Rmu_sc0000956.1_g000003

Tubby-like F-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000956.1
OG0001645
Physical Location & Seq
Forward (+)
6062 .. 7258
1197 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000956.1_g000003.1.cds

Sequence Viewer

Length: 1197 bp
atgtctttcaagagtatacttcaggataagaaaggcgagtttggaagcagttggaggaaacgtcttaatctgaaatttagttatgggatgatatctaggtctcaccgggtggttcaggacacctcgttcaggattgatgctttgaagcagagttgttgggctaacgtgccgccggagcttttgagggaggtgttgatgagaattgaggcgtctgatgatacatggcctctgcggaaaaatgtggtcgcttgtgctggtgtgtgcaacaattggagggagatgatgaaagagattgtgaagtccccagaagtttcgggcaagctgacattcccaatctccttgaagcagcctggtcccagggacttccttctccaatgttatataaagcgtagcaaccaggcatattatcttttcctgggcttaaatcaagcttcaactgatgatgggaagttccttcttgctgcgagaaagcgtagacgcctaacatgcacagattacatcatctctctgaattctgaaaacgtgtcaaaagggaacagtgcctttgttggaaagctcagatcaaattttctgggtaccaaatttacaatctatgatgcgcaaccaagtaatactggagccaatgtcactacatgtcattccaccatgaaacaagtgtcacccagagttcctgctggcgactatcctgtgggccacattgcgtatgagttgaacgtcttgggttccagaggttcaaggagaatgaagtgcgtactggatgccatccctgctcgtgccgttgacccaggaggtgcccctacgcagactgaatttgtcaatagcaattcggatagtattccatctctccctctcttcggatcaaaatcaggtcccgcggagagtgtccaatctggacaacaatctcagaatgaagggatgctggtgttaaggagcaaggcacctaggtggctcgaacaactccagcggtggtgcttgaacttcaatcgacgagtaaccgaagcttcagtcaagaattttcagctggttgcttctccagagaacggagttgctgctgacccagagcatgagaatgtcattctcgagtgtggaaaagtaggcaaagacgtattcacaatggattaccagtatccaatctcagccttccaggcatttgctatttgccttagcagcattgactccaagattgcttgtgaatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005543 GO:0008289 GO:0035091 GO:0043167 GO:0043168
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box Tub

Protein Analysis

398

Amino Acids

44.32

Weight (kDa)

9.29

Isoelectric Point (pI)

55.73

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 600
Acc65I GGTACC 1 cut(s) 575
AccB1I GGYRCC 3 cut(s) 575, 791, 937
AccI GTMKAC 2 cut(s) 16, 475
AccII CGCG 1 cut(s) 875
AciI CCGC 5 cut(s) 170, 232, 873, 875, 964
AclWI GGATC 1 cut(s) 865
AcsI RAATTY 6 cut(s) 74, 511, 565, 581, 809, 1012
AcuI CTGAAG 2 cut(s) 5, 987
AcyI GRCGYC 2 cut(s) 209, 478
AdeI CACNNNGTG 1 cut(s) 109
AfaI GTAC 2 cut(s) 577, 753
AfiI CCNNNNNNNGG 3 cut(s) 129, 854, 1040
AflIII ACRYGT 2 cut(s) 522, 632
AgsI TTSAA 8 cut(s) 10, 145, 343, 435, 712, 735, 976, 982
AjnI CCWGG 6 cut(s) 349, 356, 396, 414, 784, 1143
AleI CACNNNNGTG 1 cut(s) 943
AloI GAACNNNNNNTCC 2 cut(s) 110, 142
AluBI AGCT 6 cut(s) 178, 322, 431, 556, 1001, 1021
AluI AGCT 6 cut(s) 178, 322, 431, 556, 1001, 1021
Alw26I GTCTC 1 cut(s) 105
AlwI GGATC 1 cut(s) 865
Ama87I CYCGRG 1 cut(s) 1079
AoxI GGCC 2 cut(s) 224, 691
ApeKI GCWGC 4 cut(s) 346, 461, 1049, 1167
ApoI RAATTY 6 cut(s) 74, 511, 565, 581, 809, 1012
ArsI GACNNNNNNTTYG 2 cut(s) 990, 1022
Asp718I GGTACC 1 cut(s) 575
AspA2I CCTAGG 1 cut(s) 941
AspLEI GCGC 1 cut(s) 601
AspS9I GGNCC 3 cut(s) 353, 691, 869
AsuC2I CCSGG 1 cut(s) 107
AsuHPI GGTGA 2 cut(s) 95, 651
AvaI CYCGRG 1 cut(s) 1079
AvaII GGWCC 2 cut(s) 353, 869
AvrII CCTAGG 1 cut(s) 941
BaeGI GKGCMC 1 cut(s) 796
BanI GGYRCC 3 cut(s) 575, 791, 937
BauI CACGAG 1 cut(s) 771
BbvI GCAGC 4 cut(s) 358, 448, 1036, 1179
BccI CCATC 3 cut(s) 437, 770, 847
BceAI ACGGC 1 cut(s) 761
BciT130I CCWGG 6 cut(s) 351, 358, 398, 416, 786, 1145
BciVI GTATCC 1 cut(s) 1137
BcnI CCSGG 1 cut(s) 107
BcoDI GTCTC 1 cut(s) 105
BfaI CTAG 2 cut(s) 96, 942
BfuI GTATCC 1 cut(s) 1137
BglI GCCNNNNNGGC 1 cut(s) 1145
BisI GCNGC 5 cut(s) 170, 347, 462, 1050, 1168
BlnI CCTAGG 1 cut(s) 941
BlsI GCNGC 5 cut(s) 171, 348, 463, 1051, 1169
Bme1390I CCNGG 7 cut(s) 107, 351, 358, 398, 416, 786, 1145
Bme18I GGWCC 2 cut(s) 353, 869
BmeT110I CYCGRG 1 cut(s) 1079
BmgT120I GGNCC 3 cut(s) 353, 691, 869
BmiI GGNNCC 7 cut(s) 355, 577, 619, 724, 793, 871, 939
BmrFI CCNGG 7 cut(s) 107, 351, 358, 398, 416, 786, 1145
BmsI GCATC 4 cut(s) 127, 586, 748, 906
BpmI CTGGAG 3 cut(s) 636, 944, 1017
Bpu10I CCTNAGC 1 cut(s) 1163
BpuMI CCSGG 1 cut(s) 107
BsaBI GATNNNNATC 1 cut(s) 862
BsaHI GRCGYC 2 cut(s) 209, 478
BsaI GGTCTC 1 cut(s) 105
BsaJI CCNNGG 6 cut(s) 356, 357, 415, 784, 873, 941
BsaXI ACNNNNNCTCC 4 cut(s) 46, 76, 609, 639
Bsc4I CCNNNNNNNGG 3 cut(s) 129, 854, 1040
Bse1I ACTGG 3 cut(s) 619, 759, 1123
Bse3DI GCAATG 1 cut(s) 696
Bse8I GATNNNNATC 1 cut(s) 862
BseBI CCWGG 6 cut(s) 351, 358, 398, 416, 786, 1145
BseDI CCNNGG 6 cut(s) 356, 357, 415, 784, 873, 941
BseGI GGATG 4 cut(s) 93, 762, 763, 921
BseJI GATNNNNATC 1 cut(s) 862
BseLI CCNNNNNNNGG 3 cut(s) 129, 854, 1040
BseMI GCAATG 1 cut(s) 696
BseMII CTCAG 3 cut(s) 571, 917, 1149
BseNI ACTGG 3 cut(s) 619, 759, 1123
BseSI GKGCMC 1 cut(s) 796
BseXI GCAGC 4 cut(s) 358, 448, 1036, 1179
Bsh1236I CGCG 1 cut(s) 875
BshFI GGCC 2 cut(s) 226, 693
BshNI GGYRCC 3 cut(s) 575, 791, 937
BsiHKCI CYCGRG 1 cut(s) 1079
BsiSI CCGG 2 cut(s) 106, 173
BslFI GGGAC 4 cut(s) 286, 339, 374, 855
BslI CCNNNNNNNGG 3 cut(s) 129, 854, 1040
BsmAI GTCTC 1 cut(s) 105
BsmFI GGGAC 4 cut(s) 286, 339, 374, 855
BsnI GGCC 2 cut(s) 226, 693
Bso31I GGTCTC 1 cut(s) 105
BsoBI CYCGRG 1 cut(s) 1079
Bsp1286I GDGCHC 1 cut(s) 796
Bsp143I GATC 2 cut(s) 560, 857
BspACI CCGC 5 cut(s) 170, 232, 873, 875, 964
BspANI GGCC 2 cut(s) 226, 693
BspCNI CTCAG 3 cut(s) 570, 916, 1148
BspFNI CGCG 1 cut(s) 875
BspLI GGNNCC 7 cut(s) 355, 577, 619, 724, 793, 871, 939
BspPI GGATC 1 cut(s) 865
BspT107I GGYRCC 3 cut(s) 575, 791, 937
BspTNI GGTCTC 1 cut(s) 105
BsrDI GCAATG 1 cut(s) 696
BsrI ACTGG 3 cut(s) 619, 759, 1123
BssECI CCNNGG 6 cut(s) 356, 357, 415, 784, 873, 941
BssMI GATC 2 cut(s) 560, 857
BssNAI GTATAC 1 cut(s) 17
BssNI GRCGYC 2 cut(s) 209, 478
BssSI CACGAG 1 cut(s) 771
BssT1I CCWWGG 1 cut(s) 941
Bst1107I GTATAC 1 cut(s) 17
Bst2BI CACGAG 1 cut(s) 771
Bst2UI CCWGG 6 cut(s) 351, 358, 398, 416, 786, 1145
Bst4CI ACNGT 1 cut(s) 539
Bst6I CTCTTC 1 cut(s) 857
BstACI GRCGYC 2 cut(s) 209, 478
BstC8I GCNNGC 2 cut(s) 320, 676
BstDEI CTNAG 4 cut(s) 557, 903, 1135, 1163
BstDSI CCRYGG 1 cut(s) 873
BstENI CCTNNNNNAGG 1 cut(s) 127
BstF5I GGATG 4 cut(s) 93, 762, 763, 921
BstFNI CGCG 1 cut(s) 875
BstHHI GCGC 1 cut(s) 601
BstKTI GATC 2 cut(s) 563, 860
BstMAI GTCTC 1 cut(s) 105
BstMBI GATC 2 cut(s) 560, 857
BstMWI GCNNNNNNNGC 5 cut(s) 175, 486, 767, 1145, 1167
BstNI CCWGG 6 cut(s) 351, 358, 398, 416, 786, 1145
BstNSI RCATGY 2 cut(s) 489, 636
BstSCI CCNGG 7 cut(s) 105, 349, 356, 396, 414, 784, 1143
BstSLI GKGCMC 1 cut(s) 796
BstUI CGCG 1 cut(s) 875
BstV1I GCAGC 4 cut(s) 358, 448, 1036, 1179
BstZ17I GTATAC 1 cut(s) 17
BsuI GTATCC 1 cut(s) 1137
BsuRI GGCC 2 cut(s) 226, 693
BtgI CCRYGG 1 cut(s) 873
BtsCI GGATG 4 cut(s) 93, 762, 763, 921
BtsIMutI CAGTG 1 cut(s) 544
Cac8I GCNNGC 2 cut(s) 320, 676
CfoI GCGC 1 cut(s) 601
Cfr13I GGNCC 3 cut(s) 353, 691, 869
Cfr42I CCGCGG 1 cut(s) 876
CseI GACGC 2 cut(s) 198, 486
Csp6I GTAC 2 cut(s) 576, 752
CviAII CATG 5 cut(s) 222, 486, 633, 646, 1064
CviQI GTAC 2 cut(s) 576, 752
DdeI CTNAG 4 cut(s) 557, 903, 1135, 1163
DpnI GATC 2 cut(s) 562, 859
DpnII GATC 2 cut(s) 560, 857
DraIII CACNNNGTG 1 cut(s) 109
Eam1104I CTCTTC 1 cut(s) 857
EarI CTCTTC 1 cut(s) 857
Eco130I CCWWGG 1 cut(s) 941
Eco31I GGTCTC 1 cut(s) 105
Eco32I GATATC 1 cut(s) 93
Eco47I GGWCC 2 cut(s) 353, 869
Eco57I CTGAAG 2 cut(s) 5, 987
Eco88I CYCGRG 1 cut(s) 1079
EcoNI CCTNNNNNAGG 1 cut(s) 127
EcoO109I RGGNCCY 1 cut(s) 869
EcoRI GAATTC 1 cut(s) 511
EcoRII CCWGG 6 cut(s) 349, 356, 396, 414, 784, 1143
EcoRV GATATC 1 cut(s) 93
EcoT14I CCWWGG 1 cut(s) 941
ErhI CCWWGG 1 cut(s) 941
FaeI CATG 5 cut(s) 225, 489, 636, 649, 1067
FaqI GGGAC 4 cut(s) 286, 339, 374, 855
FatI CATG 5 cut(s) 221, 485, 632, 645, 1063
FauI CCCGC 1 cut(s) 880
FblI GTMKAC 2 cut(s) 16, 475
Fnu4HI GCNGC 5 cut(s) 170, 347, 462, 1050, 1168
FokI GGATG 4 cut(s) 100, 749, 770, 928
Fsp4HI GCNGC 5 cut(s) 170, 347, 462, 1050, 1168
FspBI CTAG 2 cut(s) 96, 942
FspI TGCGCA 1 cut(s) 600
GlaI GCGC 1 cut(s) 600
GluI GCNGC 5 cut(s) 170, 347, 462, 1050, 1168
GsuI CTGGAG 3 cut(s) 636, 944, 1017
HaeIII GGCC 2 cut(s) 226, 693
HapII CCGG 2 cut(s) 106, 173
HgaI GACGC 2 cut(s) 198, 486
HhaI GCGC 1 cut(s) 601
Hin1I GRCGYC 2 cut(s) 209, 478
Hin1II CATG 5 cut(s) 225, 489, 636, 649, 1067
Hin6I GCGC 1 cut(s) 599
HinP1I GCGC 1 cut(s) 599
HincII GTYRAC 1 cut(s) 781
HindII GTYRAC 1 cut(s) 781
HindIII AAGCTT 2 cut(s) 429, 999
HinfI GANTC 1 cut(s) 1175
HpaII CCGG 2 cut(s) 106, 173
HphI GGTGA 2 cut(s) 95, 651
Hpy166II GTNNAC 3 cut(s) 17, 476, 781
Hpy188I TCNGA 8 cut(s) 72, 214, 510, 517, 560, 829, 857, 906
Hpy188III TCNNGA 9 cut(s) 10, 23, 116, 130, 726, 891, 1009, 1034, 1079
Hpy8I GTNNAC 3 cut(s) 17, 476, 781
Hpy99I CGWCG 1 cut(s) 990
HpyAV CCTTC 4 cut(s) 377, 464, 905, 1150
HpyCH4III ACNGT 1 cut(s) 539
HpyCH4IV ACGT 5 cut(s) 61, 165, 522, 714, 1104
HpyCH4V TGCA 2 cut(s) 264, 489
HpyF10VI GCNNNNNNNGC 5 cut(s) 175, 486, 767, 1145, 1167
HpyF3I CTNAG 4 cut(s) 557, 903, 1135, 1163
HpySE526I ACGT 5 cut(s) 61, 165, 522, 714, 1104
Hsp92I GRCGYC 2 cut(s) 209, 478
Hsp92II CATG 5 cut(s) 225, 489, 636, 649, 1067
HspAI GCGC 1 cut(s) 599
KpnI GGTACC 1 cut(s) 579
KspI CCGCGG 1 cut(s) 876
Kzo9I GATC 2 cut(s) 560, 857
LmnI GCTCC 3 cut(s) 175, 617, 930
Lsp1109I GCAGC 4 cut(s) 358, 448, 1036, 1179
LweI GCATC 4 cut(s) 127, 586, 748, 906
MaeI CTAG 2 cut(s) 96, 942
MaeII ACGT 5 cut(s) 61, 165, 522, 714, 1104
MaeIII GTNAC 3 cut(s) 625, 657, 991
MalI GATC 2 cut(s) 562, 859
MboI GATC 2 cut(s) 560, 857
MboII GAAGA 1 cut(s) 844
MfeI CAATTG 1 cut(s) 268
MhlI GDGCHC 1 cut(s) 796
MluCI AATT 9 cut(s) 74, 201, 268, 511, 565, 581, 809, 823, 1012
MlyI GAGTC 1 cut(s) 1169
MmeI TCCRAC 2 cut(s) 32, 529
MseI TTAA 3 cut(s) 66, 422, 926
MslI CAYNNNNRTG 2 cut(s) 943, 1068
MspA1I CMGCKG 3 cut(s) 875, 964, 1021
MspI CCGG 2 cut(s) 106, 173
MspR9I CCNGG 7 cut(s) 107, 351, 358, 398, 416, 786, 1145
MunI CAATTG 1 cut(s) 268
MvaI CCWGG 6 cut(s) 351, 358, 398, 416, 786, 1145
MvnI CGCG 1 cut(s) 875
MwoI GCNNNNNNNGC 5 cut(s) 175, 486, 767, 1145, 1167
NciI CCSGG 1 cut(s) 107
NdeII GATC 2 cut(s) 560, 857
NlaIII CATG 5 cut(s) 225, 489, 636, 649, 1067
NlaIV GGNNCC 7 cut(s) 355, 577, 619, 724, 793, 871, 939
NmuCI GTSAC 2 cut(s) 625, 657
NsbI TGCGCA 1 cut(s) 600
NspI RCATGY 2 cut(s) 489, 636
OliI CACNNNNGTG 1 cut(s) 943
PaeR7I CTCGAG 1 cut(s) 1079
PasI CCCWGGG 1 cut(s) 357
PciI ACATGT 1 cut(s) 632
PkrI GCNGC 5 cut(s) 171, 348, 463, 1051, 1169
PleI GAGTC 1 cut(s) 1169
PpsI GAGTC 1 cut(s) 1169
PpuMI RGGWCCY 1 cut(s) 869
PscI ACATGT 1 cut(s) 632
Psp5II RGGWCCY 1 cut(s) 869
Psp6I CCWGG 6 cut(s) 349, 356, 396, 414, 784, 1143
PspGI CCWGG 6 cut(s) 349, 356, 396, 414, 784, 1143
PspN4I GGNNCC 7 cut(s) 355, 577, 619, 724, 793, 871, 939
PspPI GGNCC 3 cut(s) 353, 691, 869
PspPPI RGGWCCY 1 cut(s) 869
PvuII CAGCTG 1 cut(s) 1021
RsaI GTAC 2 cut(s) 577, 753
RsaNI GTAC 2 cut(s) 576, 752
RseI CAYNNNNRTG 2 cut(s) 943, 1068
SacII CCGCGG 1 cut(s) 876
SaqAI TTAA 3 cut(s) 66, 422, 926
SatI GCNGC 5 cut(s) 170, 347, 462, 1050, 1168
Sau3AI GATC 2 cut(s) 560, 857
Sau96I GGNCC 3 cut(s) 353, 691, 869
SchI GAGTC 1 cut(s) 1169
ScrFI CCNGG 7 cut(s) 107, 351, 358, 398, 416, 786, 1145
SduI GDGCHC 1 cut(s) 796
SfaNI GCATC 4 cut(s) 127, 586, 748, 906
Sfr274I CTCGAG 1 cut(s) 1079
Sfr303I CCGCGG 1 cut(s) 876
SgrBI CCGCGG 1 cut(s) 876
SinI GGWCC 2 cut(s) 353, 869
SlaI CTCGAG 1 cut(s) 1079
SmiMI CAYNNNNRTG 2 cut(s) 943, 1068
SmlI CTYRAG 1 cut(s) 1079
SmoI CTYRAG 1 cut(s) 1079
Sse9I AATT 9 cut(s) 74, 201, 268, 511, 565, 581, 809, 823, 1012
SsiI CCGC 5 cut(s) 170, 232, 873, 875, 964
SspMI CTAG 2 cut(s) 96, 942
StyD4I CCNGG 7 cut(s) 105, 349, 356, 396, 414, 784, 1143
StyI CCWWGG 1 cut(s) 941
TaaI ACNGT 1 cut(s) 539
TaiI ACGT 5 cut(s) 64, 168, 525, 717, 1107
TaqI TCGA 3 cut(s) 951, 985, 1080
TasI AATT 9 cut(s) 74, 201, 268, 511, 565, 581, 809, 823, 1012
TauI GCSGC 1 cut(s) 172
Tru1I TTAA 3 cut(s) 66, 422, 926
Tru9I TTAA 3 cut(s) 66, 422, 926
TscAI CASTG 1 cut(s) 544
TseFI GTSAC 2 cut(s) 625, 657
TseI GCWGC 4 cut(s) 346, 461, 1049, 1167
Tsp45I GTSAC 2 cut(s) 625, 657
TspDTI ATGAA 4 cut(s) 299, 662, 758, 924
TspGWI ACGGA 1 cut(s) 1056
TspRI CASTG 1 cut(s) 544
VpaK11BI GGWCC 2 cut(s) 353, 869
XagI CCTNNNNNAGG 1 cut(s) 127
XapI RAATTY 6 cut(s) 74, 511, 565, 581, 809, 1012
XceI RCATGY 2 cut(s) 489, 636
XhoI CTCGAG 1 cut(s) 1079
XmaJI CCTAGG 1 cut(s) 941
XmiI GTMKAC 2 cut(s) 16, 475
XspI CTAG 2 cut(s) 96, 942
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.