Belongs to the TUB family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 338 |
| AarI | CACCTGC | 1 cut(s) | 455 |
| Acc36I | ACCTGC | 1 cut(s) | 455 |
| Acc65I | GGTACC | 1 cut(s) | 1113 |
| AccB1I | GGYRCC | 2 cut(s) | 231, 1113 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 1031 |
| AccI | GTMKAC | 1 cut(s) | 16 |
| AccII | CGCG | 3 cut(s) | 187, 427, 441 |
| AciI | CCGC | 5 cut(s) | 49, 174, 182, 185, 187 |
| AcoI | YGGCCR | 3 cut(s) | 75, 179, 888 |
| AcsI | RAATTY | 2 cut(s) | 616, 821 |
| AcuI | CTGAAG | 4 cut(s) | 5, 119, 747, 978 |
| AfaI | GTAC | 3 cut(s) | 709, 739, 1115 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 64, 241, 793, 1031 |
| AgeI | ACCGGT | 1 cut(s) | 1110 |
| AgsI | TTSAA | 4 cut(s) | 622, 770, 967, 1143 |
| AjnI | CCWGG | 3 cut(s) | 304, 772, 1131 |
| AluBI | AGCT | 7 cut(s) | 108, 133, 225, 265, 277, 1040, 1158 |
| AluI | AGCT | 7 cut(s) | 108, 133, 225, 265, 277, 1040, 1158 |
| Alw21I | GWGCWC | 1 cut(s) | 267 |
| Alw26I | GTCTC | 2 cut(s) | 469, 933 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 133 |
| AoxI | GGCC | 8 cut(s) | 75, 162, 171, 179, 608, 881, 888, 1043 |
| ApeKI | GCWGC | 8 cut(s) | 46, 108, 133, 301, 422, 498, 562, 1155 |
| ApoI | RAATTY | 2 cut(s) | 616, 821 |
| AsiGI | ACCGGT | 1 cut(s) | 1110 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 825 |
| Asp718I | GGTACC | 1 cut(s) | 1113 |
| AspA2I | CCTAGG | 2 cut(s) | 635, 932 |
| AspLEI | GCGC | 4 cut(s) | 209, 427, 429, 443 |
| AspS9I | GGNCC | 7 cut(s) | 55, 308, 640, 719, 881, 1034, 1043 |
| AsuC2I | CCSGG | 1 cut(s) | 40 |
| AsuHPI | GGTGA | 4 cut(s) | 101, 163, 229, 624 |
| AvaII | GGWCC | 5 cut(s) | 55, 308, 640, 719, 1034 |
| AvrII | CCTAGG | 2 cut(s) | 635, 932 |
| AxyI | CCTNAGG | 1 cut(s) | 126 |
| BalI | TGGCCA | 1 cut(s) | 890 |
| BanI | GGYRCC | 2 cut(s) | 231, 1113 |
| BanII | GRGCYC | 2 cut(s) | 118, 267 |
| Bbv12I | GWGCWC | 1 cut(s) | 267 |
| BbvCI | CCTCAGC | 2 cut(s) | 137, 1151 |
| BbvI | GCAGC | 8 cut(s) | 58, 95, 120, 313, 409, 510, 574, 1167 |
| BccI | CCATC | 3 cut(s) | 296, 551, 838 |
| BciT130I | CCWGG | 3 cut(s) | 306, 774, 1133 |
| BcnI | CCSGG | 1 cut(s) | 40 |
| BcoDI | GTCTC | 2 cut(s) | 469, 933 |
| BfaI | CTAG | 3 cut(s) | 606, 636, 933 |
| BfmI | CTRYAG | 1 cut(s) | 326 |
| BfuAI | ACCTGC | 1 cut(s) | 455 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 170 |
| BlnI | CCTAGG | 2 cut(s) | 635, 932 |
| Bme1390I | CCNGG | 4 cut(s) | 40, 306, 774, 1133 |
| Bme18I | GGWCC | 5 cut(s) | 55, 308, 640, 719, 1034 |
| BmgT120I | GGNCC | 7 cut(s) | 55, 308, 640, 719, 881, 1034, 1043 |
| BmiI | GGNNCC | 9 cut(s) | 233, 258, 347, 580, 641, 642, 697, 720, 1115 |
| BmrFI | CCNGG | 4 cut(s) | 40, 306, 774, 1133 |
| BmrI | ACTGGG | 1 cut(s) | 279 |
| BmsI | GCATC | 2 cut(s) | 111, 736 |
| BmuI | ACTGGG | 1 cut(s) | 279 |
| BpmI | CTGGAG | 4 cut(s) | 247, 795, 935, 1008 |
| Bpu10I | CCTNAGC | 3 cut(s) | 137, 261, 1151 |
| BpuMI | CCSGG | 1 cut(s) | 40 |
| BsaBI | GATNNNNATC | 1 cut(s) | 853 |
| BsaWI | WCCGGW | 1 cut(s) | 1110 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 64, 241, 793, 1031 |
| Bse118I | RCCGGY | 2 cut(s) | 209, 1110 |
| Bse1I | ACTGG | 5 cut(s) | 274, 611, 659, 673, 952 |
| Bse21I | CCTNAGG | 1 cut(s) | 126 |
| Bse8I | GATNNNNATC | 1 cut(s) | 853 |
| BseBI | CCWGG | 3 cut(s) | 306, 774, 1133 |
| BseGI | GGATG | 5 cut(s) | 322, 669, 751, 830, 1062 |
| BseJI | GATNNNNATC | 1 cut(s) | 853 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 64, 241, 793, 1031 |
| BseMII | CTCAG | 5 cut(s) | 128, 131, 140, 252, 1165 |
| BseNI | ACTGG | 5 cut(s) | 274, 611, 659, 673, 952 |
| BsePI | GCGCGC | 1 cut(s) | 425 |
| BseRI | GAGGAG | 1 cut(s) | 792 |
| BseX3I | CGGCCG | 1 cut(s) | 75 |
| BseXI | GCAGC | 8 cut(s) | 58, 95, 120, 313, 409, 510, 574, 1167 |
| BseYI | CCCAGC | 2 cut(s) | 111, 1040 |
| BsgI | GTGCAG | 1 cut(s) | 433 |
| Bsh1236I | CGCG | 3 cut(s) | 187, 427, 441 |
| Bsh1285I | CGRYCG | 2 cut(s) | 44, 78 |
| BshFI | GGCC | 8 cut(s) | 77, 164, 173, 181, 610, 883, 890, 1045 |
| BshNI | GGYRCC | 2 cut(s) | 231, 1113 |
| BshTI | ACCGGT | 1 cut(s) | 1110 |
| BsiEI | CGRYCG | 2 cut(s) | 44, 78 |
| BsiHKAI | GWGCWC | 1 cut(s) | 267 |
| BsiSI | CCGG | 5 cut(s) | 40, 210, 220, 576, 1111 |
| BslFI | GGGAC | 1 cut(s) | 626 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 64, 241, 793, 1031 |
| BsmAI | GTCTC | 2 cut(s) | 469, 933 |
| BsmFI | GGGAC | 1 cut(s) | 626 |
| BsmI | GAATGC | 2 cut(s) | 735, 1127 |
| BsnI | GGCC | 8 cut(s) | 77, 164, 173, 181, 610, 883, 890, 1045 |
| Bsp1286I | GDGCHC | 2 cut(s) | 118, 267 |
| Bsp1407I | TGTACA | 1 cut(s) | 707 |
| Bsp143I | GATC | 6 cut(s) | 246, 319, 521, 848, 1059, 1170 |
| Bsp19I | CCATGG | 3 cut(s) | 741, 973, 1101 |
| BspACI | CCGC | 5 cut(s) | 49, 174, 182, 185, 187 |
| BspANI | GGCC | 8 cut(s) | 77, 164, 173, 181, 610, 883, 890, 1045 |
| BspCNI | CTCAG | 5 cut(s) | 129, 130, 139, 253, 1164 |
| BspFNI | CGCG | 3 cut(s) | 187, 427, 441 |
| BspLI | GGNNCC | 9 cut(s) | 233, 258, 347, 580, 641, 642, 697, 720, 1115 |
| BspMAI | CTGCAG | 1 cut(s) | 330 |
| BspMI | ACCTGC | 1 cut(s) | 455 |
| BspQI | GCTCTTC | 1 cut(s) | 185 |
| BspT107I | GGYRCC | 2 cut(s) | 231, 1113 |
| BsrFI | RCCGGY | 2 cut(s) | 209, 1110 |
| BsrGI | TGTACA | 1 cut(s) | 707 |
| BsrI | ACTGG | 5 cut(s) | 274, 611, 659, 673, 952 |
| BssAI | RCCGGY | 2 cut(s) | 209, 1110 |
| BssHII | GCGCGC | 1 cut(s) | 425 |
| BssMI | GATC | 6 cut(s) | 246, 319, 521, 848, 1059, 1170 |
| BssNAI | GTATAC | 1 cut(s) | 17 |
| BssT1I | CCWWGG | 9 cut(s) | 240, 311, 376, 635, 722, 741, 932, 973, 1101 |
| Bst1107I | GTATAC | 1 cut(s) | 17 |
| Bst2UI | CCWGG | 3 cut(s) | 306, 774, 1133 |
| Bst4CI | ACNGT | 2 cut(s) | 350, 988 |
| Bst6I | CTCTTC | 1 cut(s) | 185 |
| BstAUI | TGTACA | 1 cut(s) | 707 |
| BstC8I | GCNNGC | 4 cut(s) | 70, 205, 427, 892 |
| BstDEI | CTNAG | 5 cut(s) | 117, 126, 137, 261, 1151 |
| BstDSI | CCRYGG | 4 cut(s) | 185, 741, 973, 1101 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 239 |
| BstF5I | GGATG | 5 cut(s) | 322, 669, 751, 830, 1062 |
| BstFNI | CGCG | 3 cut(s) | 187, 427, 441 |
| BstHHI | GCGC | 4 cut(s) | 209, 427, 429, 443 |
| BstKTI | GATC | 6 cut(s) | 249, 322, 524, 851, 1062, 1173 |
| BstMAI | GTCTC | 2 cut(s) | 469, 933 |
| BstMBI | GATC | 6 cut(s) | 246, 319, 521, 848, 1059, 1170 |
| BstMCI | CGRYCG | 2 cut(s) | 44, 78 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 74, 130, 170, 447, 755, 1133, 1155 |
| BstNI | CCWGG | 3 cut(s) | 306, 774, 1133 |
| BstNSI | RCATGY | 1 cut(s) | 476 |
| BstSCI | CCNGG | 4 cut(s) | 38, 304, 772, 1131 |
| BstSFI | CTRYAG | 1 cut(s) | 326 |
| BstUI | CGCG | 3 cut(s) | 187, 427, 441 |
| BstV1I | GCAGC | 8 cut(s) | 58, 95, 120, 313, 409, 510, 574, 1167 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 902 |
| BstZ17I | GTATAC | 1 cut(s) | 17 |
| BstZI | CGGCCG | 1 cut(s) | 75 |
| Bsu36I | CCTNAGG | 1 cut(s) | 126 |
| BsuRI | GGCC | 8 cut(s) | 77, 164, 173, 181, 610, 883, 890, 1045 |
| BtgI | CCRYGG | 4 cut(s) | 185, 741, 973, 1101 |
| BtsCI | GGATG | 5 cut(s) | 322, 669, 751, 830, 1062 |
| BtsI | GCAGTG | 2 cut(s) | 335, 740 |
| BtsIMutI | CAGTG | 7 cut(s) | 95, 267, 335, 450, 740, 959, 1002 |
| BveI | ACCTGC | 1 cut(s) | 455 |
| Cac8I | GCNNGC | 4 cut(s) | 70, 205, 427, 892 |
| CaiI | CAGNNNCTG | 1 cut(s) | 133 |
| CfoI | GCGC | 4 cut(s) | 209, 427, 429, 443 |
| Cfr10I | RCCGGY | 2 cut(s) | 209, 1110 |
| Cfr13I | GGNCC | 7 cut(s) | 55, 308, 640, 719, 881, 1034, 1043 |
| Cfr42I | CCGCGG | 1 cut(s) | 188 |
| CseI | GACGC | 1 cut(s) | 184 |
| Csp6I | GTAC | 3 cut(s) | 708, 738, 1114 |
| CspAI | ACCGGT | 1 cut(s) | 1110 |
| CspCI | CAANNNNNGTGG | 4 cut(s) | 748, 783, 846, 881 |
| CviQI | GTAC | 3 cut(s) | 708, 738, 1114 |
| DdeI | CTNAG | 5 cut(s) | 117, 126, 137, 261, 1151 |
| DpnI | GATC | 6 cut(s) | 248, 321, 523, 850, 1061, 1172 |
| DpnII | GATC | 6 cut(s) | 246, 319, 521, 848, 1059, 1170 |
| EaeI | YGGCCR | 3 cut(s) | 75, 179, 888 |
| EagI | CGGCCG | 1 cut(s) | 75 |
| Eam1104I | CTCTTC | 1 cut(s) | 185 |
| EarI | CTCTTC | 1 cut(s) | 185 |
| Ecl136II | GAGCTC | 1 cut(s) | 265 |
| EclXI | CGGCCG | 1 cut(s) | 75 |
| Eco130I | CCWWGG | 9 cut(s) | 240, 311, 376, 635, 722, 741, 932, 973, 1101 |
| Eco147I | AGGCCT | 1 cut(s) | 164 |
| Eco24I | GRGCYC | 2 cut(s) | 118, 267 |
| Eco47I | GGWCC | 5 cut(s) | 55, 308, 640, 719, 1034 |
| Eco52I | CGGCCG | 1 cut(s) | 75 |
| Eco53kI | GAGCTC | 1 cut(s) | 265 |
| Eco57I | CTGAAG | 4 cut(s) | 5, 119, 747, 978 |
| Eco81I | CCTNAGG | 1 cut(s) | 126 |
| EcoICRI | GAGCTC | 1 cut(s) | 265 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 239 |
| EcoO109I | RGGNCCY | 2 cut(s) | 640, 881 |
| EcoRI | GAATTC | 1 cut(s) | 821 |
| EcoRII | CCWGG | 3 cut(s) | 304, 772, 1131 |
| EcoT14I | CCWWGG | 9 cut(s) | 240, 311, 376, 635, 722, 741, 932, 973, 1101 |
| EcoT38I | GRGCYC | 2 cut(s) | 118, 267 |
| ErhI | CCWWGG | 9 cut(s) | 240, 311, 376, 635, 722, 741, 932, 973, 1101 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 266, 298 |
| FaqI | GGGAC | 1 cut(s) | 626 |
| FauI | CCCGC | 1 cut(s) | 42 |
| FauNDI | CATATG | 1 cut(s) | 808 |
| FblI | GTMKAC | 1 cut(s) | 16 |
| FokI | GGATG | 5 cut(s) | 329, 656, 758, 817, 1049 |
| FriOI | GRGCYC | 2 cut(s) | 118, 267 |
| FspBI | CTAG | 3 cut(s) | 606, 636, 933 |
| GlaI | GCGC | 4 cut(s) | 208, 426, 428, 442 |
| GsaI | CCCAGC | 2 cut(s) | 115, 1044 |
| GsuI | CTGGAG | 4 cut(s) | 247, 795, 935, 1008 |
| HaeIII | GGCC | 8 cut(s) | 77, 164, 173, 181, 610, 883, 890, 1045 |
| HapII | CCGG | 5 cut(s) | 40, 210, 220, 576, 1111 |
| HgaI | GACGC | 1 cut(s) | 184 |
| HhaI | GCGC | 4 cut(s) | 209, 427, 429, 443 |
| Hin6I | GCGC | 4 cut(s) | 207, 425, 427, 441 |
| HinP1I | GCGC | 4 cut(s) | 207, 425, 427, 441 |
| HinfI | GANTC | 5 cut(s) | 62, 80, 92, 156, 568 |
| HpaII | CCGG | 5 cut(s) | 40, 210, 220, 576, 1111 |
| HphI | GGTGA | 4 cut(s) | 101, 163, 229, 624 |
| Hpy166II | GTNNAC | 3 cut(s) | 17, 546, 738 |
| Hpy188I | TCNGA | 3 cut(s) | 120, 169, 848 |
| Hpy188III | TCNNGA | 5 cut(s) | 23, 128, 260, 699, 1025 |
| Hpy8I | GTNNAC | 3 cut(s) | 17, 546, 738 |
| Hpy99I | CGWCG | 2 cut(s) | 200, 203 |
| HpyAV | CCTTC | 2 cut(s) | 292, 836 |
| HpyCH4III | ACNGT | 2 cut(s) | 350, 988 |
| HpyCH4V | TGCA | 5 cut(s) | 328, 392, 450, 733, 818 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 74, 130, 170, 447, 755, 1133, 1155 |
| HpyF3I | CTNAG | 5 cut(s) | 117, 126, 137, 261, 1151 |
| HspAI | GCGC | 4 cut(s) | 207, 425, 427, 441 |
| KflI | GGGWCCC | 1 cut(s) | 640 |
| KpnI | GGTACC | 1 cut(s) | 1117 |
| KspI | CCGCGG | 1 cut(s) | 188 |
| Kzo9I | GATC | 6 cut(s) | 246, 319, 521, 848, 1059, 1170 |
| LguI | GCTCTTC | 1 cut(s) | 185 |
| LmnI | GCTCC | 5 cut(s) | 130, 222, 262, 495, 578 |
| Lsp1109I | GCAGC | 8 cut(s) | 58, 95, 120, 313, 409, 510, 574, 1167 |
| LweI | GCATC | 2 cut(s) | 111, 736 |
| MaeI | CTAG | 3 cut(s) | 606, 636, 933 |
| MaeIII | GTNAC | 2 cut(s) | 93, 982 |
| MalI | GATC | 6 cut(s) | 248, 321, 523, 850, 1061, 1172 |
| MboI | GATC | 6 cut(s) | 246, 319, 521, 848, 1059, 1170 |
| MboII | GAAGA | 2 cut(s) | 202, 1069 |
| MhlI | GDGCHC | 2 cut(s) | 118, 267 |
| MlsI | TGGCCA | 1 cut(s) | 890 |
| MluCI | AATT | 4 cut(s) | 515, 616, 821, 1070 |
| MluNI | TGGCCA | 1 cut(s) | 890 |
| MlyI | GAGTC | 4 cut(s) | 56, 89, 101, 562 |
| Mox20I | TGGCCA | 1 cut(s) | 890 |
| MroXI | GAANNNNTTC | 1 cut(s) | 825 |
| MscI | TGGCCA | 1 cut(s) | 890 |
| MseI | TTAA | 1 cut(s) | 917 |
| MslI | CAYNNNNRTG | 1 cut(s) | 477 |
| Msp20I | TGGCCA | 1 cut(s) | 890 |
| MspA1I | CMGCKG | 3 cut(s) | 49, 187, 1040 |
| MspI | CCGG | 5 cut(s) | 40, 210, 220, 576, 1111 |
| MspR9I | CCNGG | 4 cut(s) | 40, 306, 774, 1133 |
| Mva1269I | GAATGC | 2 cut(s) | 735, 1127 |
| MvaI | CCWGG | 3 cut(s) | 306, 774, 1133 |
| MvnI | CGCG | 3 cut(s) | 187, 427, 441 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 74, 130, 170, 447, 755, 1133, 1155 |
| NciI | CCSGG | 1 cut(s) | 40 |
| NcoI | CCATGG | 3 cut(s) | 741, 973, 1101 |
| NdeI | CATATG | 1 cut(s) | 808 |
| NdeII | GATC | 6 cut(s) | 246, 319, 521, 848, 1059, 1170 |
| NlaIV | GGNNCC | 9 cut(s) | 233, 258, 347, 580, 641, 642, 697, 720, 1115 |
| NmeAIII | GCCGAG | 3 cut(s) | 44, 53, 103 |
| NmuCI | GTSAC | 1 cut(s) | 93 |
| NspI | RCATGY | 1 cut(s) | 476 |
| PaqCI | CACCTGC | 1 cut(s) | 455 |
| PauI | GCGCGC | 1 cut(s) | 425 |
| PceI | AGGCCT | 1 cut(s) | 164 |
| PciSI | GCTCTTC | 1 cut(s) | 185 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 165 |
| PctI | GAATGC | 2 cut(s) | 735, 1127 |
| PdmI | GAANNNNTTC | 1 cut(s) | 825 |
| PfeI | GAWTC | 1 cut(s) | 156 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 1031 |
| PinAI | ACCGGT | 1 cut(s) | 1110 |
| PleI | GAGTC | 4 cut(s) | 56, 88, 100, 562 |
| PpsI | GAGTC | 4 cut(s) | 56, 88, 100, 562 |
| PpuMI | RGGWCCY | 1 cut(s) | 640 |
| PsiI | TTATAA | 1 cut(s) | 338 |
| Psp124BI | GAGCTC | 1 cut(s) | 267 |
| Psp5II | RGGWCCY | 1 cut(s) | 640 |
| Psp6I | CCWGG | 3 cut(s) | 304, 772, 1131 |
| PspFI | CCCAGC | 2 cut(s) | 111, 1040 |
| PspGI | CCWGG | 3 cut(s) | 304, 772, 1131 |
| PspN4I | GGNNCC | 9 cut(s) | 233, 258, 347, 580, 641, 642, 697, 720, 1115 |
| PspPI | GGNCC | 7 cut(s) | 55, 308, 640, 719, 881, 1034, 1043 |
| PspPPI | RGGWCCY | 1 cut(s) | 640 |
| PstI | CTGCAG | 1 cut(s) | 330 |
| PstNI | CAGNNNCTG | 1 cut(s) | 133 |
| PteI | GCGCGC | 1 cut(s) | 425 |
| PvuII | CAGCTG | 1 cut(s) | 1040 |
| RsaI | GTAC | 3 cut(s) | 709, 739, 1115 |
| RsaNI | GTAC | 3 cut(s) | 708, 738, 1114 |
| RseI | CAYNNNNRTG | 1 cut(s) | 477 |
| SacI | GAGCTC | 1 cut(s) | 267 |
| SacII | CCGCGG | 1 cut(s) | 188 |
| SapI | GCTCTTC | 1 cut(s) | 185 |
| SaqAI | TTAA | 1 cut(s) | 917 |
| Sau3AI | GATC | 6 cut(s) | 246, 319, 521, 848, 1059, 1170 |
| Sau96I | GGNCC | 7 cut(s) | 55, 308, 640, 719, 881, 1034, 1043 |
| SchI | GAGTC | 4 cut(s) | 56, 89, 101, 562 |
| ScrFI | CCNGG | 4 cut(s) | 40, 306, 774, 1133 |
| SduI | GDGCHC | 2 cut(s) | 118, 267 |
| SfaNI | GCATC | 2 cut(s) | 111, 736 |
| SfcI | CTRYAG | 1 cut(s) | 326 |
| SfiI | GGCCNNNNNGGCC | 1 cut(s) | 170 |
| Sfr303I | CCGCGG | 1 cut(s) | 188 |
| SgrAI | CRCCGGYG | 1 cut(s) | 209 |
| SgrBI | CCGCGG | 1 cut(s) | 188 |
| SinI | GGWCC | 5 cut(s) | 55, 308, 640, 719, 1034 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 477 |
| Sse9I | AATT | 4 cut(s) | 515, 616, 821, 1070 |
| SseBI | AGGCCT | 1 cut(s) | 164 |
| SsiI | CCGC | 5 cut(s) | 49, 174, 182, 185, 187 |
| SspMI | CTAG | 3 cut(s) | 606, 636, 933 |
| SstI | GAGCTC | 1 cut(s) | 267 |
| StuI | AGGCCT | 1 cut(s) | 164 |
| StyD4I | CCNGG | 4 cut(s) | 38, 304, 772, 1131 |
| StyI | CCWWGG | 9 cut(s) | 240, 311, 376, 635, 722, 741, 932, 973, 1101 |
| TaaI | ACNGT | 2 cut(s) | 350, 988 |
| TaqI | TCGA | 2 cut(s) | 5, 159 |
| TasI | AATT | 4 cut(s) | 515, 616, 821, 1070 |
| TatI | WGTACW | 1 cut(s) | 707 |
| TauI | GCSGC | 3 cut(s) | 176, 184, 187 |
| TfiI | GAWTC | 1 cut(s) | 156 |
| Tru1I | TTAA | 1 cut(s) | 917 |
| Tru9I | TTAA | 1 cut(s) | 917 |
| TscAI | CASTG | 7 cut(s) | 102, 274, 335, 457, 740, 959, 1002 |
| TseFI | GTSAC | 1 cut(s) | 93 |
| TseI | GCWGC | 8 cut(s) | 46, 108, 133, 301, 422, 498, 562, 1155 |
| Tsp45I | GTSAC | 1 cut(s) | 93 |
| TspDTI | ATGAA | 3 cut(s) | 44, 693, 795 |
| TspGWI | ACGGA | 2 cut(s) | 283, 358 |
| TspRI | CASTG | 7 cut(s) | 102, 274, 335, 457, 740, 959, 1002 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 1031 |
| VpaK11BI | GGWCC | 5 cut(s) | 55, 308, 640, 719, 1034 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 239 |
| XapI | RAATTY | 2 cut(s) | 616, 821 |
| XceI | RCATGY | 1 cut(s) | 476 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 65 |
| XmaJI | CCTAGG | 2 cut(s) | 635, 932 |
| XmiI | GTMKAC | 1 cut(s) | 16 |
| XmnI | GAANNNNTTC | 1 cut(s) | 825 |
| XspI | CTAG | 3 cut(s) | 606, 636, 933 |