Prupe.8G253000_v2.0.a1

Tubby-like F-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0001645
Physical Location & Seq
Reverse (-)
21670736 .. 21673787
3052 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G253000.1

Sequence Viewer

Length: 1206 bp
ATGTCTTTGAAGAGTATATTTCAAGATATGAAAGGCGAGTTTGGGAGCATTTCTAGGAAAAGCTTTGATGTGAAATTTGGTATAAGATCAAGGTCTCAACGGGTGGTTCAGGATACCACCTCATTTCGAGTTGATGCTTTGAAGCAGAGTTGTTGGGCTAACATGCCACCGGAGCTTTTGAGGGATGTGTTGATGAGAATAGAGGCATCTGATGATACATGGCCTCCGCGGAAAAATGTAGTTTCTTGTGCTGGTGTTTGCAGGAATTGGAGAGAAATCATGAAAGAGATTGTGAAATCCCCAGAAGTTTCGGGCAAGCTGACATTCCCAATCTCTTTGAAGCAGCCTGGTCAAAGGGGATCCCTTCTTCAGTGTTATATAAAACGGAGTCGTAGCAACCAGACATATTATCTTTTTCTGGGTTTAAATCAAGCCTCAACTGATGATGGGAAGTTCCTTCTTGCTGCAAAAAAGTGTAGACGCCCAACCTGCACAGACTACATCATCTCTGTAAATGCTGAGGATGTGTCAAAAGGGAACAGTGGCTTTGTCGGAAAGCTCAGATCAAATTTTCTGGGCACTAAGTTCACAATCTATGATGCGCAACCTACTAATACTGGAGCCAAAGTCACTAAATGTCGTTCAACCAGGCTTGTAAATATGAAACAAGTCTCCCCCAGGGTTCCTGCTGGCAACTACCCTGTGGCCCACATTGCTTATGAGTTGAACGTCTTGGGTTCCAGAGGTCCGAGGAGAATGCAGTGTGTAATGGATGCCATCCCTGCCCGTGCTGTTGAGCCAGGAGGCGTGGCACCCACACAGACTGAATTTCTGCATAGCAACCCGGATAGTTTTCCATCCCTCCCTTTTTTCAGATCAACATCAACCCGGATGGAGAATTTCGAGTCTGGTGAGAATGGAGGTATGCTGGTATTGAGGAACAAGGCGCCTAGGTGGCATGAACAACTCCAGTGCTGGTGTCTCAACTTCAATGGACGAGTAACAGTTGCTTCCGTCAAGAATTTTCAGCTTGTTGCTTCCCCAGAGAACGGAGTTGCTGCTGGCCAGGAGCATGAGAATGTCATCCTCCAGTTTGGAAAAGTGGGCAAGGATGTATTCACCATGGATTATCAGTATCCAATCTCAGCCTTTCAGGCATTTGCAATTTGTCTGAGCAGCTTTGACACCAAGATTGCTTGTGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005543 GO:0008289 GO:0035091 GO:0043167 GO:0043168
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box Tub

Protein Analysis

402

Amino Acids

44.8

Weight (kDa)

9.48

Isoelectric Point (pI)

48.94

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 603
Acc36I ACCTGC 1 cut(s) 497
AccB1I GGYRCC 2 cut(s) 811, 946
AccI GTMKAC 1 cut(s) 478
AccII CGCG 1 cut(s) 229
AciI CCGC 2 cut(s) 227, 229
AclWI GGATC 2 cut(s) 354, 367
AcoI YGGCCR 1 cut(s) 1063
AcsI RAATTY 5 cut(s) 74, 568, 827, 898, 1021
AcuI CTGAAG 1 cut(s) 353
AcyI GRCGYC 2 cut(s) 481, 947
AfiI CCNNNNNNNGG 1 cut(s) 1049
AgsI TTSAA 7 cut(s) 10, 23, 142, 340, 645, 727, 991
AjnI CCWGG 5 cut(s) 346, 647, 677, 799, 1065
AluBI AGCT 6 cut(s) 63, 175, 319, 559, 1030, 1179
AluI AGCT 6 cut(s) 63, 175, 319, 559, 1030, 1179
Alw26I GTCTC 3 cut(s) 99, 676, 986
AlwI GGATC 2 cut(s) 354, 367
AoxI GGCC 3 cut(s) 221, 705, 1063
ApeKI GCWGC 4 cut(s) 343, 464, 1058, 1176
ApoI RAATTY 5 cut(s) 74, 568, 827, 898, 1021
AspA2I CCTAGG 1 cut(s) 950
AspLEI GCGC 2 cut(s) 604, 949
AspS9I GGNCC 2 cut(s) 706, 746
AsuC2I CCSGG 2 cut(s) 845, 889
AsuHPI GGTGA 2 cut(s) 923, 1111
AvaII GGWCC 1 cut(s) 746
AvrII CCTAGG 1 cut(s) 950
BaeGI GKGCMC 1 cut(s) 581
BalI TGGCCA 1 cut(s) 1065
BamHI GGATCC 1 cut(s) 359
BanI GGYRCC 2 cut(s) 811, 946
BbvCI CCTCAGC 1 cut(s) 519
BbvI GCAGC 4 cut(s) 355, 451, 1045, 1188
BccI CCATC 4 cut(s) 440, 785, 865, 886
BcgI CGANNNNNNTGC 2 cut(s) 739, 773
BciT130I CCWGG 5 cut(s) 348, 649, 679, 801, 1067
BciVI GTATCC 2 cut(s) 106, 1146
BcnI CCSGG 2 cut(s) 845, 889
BcoDI GTCTC 3 cut(s) 99, 676, 986
BfaI CTAG 2 cut(s) 54, 951
BfoI RGCGCY 1 cut(s) 950
BfuAI ACCTGC 1 cut(s) 497
BfuI GTATCC 2 cut(s) 106, 1146
BglI GCCNNNNNGGC 2 cut(s) 955, 1154
BisI GCNGC 4 cut(s) 344, 465, 1059, 1177
BlnI CCTAGG 1 cut(s) 950
BlsI GCNGC 4 cut(s) 345, 466, 1060, 1178
Bme1390I CCNGG 7 cut(s) 348, 649, 679, 801, 845, 889, 1067
Bme18I GGWCC 1 cut(s) 746
BmgT120I GGNCC 2 cut(s) 706, 746
BmiI GGNNCC 6 cut(s) 361, 622, 684, 739, 813, 948
BmrFI CCNGG 7 cut(s) 348, 649, 679, 801, 845, 889, 1067
BmsI GCATC 4 cut(s) 124, 215, 589, 763
BpmI CTGGAG 3 cut(s) 639, 953, 1073
Bpu10I CCTNAGC 1 cut(s) 519
BpuMI CCSGG 2 cut(s) 845, 889
BsaBI GATNNNNATC 1 cut(s) 880
BsaHI GRCGYC 2 cut(s) 481, 947
BsaI GGTCTC 1 cut(s) 99
BsaJI CCNNGG 6 cut(s) 227, 677, 678, 749, 950, 1122
BsaWI WCCGGW 1 cut(s) 169
BsaXI ACNNNNNCTCC 4 cut(s) 208, 238, 612, 642
Bsc4I CCNNNNNNNGG 1 cut(s) 1049
Bse1I ACTGG 3 cut(s) 622, 970, 1090
Bse3DI GCAATG 1 cut(s) 711
Bse8I GATNNNNATC 1 cut(s) 880
BseBI CCWGG 5 cut(s) 348, 649, 679, 801, 1067
BseDI CCNNGG 6 cut(s) 227, 677, 678, 749, 950, 1122
BseGI GGATG 8 cut(s) 190, 529, 777, 778, 857, 897, 1083, 1117
BseJI GATNNNNATC 1 cut(s) 880
BseLI CCNNNNNNNGG 1 cut(s) 1049
BseMI GCAATG 1 cut(s) 711
BseMII CTCAG 4 cut(s) 510, 574, 1158, 1163
BseNI ACTGG 3 cut(s) 622, 970, 1090
BseRI GAGGAG 1 cut(s) 766
BseSI GKGCMC 1 cut(s) 581
BseXI GCAGC 4 cut(s) 355, 451, 1045, 1188
BsgI GTGCAG 1 cut(s) 475
Bsh1236I CGCG 1 cut(s) 229
BshFI GGCC 3 cut(s) 223, 707, 1065
BshNI GGYRCC 2 cut(s) 811, 946
BsiSI CCGG 3 cut(s) 170, 845, 889
BslI CCNNNNNNNGG 1 cut(s) 1049
BsmAI GTCTC 3 cut(s) 99, 676, 986
BsmI GAATGC 1 cut(s) 762
BsnI GGCC 3 cut(s) 223, 707, 1065
Bso31I GGTCTC 1 cut(s) 99
Bsp1286I GDGCHC 1 cut(s) 581
Bsp143I GATC 4 cut(s) 86, 359, 563, 875
Bsp19I CCATGG 1 cut(s) 1122
BspACI CCGC 2 cut(s) 227, 229
BspANI GGCC 3 cut(s) 223, 707, 1065
BspCNI CTCAG 4 cut(s) 511, 573, 1157, 1164
BspFNI CGCG 1 cut(s) 229
BspHI TCATGA 1 cut(s) 279
BspLI GGNNCC 6 cut(s) 361, 622, 684, 739, 813, 948
BspMI ACCTGC 1 cut(s) 497
BspPI GGATC 2 cut(s) 354, 367
BspT107I GGYRCC 2 cut(s) 811, 946
BspTNI GGTCTC 1 cut(s) 99
BsrDI GCAATG 1 cut(s) 711
BsrI ACTGG 3 cut(s) 622, 970, 1090
BssECI CCNNGG 6 cut(s) 227, 677, 678, 749, 950, 1122
BssMI GATC 4 cut(s) 86, 359, 563, 875
BssNI GRCGYC 2 cut(s) 481, 947
BssT1I CCWWGG 2 cut(s) 950, 1122
Bst2UI CCWGG 5 cut(s) 348, 649, 679, 801, 1067
Bst4CI ACNGT 2 cut(s) 542, 1006
Bst6I CTCTTC 1 cut(s) 5
BstACI GRCGYC 2 cut(s) 481, 947
BstC8I GCNNGC 3 cut(s) 317, 691, 1063
BstDEI CTNAG 5 cut(s) 519, 560, 582, 1144, 1172
BstDSI CCRYGG 2 cut(s) 227, 1122
BstF5I GGATG 8 cut(s) 190, 529, 777, 778, 857, 897, 1083, 1117
BstFNI CGCG 1 cut(s) 229
BstH2I RGCGCY 1 cut(s) 950
BstHHI GCGC 2 cut(s) 604, 949
BstKTI GATC 4 cut(s) 89, 362, 566, 878
BstMAI GTCTC 3 cut(s) 99, 676, 986
BstMBI GATC 4 cut(s) 86, 359, 563, 875
BstMWI GCNNNNNNNGC 6 cut(s) 172, 489, 713, 782, 955, 1154
BstNI CCWGG 5 cut(s) 348, 649, 679, 801, 1067
BstNSI RCATGY 1 cut(s) 166
BstSCI CCNGG 7 cut(s) 346, 647, 677, 799, 843, 887, 1065
BstSLI GKGCMC 1 cut(s) 581
BstUI CGCG 1 cut(s) 229
BstV1I GCAGC 4 cut(s) 355, 451, 1045, 1188
BstX2I RGATCY 1 cut(s) 359
BstYI RGATCY 1 cut(s) 359
BsuI GTATCC 2 cut(s) 106, 1146
BsuRI GGCC 3 cut(s) 223, 707, 1065
BtgI CCRYGG 2 cut(s) 227, 1122
BtsCI GGATG 8 cut(s) 190, 529, 777, 778, 857, 897, 1083, 1117
BtsI GCAGTG 1 cut(s) 767
BtsIMutI CAGTG 4 cut(s) 377, 547, 767, 977
BveI ACCTGC 1 cut(s) 497
Cac8I GCNNGC 3 cut(s) 317, 691, 1063
CciI TCATGA 1 cut(s) 279
CfoI GCGC 2 cut(s) 604, 949
Cfr13I GGNCC 2 cut(s) 706, 746
Cfr42I CCGCGG 1 cut(s) 230
CseI GACGC 1 cut(s) 489
CviAII CATG 6 cut(s) 163, 219, 280, 959, 1073, 1123
DdeI CTNAG 5 cut(s) 519, 560, 582, 1144, 1172
DinI GGCGCC 1 cut(s) 948
DpnI GATC 4 cut(s) 88, 361, 565, 877
DpnII GATC 4 cut(s) 86, 359, 563, 875
DraI TTTAAA 1 cut(s) 426
EaeI YGGCCR 1 cut(s) 1063
Eam1104I CTCTTC 1 cut(s) 5
EarI CTCTTC 1 cut(s) 5
Eco130I CCWWGG 2 cut(s) 950, 1122
Eco31I GGTCTC 1 cut(s) 99
Eco47I GGWCC 1 cut(s) 746
Eco57I CTGAAG 1 cut(s) 353
EcoRII CCWGG 5 cut(s) 346, 647, 677, 799, 1065
EcoT14I CCWWGG 2 cut(s) 950, 1122
EgeI GGCGCC 1 cut(s) 948
EheI GGCGCC 1 cut(s) 948
ErhI CCWWGG 2 cut(s) 950, 1122
FaeI CATG 6 cut(s) 166, 222, 283, 962, 1076, 1126
FatI CATG 6 cut(s) 162, 218, 279, 958, 1072, 1122
FblI GTMKAC 1 cut(s) 478
Fnu4HI GCNGC 4 cut(s) 344, 465, 1059, 1177
FokI GGATG 8 cut(s) 197, 536, 764, 785, 844, 904, 1070, 1124
Fsp4HI GCNGC 4 cut(s) 344, 465, 1059, 1177
FspBI CTAG 2 cut(s) 54, 951
FspI TGCGCA 1 cut(s) 603
GlaI GCGC 2 cut(s) 603, 948
GluI GCNGC 4 cut(s) 344, 465, 1059, 1177
GsuI CTGGAG 3 cut(s) 639, 953, 1073
HaeII RGCGCY 1 cut(s) 950
HaeIII GGCC 3 cut(s) 223, 707, 1065
HapII CCGG 3 cut(s) 170, 845, 889
HgaI GACGC 1 cut(s) 489
HhaI GCGC 2 cut(s) 604, 949
Hin1I GRCGYC 2 cut(s) 481, 947
Hin1II CATG 6 cut(s) 166, 222, 283, 962, 1076, 1126
Hin6I GCGC 2 cut(s) 602, 947
HinP1I GCGC 2 cut(s) 602, 947
HindIII AAGCTT 1 cut(s) 61
HinfI GANTC 2 cut(s) 388, 905
HpaII CCGG 3 cut(s) 170, 845, 889
HphI GGTGA 2 cut(s) 923, 1111
Hpy166II GTNNAC 2 cut(s) 479, 588
Hpy188I TCNGA 6 cut(s) 211, 554, 563, 750, 875, 1173
Hpy188III TCNNGA 5 cut(s) 23, 110, 280, 741, 1018
Hpy8I GTNNAC 2 cut(s) 479, 588
HpyAV CCTTC 2 cut(s) 374, 467
HpyCH4III ACNGT 2 cut(s) 542, 1006
HpyCH4IV ACGT 1 cut(s) 729
HpyCH4V TGCA 6 cut(s) 261, 467, 492, 760, 835, 1163
HpyF10VI GCNNNNNNNGC 6 cut(s) 172, 489, 713, 782, 955, 1154
HpyF3I CTNAG 5 cut(s) 519, 560, 582, 1144, 1172
HpySE526I ACGT 1 cut(s) 729
Hsp92I GRCGYC 2 cut(s) 481, 947
Hsp92II CATG 6 cut(s) 166, 222, 283, 962, 1076, 1126
HspAI GCGC 2 cut(s) 602, 947
KasI GGCGCC 1 cut(s) 946
KspI CCGCGG 1 cut(s) 230
Kzo9I GATC 4 cut(s) 86, 359, 563, 875
LmnI GCTCC 4 cut(s) 45, 172, 620, 1069
Lsp1109I GCAGC 4 cut(s) 355, 451, 1045, 1188
LweI GCATC 4 cut(s) 124, 215, 589, 763
MaeI CTAG 2 cut(s) 54, 951
MaeII ACGT 1 cut(s) 729
MaeIII GTNAC 2 cut(s) 628, 1000
MalI GATC 4 cut(s) 88, 361, 565, 877
MboI GATC 4 cut(s) 86, 359, 563, 875
MboII GAAGA 2 cut(s) 22, 359
MflI RGATCY 1 cut(s) 359
MhlI GDGCHC 1 cut(s) 581
MlsI TGGCCA 1 cut(s) 1065
MluCI AATT 7 cut(s) 74, 265, 568, 827, 898, 1021, 1164
MluNI TGGCCA 1 cut(s) 1065
Mly113I GGCGCC 1 cut(s) 947
MlyI GAGTC 2 cut(s) 397, 914
MmeI TCCRAC 1 cut(s) 532
Mox20I TGGCCA 1 cut(s) 1065
MscI TGGCCA 1 cut(s) 1065
MseI TTAA 1 cut(s) 425
MslI CAYNNNNRTG 1 cut(s) 1077
Msp20I TGGCCA 1 cut(s) 1065
MspA1I CMGCKG 1 cut(s) 229
MspI CCGG 3 cut(s) 170, 845, 889
MspR9I CCNGG 7 cut(s) 348, 649, 679, 801, 845, 889, 1067
Mva1269I GAATGC 1 cut(s) 762
MvaI CCWGG 5 cut(s) 348, 649, 679, 801, 1067
MvnI CGCG 1 cut(s) 229
MwoI GCNNNNNNNGC 6 cut(s) 172, 489, 713, 782, 955, 1154
NarI GGCGCC 1 cut(s) 947
NciI CCSGG 2 cut(s) 845, 889
NcoI CCATGG 1 cut(s) 1122
NdeII GATC 4 cut(s) 86, 359, 563, 875
NlaIII CATG 6 cut(s) 166, 222, 283, 962, 1076, 1126
NlaIV GGNNCC 6 cut(s) 361, 622, 684, 739, 813, 948
NmuCI GTSAC 1 cut(s) 628
NsbI TGCGCA 1 cut(s) 603
NspI RCATGY 1 cut(s) 166
PagI TCATGA 1 cut(s) 279
PasI CCCWGGG 1 cut(s) 678
PctI GAATGC 1 cut(s) 762
PkrI GCNGC 4 cut(s) 345, 466, 1060, 1178
PleI GAGTC 2 cut(s) 396, 913
PluTI GGCGCC 1 cut(s) 950
PpsI GAGTC 2 cut(s) 396, 913
Psp6I CCWGG 5 cut(s) 346, 647, 677, 799, 1065
PspGI CCWGG 5 cut(s) 346, 647, 677, 799, 1065
PspN4I GGNNCC 6 cut(s) 361, 622, 684, 739, 813, 948
PspPI GGNCC 2 cut(s) 706, 746
PsuI RGATCY 1 cut(s) 359
RseI CAYNNNNRTG 1 cut(s) 1077
SacII CCGCGG 1 cut(s) 230
SaqAI TTAA 1 cut(s) 425
SatI GCNGC 4 cut(s) 344, 465, 1059, 1177
Sau3AI GATC 4 cut(s) 86, 359, 563, 875
Sau96I GGNCC 2 cut(s) 706, 746
SchI GAGTC 2 cut(s) 397, 914
ScrFI CCNGG 7 cut(s) 348, 649, 679, 801, 845, 889, 1067
SduI GDGCHC 1 cut(s) 581
SfaNI GCATC 4 cut(s) 124, 215, 589, 763
SfoI GGCGCC 1 cut(s) 948
Sfr303I CCGCGG 1 cut(s) 230
SgrBI CCGCGG 1 cut(s) 230
SinI GGWCC 1 cut(s) 746
SmiMI CAYNNNNRTG 1 cut(s) 1077
Sse9I AATT 7 cut(s) 74, 265, 568, 827, 898, 1021, 1164
SsiI CCGC 2 cut(s) 227, 229
SspDI GGCGCC 1 cut(s) 946
SspMI CTAG 2 cut(s) 54, 951
StyD4I CCNGG 7 cut(s) 346, 647, 677, 799, 843, 887, 1065
StyI CCWWGG 2 cut(s) 950, 1122
TaaI ACNGT 2 cut(s) 542, 1006
TaiI ACGT 1 cut(s) 732
TaqI TCGA 2 cut(s) 127, 903
TasI AATT 7 cut(s) 74, 265, 568, 827, 898, 1021, 1164
Tru1I TTAA 1 cut(s) 425
Tru9I TTAA 1 cut(s) 425
TscAI CASTG 4 cut(s) 377, 547, 767, 977
TseFI GTSAC 1 cut(s) 628
TseI GCWGC 4 cut(s) 343, 464, 1058, 1176
Tsp45I GTSAC 1 cut(s) 628
TspDTI ATGAA 4 cut(s) 44, 296, 677, 975
TspGWI ACGGA 3 cut(s) 400, 1003, 1065
TspRI CASTG 4 cut(s) 377, 547, 767, 977
VpaK11BI GGWCC 1 cut(s) 746
XapI RAATTY 5 cut(s) 74, 568, 827, 898, 1021
XceI RCATGY 1 cut(s) 166
XmaJI CCTAGG 1 cut(s) 950
XmiI GTMKAC 1 cut(s) 478
XspI CTAG 2 cut(s) 54, 951
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.