RLG00000012344

Transcription initiation factor IIF subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0001724
Physical Location & Seq
Reverse (-)
17691433 .. 17692662
1230 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000012344

Sequence Viewer

Length: 510 bp
ATGCAAGATGATGACGAAGATTATAACAATGATAAAGATGGCAGACTGAGCAAATCCGGAAACGAGTTAAAGAAGCTATTTGGGCGCCTGAATAAGTCAGATGCAGAGGATGACGATGACTCTGACAATGACAATGCCGATGGGGATGATGATATGGATGATGTTATTGACAAGGGTTCCCTAAAAGCAGTGCCTTCTGGAACTTCTCAAACAACCCCTAGGGAAAGAGAAAACGGTCAAAAACCTTCTGTCAAAGAAGAGCCTGCATCTGCTAGTCCTGTCACTGAAGAAGAAATCAGGGCTGTTTTGATGCAACAGTCTCCAATCACCGTGCGTGATCTTCTTAATAACTTTCCAGGAAGACTCCGGTCAAAAGAGGAGAAGGAGGTTTTTACAGCAATCCTGAAGAGAATGCACTCTCAGAAAACAATTTCCAGAAAACCAAAAGACAATGGAACCGGTTATTTTGTTGTACTGAGAGAAAGTGAATTGAATATGGCCTTCCCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005515 GO:0008022
KEGG Pathways
Metabolic & Signaling
ko03022 map03022
Pfam Domains
Protein Families
TFIIF_alpha

Protein Analysis

170

Amino Acids

18.95

Weight (kDa)

4.66

Isoelectric Point (pI)

49.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TFIIF_alpha PF05793 81 - 144 5.1e-12 Transcription initiation factor IIF, alpha subunit (TFIIF-alpha)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 24
AccB1I GGYRCC 1 cut(s) 84
AccIII TCCGGA 1 cut(s) 56
AcuI CTGAAG 2 cut(s) 306, 425
AcyI GRCGYC 1 cut(s) 85
AfaI GTAC 1 cut(s) 474
AgeI ACCGGT 1 cut(s) 458
AgsI TTSAA 1 cut(s) 493
AjnI CCWGG 1 cut(s) 355
AluBI AGCT 1 cut(s) 76
AluI AGCT 1 cut(s) 76
Alw26I GTCTC 1 cut(s) 324
Aor13HI TCCGGA 1 cut(s) 56
AoxI GGCC 1 cut(s) 498
AsiGI ACCGGT 1 cut(s) 458
AspA2I CCTAGG 1 cut(s) 218
AspLEI GCGC 1 cut(s) 87
AsuHPI GGTGA 1 cut(s) 319
AvrII CCTAGG 1 cut(s) 218
BanI GGYRCC 1 cut(s) 84
BbsI GAAGAC 1 cut(s) 367
BccI CCATC 2 cut(s) 32, 134
BciT130I CCWGG 1 cut(s) 357
BcoDI GTCTC 1 cut(s) 324
BfaI CTAG 2 cut(s) 219, 273
BfoI RGCGCY 1 cut(s) 88
BlnI CCTAGG 1 cut(s) 218
Bme1390I CCNGG 1 cut(s) 357
BmiI GGNNCC 3 cut(s) 86, 178, 457
BmrFI CCNGG 1 cut(s) 357
BmsI GCATC 3 cut(s) 91, 275, 300
BoxI GACNNNNGTC 1 cut(s) 367
BpiI GAAGAC 1 cut(s) 367
BsaHI GRCGYC 1 cut(s) 85
BsaJI CCNNGG 1 cut(s) 218
BsaWI WCCGGW 3 cut(s) 56, 366, 458
Bse118I RCCGGY 1 cut(s) 458
BseAI TCCGGA 1 cut(s) 56
BseBI CCWGG 1 cut(s) 357
BseDI CCNNGG 1 cut(s) 218
BseGI GGATG 3 cut(s) 115, 151, 163
BseMII CTCAG 3 cut(s) 38, 434, 467
BseRI GAGGAG 1 cut(s) 392
BshFI GGCC 1 cut(s) 500
BshNI GGYRCC 1 cut(s) 84
BshTI ACCGGT 1 cut(s) 458
BsiSI CCGG 3 cut(s) 57, 367, 459
BsmAI GTCTC 1 cut(s) 324
BsmI GAATGC 1 cut(s) 417
BsnI GGCC 1 cut(s) 500
Bsp13I TCCGGA 1 cut(s) 56
Bsp143I GATC 1 cut(s) 337
BspANI GGCC 1 cut(s) 500
BspCNI CTCAG 3 cut(s) 39, 433, 468
BspEI TCCGGA 1 cut(s) 56
BspLI GGNNCC 3 cut(s) 86, 178, 457
BspQI GCTCTTC 1 cut(s) 252
BspT107I GGYRCC 1 cut(s) 84
BsrFI RCCGGY 1 cut(s) 458
BssAI RCCGGY 1 cut(s) 458
BssECI CCNNGG 1 cut(s) 218
BssMI GATC 1 cut(s) 337
BssNI GRCGYC 1 cut(s) 85
BssT1I CCWWGG 1 cut(s) 218
Bst2UI CCWGG 1 cut(s) 357
Bst4CI ACNGT 3 cut(s) 236, 318, 331
Bst6I CTCTTC 2 cut(s) 252, 401
BstACI GRCGYC 1 cut(s) 85
BstC8I GCNNGC 1 cut(s) 264
BstDEI CTNAG 3 cut(s) 47, 420, 476
BstF5I GGATG 3 cut(s) 115, 151, 163
BstH2I RGCGCY 1 cut(s) 88
BstHHI GCGC 1 cut(s) 87
BstKTI GATC 1 cut(s) 340
BstMAI GTCTC 1 cut(s) 324
BstMBI GATC 1 cut(s) 337
BstMWI GCNNNNNNNGC 2 cut(s) 48, 82
BstNI CCWGG 1 cut(s) 357
BstPAI GACNNNNGTC 1 cut(s) 367
BstSCI CCNGG 1 cut(s) 355
BstV2I GAAGAC 1 cut(s) 367
BsuRI GGCC 1 cut(s) 500
BtsCI GGATG 3 cut(s) 115, 151, 163
BtsI GCAGTG 1 cut(s) 195
BtsIMutI CAGTG 2 cut(s) 195, 282
Cac8I GCNNGC 1 cut(s) 264
CfoI GCGC 1 cut(s) 87
Cfr10I RCCGGY 1 cut(s) 458
Csp6I GTAC 1 cut(s) 473
CspAI ACCGGT 1 cut(s) 458
CviJI RGCY 4 cut(s) 76, 262, 302, 500
CviKI_1 RGCY 4 cut(s) 76, 262, 302, 500
CviQI GTAC 1 cut(s) 473
DdeI CTNAG 3 cut(s) 47, 420, 476
DinI GGCGCC 1 cut(s) 86
DpnI GATC 1 cut(s) 339
DpnII GATC 1 cut(s) 337
Eam1104I CTCTTC 2 cut(s) 252, 401
EarI CTCTTC 2 cut(s) 252, 401
Eco130I CCWWGG 1 cut(s) 218
Eco57I CTGAAG 2 cut(s) 306, 425
EcoRII CCWGG 1 cut(s) 355
EcoT14I CCWWGG 1 cut(s) 218
EgeI GGCGCC 1 cut(s) 86
EheI GGCGCC 1 cut(s) 86
ErhI CCWWGG 1 cut(s) 218
FaiI YATR 4 cut(s) 24, 155, 497, 508
FokI GGATG 3 cut(s) 122, 158, 170
FspBI CTAG 2 cut(s) 219, 273
GlaI GCGC 1 cut(s) 86
HaeII RGCGCY 1 cut(s) 88
HaeIII GGCC 1 cut(s) 500
HapII CCGG 3 cut(s) 57, 367, 459
HhaI GCGC 1 cut(s) 87
Hin1I GRCGYC 1 cut(s) 85
Hin6I GCGC 1 cut(s) 85
HinP1I GCGC 1 cut(s) 85
HinfI GANTC 2 cut(s) 119, 363
HpaII CCGG 3 cut(s) 57, 367, 459
HphI GGTGA 1 cut(s) 319
Hpy188I TCNGA 3 cut(s) 100, 124, 423
Hpy188III TCNNGA 4 cut(s) 57, 198, 403, 435
HpyAV CCTTC 3 cut(s) 204, 255, 376
HpyCH4III ACNGT 3 cut(s) 236, 318, 331
HpyCH4V TGCA 5 cut(s) 4, 104, 266, 313, 415
HpyF10VI GCNNNNNNNGC 2 cut(s) 48, 82
HpyF3I CTNAG 3 cut(s) 47, 420, 476
Hsp92I GRCGYC 1 cut(s) 85
HspAI GCGC 1 cut(s) 85
KasI GGCGCC 1 cut(s) 84
Kpn2I TCCGGA 1 cut(s) 56
Kzo9I GATC 1 cut(s) 337
LguI GCTCTTC 1 cut(s) 252
LweI GCATC 3 cut(s) 91, 275, 300
MaeI CTAG 2 cut(s) 219, 273
MaeIII GTNAC 1 cut(s) 280
MalI GATC 1 cut(s) 339
MboI GATC 1 cut(s) 337
MboII GAAGA 7 cut(s) 29, 269, 299, 302, 332, 372, 418
MluCI AATT 2 cut(s) 429, 488
Mly113I GGCGCC 1 cut(s) 85
MlyI GAGTC 2 cut(s) 113, 357
MnlI CCTC 3 cut(s) 100, 370, 379
MroI TCCGGA 1 cut(s) 56
MseI TTAA 2 cut(s) 68, 345
MspI CCGG 3 cut(s) 57, 367, 459
MspR9I CCNGG 1 cut(s) 357
Mva1269I GAATGC 1 cut(s) 417
MvaI CCWGG 1 cut(s) 357
MwoI GCNNNNNNNGC 2 cut(s) 48, 82
NarI GGCGCC 1 cut(s) 85
NdeII GATC 1 cut(s) 337
NlaIV GGNNCC 3 cut(s) 86, 178, 457
NmuCI GTSAC 1 cut(s) 280
PciSI GCTCTTC 1 cut(s) 252
PctI GAATGC 1 cut(s) 417
PfoI TCCNGGA 1 cut(s) 355
PinAI ACCGGT 1 cut(s) 458
PleI GAGTC 2 cut(s) 113, 357
PluTI GGCGCC 1 cut(s) 88
PpsI GAGTC 2 cut(s) 113, 357
PshAI GACNNNNGTC 1 cut(s) 367
PsiI TTATAA 1 cut(s) 24
Psp6I CCWGG 1 cut(s) 355
PspGI CCWGG 1 cut(s) 355
PspN4I GGNNCC 3 cut(s) 86, 178, 457
RsaI GTAC 1 cut(s) 474
RsaNI GTAC 1 cut(s) 473
SapI GCTCTTC 1 cut(s) 252
SaqAI TTAA 2 cut(s) 68, 345
Sau3AI GATC 1 cut(s) 337
SchI GAGTC 2 cut(s) 113, 357
ScrFI CCNGG 1 cut(s) 357
SetI ASST 3 cut(s) 78, 247, 390
SfaNI GCATC 3 cut(s) 91, 275, 300
SfoI GGCGCC 1 cut(s) 86
Sse9I AATT 2 cut(s) 429, 488
SspDI GGCGCC 1 cut(s) 84
SspMI CTAG 2 cut(s) 219, 273
StyD4I CCNGG 1 cut(s) 355
StyI CCWWGG 1 cut(s) 218
TaaI ACNGT 3 cut(s) 236, 318, 331
TasI AATT 2 cut(s) 429, 488
TatI WGTACW 1 cut(s) 472
Tru1I TTAA 2 cut(s) 68, 345
Tru9I TTAA 2 cut(s) 68, 345
TscAI CASTG 2 cut(s) 195, 289
TseFI GTSAC 1 cut(s) 280
Tsp45I GTSAC 1 cut(s) 280
TspRI CASTG 2 cut(s) 195, 289
XmaJI CCTAGG 1 cut(s) 218
XspI CTAG 2 cut(s) 219, 273
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.