATC 4 cut(s) 211, 442, 491, 529
MboII GAAGA 7 cut(s) 71, 89, 343, 377, 407, 437, 477
MflI RGATCY 3 cut(s) 211, 491, 529
MhlI GDGCHC 1 cut(s) 316
MluCI AATT 1 cut(s) 5
Mly113I GGCGCC 1 cut(s) 127
MlyI GAGTC 1 cut(s) 462
MnlI CCTC 7 cut(s) 31, 142, 271, 327, 475, 505, 519
MseI TTAA 2 cut(s) 110, 450
MspI CCGG 2 cut(s) 309, 472
MspR9I CCNGG 2 cut(s) 462, 523
MvaI CCWGG 2 cut(s) 462, 523
MwoI GCNNNNNNNGC 3 cut(s) 90, 124, 514
NarI GGCGCC 1 cut(s) 127
NdeII GATC 4 cut(s) 211, 442, 491, 529
NlaIV GGNNCC 3 cut(s) 128, 213, 531
NmuCI GTSAC 2 cut(s) 388, 423
PciSI GCTCTTC 1 cut(s) 360
PfoI TCCNGGA 1 cut(s) 460
PinAI ACCGGT 1 cut(s) 308
PleI GAGTC 1 cut(s) 462
PluTI GGCGCC 1 cut(s) 130
PpsI GAGTC 1 cut(s) 462
PsiI TTATAA 1 cut(s) 66
Psp6I CCWGG 2 cut(s) 460, 521
PspGI CCWGG 2 cut(s) 460, 521
PspN4I GGNNCC 3 cut(s) 128, 213, 531
PsuI RGATCY 3 cut(s) 211, 491, 529
SapI GCTCTTC 1 cut(s) 360
SaqAI TTAA 2 cut(s) 110, 450
Sau3AI GATC 4 cut(s) 211, 442, 491, 529
SchI GAGTC 1 cut(s) 462
ScrFI CCNGG 2 cut(s) 462, 523
SduI GDGCHC 1 cut(s) 316
SetI ASST 8 cut(s) 120, 241, 263, 319, 355, 502, 519, 524
SfaNI GCATC 2 cut(s) 383, 405
SfoI GGCGCC 1 cut(s) 128
Sse9I AATT 1 cut(s) 5
SspDI GGCGCC 1 cut(s) 126
StyD4I CCNGG 2 cut(s) 460, 521
StyI CCWWGG 2 cut(s) 265, 297
TaaI ACNGT 2 cut(s) 423, 436
TaqI TCGA 1 cut(s) 192
TasI AATT 1 cut(s) 5
Tru1I TTAA 2 cut(s) 110, 450
Tru9I TTAA 2 cut(s) 110, 450
TscAI CASTG 2 cut(s) 231, 397
TseFI GTSAC 2 cut(s) 388, 423
Tsp45I GTSAC 2 cut(s) 388, 423
TspDTI ATGAA 2 cut(s) 90, 306
TspRI CASTG 2 cut(s) 231, 397
VneI GTGCAC 1 cut(s) 312
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.