pycom03g22080

Tubby-like F-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0001645
Physical Location & Seq
Forward (+)
22270788 .. 22273112
2325 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g22080.5

Sequence Viewer

Length: 1206 bp
ATGTCTTTTAAGAGTATGATTCAAGATATGAAAGGTGAGTTGGGGAGCATTTCTAGGAAAAGCTTTGATGTTAAATTTGGAATGAGATCGAGGTCTCAGCGGGTCGTTCAGGATACCTCATTTCGTGTTGATGCATTGAAGCAGAGTTGTTGGGCGAACATGCCTCCGGAGCTTTTGAGAGATGTGTTGATGAGAATAGAGGCGTCTGATGATGCATGGCCTCCAAGGAAAAATGTGGTGTGTTGTGCTGGTGTTTGCAGGAATTGGAGAGAAATCATGAAGGAGATTGTGAAATCCCCAGAAGTTTCGGGCAAGCTGACATTCCCGATCTCTTTGAAGCAGTCTGGTCCAAGGAACTCCCTTCTGCAGTGTTATATAAAGCGGAGTCGTAGCAACCAGACATATTATCTTTTTCTGGGTTTAAGTCAAGCCTCAACTGATGATGGGAAGTTCCTTCTTGCGGCAAAAAAGTGTAGACGCCCAACCTGCACAGACTACATCATCTCTTTGAATGCCGAGGATGTGTCAAAAGGGAACAGTGCCTTTGTTGGAAAACTCAGATCAAATTTTCTGGGCACTAAATTCACAATCTATGATGCGCAACCTACTAATAGTGGAGCCAAAGTCACTAAATGTCGTTCAACCAGGCTTGTGAATAAAGTCTCTCCCAGAGTTCCTGCTGGCAACTATCCTGTGGCCCACATTGCTTATGAGTTGAACGTCATGGGTGCCAGAGGTCCGAGGAGAATGCATTGTGTAATGGATTCCATCCCTGCCCGTGCTGTTGAGCCAGGAGGCGTGGCACCCACACAGACTGAATTTCTGCATAGCAACCTGGATAGTTTTCCATCCGTCCCTTTTTTCAGATCAACATCAACCCGGACAGAGAATTTCCCATCTCCACTAGAGTCTGGTGAGAATGAAGGCATGCTGGCATTGAGAAACAAGGCGCCTAGGTGGCACGAACAGCTCCAGTGCTGGTGTCTCAACTTCAACGGACGAGTAACAGTTGCCTCAGTAAAAAATTTTCAGCTGGTTTCTTCTCCGGAGAATGGAGTTGCGGGCCAGGAGCATGAAAATGTGATTCTTCAGTTTGGAAAAGTGGGTAAGGATGTATTCACCATGGATTATCAGTATCCAATCTCAGCCTTTCAGGCATTTGCCATTTGCCTTAGCAGCTTTGACACCAAGATTGCTTGCGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005543 GO:0008289 GO:0035091 GO:0043167 GO:0043168
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box Tub

Protein Analysis

402

Amino Acids

44.81

Weight (kDa)

9.39

Isoelectric Point (pI)

55.69

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 600
Acc36I ACCTGC 1 cut(s) 494
AccB1I GGYRCC 3 cut(s) 728, 802, 949
AccI GTMKAC 1 cut(s) 475
AccIII TCCGGA 2 cut(s) 166, 1045
AciI CCGC 4 cut(s) 100, 382, 461, 1061
AcsI RAATTY 6 cut(s) 74, 565, 581, 818, 889, 1024
AcuI CTGAAG 1 cut(s) 1073
AcyI GRCGYC 3 cut(s) 203, 478, 950
AfiI CCNNNNNNNGG 2 cut(s) 460, 1052
AgsI TTSAA 7 cut(s) 23, 139, 337, 511, 642, 718, 994
AjnI CCWGG 4 cut(s) 644, 790, 834, 1065
AjuI GAANNNNNNNTTGG 2 cut(s) 23, 55
AluBI AGCT 6 cut(s) 63, 172, 316, 970, 1033, 1179
AluI AGCT 6 cut(s) 63, 172, 316, 970, 1033, 1179
Alw26I GTCTC 3 cut(s) 99, 667, 989
Aor13HI TCCGGA 2 cut(s) 166, 1045
AoxI GGCC 3 cut(s) 218, 696, 1063
ApeKI GCWGC 1 cut(s) 1176
ApoI RAATTY 6 cut(s) 74, 565, 581, 818, 889, 1024
AspA2I CCTAGG 1 cut(s) 953
AspLEI GCGC 2 cut(s) 601, 952
AspS9I GGNCC 4 cut(s) 347, 697, 737, 1063
AsuC2I CCSGG 1 cut(s) 880
AsuHPI GGTGA 3 cut(s) 47, 926, 1111
AvaII GGWCC 2 cut(s) 347, 737
AvrII CCTAGG 1 cut(s) 953
BaeGI GKGCMC 1 cut(s) 578
BanI GGYRCC 3 cut(s) 728, 802, 949
BbvI GCAGC 1 cut(s) 1188
BccI CCATC 4 cut(s) 437, 776, 856, 904
BcgI CGANNNNNNTGC 2 cut(s) 730, 764
BciT130I CCWGG 4 cut(s) 646, 792, 836, 1067
BciVI GTATCC 2 cut(s) 106, 1146
BcnI CCSGG 1 cut(s) 880
BcoDI GTCTC 3 cut(s) 99, 667, 989
BfaI CTAG 3 cut(s) 54, 905, 954
BfmI CTRYAG 1 cut(s) 365
BfoI RGCGCY 1 cut(s) 953
BfuAI ACCTGC 1 cut(s) 494
BfuI GTATCC 2 cut(s) 106, 1146
BglI GCCNNNNNGGC 2 cut(s) 958, 1154
BisI GCNGC 2 cut(s) 462, 1177
BlnI CCTAGG 1 cut(s) 953
BlsI GCNGC 2 cut(s) 463, 1178
Bme1390I CCNGG 5 cut(s) 646, 792, 836, 880, 1067
Bme18I GGWCC 2 cut(s) 347, 737
BmgT120I GGNCC 4 cut(s) 347, 697, 737, 1063
BmiI GGNNCC 4 cut(s) 619, 730, 804, 951
BmrFI CCNGG 5 cut(s) 646, 792, 836, 880, 1067
BmsI GCATC 3 cut(s) 121, 202, 586
BpmI CTGGAG 1 cut(s) 956
Bpu10I CCTNAGC 1 cut(s) 1172
BpuMI CCSGG 1 cut(s) 880
BsaBI GATNNNNATC 1 cut(s) 871
BsaHI GRCGYC 3 cut(s) 203, 478, 950
BsaI GGTCTC 1 cut(s) 99
BsaJI CCNNGG 6 cut(s) 224, 350, 516, 740, 953, 1122
BsaWI WCCGGW 2 cut(s) 166, 1045
BsaXI ACNNNNNCTCC 2 cut(s) 609, 639
Bsc4I CCNNNNNNNGG 2 cut(s) 460, 1052
Bse1I ACTGG 1 cut(s) 973
Bse3DI GCAATG 1 cut(s) 702
Bse8I GATNNNNATC 1 cut(s) 871
BseAI TCCGGA 2 cut(s) 166, 1045
BseBI CCWGG 4 cut(s) 646, 792, 836, 1067
BseDI CCNNGG 6 cut(s) 224, 350, 516, 740, 953, 1122
BseGI GGATG 4 cut(s) 526, 768, 848, 1117
BseJI GATNNNNATC 1 cut(s) 871
BseLI CCNNNNNNNGG 2 cut(s) 460, 1052
BseMI GCAATG 1 cut(s) 702
BseMII CTCAG 4 cut(s) 110, 571, 1029, 1158
BseNI ACTGG 1 cut(s) 973
BseRI GAGGAG 1 cut(s) 757
BseSI GKGCMC 1 cut(s) 578
BseXI GCAGC 1 cut(s) 1188
BsgI GTGCAG 1 cut(s) 472
BshFI GGCC 3 cut(s) 220, 698, 1065
BshNI GGYRCC 3 cut(s) 728, 802, 949
BsiSI CCGG 3 cut(s) 167, 880, 1046
BslFI GGGAC 1 cut(s) 839
BslI CCNNNNNNNGG 2 cut(s) 460, 1052
BsmAI GTCTC 3 cut(s) 99, 667, 989
BsmFI GGGAC 1 cut(s) 839
BsmI GAATGC 2 cut(s) 517, 753
BsnI GGCC 3 cut(s) 220, 698, 1065
Bso31I GGTCTC 1 cut(s) 99
Bsp1286I GDGCHC 1 cut(s) 578
Bsp13I TCCGGA 2 cut(s) 166, 1045
Bsp143I GATC 4 cut(s) 86, 327, 560, 866
Bsp19I CCATGG 1 cut(s) 1122
BspACI CCGC 4 cut(s) 100, 382, 461, 1061
BspANI GGCC 3 cut(s) 220, 698, 1065
BspCNI CTCAG 4 cut(s) 109, 570, 1028, 1157
BspEI TCCGGA 2 cut(s) 166, 1045
BspHI TCATGA 1 cut(s) 276
BspLI GGNNCC 4 cut(s) 619, 730, 804, 951
BspMAI CTGCAG 1 cut(s) 369
BspMI ACCTGC 1 cut(s) 494
BspT107I GGYRCC 3 cut(s) 728, 802, 949
BspTNI GGTCTC 1 cut(s) 99
BsrDI GCAATG 1 cut(s) 702
BsrI ACTGG 1 cut(s) 973
BssECI CCNNGG 6 cut(s) 224, 350, 516, 740, 953, 1122
BssMI GATC 4 cut(s) 86, 327, 560, 866
BssNI GRCGYC 3 cut(s) 203, 478, 950
BssT1I CCWWGG 4 cut(s) 224, 350, 953, 1122
Bst2UI CCWGG 4 cut(s) 646, 792, 836, 1067
Bst4CI ACNGT 2 cut(s) 539, 1009
BstACI GRCGYC 3 cut(s) 203, 478, 950
BstC8I GCNNGC 6 cut(s) 314, 682, 929, 933, 1063, 1198
BstDEI CTNAG 5 cut(s) 96, 557, 1015, 1144, 1172
BstDSI CCRYGG 1 cut(s) 1122
BstF5I GGATG 4 cut(s) 526, 768, 848, 1117
BstH2I RGCGCY 1 cut(s) 953
BstHHI GCGC 2 cut(s) 601, 952
BstKTI GATC 4 cut(s) 89, 330, 563, 869
BstMAI GTCTC 3 cut(s) 99, 667, 989
BstMBI GATC 4 cut(s) 86, 327, 560, 866
BstMWI GCNNNNNNNGC 7 cut(s) 169, 486, 704, 958, 967, 1154, 1176
BstNI CCWGG 4 cut(s) 646, 792, 836, 1067
BstNSI RCATGY 2 cut(s) 163, 931
BstSCI CCNGG 5 cut(s) 644, 790, 834, 878, 1065
BstSFI CTRYAG 1 cut(s) 365
BstSLI GKGCMC 1 cut(s) 578
BstV1I GCAGC 1 cut(s) 1188
BsuI GTATCC 2 cut(s) 106, 1146
BsuRI GGCC 3 cut(s) 220, 698, 1065
BtgI CCRYGG 1 cut(s) 1122
BtsCI GGATG 4 cut(s) 526, 768, 848, 1117
BtsI GCAGTG 1 cut(s) 374
BtsIMutI CAGTG 3 cut(s) 374, 544, 980
BveI ACCTGC 1 cut(s) 494
Cac8I GCNNGC 6 cut(s) 314, 682, 929, 933, 1063, 1198
CciI TCATGA 1 cut(s) 276
CfoI GCGC 2 cut(s) 601, 952
Cfr13I GGNCC 4 cut(s) 347, 697, 737, 1063
CseI GACGC 2 cut(s) 192, 486
CviAII CATG 7 cut(s) 160, 216, 277, 724, 928, 1073, 1123
DdeI CTNAG 5 cut(s) 96, 557, 1015, 1144, 1172
DinI GGCGCC 1 cut(s) 951
DpnI GATC 4 cut(s) 88, 329, 562, 868
DpnII GATC 4 cut(s) 86, 327, 560, 866
Eco130I CCWWGG 4 cut(s) 224, 350, 953, 1122
Eco31I GGTCTC 1 cut(s) 99
Eco47I GGWCC 2 cut(s) 347, 737
Eco57I CTGAAG 1 cut(s) 1073
EcoRII CCWGG 4 cut(s) 644, 790, 834, 1065
EcoT14I CCWWGG 4 cut(s) 224, 350, 953, 1122
EcoT22I ATGCAT 3 cut(s) 136, 217, 753
EgeI GGCGCC 1 cut(s) 951
EheI GGCGCC 1 cut(s) 951
ErhI CCWWGG 4 cut(s) 224, 350, 953, 1122
FaeI CATG 7 cut(s) 163, 219, 280, 727, 931, 1076, 1126
FaqI GGGAC 1 cut(s) 839
FatI CATG 7 cut(s) 159, 215, 276, 723, 927, 1072, 1122
FauI CCCGC 2 cut(s) 93, 1054
FblI GTMKAC 1 cut(s) 475
Fnu4HI GCNGC 2 cut(s) 462, 1177
FokI GGATG 4 cut(s) 533, 755, 835, 1124
Fsp4HI GCNGC 2 cut(s) 462, 1177
FspBI CTAG 3 cut(s) 54, 905, 954
FspI TGCGCA 1 cut(s) 600
GlaI GCGC 2 cut(s) 600, 951
GluI GCNGC 2 cut(s) 462, 1177
GsuI CTGGAG 1 cut(s) 956
HaeII RGCGCY 1 cut(s) 953
HaeIII GGCC 3 cut(s) 220, 698, 1065
HapII CCGG 3 cut(s) 167, 880, 1046
HgaI GACGC 2 cut(s) 192, 486
HhaI GCGC 2 cut(s) 601, 952
Hin1I GRCGYC 3 cut(s) 203, 478, 950
Hin1II CATG 7 cut(s) 163, 219, 280, 727, 931, 1076, 1126
Hin6I GCGC 2 cut(s) 599, 950
HinP1I GCGC 2 cut(s) 599, 950
HindIII AAGCTT 1 cut(s) 61
HinfI GANTC 5 cut(s) 19, 385, 764, 908, 1084
HpaII CCGG 3 cut(s) 167, 880, 1046
HphI GGTGA 3 cut(s) 47, 926, 1111
Hpy166II GTNNAC 1 cut(s) 476
Hpy188I TCNGA 4 cut(s) 208, 560, 741, 866
Hpy188III TCNNGA 6 cut(s) 23, 110, 167, 277, 325, 1046
Hpy8I GTNNAC 1 cut(s) 476
HpyAV CCTTC 4 cut(s) 274, 371, 464, 917
HpyCH4III ACNGT 2 cut(s) 539, 1009
HpyCH4IV ACGT 1 cut(s) 720
HpyCH4V TGCA 7 cut(s) 134, 215, 258, 367, 489, 751, 826
HpyF10VI GCNNNNNNNGC 7 cut(s) 169, 486, 704, 958, 967, 1154, 1176
HpyF3I CTNAG 5 cut(s) 96, 557, 1015, 1144, 1172
HpySE526I ACGT 1 cut(s) 720
Hsp92I GRCGYC 3 cut(s) 203, 478, 950
Hsp92II CATG 7 cut(s) 163, 219, 280, 727, 931, 1076, 1126
HspAI GCGC 2 cut(s) 599, 950
KasI GGCGCC 1 cut(s) 949
Kpn2I TCCGGA 2 cut(s) 166, 1045
Kzo9I GATC 4 cut(s) 86, 327, 560, 866
LmnI GCTCC 5 cut(s) 45, 169, 617, 975, 1069
Lsp1109I GCAGC 1 cut(s) 1188
LweI GCATC 3 cut(s) 121, 202, 586
MaeI CTAG 3 cut(s) 54, 905, 954
MaeII ACGT 1 cut(s) 720
MaeIII GTNAC 2 cut(s) 625, 1003
MalI GATC 4 cut(s) 88, 329, 562, 868
MboI GATC 4 cut(s) 86, 327, 560, 866
MboII GAAGA 2 cut(s) 1032, 1079
MhlI GDGCHC 1 cut(s) 578
MluCI AATT 7 cut(s) 74, 262, 565, 581, 818, 889, 1024
Mly113I GGCGCC 1 cut(s) 950
MlyI GAGTC 2 cut(s) 394, 917
MmeI TCCRAC 1 cut(s) 529
Mph1103I ATGCAT 3 cut(s) 136, 217, 753
MroI TCCGGA 2 cut(s) 166, 1045
MseI TTAA 3 cut(s) 9, 72, 422
MslI CAYNNNNRTG 1 cut(s) 1077
MspA1I CMGCKG 2 cut(s) 100, 1033
MspI CCGG 3 cut(s) 167, 880, 1046
MspR9I CCNGG 5 cut(s) 646, 792, 836, 880, 1067
Mva1269I GAATGC 2 cut(s) 517, 753
MvaI CCWGG 4 cut(s) 646, 792, 836, 1067
MwoI GCNNNNNNNGC 7 cut(s) 169, 486, 704, 958, 967, 1154, 1176
NarI GGCGCC 1 cut(s) 950
NciI CCSGG 1 cut(s) 880
NcoI CCATGG 1 cut(s) 1122
NdeII GATC 4 cut(s) 86, 327, 560, 866
NlaIII CATG 7 cut(s) 163, 219, 280, 727, 931, 1076, 1126
NlaIV GGNNCC 4 cut(s) 619, 730, 804, 951
NmeAIII GCCGAG 1 cut(s) 541
NmuCI GTSAC 1 cut(s) 625
NsbI TGCGCA 1 cut(s) 600
NsiI ATGCAT 3 cut(s) 136, 217, 753
NspI RCATGY 2 cut(s) 163, 931
PaeI GCATGC 1 cut(s) 931
PagI TCATGA 1 cut(s) 276
PctI GAATGC 2 cut(s) 517, 753
PfeI GAWTC 3 cut(s) 19, 764, 1084
PkrI GCNGC 2 cut(s) 463, 1178
PleI GAGTC 2 cut(s) 393, 916
PluTI GGCGCC 1 cut(s) 953
PpsI GAGTC 2 cut(s) 393, 916
Psp6I CCWGG 4 cut(s) 644, 790, 834, 1065
PspGI CCWGG 4 cut(s) 644, 790, 834, 1065
PspN4I GGNNCC 4 cut(s) 619, 730, 804, 951
PspPI GGNCC 4 cut(s) 347, 697, 737, 1063
PstI CTGCAG 1 cut(s) 369
PvuII CAGCTG 1 cut(s) 1033
RseI CAYNNNNRTG 1 cut(s) 1077
SaqAI TTAA 3 cut(s) 9, 72, 422
SatI GCNGC 2 cut(s) 462, 1177
Sau3AI GATC 4 cut(s) 86, 327, 560, 866
Sau96I GGNCC 4 cut(s) 347, 697, 737, 1063
SchI GAGTC 2 cut(s) 394, 917
ScrFI CCNGG 5 cut(s) 646, 792, 836, 880, 1067
SduI GDGCHC 1 cut(s) 578
SfaNI GCATC 3 cut(s) 121, 202, 586
SfcI CTRYAG 1 cut(s) 365
SfoI GGCGCC 1 cut(s) 951
SinI GGWCC 2 cut(s) 347, 737
SmiMI CAYNNNNRTG 1 cut(s) 1077
SphI GCATGC 1 cut(s) 931
Sse9I AATT 7 cut(s) 74, 262, 565, 581, 818, 889, 1024
SsiI CCGC 4 cut(s) 100, 382, 461, 1061
SspDI GGCGCC 1 cut(s) 949
SspMI CTAG 3 cut(s) 54, 905, 954
StyD4I CCNGG 5 cut(s) 644, 790, 834, 878, 1065
StyI CCWWGG 4 cut(s) 224, 350, 953, 1122
TaaI ACNGT 2 cut(s) 539, 1009
TaiI ACGT 1 cut(s) 723
TaqI TCGA 1 cut(s) 89
TasI AATT 7 cut(s) 74, 262, 565, 581, 818, 889, 1024
TauI GCSGC 1 cut(s) 464
TfiI GAWTC 3 cut(s) 19, 764, 1084
Tru1I TTAA 3 cut(s) 9, 72, 422
Tru9I TTAA 3 cut(s) 9, 72, 422
TscAI CASTG 3 cut(s) 374, 544, 980
TseFI GTSAC 1 cut(s) 625
TseI GCWGC 1 cut(s) 1176
Tsp45I GTSAC 1 cut(s) 625
TspDTI ATGAA 4 cut(s) 44, 293, 936, 1089
TspGWI ACGGA 2 cut(s) 841, 1011
TspRI CASTG 3 cut(s) 374, 544, 980
VpaK11BI GGWCC 2 cut(s) 347, 737
XapI RAATTY 6 cut(s) 74, 565, 581, 818, 889, 1024
XceI RCATGY 2 cut(s) 163, 931
XmaJI CCTAGG 1 cut(s) 953
XmiI GTMKAC 1 cut(s) 475
XspI CTAG 3 cut(s) 54, 905, 954
Zsp2I ATGCAT 3 cut(s) 136, 217, 753
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.