Rroxscaffold_7G00174860

Tubby-like F-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0001645
Physical Location & Seq
Forward (+)
14551525 .. 14554818
3294 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00174860.1

Sequence Viewer

Length: 1221 bp
ATGTCTTTCAAGAGTATACTTCAGGATATGAAAGGTGAGTTTGGGAGCATTTCGAGGAAAGGGTTTGATGTGAAATTTGGGTATGGGATGAGATCGAGGTCTCACCGGGTGGTTCAGGATACCTCGTTCAGGATTGATGCTTTGAAGCAGAGTTGTTGGGCTAACATGCCGCCGGAGCTTTTGAGGGATGTGTTGATGAGAATTGAGGCGTCTGATGATACATGGCCGCCGCGGAAGAATGTGGTCGCTTGTGCTGGTGTGTGCAAGAATTGGAGAGAGATCATGAAAGAGATTGTCAAGTCCCTAGAAGTTTCGGGGAAGCTGACGTTTCCAATCTCCTTGAAGCAGCCTGGTCCCAGGGATTCCCTTCTTCAGTGCTATATAAAGCGGAGACGTAGCAACCAGACATATTATCTTTTCCTGGGCTTAAATCAAGCTTCAACTGATGATGGGAAGTTCCTTCTTGCTGCGAGAAAGTGTAGACGCCCAACATGCACAGATTATATCATCTCTCTGAATTCTGAAGATGTGTCAAAAGGGAACAGTGCCTTTGTTGGAAAGCTCAGATCAAATTTTCTGGGAACCAAATTCACAATCTATGATGCCCAACCAACTAATACTGGAGCCAAAGTCACTAAATGTCATTCCTCCAGACTTGTAAATATGAAACAAGTGTCACCCAGAGTTCCTGCTGGCAACTATCCTGTGGCCCACATTGCTTATGAGTTGAATGTCTTGGGTTCCAGAGGTCCAAGGAGAATGCAGTGTGTAATGGATGCCATCCCTGCCCGTGCTGTTGAGCCAGGAGGTGTGGCCCCTACGCAGACTGAATTTGTTGATAGCAATTTGGATAGTATTCCATCTCTCCCTTTCATCAGATCAAAATCAGGTCATGCAGAGAGTGTCCAATCTGGGCTACAATCTAGTCAGAATGAAGGCATGCTGGTATTAAGGAACAAGGCACCTAGGTGGCACGAACAACTCCAGTGCTGGTGTCTGAACTTCAATGGACGAGTAACCGTTGCTTCAGTCAAGAATTTTCAGCTGGTTGCTTCTCCAGAGAACGGAGTAGCTGCTGGCCCAGAGCATGAGAATGTCATTCTCCAGTTTGGAAAAGTTGGCAAAGATGTATTCACAATGGATTACCAGTATCCAATCTCAGCCTTCCAGGCATTCGCTATTTGCCTTAGCAGCTTTGACACCAAGATTGCTTGTGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005543 GO:0008289 GO:0035091 GO:0043167 GO:0043168
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box Tub

Protein Analysis

406

Amino Acids

45.23

Weight (kDa)

9.41

Isoelectric Point (pI)

54.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tub PF01167 114 - 401 4e-108 Tub family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 961
AccI GTMKAC 2 cut(s) 16, 481
AccII CGCG 1 cut(s) 232
AciI CCGC 5 cut(s) 170, 227, 230, 232, 388
AcoI YGGCCR 1 cut(s) 224
AcsI RAATTY 6 cut(s) 74, 517, 571, 587, 830, 1036
AcuI CTGAAG 4 cut(s) 5, 356, 543, 1011
AcyI GRCGYC 2 cut(s) 209, 484
AdeI CACNNNGTG 1 cut(s) 109
AfiI CCNNNNNNNGG 2 cut(s) 129, 1064
AgsI TTSAA 6 cut(s) 10, 145, 343, 441, 730, 1006
AjnI CCWGG 5 cut(s) 349, 356, 420, 802, 1167
AleI CACNNNNGTG 1 cut(s) 967
AloI GAACNNNNNNTCC 2 cut(s) 110, 142
AluBI AGCT 7 cut(s) 178, 322, 437, 562, 1045, 1073, 1194
AluI AGCT 7 cut(s) 178, 322, 437, 562, 1045, 1073, 1194
Alw26I GTCTC 2 cut(s) 105, 385
AoxI GGCC 4 cut(s) 224, 708, 813, 1078
ApeKI GCWGC 4 cut(s) 346, 467, 1073, 1191
ApoI RAATTY 6 cut(s) 74, 517, 571, 587, 830, 1036
AspA2I CCTAGG 1 cut(s) 965
AspS9I GGNCC 5 cut(s) 353, 709, 749, 814, 1079
AsuC2I CCSGG 1 cut(s) 107
AsuHPI GGTGA 3 cut(s) 47, 95, 669
AvaII GGWCC 2 cut(s) 353, 749
AvrII CCTAGG 1 cut(s) 965
BanI GGYRCC 1 cut(s) 961
BbvI GCAGC 4 cut(s) 358, 454, 1060, 1203
BccI CCATC 3 cut(s) 443, 788, 868
BciT130I CCWGG 5 cut(s) 351, 358, 422, 804, 1169
BciVI GTATCC 2 cut(s) 112, 1161
BcnI CCSGG 1 cut(s) 107
BcoDI GTCTC 2 cut(s) 105, 385
BfaI CTAG 3 cut(s) 305, 924, 966
BfuI GTATCC 2 cut(s) 112, 1161
BglI GCCNNNNNGGC 1 cut(s) 1169
BisI GCNGC 7 cut(s) 170, 227, 230, 347, 468, 1074, 1192
BlnI CCTAGG 1 cut(s) 965
BlsI GCNGC 7 cut(s) 171, 228, 231, 348, 469, 1075, 1193
Bme1390I CCNGG 6 cut(s) 107, 351, 358, 422, 804, 1169
Bme18I GGWCC 2 cut(s) 353, 749
BmgT120I GGNCC 5 cut(s) 353, 709, 749, 814, 1079
BmiI GGNNCC 6 cut(s) 355, 583, 625, 742, 816, 963
BmrFI CCNGG 6 cut(s) 107, 351, 358, 422, 804, 1169
BmsI GCATC 3 cut(s) 127, 592, 766
BpmI CTGGAG 5 cut(s) 634, 642, 968, 1041, 1088
Bpu10I CCTNAGC 1 cut(s) 1187
BpuMI CCSGG 1 cut(s) 107
BsaBI GATNNNNATC 1 cut(s) 883
BsaHI GRCGYC 2 cut(s) 209, 484
BsaI GGTCTC 1 cut(s) 105
BsaJI CCNNGG 6 cut(s) 230, 356, 357, 421, 752, 965
BsaXI ACNNNNNCTCC 2 cut(s) 615, 645
Bsc4I CCNNNNNNNGG 2 cut(s) 129, 1064
Bse1I ACTGG 4 cut(s) 625, 985, 1105, 1147
Bse3DI GCAATG 1 cut(s) 714
Bse8I GATNNNNATC 1 cut(s) 883
BseBI CCWGG 5 cut(s) 351, 358, 422, 804, 1169
BseDI CCNNGG 6 cut(s) 230, 356, 357, 421, 752, 965
BseGI GGATG 4 cut(s) 93, 193, 780, 781
BseJI GATNNNNATC 1 cut(s) 883
BseLI CCNNNNNNNGG 2 cut(s) 129, 1064
BseMI GCAATG 1 cut(s) 714
BseMII CTCAG 2 cut(s) 577, 1173
BseNI ACTGG 4 cut(s) 625, 985, 1105, 1147
BseXI GCAGC 4 cut(s) 358, 454, 1060, 1203
Bsh1236I CGCG 1 cut(s) 232
BshFI GGCC 4 cut(s) 226, 710, 815, 1080
BshNI GGYRCC 1 cut(s) 961
BsiSI CCGG 2 cut(s) 106, 173
BslFI GGGAC 2 cut(s) 286, 339
BslI CCNNNNNNNGG 2 cut(s) 129, 1064
BsmAI GTCTC 2 cut(s) 105, 385
BsmBI CGTCTC 1 cut(s) 385
BsmFI GGGAC 2 cut(s) 286, 339
BsmI GAATGC 2 cut(s) 765, 1172
BsnI GGCC 4 cut(s) 226, 710, 815, 1080
Bso31I GGTCTC 1 cut(s) 105
Bsp143I GATC 4 cut(s) 92, 279, 566, 878
BspACI CCGC 5 cut(s) 170, 227, 230, 232, 388
BspANI GGCC 4 cut(s) 226, 710, 815, 1080
BspCNI CTCAG 2 cut(s) 576, 1172
BspFNI CGCG 1 cut(s) 232
BspHI TCATGA 1 cut(s) 282
BspLI GGNNCC 6 cut(s) 355, 583, 625, 742, 816, 963
BspT107I GGYRCC 1 cut(s) 961
BspTNI GGTCTC 1 cut(s) 105
BsrDI GCAATG 1 cut(s) 714
BsrI ACTGG 4 cut(s) 625, 985, 1105, 1147
BssECI CCNNGG 6 cut(s) 230, 356, 357, 421, 752, 965
BssMI GATC 4 cut(s) 92, 279, 566, 878
BssNAI GTATAC 1 cut(s) 17
BssNI GRCGYC 2 cut(s) 209, 484
BssT1I CCWWGG 2 cut(s) 752, 965
Bst1107I GTATAC 1 cut(s) 17
Bst2UI CCWGG 5 cut(s) 351, 358, 422, 804, 1169
Bst4CI ACNGT 2 cut(s) 545, 1021
BstACI GRCGYC 2 cut(s) 209, 484
BstC8I GCNNGC 3 cut(s) 694, 941, 1078
BstDEI CTNAG 3 cut(s) 563, 1159, 1187
BstDSI CCRYGG 1 cut(s) 230
BstENI CCTNNNNNAGG 1 cut(s) 127
BstF5I GGATG 4 cut(s) 93, 193, 780, 781
BstFNI CGCG 1 cut(s) 232
BstKTI GATC 4 cut(s) 95, 282, 569, 881
BstMAI GTCTC 2 cut(s) 105, 385
BstMBI GATC 4 cut(s) 92, 279, 566, 878
BstMWI GCNNNNNNNGC 6 cut(s) 175, 492, 716, 785, 1169, 1191
BstNI CCWGG 5 cut(s) 351, 358, 422, 804, 1169
BstNSI RCATGY 3 cut(s) 169, 495, 943
BstSCI CCNGG 6 cut(s) 105, 349, 356, 420, 802, 1167
BstUI CGCG 1 cut(s) 232
BstV1I GCAGC 4 cut(s) 358, 454, 1060, 1203
BstZ17I GTATAC 1 cut(s) 17
BsuI GTATCC 2 cut(s) 112, 1161
BsuRI GGCC 4 cut(s) 226, 710, 815, 1080
BtgI CCRYGG 1 cut(s) 230
BtsCI GGATG 4 cut(s) 93, 193, 780, 781
BtsI GCAGTG 1 cut(s) 770
BtsIMutI CAGTG 4 cut(s) 380, 550, 770, 992
Cac8I GCNNGC 3 cut(s) 694, 941, 1078
CciI TCATGA 1 cut(s) 282
Cfr13I GGNCC 5 cut(s) 353, 709, 749, 814, 1079
Cfr42I CCGCGG 1 cut(s) 233
CseI GACGC 2 cut(s) 198, 492
CviAII CATG 7 cut(s) 166, 222, 283, 492, 893, 940, 1088
DdeI CTNAG 3 cut(s) 563, 1159, 1187
DpnI GATC 4 cut(s) 94, 281, 568, 880
DpnII GATC 4 cut(s) 92, 279, 566, 878
DraIII CACNNNGTG 1 cut(s) 109
EaeI YGGCCR 1 cut(s) 224
Eco130I CCWWGG 2 cut(s) 752, 965
Eco31I GGTCTC 1 cut(s) 105
Eco47I GGWCC 2 cut(s) 353, 749
Eco57I CTGAAG 4 cut(s) 5, 356, 543, 1011
EcoNI CCTNNNNNAGG 1 cut(s) 127
EcoRI GAATTC 1 cut(s) 517
EcoRII CCWGG 5 cut(s) 349, 356, 420, 802, 1167
EcoT14I CCWWGG 2 cut(s) 752, 965
ErhI CCWWGG 2 cut(s) 752, 965
Esp3I CGTCTC 1 cut(s) 385
FaeI CATG 7 cut(s) 169, 225, 286, 495, 896, 943, 1091
FaqI GGGAC 2 cut(s) 286, 339
FatI CATG 7 cut(s) 165, 221, 282, 491, 892, 939, 1087
FblI GTMKAC 2 cut(s) 16, 481
Fnu4HI GCNGC 7 cut(s) 170, 227, 230, 347, 468, 1074, 1192
FokI GGATG 4 cut(s) 100, 200, 767, 788
Fsp4HI GCNGC 7 cut(s) 170, 227, 230, 347, 468, 1074, 1192
FspBI CTAG 3 cut(s) 305, 924, 966
GluI GCNGC 7 cut(s) 170, 227, 230, 347, 468, 1074, 1192
GsuI CTGGAG 5 cut(s) 634, 642, 968, 1041, 1088
HaeIII GGCC 4 cut(s) 226, 710, 815, 1080
HapII CCGG 2 cut(s) 106, 173
HgaI GACGC 2 cut(s) 198, 492
Hin1I GRCGYC 2 cut(s) 209, 484
Hin1II CATG 7 cut(s) 169, 225, 286, 495, 896, 943, 1091
HindIII AAGCTT 1 cut(s) 435
HinfI GANTC 1 cut(s) 362
HpaII CCGG 2 cut(s) 106, 173
HphI GGTGA 3 cut(s) 47, 95, 669
Hpy166II GTNNAC 2 cut(s) 17, 482
Hpy188I TCNGA 7 cut(s) 214, 516, 523, 566, 878, 930, 999
Hpy188III TCNNGA 9 cut(s) 10, 23, 116, 130, 283, 651, 744, 1033, 1058
Hpy8I GTNNAC 2 cut(s) 17, 482
HpyAV CCTTC 4 cut(s) 377, 470, 929, 1174
HpyCH4III ACNGT 2 cut(s) 545, 1021
HpyCH4IV ACGT 2 cut(s) 326, 394
HpyCH4V TGCA 4 cut(s) 264, 495, 763, 896
HpyF10VI GCNNNNNNNGC 6 cut(s) 175, 492, 716, 785, 1169, 1191
HpyF3I CTNAG 3 cut(s) 563, 1159, 1187
HpySE526I ACGT 2 cut(s) 326, 394
Hsp92I GRCGYC 2 cut(s) 209, 484
Hsp92II CATG 7 cut(s) 169, 225, 286, 495, 896, 943, 1091
KspI CCGCGG 1 cut(s) 233
Kzo9I GATC 4 cut(s) 92, 279, 566, 878
LmnI GCTCC 3 cut(s) 45, 175, 623
Lsp1109I GCAGC 4 cut(s) 358, 454, 1060, 1203
LweI GCATC 3 cut(s) 127, 592, 766
MaeI CTAG 3 cut(s) 305, 924, 966
MaeII ACGT 2 cut(s) 326, 394
MaeIII GTNAC 3 cut(s) 631, 675, 1015
MalI GATC 4 cut(s) 94, 281, 568, 880
MboI GATC 4 cut(s) 92, 279, 566, 878
MboII GAAGA 3 cut(s) 247, 362, 536
MluCI AATT 9 cut(s) 74, 201, 268, 517, 571, 587, 830, 844, 1036
MmeI TCCRAC 1 cut(s) 535
MnlI CCTC 8 cut(s) 48, 90, 133, 177, 199, 658, 740, 800
MseI TTAA 2 cut(s) 428, 950
MslI CAYNNNNRTG 2 cut(s) 967, 1092
MspA1I CMGCKG 2 cut(s) 232, 1045
MspI CCGG 2 cut(s) 106, 173
MspR9I CCNGG 6 cut(s) 107, 351, 358, 422, 804, 1169
Mva1269I GAATGC 2 cut(s) 765, 1172
MvaI CCWGG 5 cut(s) 351, 358, 422, 804, 1169
MvnI CGCG 1 cut(s) 232
MwoI GCNNNNNNNGC 6 cut(s) 175, 492, 716, 785, 1169, 1191
NciI CCSGG 1 cut(s) 107
NdeII GATC 4 cut(s) 92, 279, 566, 878
NlaIII CATG 7 cut(s) 169, 225, 286, 495, 896, 943, 1091
NlaIV GGNNCC 6 cut(s) 355, 583, 625, 742, 816, 963
NmuCI GTSAC 2 cut(s) 631, 675
NspI RCATGY 3 cut(s) 169, 495, 943
OliI CACNNNNGTG 1 cut(s) 967
PaeI GCATGC 1 cut(s) 943
PagI TCATGA 1 cut(s) 282
PasI CCCWGGG 1 cut(s) 357
PctI GAATGC 2 cut(s) 765, 1172
PfeI GAWTC 1 cut(s) 362
PkrI GCNGC 7 cut(s) 171, 228, 231, 348, 469, 1075, 1193
Psp6I CCWGG 5 cut(s) 349, 356, 420, 802, 1167
PspGI CCWGG 5 cut(s) 349, 356, 420, 802, 1167
PspN4I GGNNCC 6 cut(s) 355, 583, 625, 742, 816, 963
PspPI GGNCC 5 cut(s) 353, 709, 749, 814, 1079
PvuII CAGCTG 1 cut(s) 1045
RseI CAYNNNNRTG 2 cut(s) 967, 1092
SacII CCGCGG 1 cut(s) 233
SaqAI TTAA 2 cut(s) 428, 950
SatI GCNGC 7 cut(s) 170, 227, 230, 347, 468, 1074, 1192
Sau3AI GATC 4 cut(s) 92, 279, 566, 878
Sau96I GGNCC 5 cut(s) 353, 709, 749, 814, 1079
ScrFI CCNGG 6 cut(s) 107, 351, 358, 422, 804, 1169
SfaNI GCATC 3 cut(s) 127, 592, 766
Sfr303I CCGCGG 1 cut(s) 233
SgrBI CCGCGG 1 cut(s) 233
SinI GGWCC 2 cut(s) 353, 749
SmiMI CAYNNNNRTG 2 cut(s) 967, 1092
SphI GCATGC 1 cut(s) 943
Sse9I AATT 9 cut(s) 74, 201, 268, 517, 571, 587, 830, 844, 1036
SsiI CCGC 5 cut(s) 170, 227, 230, 232, 388
SspMI CTAG 3 cut(s) 305, 924, 966
StyD4I CCNGG 6 cut(s) 105, 349, 356, 420, 802, 1167
StyI CCWWGG 2 cut(s) 752, 965
TaaI ACNGT 2 cut(s) 545, 1021
TaiI ACGT 2 cut(s) 329, 397
TaqI TCGA 2 cut(s) 53, 95
TasI AATT 9 cut(s) 74, 201, 268, 517, 571, 587, 830, 844, 1036
TauI GCSGC 3 cut(s) 172, 229, 232
TfiI GAWTC 1 cut(s) 362
Tru1I TTAA 2 cut(s) 428, 950
Tru9I TTAA 2 cut(s) 428, 950
TscAI CASTG 4 cut(s) 380, 550, 770, 992
TseFI GTSAC 2 cut(s) 631, 675
TseI GCWGC 4 cut(s) 346, 467, 1073, 1191
Tsp45I GTSAC 2 cut(s) 631, 675
TspDTI ATGAA 5 cut(s) 44, 299, 680, 862, 948
TspGWI ACGGA 1 cut(s) 1080
TspRI CASTG 4 cut(s) 380, 550, 770, 992
VpaK11BI GGWCC 2 cut(s) 353, 749
XagI CCTNNNNNAGG 1 cut(s) 127
XapI RAATTY 6 cut(s) 74, 517, 571, 587, 830, 1036
XceI RCATGY 3 cut(s) 169, 495, 943
XmaJI CCTAGG 1 cut(s) 965
XmiI GTMKAC 2 cut(s) 16, 481
XspI CTAG 3 cut(s) 305, 924, 966
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.