pycom11g26220

Tubby-like F-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0001645
Physical Location & Seq
Reverse (-)
28835977 .. 28838108
2132 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom11g26220.2

Sequence Viewer

Length: 1209 bp
ATGTCTTTTAAGAGTATGATTCAAGATATGAAAGGAGAGTTGGGGAGCATTTCGAGGAAAAGCTTTGATGTTAAATTTGGAATGAGATCGAGGTCTCAGCGGGTGGTTCAGGATACCTCCTTCAGGATTAATGCTTTGAAGCAGAGTTGTTGGGCAAACATGCCGCCGGAGCTTTTGAGGGACGTGTTGATGAGAATAGAGGCTTCTGATGATACATGGCCTCCGAGGAAAAATGTGGTCTGCTGTGCTGGTGTTTGCAGGAATTGGAGAGAAATCATGAAAGAGATTGTGAAATCCCCAGAAGTTTCGGGCAAGCTGACATTCCCAATCTCTTTGAAGCAGCCTGGTCCAAGGAACTCCCTTCTGCAGTGTTATATAAAGCGATGTCGTAGCAACCAGACATATTATCTTTTTCTGGGTTTAAGTCAAGCCTCAACTGATGATGGGAAGTTTCTTCTTGCGGCAAAAAAATGTAGACGCCCAACCTGCACAGACTATACCATCTCTTTGAAGTCTGAAGATGTGTCAAAAGGGAACAGTGCCTTTGTTGGAAAGCTCAGATCAAATTTTCTGGGCACTAAATTCACAATCTATGATGCGCAACCTACTAACAGTGGAGCCAAAGTCACCAAATGTCGTTCAACCAGGCTTGTGAATAAAGTCTCCCCCAGAGTTCCTGCTGGCAACTATCCTGTGGCCCATATTGCTTATGAGTTGAACGTCTTGGGTTCCAGAGGTCCGAGGAGAATGCAGTGTGTAATGGATTCCATCCCTGCCCGTGCTGTTGAGCCAGGAGGTGTGGCACCCACACAGACTGAATTTCTGCATGGCAGCCCGGATAGTTTTCCATCCATCCCATTTTCCAGATCAACATCAACCCGCACAAAGAATTTCTCATCTCCACTAGAGTCTGGTGAGAATGAAGGCATGCTAGTATTGAGAAACAAGGCCCCTAGGTGGCACGAACAACTCCAGTGCTGGTGTCTCAACTTCAATGGACGAGTAACAGTTGCCTCAGTAAAAAATTTTCAGCTGGTTGCTTCTCCGGAAAATGGAGTTGCTGCGGGCCAGGAGCATGAAAATGTCATCCTTCAGTTTGGAAAAGTGGGTAAGGATGTATTCACCATGGATTATCGGTATCCAATCTCAGCCTTCCAGGCATTTGCCATTTGCCTTAGCAGCTTCGACACCAAGATTGCTTGCGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005543 GO:0008289 GO:0035091 GO:0043167 GO:0043168
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box Tub

Protein Analysis

403

Amino Acids

44.87

Weight (kDa)

9.58

Isoelectric Point (pI)

50.68

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 600
Acc36I ACCTGC 1 cut(s) 494
AccB1I GGYRCC 1 cut(s) 802
AccI GTMKAC 1 cut(s) 475
AccIII TCCGGA 1 cut(s) 1045
AciI CCGC 5 cut(s) 100, 164, 461, 880, 1064
AcsI RAATTY 6 cut(s) 74, 565, 581, 818, 889, 1024
AcuI CTGAAG 3 cut(s) 106, 537, 1076
AcyI GRCGYC 1 cut(s) 478
AfiI CCNNNNNNNGG 3 cut(s) 123, 957, 1052
AflIII ACRYGT 1 cut(s) 183
AgsI TTSAA 7 cut(s) 23, 139, 337, 511, 642, 718, 994
AjiI CACGTC 1 cut(s) 184
AjnI CCWGG 5 cut(s) 343, 644, 790, 1068, 1155
AjuI GAANNNNNNNTTGG 2 cut(s) 23, 55
AluBI AGCT 6 cut(s) 63, 172, 316, 556, 1033, 1182
AluI AGCT 6 cut(s) 63, 172, 316, 556, 1033, 1182
Alw26I GTCTC 3 cut(s) 99, 667, 989
Aor13HI TCCGGA 1 cut(s) 1045
AoxI GGCC 4 cut(s) 218, 696, 948, 1066
ApeKI GCWGC 4 cut(s) 340, 831, 1061, 1179
ApoI RAATTY 6 cut(s) 74, 565, 581, 818, 889, 1024
AseI ATTAAT 1 cut(s) 129
AspA2I CCTAGG 1 cut(s) 953
AspLEI GCGC 1 cut(s) 601
AspS9I GGNCC 5 cut(s) 347, 697, 737, 949, 1066
AsuC2I CCSGG 1 cut(s) 836
AsuHPI GGTGA 3 cut(s) 619, 926, 1114
AvaII GGWCC 2 cut(s) 347, 737
AvrII CCTAGG 1 cut(s) 953
BaeGI GKGCMC 1 cut(s) 578
BanI GGYRCC 1 cut(s) 802
BbvI GCAGC 4 cut(s) 352, 843, 1048, 1191
BccI CCATC 5 cut(s) 437, 509, 776, 856, 860
BcgI CGANNNNNNTGC 2 cut(s) 730, 764
BciT130I CCWGG 5 cut(s) 345, 646, 792, 1070, 1157
BciVI GTATCC 2 cut(s) 106, 1149
BcnI CCSGG 1 cut(s) 836
BcoDI GTCTC 3 cut(s) 99, 667, 989
BfaI CTAG 3 cut(s) 905, 932, 954
BfmI CTRYAG 1 cut(s) 365
BfuAI ACCTGC 1 cut(s) 494
BfuI GTATCC 2 cut(s) 106, 1149
BglI GCCNNNNNGGC 1 cut(s) 1157
BisI GCNGC 6 cut(s) 164, 341, 462, 832, 1062, 1180
BlnI CCTAGG 1 cut(s) 953
BlsI GCNGC 6 cut(s) 165, 342, 463, 833, 1063, 1181
Bme1390I CCNGG 6 cut(s) 345, 646, 792, 836, 1070, 1157
Bme18I GGWCC 2 cut(s) 347, 737
BmgBI CACGTC 1 cut(s) 184
BmgT120I GGNCC 5 cut(s) 347, 697, 737, 949, 1066
BmiI GGNNCC 4 cut(s) 619, 730, 804, 951
BmrFI CCNGG 6 cut(s) 345, 646, 792, 836, 1070, 1157
BmsI GCATC 1 cut(s) 586
BpmI CTGGAG 1 cut(s) 956
Bpu10I CCTNAGC 1 cut(s) 1175
BpuMI CCSGG 1 cut(s) 836
BsaBI GATNNNNATC 1 cut(s) 871
BsaHI GRCGYC 1 cut(s) 478
BsaI GGTCTC 1 cut(s) 99
BsaJI CCNNGG 5 cut(s) 224, 350, 740, 953, 1125
BsaWI WCCGGW 1 cut(s) 1045
BsaXI ACNNNNNCTCC 4 cut(s) 205, 235, 609, 639
Bsc4I CCNNNNNNNGG 3 cut(s) 123, 957, 1052
Bse1I ACTGG 1 cut(s) 973
Bse8I GATNNNNATC 1 cut(s) 871
BseAI TCCGGA 1 cut(s) 1045
BseBI CCWGG 5 cut(s) 345, 646, 792, 1070, 1157
BseDI CCNNGG 5 cut(s) 224, 350, 740, 953, 1125
BseGI GGATG 5 cut(s) 768, 848, 852, 1086, 1120
BseJI GATNNNNATC 1 cut(s) 871
BseLI CCNNNNNNNGG 3 cut(s) 123, 957, 1052
BseMII CTCAG 4 cut(s) 110, 571, 1029, 1161
BseNI ACTGG 1 cut(s) 973
BseRI GAGGAG 1 cut(s) 757
BseSI GKGCMC 1 cut(s) 578
BseXI GCAGC 4 cut(s) 352, 843, 1048, 1191
BsgI GTGCAG 1 cut(s) 472
BshFI GGCC 4 cut(s) 220, 698, 950, 1068
BshNI GGYRCC 1 cut(s) 802
BsiSI CCGG 3 cut(s) 167, 836, 1046
BslFI GGGAC 1 cut(s) 194
BslI CCNNNNNNNGG 3 cut(s) 123, 957, 1052
BsmAI GTCTC 3 cut(s) 99, 667, 989
BsmFI GGGAC 1 cut(s) 194
BsmI GAATGC 1 cut(s) 753
BsnI GGCC 4 cut(s) 220, 698, 950, 1068
Bso31I GGTCTC 1 cut(s) 99
Bsp1286I GDGCHC 1 cut(s) 578
Bsp13I TCCGGA 1 cut(s) 1045
Bsp143I GATC 3 cut(s) 86, 560, 866
Bsp19I CCATGG 1 cut(s) 1125
BspACI CCGC 5 cut(s) 100, 164, 461, 880, 1064
BspANI GGCC 4 cut(s) 220, 698, 950, 1068
BspCNI CTCAG 4 cut(s) 109, 570, 1028, 1160
BspEI TCCGGA 1 cut(s) 1045
BspHI TCATGA 1 cut(s) 276
BspLI GGNNCC 4 cut(s) 619, 730, 804, 951
BspMAI CTGCAG 1 cut(s) 369
BspMI ACCTGC 1 cut(s) 494
BspT107I GGYRCC 1 cut(s) 802
BspTNI GGTCTC 1 cut(s) 99
BsrI ACTGG 1 cut(s) 973
BssECI CCNNGG 5 cut(s) 224, 350, 740, 953, 1125
BssMI GATC 3 cut(s) 86, 560, 866
BssNI GRCGYC 1 cut(s) 478
BssT1I CCWWGG 3 cut(s) 350, 953, 1125
Bst2UI CCWGG 5 cut(s) 345, 646, 792, 1070, 1157
Bst4CI ACNGT 3 cut(s) 539, 614, 1009
BstACI GRCGYC 1 cut(s) 478
BstC8I GCNNGC 5 cut(s) 314, 682, 929, 1066, 1201
BstDEI CTNAG 5 cut(s) 96, 557, 1015, 1147, 1175
BstDSI CCRYGG 1 cut(s) 1125
BstENI CCTNNNNNAGG 1 cut(s) 121
BstF5I GGATG 5 cut(s) 768, 848, 852, 1086, 1120
BstHHI GCGC 1 cut(s) 601
BstKTI GATC 3 cut(s) 89, 563, 869
BstMAI GTCTC 3 cut(s) 99, 667, 989
BstMBI GATC 3 cut(s) 86, 560, 866
BstMWI GCNNNNNNNGC 5 cut(s) 169, 486, 704, 1157, 1179
BstNI CCWGG 5 cut(s) 345, 646, 792, 1070, 1157
BstNSI RCATGY 2 cut(s) 163, 931
BstSCI CCNGG 6 cut(s) 343, 644, 790, 834, 1068, 1155
BstSFI CTRYAG 1 cut(s) 365
BstSLI GKGCMC 1 cut(s) 578
BstV1I GCAGC 4 cut(s) 352, 843, 1048, 1191
BsuI GTATCC 2 cut(s) 106, 1149
BsuRI GGCC 4 cut(s) 220, 698, 950, 1068
BtgI CCRYGG 1 cut(s) 1125
BtgZI GCGATG 1 cut(s) 397
BtrI CACGTC 1 cut(s) 184
BtsCI GGATG 5 cut(s) 768, 848, 852, 1086, 1120
BtsI GCAGTG 2 cut(s) 374, 758
BtsIMutI CAGTG 5 cut(s) 374, 544, 619, 758, 980
BveI ACCTGC 1 cut(s) 494
Cac8I GCNNGC 5 cut(s) 314, 682, 929, 1066, 1201
CciI TCATGA 1 cut(s) 276
CfoI GCGC 1 cut(s) 601
Cfr13I GGNCC 5 cut(s) 347, 697, 737, 949, 1066
CseI GACGC 1 cut(s) 486
CviAII CATG 7 cut(s) 160, 216, 277, 827, 928, 1076, 1126
DdeI CTNAG 5 cut(s) 96, 557, 1015, 1147, 1175
DpnI GATC 3 cut(s) 88, 562, 868
DpnII GATC 3 cut(s) 86, 560, 866
Eco130I CCWWGG 3 cut(s) 350, 953, 1125
Eco31I GGTCTC 1 cut(s) 99
Eco47I GGWCC 2 cut(s) 347, 737
Eco57I CTGAAG 3 cut(s) 106, 537, 1076
EcoNI CCTNNNNNAGG 1 cut(s) 121
EcoO109I RGGNCCY 1 cut(s) 949
EcoRII CCWGG 5 cut(s) 343, 644, 790, 1068, 1155
EcoT14I CCWWGG 3 cut(s) 350, 953, 1125
ErhI CCWWGG 3 cut(s) 350, 953, 1125
FaeI CATG 7 cut(s) 163, 219, 280, 830, 931, 1079, 1129
FaqI GGGAC 1 cut(s) 194
FatI CATG 7 cut(s) 159, 215, 276, 826, 927, 1075, 1125
FauI CCCGC 3 cut(s) 93, 887, 1057
FblI GTMKAC 1 cut(s) 475
Fnu4HI GCNGC 6 cut(s) 164, 341, 462, 832, 1062, 1180
FokI GGATG 5 cut(s) 755, 835, 839, 1073, 1127
Fsp4HI GCNGC 6 cut(s) 164, 341, 462, 832, 1062, 1180
FspBI CTAG 3 cut(s) 905, 932, 954
FspI TGCGCA 1 cut(s) 600
GlaI GCGC 1 cut(s) 600
GluI GCNGC 6 cut(s) 164, 341, 462, 832, 1062, 1180
GsuI CTGGAG 1 cut(s) 956
HaeIII GGCC 4 cut(s) 220, 698, 950, 1068
HapII CCGG 3 cut(s) 167, 836, 1046
HgaI GACGC 1 cut(s) 486
HhaI GCGC 1 cut(s) 601
Hin1I GRCGYC 1 cut(s) 478
Hin1II CATG 7 cut(s) 163, 219, 280, 830, 931, 1079, 1129
Hin6I GCGC 1 cut(s) 599
HinP1I GCGC 1 cut(s) 599
HindIII AAGCTT 1 cut(s) 61
HinfI GANTC 3 cut(s) 19, 764, 908
HpaII CCGG 3 cut(s) 167, 836, 1046
HphI GGTGA 3 cut(s) 619, 926, 1114
Hpy166II GTNNAC 1 cut(s) 476
Hpy188I TCNGA 5 cut(s) 208, 225, 517, 560, 741
Hpy188III TCNNGA 7 cut(s) 23, 110, 124, 277, 732, 864, 1046
Hpy8I GTNNAC 1 cut(s) 476
HpyAV CCTTC 5 cut(s) 130, 371, 917, 1100, 1162
HpyCH4III ACNGT 3 cut(s) 539, 614, 1009
HpyCH4IV ACGT 2 cut(s) 183, 720
HpyCH4V TGCA 5 cut(s) 258, 367, 489, 751, 826
HpyF10VI GCNNNNNNNGC 5 cut(s) 169, 486, 704, 1157, 1179
HpyF3I CTNAG 5 cut(s) 96, 557, 1015, 1147, 1175
HpySE526I ACGT 2 cut(s) 183, 720
Hsp92I GRCGYC 1 cut(s) 478
Hsp92II CATG 7 cut(s) 163, 219, 280, 830, 931, 1079, 1129
HspAI GCGC 1 cut(s) 599
Kpn2I TCCGGA 1 cut(s) 1045
Kzo9I GATC 3 cut(s) 86, 560, 866
LmnI GCTCC 4 cut(s) 45, 169, 617, 1072
Lsp1109I GCAGC 4 cut(s) 352, 843, 1048, 1191
LweI GCATC 1 cut(s) 586
MaeI CTAG 3 cut(s) 905, 932, 954
MaeII ACGT 2 cut(s) 183, 720
MaeIII GTNAC 2 cut(s) 625, 1003
MalI GATC 3 cut(s) 88, 562, 868
MboI GATC 3 cut(s) 86, 560, 866
MboII GAAGA 2 cut(s) 446, 530
MhlI GDGCHC 1 cut(s) 578
MluCI AATT 7 cut(s) 74, 262, 565, 581, 818, 889, 1024
MlyI GAGTC 1 cut(s) 917
MmeI TCCRAC 1 cut(s) 529
MroI TCCGGA 1 cut(s) 1045
MseI TTAA 4 cut(s) 9, 72, 129, 422
MslI CAYNNNNRTG 1 cut(s) 1080
MspA1I CMGCKG 2 cut(s) 100, 1033
MspI CCGG 3 cut(s) 167, 836, 1046
MspR9I CCNGG 6 cut(s) 345, 646, 792, 836, 1070, 1157
Mva1269I GAATGC 1 cut(s) 753
MvaI CCWGG 5 cut(s) 345, 646, 792, 1070, 1157
MwoI GCNNNNNNNGC 5 cut(s) 169, 486, 704, 1157, 1179
NciI CCSGG 1 cut(s) 836
NcoI CCATGG 1 cut(s) 1125
NdeII GATC 3 cut(s) 86, 560, 866
NlaIII CATG 7 cut(s) 163, 219, 280, 830, 931, 1079, 1129
NlaIV GGNNCC 4 cut(s) 619, 730, 804, 951
NmuCI GTSAC 1 cut(s) 625
NsbI TGCGCA 1 cut(s) 600
NspI RCATGY 2 cut(s) 163, 931
PaeI GCATGC 1 cut(s) 931
PagI TCATGA 1 cut(s) 276
PctI GAATGC 1 cut(s) 753
PfeI GAWTC 2 cut(s) 19, 764
PkrI GCNGC 6 cut(s) 165, 342, 463, 833, 1063, 1181
PleI GAGTC 1 cut(s) 916
PpsI GAGTC 1 cut(s) 916
PshBI ATTAAT 1 cut(s) 129
Psp6I CCWGG 5 cut(s) 343, 644, 790, 1068, 1155
PspGI CCWGG 5 cut(s) 343, 644, 790, 1068, 1155
PspN4I GGNNCC 4 cut(s) 619, 730, 804, 951
PspPI GGNCC 5 cut(s) 347, 697, 737, 949, 1066
PstI CTGCAG 1 cut(s) 369
PvuII CAGCTG 1 cut(s) 1033
RseI CAYNNNNRTG 1 cut(s) 1080
SaqAI TTAA 4 cut(s) 9, 72, 129, 422
SatI GCNGC 6 cut(s) 164, 341, 462, 832, 1062, 1180
Sau3AI GATC 3 cut(s) 86, 560, 866
Sau96I GGNCC 5 cut(s) 347, 697, 737, 949, 1066
SchI GAGTC 1 cut(s) 917
ScrFI CCNGG 6 cut(s) 345, 646, 792, 836, 1070, 1157
SduI GDGCHC 1 cut(s) 578
SfaNI GCATC 1 cut(s) 586
SfcI CTRYAG 1 cut(s) 365
SinI GGWCC 2 cut(s) 347, 737
SmiMI CAYNNNNRTG 1 cut(s) 1080
SphI GCATGC 1 cut(s) 931
Sse9I AATT 7 cut(s) 74, 262, 565, 581, 818, 889, 1024
SsiI CCGC 5 cut(s) 100, 164, 461, 880, 1064
SspMI CTAG 3 cut(s) 905, 932, 954
StyD4I CCNGG 6 cut(s) 343, 644, 790, 834, 1068, 1155
StyI CCWWGG 3 cut(s) 350, 953, 1125
TaaI ACNGT 3 cut(s) 539, 614, 1009
TaiI ACGT 2 cut(s) 186, 723
TaqI TCGA 3 cut(s) 53, 89, 1185
TasI AATT 7 cut(s) 74, 262, 565, 581, 818, 889, 1024
TauI GCSGC 2 cut(s) 166, 464
TfiI GAWTC 2 cut(s) 19, 764
Tru1I TTAA 4 cut(s) 9, 72, 129, 422
Tru9I TTAA 4 cut(s) 9, 72, 129, 422
TscAI CASTG 5 cut(s) 374, 544, 619, 758, 980
TseFI GTSAC 1 cut(s) 625
TseI GCWGC 4 cut(s) 340, 831, 1061, 1179
Tsp45I GTSAC 1 cut(s) 625
TspDTI ATGAA 4 cut(s) 44, 293, 936, 1092
TspRI CASTG 5 cut(s) 374, 544, 619, 758, 980
VpaK11BI GGWCC 2 cut(s) 347, 737
VspI ATTAAT 1 cut(s) 129
XagI CCTNNNNNAGG 1 cut(s) 121
XapI RAATTY 6 cut(s) 74, 565, 581, 818, 889, 1024
XceI RCATGY 2 cut(s) 163, 931
XmaJI CCTAGG 1 cut(s) 953
XmiI GTMKAC 1 cut(s) 475
XspI CTAG 3 cut(s) 905, 932, 954
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.