RchiOBHm_Chr5g0065161

Tubby-like F-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0001645
Physical Location & Seq
Forward (+)
71042695 .. 71043894
1200 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ34106

Sequence Viewer

Length: 1200 bp
ATGTCTTTCAAGAGTATACTTCAGGATAAGAAAGGCAAGTTTGGAAGCAGTTGGAGGATACGTCTTAATCTGAAATTTAGTTATGGGATGATATCTAGGTCTCACCGGGTGGTTCAGGACACCTCTTTCAGGATTGATGCTTTGAAGCAGAGTTGTTGGGCTAACGTGCCGCCGGAGCTTTTGAGGGAGGTGTTGATGAGAATTGAGGCTTCTGATGATACATGGCCTCTGCGGAAAAATGTGGTCGCTTGTGCTGGTGTGTGCAACAATTGGAGGGAGATGATGAAAGAGATTGTGAAGTCCCCAGAAGTTTCGGGCAAGCTGACATTCCCAATCTCCTTGAAGCAGCCTGGTCCCAGGGACTTCCTTCTCCAATGTTATATAAAGCGTAGCAACCAGGCATATTATCTTTTCCTGGGCTTAAATCAAGCTTCAACTGATGATGGGAAGTTCCTTCTTGCTGCGAGAAAGCGTAGACACCTAACATGCACAGATTACATCATCTCTCTGAATTCTGAAAACGTGTCAAAAGGGAACAGTGCCTTTGTTGGAAAGCTCAGATCAAATTTTCTGGGTACCAAATTTACAATCTATGATGCGCAACCAAGTAATACTGGAGCCAATGTCACTAAATTTCATTCCACCATGAAACAAGTGTCACCCAGGGTTCCTGCTGGCGACTATCCTGTGGGCCACATTGCTTATGAGTTGAACGTCTTGGGTTCCAGAGGTTCAAGGAGAATGAAGTGCGTATTGGATGCCATCCCTGCTCGTGCTGTTGAGCCAGGAGGTGCCCCTACGCAGACTGAATTTGTCGATAGCAATTCGGATAGTACTCCATCTCTCCCTCTCTTCGGATCAAAATCAGGTCCCGCGGAGAGTGTCCAATCTGGACAGCAATCTGGTCAGAATGAAGGGATGCTGGTATTAAGGAGCAAGGCACCTAGGTGGCTCGAACAACTCCAGCGCTGGTGCTTAAACTTCAATCGACGAGTAACTGAAGCTTCAGTCAAGAATTTTCAGCTGGTTGCTTCTCCAGAGAACGGAGTTGCTGCTGACCCAGAGCATGAGAATGTCATTCTCGAGTGTGGAAAAGTAGGCAAAGATGTATTCACAATGGATTACCAGTATCCAATCTCAGCCTTCCAGGCATTTGCTATTTGCCTTAGCAGCATTGACTCCAAGATTGCTTGTGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005543 GO:0008289 GO:0035091 GO:0043167 GO:0043168
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box Tub

Protein Analysis

399

Amino Acids

44.41

Weight (kDa)

9.32

Isoelectric Point (pI)

53.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tub PF01167 114 - 394 5.3e-94 Tub family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 600
Acc65I GGTACC 1 cut(s) 575
AccB1I GGYRCC 3 cut(s) 575, 791, 940
AccI GTMKAC 2 cut(s) 16, 475
AccII CGCG 1 cut(s) 875
AciI CCGC 4 cut(s) 170, 232, 873, 875
AclWI GGATC 1 cut(s) 865
AcsI RAATTY 7 cut(s) 74, 511, 565, 581, 632, 809, 1015
AcuI CTGAAG 3 cut(s) 5, 990, 1020
AdeI CACNNNGTG 1 cut(s) 109
AfaI GTAC 2 cut(s) 577, 835
AfeI AGCGCT 1 cut(s) 968
AfiI CCNNNNNNNGG 3 cut(s) 129, 854, 1043
AflIII ACRYGT 1 cut(s) 522
AgsI TTSAA 7 cut(s) 10, 145, 343, 435, 712, 735, 985
AjnI CCWGG 7 cut(s) 349, 356, 396, 414, 662, 784, 1146
AjuI GAANNNNNNNTTGG 2 cut(s) 737, 769
AleI CACNNNNGTG 1 cut(s) 946
AluBI AGCT 6 cut(s) 178, 322, 431, 556, 1004, 1024
AluI AGCT 6 cut(s) 178, 322, 431, 556, 1004, 1024
Alw26I GTCTC 1 cut(s) 105
AlwI GGATC 1 cut(s) 865
Ama87I CYCGRG 1 cut(s) 1082
Aor51HI AGCGCT 1 cut(s) 968
AoxI GGCC 2 cut(s) 224, 691
ApeKI GCWGC 4 cut(s) 346, 461, 1052, 1170
ApoI RAATTY 7 cut(s) 74, 511, 565, 581, 632, 809, 1015
Asp718I GGTACC 1 cut(s) 575
AspA2I CCTAGG 1 cut(s) 944
AspLEI GCGC 2 cut(s) 601, 969
AspS9I GGNCC 3 cut(s) 353, 691, 869
AsuC2I CCSGG 1 cut(s) 107
AsuHPI GGTGA 2 cut(s) 95, 651
AvaI CYCGRG 1 cut(s) 1082
AvaII GGWCC 2 cut(s) 353, 869
AvrII CCTAGG 1 cut(s) 944
BaeGI GKGCMC 1 cut(s) 796
BanI GGYRCC 3 cut(s) 575, 791, 940
BauI CACGAG 1 cut(s) 771
BbvI GCAGC 4 cut(s) 358, 448, 1039, 1182
BccI CCATC 3 cut(s) 437, 770, 847
BciT130I CCWGG 7 cut(s) 351, 358, 398, 416, 664, 786, 1148
BciVI GTATCC 2 cut(s) 51, 1140
BcnI CCSGG 1 cut(s) 107
BcoDI GTCTC 1 cut(s) 105
BfaI CTAG 2 cut(s) 96, 945
BfoI RGCGCY 1 cut(s) 970
BfuI GTATCC 2 cut(s) 51, 1140
BglI GCCNNNNNGGC 1 cut(s) 1148
BisI GCNGC 5 cut(s) 170, 347, 462, 1053, 1171
BlnI CCTAGG 1 cut(s) 944
BlsI GCNGC 5 cut(s) 171, 348, 463, 1054, 1172
BmcAI AGTACT 1 cut(s) 835
Bme1390I CCNGG 8 cut(s) 107, 351, 358, 398, 416, 664, 786, 1148
Bme18I GGWCC 2 cut(s) 353, 869
BmeT110I CYCGRG 1 cut(s) 1082
BmgT120I GGNCC 3 cut(s) 353, 691, 869
BmiI GGNNCC 8 cut(s) 355, 577, 619, 669, 724, 793, 871, 942
BmrFI CCNGG 8 cut(s) 107, 351, 358, 398, 416, 664, 786, 1148
BmsI GCATC 4 cut(s) 127, 586, 748, 909
BpmI CTGGAG 3 cut(s) 636, 947, 1020
Bpu10I CCTNAGC 1 cut(s) 1166
BpuMI CCSGG 1 cut(s) 107
BsaBI GATNNNNATC 1 cut(s) 862
BsaI GGTCTC 1 cut(s) 105
BsaJI CCNNGG 7 cut(s) 356, 357, 415, 662, 663, 873, 944
BsaXI ACNNNNNCTCC 4 cut(s) 46, 76, 609, 639
Bsc4I CCNNNNNNNGG 3 cut(s) 129, 854, 1043
Bse1I ACTGG 2 cut(s) 619, 1126
Bse3DI GCAATG 1 cut(s) 696
Bse8I GATNNNNATC 1 cut(s) 862
BseBI CCWGG 7 cut(s) 351, 358, 398, 416, 664, 786, 1148
BseDI CCNNGG 7 cut(s) 356, 357, 415, 662, 663, 873, 944
BseGI GGATG 4 cut(s) 93, 762, 763, 924
BseJI GATNNNNATC 1 cut(s) 862
BseLI CCNNNNNNNGG 3 cut(s) 129, 854, 1043
BseMI GCAATG 1 cut(s) 696
BseMII CTCAG 2 cut(s) 571, 1152
BseNI ACTGG 2 cut(s) 619, 1126
BseSI GKGCMC 1 cut(s) 796
BseXI GCAGC 4 cut(s) 358, 448, 1039, 1182
Bsh1236I CGCG 1 cut(s) 875
BshFI GGCC 2 cut(s) 226, 693
BshNI GGYRCC 3 cut(s) 575, 791, 940
BsiHKCI CYCGRG 1 cut(s) 1082
BsiSI CCGG 2 cut(s) 106, 173
BslFI GGGAC 4 cut(s) 286, 339, 374, 855
BslI CCNNNNNNNGG 3 cut(s) 129, 854, 1043
BsmAI GTCTC 1 cut(s) 105
BsmFI GGGAC 4 cut(s) 286, 339, 374, 855
BsnI GGCC 2 cut(s) 226, 693
Bso31I GGTCTC 1 cut(s) 105
BsoBI CYCGRG 1 cut(s) 1082
Bsp1286I GDGCHC 1 cut(s) 796
Bsp143I GATC 2 cut(s) 560, 857
BspACI CCGC 4 cut(s) 170, 232, 873, 875
BspANI GGCC 2 cut(s) 226, 693
BspCNI CTCAG 2 cut(s) 570, 1151
BspFNI CGCG 1 cut(s) 875
BspLI GGNNCC 8 cut(s) 355, 577, 619, 669, 724, 793, 871, 942
BspPI GGATC 1 cut(s) 865
BspT107I GGYRCC 3 cut(s) 575, 791, 940
BspTNI GGTCTC 1 cut(s) 105
BsrDI GCAATG 1 cut(s) 696
BsrI ACTGG 2 cut(s) 619, 1126
BssECI CCNNGG 7 cut(s) 356, 357, 415, 662, 663, 873, 944
BssMI GATC 2 cut(s) 560, 857
BssNAI GTATAC 1 cut(s) 17
BssSI CACGAG 1 cut(s) 771
BssT1I CCWWGG 1 cut(s) 944
Bst1107I GTATAC 1 cut(s) 17
Bst2BI CACGAG 1 cut(s) 771
Bst2UI CCWGG 7 cut(s) 351, 358, 398, 416, 664, 786, 1148
Bst4CI ACNGT 1 cut(s) 539
Bst6I CTCTTC 1 cut(s) 857
BstC8I GCNNGC 2 cut(s) 320, 676
BstDEI CTNAG 3 cut(s) 557, 1138, 1166
BstDSI CCRYGG 1 cut(s) 873
BstENI CCTNNNNNAGG 1 cut(s) 127
BstF5I GGATG 4 cut(s) 93, 762, 763, 924
BstFNI CGCG 1 cut(s) 875
BstH2I RGCGCY 1 cut(s) 970
BstHHI GCGC 2 cut(s) 601, 969
BstKTI GATC 2 cut(s) 563, 860
BstMAI GTCTC 1 cut(s) 105
BstMBI GATC 2 cut(s) 560, 857
BstMWI GCNNNNNNNGC 4 cut(s) 175, 767, 1148, 1170
BstNI CCWGG 7 cut(s) 351, 358, 398, 416, 664, 786, 1148
BstNSI RCATGY 1 cut(s) 489
BstSCI CCNGG 8 cut(s) 105, 349, 356, 396, 414, 662, 784, 1146
BstSLI GKGCMC 1 cut(s) 796
BstUI CGCG 1 cut(s) 875
BstV1I GCAGC 4 cut(s) 358, 448, 1039, 1182
BstZ17I GTATAC 1 cut(s) 17
BsuI GTATCC 2 cut(s) 51, 1140
BsuRI GGCC 2 cut(s) 226, 693
BtgI CCRYGG 1 cut(s) 873
BtsCI GGATG 4 cut(s) 93, 762, 763, 924
BtsIMutI CAGTG 1 cut(s) 544
Cac8I GCNNGC 2 cut(s) 320, 676
CfoI GCGC 2 cut(s) 601, 969
Cfr13I GGNCC 3 cut(s) 353, 691, 869
Cfr42I CCGCGG 1 cut(s) 876
Csp6I GTAC 2 cut(s) 576, 834
CviAII CATG 4 cut(s) 222, 486, 646, 1067
CviQI GTAC 2 cut(s) 576, 834
DdeI CTNAG 3 cut(s) 557, 1138, 1166
DpnI GATC 2 cut(s) 562, 859
DpnII GATC 2 cut(s) 560, 857
DraIII CACNNNGTG 1 cut(s) 109
Eam1104I CTCTTC 1 cut(s) 857
EarI CTCTTC 1 cut(s) 857
Eco130I CCWWGG 1 cut(s) 944
Eco31I GGTCTC 1 cut(s) 105
Eco32I GATATC 1 cut(s) 93
Eco47I GGWCC 2 cut(s) 353, 869
Eco47III AGCGCT 1 cut(s) 968
Eco57I CTGAAG 3 cut(s) 5, 990, 1020
Eco88I CYCGRG 1 cut(s) 1082
EcoNI CCTNNNNNAGG 1 cut(s) 127
EcoO109I RGGNCCY 1 cut(s) 869
EcoRI GAATTC 1 cut(s) 511
EcoRII CCWGG 7 cut(s) 349, 356, 396, 414, 662, 784, 1146
EcoRV GATATC 1 cut(s) 93
EcoT14I CCWWGG 1 cut(s) 944
ErhI CCWWGG 1 cut(s) 944
FaeI CATG 4 cut(s) 225, 489, 649, 1070
FaqI GGGAC 4 cut(s) 286, 339, 374, 855
FatI CATG 4 cut(s) 221, 485, 645, 1066
FauI CCCGC 1 cut(s) 880
FblI GTMKAC 2 cut(s) 16, 475
Fnu4HI GCNGC 5 cut(s) 170, 347, 462, 1053, 1171
FokI GGATG 4 cut(s) 100, 749, 770, 931
Fsp4HI GCNGC 5 cut(s) 170, 347, 462, 1053, 1171
FspBI CTAG 2 cut(s) 96, 945
FspI TGCGCA 1 cut(s) 600
GlaI GCGC 2 cut(s) 600, 968
GluI GCNGC 5 cut(s) 170, 347, 462, 1053, 1171
GsuI CTGGAG 3 cut(s) 636, 947, 1020
HaeII RGCGCY 1 cut(s) 970
HaeIII GGCC 2 cut(s) 226, 693
HapII CCGG 2 cut(s) 106, 173
HhaI GCGC 2 cut(s) 601, 969
Hin1II CATG 4 cut(s) 225, 489, 649, 1070
Hin6I GCGC 2 cut(s) 599, 967
HinP1I GCGC 2 cut(s) 599, 967
HindIII AAGCTT 2 cut(s) 429, 1002
HinfI GANTC 1 cut(s) 1178
HpaII CCGG 2 cut(s) 106, 173
HphI GGTGA 2 cut(s) 95, 651
Hpy166II GTNNAC 2 cut(s) 17, 476
Hpy188I TCNGA 8 cut(s) 72, 214, 510, 517, 560, 829, 857, 909
Hpy188III TCNNGA 9 cut(s) 10, 23, 116, 130, 726, 891, 1012, 1037, 1082
Hpy8I GTNNAC 2 cut(s) 17, 476
Hpy99I CGWCG 1 cut(s) 993
HpyAV CCTTC 4 cut(s) 377, 464, 908, 1153
HpyCH4III ACNGT 1 cut(s) 539
HpyCH4IV ACGT 4 cut(s) 61, 165, 522, 714
HpyCH4V TGCA 2 cut(s) 264, 489
HpyF10VI GCNNNNNNNGC 4 cut(s) 175, 767, 1148, 1170
HpyF3I CTNAG 3 cut(s) 557, 1138, 1166
HpySE526I ACGT 4 cut(s) 61, 165, 522, 714
Hsp92II CATG 4 cut(s) 225, 489, 649, 1070
HspAI GCGC 2 cut(s) 599, 967
KpnI GGTACC 1 cut(s) 579
KspI CCGCGG 1 cut(s) 876
Kzo9I GATC 2 cut(s) 560, 857
LmnI GCTCC 3 cut(s) 175, 617, 933
Lsp1109I GCAGC 4 cut(s) 358, 448, 1039, 1182
LweI GCATC 4 cut(s) 127, 586, 748, 909
MaeI CTAG 2 cut(s) 96, 945
MaeII ACGT 4 cut(s) 61, 165, 522, 714
MaeIII GTNAC 3 cut(s) 625, 657, 994
MalI GATC 2 cut(s) 562, 859
MboI GATC 2 cut(s) 560, 857
MboII GAAGA 1 cut(s) 844
MfeI CAATTG 1 cut(s) 268
MhlI GDGCHC 1 cut(s) 796
MlyI GAGTC 1 cut(s) 1172
MmeI TCCRAC 2 cut(s) 32, 529
MseI TTAA 4 cut(s) 66, 422, 929, 977
MslI CAYNNNNRTG 2 cut(s) 946, 1071
MspA1I CMGCKG 2 cut(s) 875, 1024
MspI CCGG 2 cut(s) 106, 173
MspR9I CCNGG 8 cut(s) 107, 351, 358, 398, 416, 664, 786, 1148
MunI CAATTG 1 cut(s) 268
MvaI CCWGG 7 cut(s) 351, 358, 398, 416, 664, 786, 1148
MvnI CGCG 1 cut(s) 875
MwoI GCNNNNNNNGC 4 cut(s) 175, 767, 1148, 1170
NciI CCSGG 1 cut(s) 107
NdeII GATC 2 cut(s) 560, 857
NlaIII CATG 4 cut(s) 225, 489, 649, 1070
NlaIV GGNNCC 8 cut(s) 355, 577, 619, 669, 724, 793, 871, 942
NmuCI GTSAC 2 cut(s) 625, 657
NsbI TGCGCA 1 cut(s) 600
NspI RCATGY 1 cut(s) 489
OliI CACNNNNGTG 1 cut(s) 946
PaeR7I CTCGAG 1 cut(s) 1082
PasI CCCWGGG 2 cut(s) 357, 663
PkrI GCNGC 5 cut(s) 171, 348, 463, 1054, 1172
PleI GAGTC 1 cut(s) 1172
PpsI GAGTC 1 cut(s) 1172
PpuMI RGGWCCY 1 cut(s) 869
Psp5II RGGWCCY 1 cut(s) 869
Psp6I CCWGG 7 cut(s) 349, 356, 396, 414, 662, 784, 1146
PspGI CCWGG 7 cut(s) 349, 356, 396, 414, 662, 784, 1146
PspN4I GGNNCC 8 cut(s) 355, 577, 619, 669, 724, 793, 871, 942
PspPI GGNCC 3 cut(s) 353, 691, 869
PspPPI RGGWCCY 1 cut(s) 869
PvuII CAGCTG 1 cut(s) 1024
RsaI GTAC 2 cut(s) 577, 835
RsaNI GTAC 2 cut(s) 576, 834
RseI CAYNNNNRTG 2 cut(s) 946, 1071
SacII CCGCGG 1 cut(s) 876
SaqAI TTAA 4 cut(s) 66, 422, 929, 977
SatI GCNGC 5 cut(s) 170, 347, 462, 1053, 1171
Sau3AI GATC 2 cut(s) 560, 857
Sau96I GGNCC 3 cut(s) 353, 691, 869
ScaI AGTACT 1 cut(s) 835
SchI GAGTC 1 cut(s) 1172
ScrFI CCNGG 8 cut(s) 107, 351, 358, 398, 416, 664, 786, 1148
SduI GDGCHC 1 cut(s) 796
SfaNI GCATC 4 cut(s) 127, 586, 748, 909
Sfr274I CTCGAG 1 cut(s) 1082
Sfr303I CCGCGG 1 cut(s) 876
SgrBI CCGCGG 1 cut(s) 876
SinI GGWCC 2 cut(s) 353, 869
SlaI CTCGAG 1 cut(s) 1082
SmiMI CAYNNNNRTG 2 cut(s) 946, 1071
SmlI CTYRAG 1 cut(s) 1082
SmoI CTYRAG 1 cut(s) 1082
SsiI CCGC 4 cut(s) 170, 232, 873, 875
SspMI CTAG 2 cut(s) 96, 945
StyD4I CCNGG 8 cut(s) 105, 349, 356, 396, 414, 662, 784, 1146
StyI CCWWGG 1 cut(s) 944
TaaI ACNGT 1 cut(s) 539
TaiI ACGT 4 cut(s) 64, 168, 525, 717
TaqI TCGA 4 cut(s) 816, 954, 988, 1083
TatI WGTACW 1 cut(s) 833
TauI GCSGC 1 cut(s) 172
Tru1I TTAA 4 cut(s) 66, 422, 929, 977
Tru9I TTAA 4 cut(s) 66, 422, 929, 977
TscAI CASTG 1 cut(s) 544
TseFI GTSAC 2 cut(s) 625, 657
TseI GCWGC 4 cut(s) 346, 461, 1052, 1170
Tsp45I GTSAC 2 cut(s) 625, 657
TspDTI ATGAA 5 cut(s) 299, 626, 662, 758, 927
TspGWI ACGGA 1 cut(s) 1059
TspRI CASTG 1 cut(s) 544
VpaK11BI GGWCC 2 cut(s) 353, 869
XagI CCTNNNNNAGG 1 cut(s) 127
XapI RAATTY 7 cut(s) 74, 511, 565, 581, 632, 809, 1015
XceI RCATGY 1 cut(s) 489
XhoI CTCGAG 1 cut(s) 1082
XmaJI CCTAGG 1 cut(s) 944
XmiI GTMKAC 2 cut(s) 16, 475
XspI CTAG 2 cut(s) 96, 945
ZrmI AGTACT 1 cut(s) 835
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.