FvH4_2g24790

Tubby-like F-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0001645
Physical Location & Seq
Reverse (-)
20189376 .. 20193201
3826 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g24790.t2

Sequence Viewer

Length: 1215 bp
ATGTCTTTCAAGAGTATACTTCAGGATATGAAAGGTGAGTTTGGGAGCATTTCGAGGAAAGGGTTTGATGTGAAATTTGGGATGAAATCGAGGTCACACCGGGTGGTGCAGGACACCTCGTTCAGGATTGATGCTTTGAAGCAGAGTTGTTGGGCTAACATGCCGCCAGAGCTTTTGAGGGATGTGTTGATGAGAATTGAGGCGTCGGATGATACGTGGCCTCCGAGGAAGAATGTGGTTGCTTGTGCTGGTGTGTGCAAGAATTGGAGAGAGATCATGAAGGAGATTGTGAAGTCCCCGGAAGTTTCGGGGAAGCTGACGTTTCCAATCTCTTTGAAGCAGCCTGGTCCCAGGGACTCCCTTCTCCAGTGTTATATAAAGCGGAGTCGTAGCAACCAGACATATTATCTTTTCCTGGGCTTAAATCAAGCTTCAACTGATGATGGGAAGTTCCTTCTTGCTGCAAGAAAGTGTAGACGCCCAACATGCACAGATTACATCATCTCTCTGAATTCTGAAGATGTGTCAAAAGGCAACAGTGCCTTTGTTGGGAAGCTCAGATCAAATTTTCTGGGTACCAAATTCACAATCTATGATGCACAACCAACTAATACTGGAGCCAAAGTCACTAAATGTCATTCAACCAGACTTGTAAATATGAAACAAGTGTCACCCAGAGTTCCTGCTGGCAACTATCCTGTGGCGCACATTGCTTATGAGTTGAATGTCTTGGGTTCCAGAGGTCCAAGGAGAATGCAGTGTGTAATGGATAGCATCCCTGCTCGTGCTGTTGAGCCAGGAGGTGTAGCCCCTACGCAGACTGAATTTGTCGCCAGCAATTTGGATAGTATTCCATCACTCCCTTTCTTCAGATCAAAATCAGGCCGTGGAGAGAGTGTCCAATCTGGACTACAATCTGGTCAGAATGAAGGCATGCTGGTATTAAGGAACAAGGCACCTAGGTGGCACGAACAACTCCAGTGCTGGTGTTTGAACTTCAATGGACGAGTGACTGTTGCTTCAGTCAAGAATTTTCAGCTGGTTGCTTCTCCAGAGAACGGAGTTGCTGCTGGCCCAGAGCATGAGAATGTCATTCTTCAGTTTGGAAAAGTAGGCAAAGATGTATTCACAATGGATTACCAGTATCCAATCTCAGCCTTCCAGGCATTTGCTATCTGCCTTAGCAGCTTTGACACCAAGATTGCTTGTGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005543 GO:0008289 GO:0035091 GO:0043167 GO:0043168
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box Tub

Protein Analysis

405

Amino Acids

44.89

Weight (kDa)

9.46

Isoelectric Point (pI)

52.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tub PF01167 112 - 399 5.7e-108 Tub family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 575
AccB1I GGYRCC 2 cut(s) 575, 955
AccI GTMKAC 2 cut(s) 16, 475
AciI CCGC 2 cut(s) 164, 382
AcsI RAATTY 6 cut(s) 74, 511, 565, 581, 824, 1030
AcuI CTGAAG 5 cut(s) 5, 537, 853, 1005, 1082
AcyI GRCGYC 2 cut(s) 203, 478
AdeI CACNNNGTG 1 cut(s) 103
AfaI GTAC 1 cut(s) 577
AfiI CCNNNNNNNGG 3 cut(s) 123, 549, 1058
AgsI TTSAA 8 cut(s) 10, 139, 337, 435, 642, 724, 994, 1000
AjnI CCWGG 5 cut(s) 343, 350, 414, 796, 1161
AleI CACNNNNGTG 1 cut(s) 961
AloI GAACNNNNNNTCC 2 cut(s) 104, 136
AluBI AGCT 6 cut(s) 172, 316, 431, 556, 1039, 1188
AluI AGCT 6 cut(s) 172, 316, 431, 556, 1039, 1188
AoxI GGCC 3 cut(s) 218, 883, 1072
ApeKI GCWGC 4 cut(s) 340, 461, 1067, 1185
ApoI RAATTY 6 cut(s) 74, 511, 565, 581, 824, 1030
Asp718I GGTACC 1 cut(s) 575
AspA2I CCTAGG 1 cut(s) 959
AspLEI GCGC 1 cut(s) 706
AspS9I GGNCC 3 cut(s) 347, 743, 1073
AsuC2I CCSGG 2 cut(s) 101, 299
AsuHPI GGTGA 2 cut(s) 47, 663
AvaII GGWCC 2 cut(s) 347, 743
AvrII CCTAGG 1 cut(s) 959
BanI GGYRCC 2 cut(s) 575, 955
BauI CACGAG 1 cut(s) 783
BbvI GCAGC 4 cut(s) 352, 448, 1054, 1197
BccI CCATC 2 cut(s) 437, 862
BceAI ACGGC 1 cut(s) 870
BciT130I CCWGG 5 cut(s) 345, 352, 416, 798, 1163
BciVI GTATCC 1 cut(s) 1155
BcnI CCSGG 2 cut(s) 101, 299
BfaI CTAG 1 cut(s) 960
BfuI GTATCC 1 cut(s) 1155
BglI GCCNNNNNGGC 1 cut(s) 1163
BisI GCNGC 5 cut(s) 164, 341, 462, 1068, 1186
BlnI CCTAGG 1 cut(s) 959
BlsI GCNGC 5 cut(s) 165, 342, 463, 1069, 1187
Bme1390I CCNGG 7 cut(s) 101, 299, 345, 352, 416, 798, 1163
Bme18I GGWCC 2 cut(s) 347, 743
BmgT120I GGNCC 3 cut(s) 347, 743, 1073
BmiI GGNNCC 5 cut(s) 349, 577, 619, 736, 957
BmrFI CCNGG 7 cut(s) 101, 299, 345, 352, 416, 798, 1163
BmsI GCATC 3 cut(s) 121, 586, 783
BpmI CTGGAG 4 cut(s) 350, 636, 962, 1035
Bpu10I CCTNAGC 1 cut(s) 1181
BpuMI CCSGG 2 cut(s) 101, 299
BsaAI YACGTR 1 cut(s) 216
BsaBI GATNNNNATC 1 cut(s) 877
BsaHI GRCGYC 2 cut(s) 203, 478
BsaJI CCNNGG 8 cut(s) 224, 297, 350, 351, 415, 746, 886, 959
BsaXI ACNNNNNCTCC 6 cut(s) 205, 235, 609, 639, 882, 912
Bsc4I CCNNNNNNNGG 3 cut(s) 123, 549, 1058
Bse1I ACTGG 4 cut(s) 367, 619, 979, 1141
Bse3DI GCAATG 1 cut(s) 708
Bse8I GATNNNNATC 1 cut(s) 877
BseBI CCWGG 5 cut(s) 345, 352, 416, 798, 1163
BseDI CCNNGG 8 cut(s) 224, 297, 350, 351, 415, 746, 886, 959
BseGI GGATG 4 cut(s) 87, 187, 214, 774
BseJI GATNNNNATC 1 cut(s) 877
BseLI CCNNNNNNNGG 3 cut(s) 123, 549, 1058
BseMI GCAATG 1 cut(s) 708
BseMII CTCAG 2 cut(s) 571, 1167
BseNI ACTGG 4 cut(s) 367, 619, 979, 1141
BseXI GCAGC 4 cut(s) 352, 448, 1054, 1197
BsgI GTGCAG 1 cut(s) 128
BshFI GGCC 3 cut(s) 220, 885, 1074
BshNI GGYRCC 2 cut(s) 575, 955
BsiSI CCGG 2 cut(s) 100, 299
BslFI GGGAC 3 cut(s) 280, 333, 368
BslI CCNNNNNNNGG 3 cut(s) 123, 549, 1058
BsmFI GGGAC 3 cut(s) 280, 333, 368
BsmI GAATGC 1 cut(s) 759
BsnI GGCC 3 cut(s) 220, 885, 1074
Bsp143I GATC 3 cut(s) 273, 560, 872
BspACI CCGC 2 cut(s) 164, 382
BspANI GGCC 3 cut(s) 220, 885, 1074
BspCNI CTCAG 2 cut(s) 570, 1166
BspHI TCATGA 1 cut(s) 276
BspLI GGNNCC 5 cut(s) 349, 577, 619, 736, 957
BspT107I GGYRCC 2 cut(s) 575, 955
BsrDI GCAATG 1 cut(s) 708
BsrI ACTGG 4 cut(s) 367, 619, 979, 1141
BssECI CCNNGG 8 cut(s) 224, 297, 350, 351, 415, 746, 886, 959
BssMI GATC 3 cut(s) 273, 560, 872
BssNAI GTATAC 1 cut(s) 17
BssNI GRCGYC 2 cut(s) 203, 478
BssSI CACGAG 1 cut(s) 783
BssT1I CCWWGG 2 cut(s) 746, 959
Bst1107I GTATAC 1 cut(s) 17
Bst2BI CACGAG 1 cut(s) 783
Bst2UI CCWGG 5 cut(s) 345, 352, 416, 798, 1163
Bst4CI ACNGT 2 cut(s) 539, 1015
BstACI GRCGYC 2 cut(s) 203, 478
BstBAI YACGTR 1 cut(s) 216
BstC8I GCNNGC 4 cut(s) 688, 835, 935, 1072
BstDEI CTNAG 3 cut(s) 557, 1153, 1181
BstDSI CCRYGG 1 cut(s) 886
BstENI CCTNNNNNAGG 1 cut(s) 121
BstF5I GGATG 4 cut(s) 87, 187, 214, 774
BstHHI GCGC 1 cut(s) 706
BstKTI GATC 3 cut(s) 276, 563, 875
BstMBI GATC 3 cut(s) 273, 560, 872
BstMWI GCNNNNNNNGC 5 cut(s) 169, 486, 710, 1163, 1185
BstNI CCWGG 5 cut(s) 345, 352, 416, 798, 1163
BstNSI RCATGY 3 cut(s) 163, 489, 937
BstSCI CCNGG 7 cut(s) 99, 297, 343, 350, 414, 796, 1161
BstV1I GCAGC 4 cut(s) 352, 448, 1054, 1197
BstXI CCANNNNNNTGG 1 cut(s) 841
BstZ17I GTATAC 1 cut(s) 17
BsuI GTATCC 1 cut(s) 1155
BsuRI GGCC 3 cut(s) 220, 885, 1074
BtgI CCRYGG 1 cut(s) 886
BtsCI GGATG 4 cut(s) 87, 187, 214, 774
BtsI GCAGTG 1 cut(s) 764
BtsIMutI CAGTG 4 cut(s) 374, 544, 764, 986
Cac8I GCNNGC 4 cut(s) 688, 835, 935, 1072
CciI TCATGA 1 cut(s) 276
CfoI GCGC 1 cut(s) 706
Cfr13I GGNCC 3 cut(s) 347, 743, 1073
CseI GACGC 2 cut(s) 192, 486
Csp6I GTAC 1 cut(s) 576
CviAII CATG 5 cut(s) 160, 277, 486, 934, 1082
CviQI GTAC 1 cut(s) 576
DdeI CTNAG 3 cut(s) 557, 1153, 1181
DpnI GATC 3 cut(s) 275, 562, 874
DpnII GATC 3 cut(s) 273, 560, 872
DraIII CACNNNGTG 1 cut(s) 103
Eco130I CCWWGG 2 cut(s) 746, 959
Eco47I GGWCC 2 cut(s) 347, 743
Eco57I CTGAAG 5 cut(s) 5, 537, 853, 1005, 1082
EcoNI CCTNNNNNAGG 1 cut(s) 121
EcoRI GAATTC 1 cut(s) 511
EcoRII CCWGG 5 cut(s) 343, 350, 414, 796, 1161
EcoT14I CCWWGG 2 cut(s) 746, 959
ErhI CCWWGG 2 cut(s) 746, 959
FaeI CATG 5 cut(s) 163, 280, 489, 937, 1085
FaqI GGGAC 3 cut(s) 280, 333, 368
FatI CATG 5 cut(s) 159, 276, 485, 933, 1081
FblI GTMKAC 2 cut(s) 16, 475
Fnu4HI GCNGC 5 cut(s) 164, 341, 462, 1068, 1186
FokI GGATG 4 cut(s) 94, 194, 221, 761
Fsp4HI GCNGC 5 cut(s) 164, 341, 462, 1068, 1186
FspBI CTAG 1 cut(s) 960
GlaI GCGC 1 cut(s) 705
GluI GCNGC 5 cut(s) 164, 341, 462, 1068, 1186
GsuI CTGGAG 4 cut(s) 350, 636, 962, 1035
HaeIII GGCC 3 cut(s) 220, 885, 1074
HapII CCGG 2 cut(s) 100, 299
HgaI GACGC 2 cut(s) 192, 486
HhaI GCGC 1 cut(s) 706
Hin1I GRCGYC 2 cut(s) 203, 478
Hin1II CATG 5 cut(s) 163, 280, 489, 937, 1085
Hin6I GCGC 1 cut(s) 704
HinP1I GCGC 1 cut(s) 704
HindIII AAGCTT 1 cut(s) 429
HinfI GANTC 2 cut(s) 356, 385
HpaII CCGG 2 cut(s) 100, 299
HphI GGTGA 2 cut(s) 47, 663
Hpy166II GTNNAC 2 cut(s) 17, 476
Hpy188I TCNGA 7 cut(s) 208, 225, 510, 517, 560, 872, 924
Hpy188III TCNNGA 8 cut(s) 10, 23, 124, 277, 738, 906, 1027, 1052
Hpy8I GTNNAC 2 cut(s) 17, 476
Hpy99I CGWCG 1 cut(s) 208
HpyAV CCTTC 5 cut(s) 274, 371, 464, 923, 1168
HpyCH4III ACNGT 2 cut(s) 539, 1015
HpyCH4IV ACGT 2 cut(s) 215, 320
HpyCH4V TGCA 6 cut(s) 109, 258, 464, 489, 599, 757
HpyF10VI GCNNNNNNNGC 5 cut(s) 169, 486, 710, 1163, 1185
HpyF3I CTNAG 3 cut(s) 557, 1153, 1181
HpySE526I ACGT 2 cut(s) 215, 320
Hsp92I GRCGYC 2 cut(s) 203, 478
Hsp92II CATG 5 cut(s) 163, 280, 489, 937, 1085
HspAI GCGC 1 cut(s) 704
KpnI GGTACC 1 cut(s) 579
Kzo9I GATC 3 cut(s) 273, 560, 872
LmnI GCTCC 2 cut(s) 45, 617
Lsp1109I GCAGC 4 cut(s) 352, 448, 1054, 1197
LweI GCATC 3 cut(s) 121, 586, 783
MaeI CTAG 1 cut(s) 960
MaeII ACGT 2 cut(s) 215, 320
MaeIII GTNAC 4 cut(s) 93, 625, 669, 1009
MalI GATC 3 cut(s) 275, 562, 874
MboI GATC 3 cut(s) 273, 560, 872
MboII GAAGA 4 cut(s) 241, 530, 859, 1088
MluCI AATT 9 cut(s) 74, 195, 262, 511, 565, 581, 824, 838, 1030
MlyI GAGTC 2 cut(s) 350, 394
MmeI TCCRAC 1 cut(s) 186
MnlI CCTC 9 cut(s) 48, 84, 127, 171, 193, 219, 231, 734, 794
MseI TTAA 2 cut(s) 422, 944
MslI CAYNNNNRTG 2 cut(s) 961, 1086
MspA1I CMGCKG 1 cut(s) 1039
MspI CCGG 2 cut(s) 100, 299
MspR9I CCNGG 7 cut(s) 101, 299, 345, 352, 416, 798, 1163
Mva1269I GAATGC 1 cut(s) 759
MvaI CCWGG 5 cut(s) 345, 352, 416, 798, 1163
MwoI GCNNNNNNNGC 5 cut(s) 169, 486, 710, 1163, 1185
NciI CCSGG 2 cut(s) 101, 299
NdeII GATC 3 cut(s) 273, 560, 872
NlaIII CATG 5 cut(s) 163, 280, 489, 937, 1085
NlaIV GGNNCC 5 cut(s) 349, 577, 619, 736, 957
NmuCI GTSAC 4 cut(s) 93, 625, 669, 1009
NspI RCATGY 3 cut(s) 163, 489, 937
OliI CACNNNNGTG 1 cut(s) 961
PaeI GCATGC 1 cut(s) 937
PagI TCATGA 1 cut(s) 276
PasI CCCWGGG 1 cut(s) 351
PcsI WCGNNNNNNNCGW 2 cut(s) 212, 221
PctI GAATGC 1 cut(s) 759
PkrI GCNGC 5 cut(s) 165, 342, 463, 1069, 1187
PleI GAGTC 2 cut(s) 350, 393
PpsI GAGTC 2 cut(s) 350, 393
Ppu21I YACGTR 1 cut(s) 216
Psp6I CCWGG 5 cut(s) 343, 350, 414, 796, 1161
PspGI CCWGG 5 cut(s) 343, 350, 414, 796, 1161
PspN4I GGNNCC 5 cut(s) 349, 577, 619, 736, 957
PspPI GGNCC 3 cut(s) 347, 743, 1073
PvuII CAGCTG 1 cut(s) 1039
RsaI GTAC 1 cut(s) 577
RsaNI GTAC 1 cut(s) 576
RseI CAYNNNNRTG 2 cut(s) 961, 1086
SaqAI TTAA 2 cut(s) 422, 944
SatI GCNGC 5 cut(s) 164, 341, 462, 1068, 1186
Sau3AI GATC 3 cut(s) 273, 560, 872
Sau96I GGNCC 3 cut(s) 347, 743, 1073
SchI GAGTC 2 cut(s) 350, 394
ScrFI CCNGG 7 cut(s) 101, 299, 345, 352, 416, 798, 1163
SfaNI GCATC 3 cut(s) 121, 586, 783
SinI GGWCC 2 cut(s) 347, 743
SmiMI CAYNNNNRTG 2 cut(s) 961, 1086
SphI GCATGC 1 cut(s) 937
Sse9I AATT 9 cut(s) 74, 195, 262, 511, 565, 581, 824, 838, 1030
SsiI CCGC 2 cut(s) 164, 382
SspMI CTAG 1 cut(s) 960
StyD4I CCNGG 7 cut(s) 99, 297, 343, 350, 414, 796, 1161
StyI CCWWGG 2 cut(s) 746, 959
TaaI ACNGT 2 cut(s) 539, 1015
TaiI ACGT 2 cut(s) 218, 323
TaqI TCGA 2 cut(s) 53, 89
TasI AATT 9 cut(s) 74, 195, 262, 511, 565, 581, 824, 838, 1030
TauI GCSGC 1 cut(s) 166
Tru1I TTAA 2 cut(s) 422, 944
Tru9I TTAA 2 cut(s) 422, 944
TscAI CASTG 4 cut(s) 374, 544, 764, 986
TseFI GTSAC 4 cut(s) 93, 625, 669, 1009
TseI GCWGC 4 cut(s) 340, 461, 1067, 1185
Tsp45I GTSAC 4 cut(s) 93, 625, 669, 1009
TspDTI ATGAA 5 cut(s) 44, 98, 293, 674, 942
TspGWI ACGGA 1 cut(s) 1074
TspRI CASTG 4 cut(s) 374, 544, 764, 986
VpaK11BI GGWCC 2 cut(s) 347, 743
XagI CCTNNNNNAGG 1 cut(s) 121
XapI RAATTY 6 cut(s) 74, 511, 565, 581, 824, 1030
XceI RCATGY 3 cut(s) 163, 489, 937
XmaJI CCTAGG 1 cut(s) 959
XmiI GTMKAC 2 cut(s) 16, 475
XspI CTAG 1 cut(s) 960
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.