FvH4_2g33850

Receptor protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0004846
Physical Location & Seq
Reverse (-)
25277491 .. 25280177
2687 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g33850.t1

Sequence Viewer

Length: 2268 bp
ATGTCGCCGGACGCCGTATTTACCGCTGGCTTTTTTCCGGTCGGCGAAAACGCGTTTTGCTTTGGAATATGGTTCTCGGAGCCAACAACCATTACAGACAACCTTACCGTGGTGTGGATAGCTAATCGTGACGAGCCGGTAAATGGTCAACGCTCCAAGCTCACCCTGCACAAAAATGGAAACCTTATTCTCTCCGACATCGACCGATACACCGTGTGGTCCTCCGACACAGCTTCAAACTCATCGGCCCACTTGCACCTCAGAAACACCGGCAATCTAGTTCTTCTCGAAGAGCACGGCGTCGTGTTGTGGGAAAGCTTTGCTTCGCCGACGGACACGCTTTTGCCTCAGCAACCGCTCACAAGAAGCACACAGCTCATTTCTTCAAGAAGCCTCACCAACTATTCCTCCGGCTTCTACTCGTTTTACTTCGACAACGACAACACTCTTCGTATACTCTACAATGGCCCCGAGGTTTCGAGCATTTACTGGCCCGATCCATGGCTTCTGAGTTATCAAGCAGGAAGATCCACATACAACAACACTCGAATTGCAGTTTTTGACAGCTTGGGAAATTTTAGCTCATCTGATAATTTTACCGTTTGGACAATCGACTACGGTGCGAAACTGCAGCGAATATTCAGGATGGATGTAGATGGAAATCTCCGAGGCTACAGTCGAAAAGGTCAGGGAGATAAATGGATTGTTACATGGGAAGCAGTTTTACAACCATGTAAAATCCATGGCATTTGCGGAGCTAATAGCTACTGCAGCTATATTCCTAGTACAGGTAGGAAATGTTCGTGTCTGCCGGGTTATGAAATGGTCAGCAAAACCGATTGGTCTTATGGGTGTAAACCGGCTGGTTTCAAGCTGTCCTGCAAGAGTAGTGCAGAGGAAGAGTTCGGTTTTGTGCATCTTTCACGTATTGAAATGTATGGGTATGATTATGCCGTCTATGGCAATTACACATTAGATGAGTGCAAAAGTGTGTGCCTGGACTTGTGCAACTGTACTGGATTCCACCACAAATTTGGTGGCGGTCAGTATCAGTGTTATCCCAAGTATCAAATGCGAAATGGCTACCGCTCGCCTGCTTTCGATGGGGAGCTGTATGTGAAGCTACCAAAAGGCCTCCTCAATGCTACTTATAAGGATTCCAAAATGTGCTCAGCTAAACTTACAGCACAACTTGACAGAACATATGAAAGAAGCAATCCCAGTGGATCGATCCAGTTTATGCTTAAATTCGCATCGGCCGTGGGAGGACTAGAGGTCATTTGCATCTTTGTTGTGTGGTTTTTTGTAACGAGAAGAAGCAGCGGGGATAAAAAAGATTCAGAATTAGAAGCACTTGCTCAAGGCTACCTTCAAACTGCCAGTGGATTTAGAAGGTTTACATACTCCGAATTGAAGAAGGCCACAAAAGGATTCAAGGAAGAGATTGGAAAAGGAGGAGGAGGAGTTGTGTACAAAGGCGTGTTGTCCGATCAGCGAGTTGCAGCGATTAAGCAACTGAATGATTGTAATCAAGGAGAAGCTGAGTTCCTTGCAGAAGTAAGCCTTCTTGGTAAGCTTTACCATATGAACTTGATAGAGATGTGGGGTTATTGCGTGGAAGACAAGCATAGGCTTCTCGTGTATGAGTACATGGAGCATGGTTCCTTGGCAAAAGTTTTATCTTCCAAAGCGCTTGATTGGAAGAAGAGGTATGATATTGCTCTTGGCACAGCAAAAGGACTGGCTTATCTTCATGAAGAGTGCTTGGAATGGGTTCTACATTGTGATGTGAAGCCTCAGAACATACTCTTGGACTCCTATTACAATCCCAAGGTAGCAGATTTTGGGTTGTCCAAGCTATTAAACAGAAGCGAGAACACCAGCTCAGACTTCTCGAGGATTAGAGGAACCAGAGGTTACATGGCTCCAGAGTGGGTGCACAATATGCCCATCACTTCGAAGGTGGATGTCTACAGTTATGGAATTGTTGTGCTAGAAATGCTCACCGGGAAGAACCCAACTGAAAGCAATCTTCAATCGGTTGAAGGTGGAGGAGAGACACACAAGAAGATGATGGCACAATGGGTGATGGAAAAAATGAATGGAGCATTAGCAAATGCAGAGACCAAAATGGGAGGTGAAAATGAAAAGGGTAAGCTGGAAATTCTTATTAAAGTGGCGCTCCAGTGTATAGAGGAAGACAGAAATGCAAGACCCTCTATGAGTCGAGTAGTTGAGATGCTTCTACATCATCAATTTCAGGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0016020
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin PAN_1 PAN_2 Pkinase S_locus_glycop

Protein Analysis

756

Amino Acids

85.01

Weight (kDa)

6.97

Isoelectric Point (pI)

39.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 36 - 132 1e-23 D-mannose binding lectin
S_locus_glycop PF00954 208 - 275 1e-07 S-locus glycoprotein domain
PK_Tyr_Ser-Thr PF07714 478 - 748 7.6e-44 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 479 - 746 5e-54 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1152
AccBSI CCGCTC 2 cut(s) 358, 1089
AccI GTMKAC 2 cut(s) 454, 1971
AccII CGCG 1 cut(s) 53
AciI CCGC 6 cut(s) 24, 356, 753, 1041, 1087, 1323
AclWI GGATC 4 cut(s) 491, 522, 1225, 1234
AcoI YGGCCR 1 cut(s) 1257
AcsI RAATTY 4 cut(s) 574, 1031, 1247, 2163
AcyI GRCGYC 2 cut(s) 12, 300
AdeI CACNNNGTG 1 cut(s) 216
AfaI GTAC 4 cut(s) 787, 1015, 1472, 1649
AfeI AGCGCT 1 cut(s) 1692
AfiI CCNNNNNNNGG 5 cut(s) 109, 114, 143, 501, 788
AflIII ACRYGT 1 cut(s) 51
AgsI TTSAA 9 cut(s) 237, 387, 871, 932, 1373, 1414, 1435, 2036, 2045
AhdI GACNNNNNGTC 1 cut(s) 1274
AjnI CCWGG 1 cut(s) 996
AjuI GAANNNNNNNTTGG 2 cut(s) 1793, 1825
Alw21I GWGCWC 3 cut(s) 297, 1172, 1941
Alw26I GTCTC 2 cut(s) 2051, 2117
Alw44I GTGCAC 1 cut(s) 1937
AlwI GGATC 4 cut(s) 491, 522, 1225, 1234
Ama87I CYCGRG 2 cut(s) 470, 1894
Aor51HI AGCGCT 1 cut(s) 1692
AoxI GGCC 6 cut(s) 246, 466, 491, 1132, 1257, 1419
ApaLI GTGCAC 1 cut(s) 1937
ApeKI GCWGC 4 cut(s) 631, 771, 1320, 1502
ApoI RAATTY 4 cut(s) 574, 1031, 1247, 2163
Asp700I GAANNNNTTC 1 cut(s) 1773
AspLEI GCGC 2 cut(s) 1693, 2182
AspS9I GGNCC 4 cut(s) 219, 247, 467, 492
AsuC2I CCSGG 2 cut(s) 813, 2008
AsuHPI GGTGA 5 cut(s) 154, 388, 1996, 2098, 2150
AsuII TTCGAA 1 cut(s) 1958
AvaI CYCGRG 2 cut(s) 470, 1894
AvaII GGWCC 1 cut(s) 219
BaeGI GKGCMC 1 cut(s) 1941
BarI GAAGNNNNNNTAC 2 cut(s) 708, 740
BauI CACGAG 1 cut(s) 1637
BbsI GAAGAC 2 cut(s) 1626, 2205
Bbv12I GWGCWC 3 cut(s) 297, 1172, 1941
BbvCI CCTCAGC 1 cut(s) 348
BbvI GCAGC 4 cut(s) 643, 783, 1332, 1514
BccI CCATC 6 cut(s) 640, 650, 1097, 1958, 2068, 2083
BceAI ACGGC 3 cut(s) 313, 938, 1244
BcgI CGANNNNNNTGC 2 cut(s) 602, 636
BciT130I CCWGG 1 cut(s) 998
BcnI CCSGG 2 cut(s) 813, 2008
BcoDI GTCTC 2 cut(s) 2051, 2117
BfaI CTAG 4 cut(s) 278, 783, 1271, 1994
BfmI CTRYAG 4 cut(s) 629, 673, 769, 1972
BfoI RGCGCY 2 cut(s) 1694, 2183
BisI GCNGC 4 cut(s) 632, 772, 1321, 1503
BlpI GCTNAGC 1 cut(s) 1171
BlsI GCNGC 4 cut(s) 633, 773, 1322, 1504
Bme1390I CCNGG 3 cut(s) 813, 998, 2008
Bme18I GGWCC 1 cut(s) 219
BmeRI GACNNNNNGTC 1 cut(s) 1274
BmeT110I CYCGRG 2 cut(s) 470, 1894
BmgT120I GGNCC 4 cut(s) 219, 247, 467, 492
BmiI GGNNCC 5 cut(s) 81, 469, 1663, 1909, 1926
BmrFI CCNGG 3 cut(s) 813, 998, 2008
BmrI ACTGGG 1 cut(s) 1215
BmsI GCATC 4 cut(s) 925, 1262, 1293, 2229
BmuI ACTGGG 1 cut(s) 1215
BpiI GAAGAC 2 cut(s) 1626, 2205
BpmI CTGGAG 2 cut(s) 1911, 2168
Bpu10I CCTNAGC 1 cut(s) 348
Bpu1102I GCTNAGC 1 cut(s) 1171
Bpu14I TTCGAA 1 cut(s) 1958
BpuEI CTTGAG 1 cut(s) 1344
BpuMI CCSGG 2 cut(s) 813, 2008
Bsa29I ATCGAT 1 cut(s) 1229
BsaAI YACGTR 1 cut(s) 926
BsaBI GATNNNNATC 2 cut(s) 660, 1527
BsaHI GRCGYC 2 cut(s) 12, 300
BsaI GGTCTC 1 cut(s) 2117
BsaJI CCNNGG 8 cut(s) 108, 471, 500, 667, 742, 1260, 1665, 1830
BsaWI WCCGGW 1 cut(s) 37
BsaXI ACNNNNNCTCC 6 cut(s) 392, 422, 1449, 1452, 1479, 1482
Bsc4I CCNNNNNNNGG 5 cut(s) 109, 114, 143, 501, 788
Bse118I RCCGGY 3 cut(s) 136, 269, 859
Bse1I ACTGG 7 cut(s) 494, 1021, 1221, 1234, 1380, 1746, 2185
Bse8I GATNNNNATC 2 cut(s) 660, 1527
BseBI CCWGG 1 cut(s) 998
BseCI ATCGAT 1 cut(s) 1229
BseDI CCNNGG 8 cut(s) 108, 471, 500, 667, 742, 1260, 1665, 1830
BseGI GGATG 3 cut(s) 651, 655, 1972
BseJI GATNNNNATC 2 cut(s) 660, 1527
BseLI CCNNNNNNNGG 5 cut(s) 109, 114, 143, 501, 788
BseMII CTCAG 7 cut(s) 274, 362, 500, 1185, 1533, 1811, 1899
BseNI ACTGG 7 cut(s) 494, 1021, 1221, 1234, 1380, 1746, 2185
BseRI GAGGAG 5 cut(s) 1127, 1470, 1473, 1476, 2067
BseSI GKGCMC 1 cut(s) 1941
BseX3I CGGCCG 1 cut(s) 1257
BseXI GCAGC 4 cut(s) 643, 783, 1332, 1514
BsgI GTGCAG 2 cut(s) 152, 912
Bsh1236I CGCG 1 cut(s) 53
Bsh1285I CGRYCG 3 cut(s) 42, 205, 1260
BshFI GGCC 6 cut(s) 248, 468, 493, 1134, 1259, 1421
BshVI ATCGAT 1 cut(s) 1229
BsiEI CGRYCG 3 cut(s) 42, 205, 1260
BsiHKAI GWGCWC 3 cut(s) 297, 1172, 1941
BsiHKCI CYCGRG 2 cut(s) 470, 1894
BsiSI CCGG 8 cut(s) 8, 38, 137, 270, 411, 812, 860, 2007
BslI CCNNNNNNNGG 5 cut(s) 109, 114, 143, 501, 788
BsmAI GTCTC 2 cut(s) 2051, 2117
BsnI GGCC 6 cut(s) 248, 468, 493, 1134, 1259, 1421
Bso31I GGTCTC 1 cut(s) 2117
BsoBI CYCGRG 2 cut(s) 470, 1894
Bsp119I TTCGAA 1 cut(s) 1958
Bsp1286I GDGCHC 3 cut(s) 297, 1172, 1941
Bsp1407I TGTACA 1 cut(s) 1470
Bsp143I GATC 5 cut(s) 496, 527, 1226, 1230, 1489
Bsp1720I GCTNAGC 1 cut(s) 1171
Bsp19I CCATGG 2 cut(s) 500, 742
BspACI CCGC 6 cut(s) 24, 356, 753, 1041, 1087, 1323
BspANI GGCC 6 cut(s) 248, 468, 493, 1134, 1259, 1421
BspCNI CTCAG 7 cut(s) 273, 361, 501, 1184, 1534, 1810, 1898
BspDI ATCGAT 1 cut(s) 1229
BspFNI CGCG 1 cut(s) 53
BspHI TCATGA 1 cut(s) 1753
BspLI GGNNCC 5 cut(s) 81, 469, 1663, 1909, 1926
BspMAI CTGCAG 2 cut(s) 633, 773
BspPI GGATC 4 cut(s) 491, 522, 1225, 1234
BspQI GCTCTTC 1 cut(s) 285
BspT104I TTCGAA 1 cut(s) 1958
BspTNI GGTCTC 1 cut(s) 2117
BsrBI CCGCTC 2 cut(s) 358, 1089
BsrFI RCCGGY 3 cut(s) 136, 269, 859
BsrGI TGTACA 1 cut(s) 1470
BsrI ACTGG 7 cut(s) 494, 1021, 1221, 1234, 1380, 1746, 2185
BssAI RCCGGY 3 cut(s) 136, 269, 859
BssECI CCNNGG 8 cut(s) 108, 471, 500, 667, 742, 1260, 1665, 1830
BssMI GATC 5 cut(s) 496, 527, 1226, 1230, 1489
BssNAI GTATAC 1 cut(s) 455
BssNI GRCGYC 2 cut(s) 12, 300
BssSI CACGAG 1 cut(s) 1637
BssT1I CCWWGG 4 cut(s) 500, 742, 1665, 1830
Bst1107I GTATAC 1 cut(s) 455
Bst2BI CACGAG 1 cut(s) 1637
Bst2UI CCWGG 1 cut(s) 998
Bst4CI ACNGT 7 cut(s) 109, 214, 601, 620, 677, 1013, 1976
Bst6I CTCTTC 6 cut(s) 285, 453, 894, 1434, 1700, 1752
BstACI GRCGYC 2 cut(s) 12, 300
BstAPI GCANNNNNTGC 1 cut(s) 1945
BstAUI TGTACA 1 cut(s) 1470
BstBAI YACGTR 1 cut(s) 926
BstBI TTCGAA 1 cut(s) 1958
BstC8I GCNNGC 3 cut(s) 28, 1091, 1095
BstDEI CTNAG 7 cut(s) 260, 348, 509, 1171, 1542, 1797, 1885
BstDSI CCRYGG 4 cut(s) 108, 500, 742, 1260
BstENI CCTNNNNNAGG 1 cut(s) 786
BstF5I GGATG 3 cut(s) 651, 655, 1972
BstFNI CGCG 1 cut(s) 53
BstH2I RGCGCY 2 cut(s) 1694, 2183
BstHHI GCGC 2 cut(s) 1693, 2182
BstKTI GATC 5 cut(s) 499, 530, 1229, 1233, 1492
BstMAI GTCTC 2 cut(s) 2051, 2117
BstMBI GATC 5 cut(s) 496, 527, 1226, 1230, 1489
BstMCI CGRYCG 3 cut(s) 42, 205, 1260
BstMWI GCNNNNNNNGC 4 cut(s) 166, 771, 1945, 1999
BstNI CCWGG 1 cut(s) 998
BstSCI CCNGG 3 cut(s) 811, 996, 2006
BstSFI CTRYAG 4 cut(s) 629, 673, 769, 1972
BstSLI GKGCMC 1 cut(s) 1941
BstUI CGCG 1 cut(s) 53
BstV1I GCAGC 4 cut(s) 643, 783, 1332, 1514
BstV2I GAAGAC 2 cut(s) 1626, 2205
BstX2I RGATCY 1 cut(s) 527
BstXI CCANNNNNNTGG 1 cut(s) 1034
BstYI RGATCY 1 cut(s) 527
BstZ17I GTATAC 1 cut(s) 455
BstZI CGGCCG 1 cut(s) 1257
Bsu15I ATCGAT 1 cut(s) 1229
BsuRI GGCC 6 cut(s) 248, 468, 493, 1134, 1259, 1421
BsuTUI ATCGAT 1 cut(s) 1229
BtgI CCRYGG 4 cut(s) 108, 500, 742, 1260
BtsCI GGATG 3 cut(s) 651, 655, 1972
BtsIMutI CAGTG 4 cut(s) 1058, 1228, 1387, 2192
Cac8I GCNNGC 3 cut(s) 28, 1091, 1095
CciI TCATGA 1 cut(s) 1753
CfoI GCGC 2 cut(s) 1693, 2182
Cfr10I RCCGGY 3 cut(s) 136, 269, 859
Cfr13I GGNCC 4 cut(s) 219, 247, 467, 492
ClaI ATCGAT 1 cut(s) 1229
CseI GACGC 2 cut(s) 20, 289
Csp6I GTAC 4 cut(s) 786, 1014, 1471, 1648
CspCI CAANNNNNGTGG 4 cut(s) 1018, 1053, 1204, 1239
CviAII CATG 8 cut(s) 501, 711, 732, 743, 1651, 1658, 1754, 1921
CviQI GTAC 4 cut(s) 786, 1014, 1471, 1648
DdeI CTNAG 7 cut(s) 260, 348, 509, 1171, 1542, 1797, 1885
DpnI GATC 5 cut(s) 498, 529, 1228, 1232, 1491
DpnII GATC 5 cut(s) 496, 527, 1226, 1230, 1489
DraIII CACNNNGTG 1 cut(s) 216
DriI GACNNNNNGTC 1 cut(s) 1274
EaeI YGGCCR 1 cut(s) 1257
EagI CGGCCG 1 cut(s) 1257
Eam1104I CTCTTC 6 cut(s) 285, 453, 894, 1434, 1700, 1752
Eam1105I GACNNNNNGTC 1 cut(s) 1274
EarI CTCTTC 6 cut(s) 285, 453, 894, 1434, 1700, 1752
EclXI CGGCCG 1 cut(s) 1257
Eco130I CCWWGG 4 cut(s) 500, 742, 1665, 1830
Eco147I AGGCCT 1 cut(s) 1134
Eco31I GGTCTC 1 cut(s) 2117
Eco47I GGWCC 1 cut(s) 219
Eco47III AGCGCT 1 cut(s) 1692
Eco52I CGGCCG 1 cut(s) 1257
Eco88I CYCGRG 2 cut(s) 470, 1894
EcoNI CCTNNNNNAGG 1 cut(s) 786
EcoRII CCWGG 1 cut(s) 996
EcoT14I CCWWGG 4 cut(s) 500, 742, 1665, 1830
ErhI CCWWGG 4 cut(s) 500, 742, 1665, 1830
FaeI CATG 8 cut(s) 504, 714, 735, 746, 1654, 1661, 1757, 1924
FalI AAGNNNNNCTT 6 cut(s) 307, 339, 1353, 1385, 1548, 1580
FatI CATG 8 cut(s) 500, 710, 731, 742, 1650, 1657, 1753, 1920
FauI CCCGC 1 cut(s) 1316
FauNDI CATATG 2 cut(s) 1204, 1584
FblI GTMKAC 2 cut(s) 454, 1971
Fnu4HI GCNGC 4 cut(s) 632, 772, 1321, 1503
FokI GGATG 3 cut(s) 658, 662, 1979
Fsp4HI GCNGC 4 cut(s) 632, 772, 1321, 1503
FspBI CTAG 4 cut(s) 278, 783, 1271, 1994
GlaI GCGC 2 cut(s) 1692, 2181
GluI GCNGC 4 cut(s) 632, 772, 1321, 1503
GsuI CTGGAG 2 cut(s) 1911, 2168
HaeII RGCGCY 2 cut(s) 1694, 2183
HaeIII GGCC 6 cut(s) 248, 468, 493, 1134, 1259, 1421
HapII CCGG 8 cut(s) 8, 38, 137, 270, 411, 812, 860, 2007
HgaI GACGC 2 cut(s) 20, 289
HhaI GCGC 2 cut(s) 1693, 2182
Hin1I GRCGYC 2 cut(s) 12, 300
Hin1II CATG 8 cut(s) 504, 714, 735, 746, 1654, 1661, 1757, 1924
Hin6I GCGC 2 cut(s) 1691, 2180
HinP1I GCGC 2 cut(s) 1691, 2180
HincII GTYRAC 1 cut(s) 149
HindII GTYRAC 1 cut(s) 149
HindIII AAGCTT 2 cut(s) 316, 1574
HinfI GANTC 6 cut(s) 1020, 1157, 1337, 1431, 1814, 2224
HpaII CCGG 8 cut(s) 8, 38, 137, 270, 411, 812, 860, 2007
HphI GGTGA 5 cut(s) 154, 388, 1996, 2098, 2150
Hpy166II GTNNAC 7 cut(s) 149, 455, 857, 1398, 1471, 1939, 1972
Hpy188III TCNNGA 7 cut(s) 128, 287, 387, 643, 1754, 1894, 1928
Hpy8I GTNNAC 7 cut(s) 149, 455, 857, 1398, 1471, 1939, 1972
Hpy99I CGWCG 2 cut(s) 305, 334
HpyAV CCTTC 6 cut(s) 1379, 1386, 1411, 1574, 1954, 2039
HpyCH4III ACNGT 7 cut(s) 109, 214, 601, 620, 677, 1013, 1976
HpyCH4IV ACGT 1 cut(s) 925
HpyF10VI GCNNNNNNNGC 4 cut(s) 166, 771, 1945, 1999
HpyF3I CTNAG 7 cut(s) 260, 348, 509, 1171, 1542, 1797, 1885
HpySE526I ACGT 1 cut(s) 925
Hsp92I GRCGYC 2 cut(s) 12, 300
Hsp92II CATG 8 cut(s) 504, 714, 735, 746, 1654, 1661, 1757, 1924
HspAI GCGC 2 cut(s) 1691, 2180
Kzo9I GATC 5 cut(s) 496, 527, 1226, 1230, 1489
LguI GCTCTTC 1 cut(s) 285
LmnI GCTCC 8 cut(s) 79, 158, 755, 1108, 1654, 1930, 2105, 2187
Lsp1109I GCAGC 4 cut(s) 643, 783, 1332, 1514
LweI GCATC 4 cut(s) 925, 1262, 1293, 2229
MaeI CTAG 4 cut(s) 278, 783, 1271, 1994
MaeII ACGT 1 cut(s) 925
MaeIII GTNAC 4 cut(s) 128, 706, 1306, 1916
MalI GATC 5 cut(s) 498, 529, 1228, 1232, 1491
MbiI CCGCTC 2 cut(s) 358, 1089
MboI GATC 5 cut(s) 496, 527, 1226, 1230, 1489
MflI RGATCY 1 cut(s) 527
MhlI GDGCHC 3 cut(s) 297, 1172, 1941
MluI ACGCGT 1 cut(s) 51
MlyI GAGTC 2 cut(s) 1808, 2233
MmeI TCCRAC 2 cut(s) 219, 249
MroXI GAANNNNTTC 1 cut(s) 1773
MseI TTAA 4 cut(s) 1245, 1509, 1862, 2172
MslI CAYNNNNRTG 2 cut(s) 174, 1785
MspA1I CMGCKG 2 cut(s) 26, 1323
MspI CCGG 8 cut(s) 8, 38, 137, 270, 411, 812, 860, 2007
MspR9I CCNGG 3 cut(s) 813, 998, 2008
MvaI CCWGG 1 cut(s) 998
MvnI CGCG 1 cut(s) 53
MwoI GCNNNNNNNGC 4 cut(s) 166, 771, 1945, 1999
NciI CCSGG 2 cut(s) 813, 2008
NcoI CCATGG 2 cut(s) 500, 742
NdeI CATATG 2 cut(s) 1204, 1584
NdeII GATC 5 cut(s) 496, 527, 1226, 1230, 1489
NlaIII CATG 8 cut(s) 504, 714, 735, 746, 1654, 1661, 1757, 1924
NlaIV GGNNCC 5 cut(s) 81, 469, 1663, 1909, 1926
NmuCI GTSAC 1 cut(s) 128
NspV TTCGAA 1 cut(s) 1958
PaeR7I CTCGAG 1 cut(s) 1894
PagI TCATGA 1 cut(s) 1753
PceI AGGCCT 1 cut(s) 1134
PciSI GCTCTTC 1 cut(s) 285
PcsI WCGNNNNNNNCGW 1 cut(s) 1257
PdmI GAANNNNTTC 1 cut(s) 1773
PfeI GAWTC 4 cut(s) 1020, 1157, 1337, 1431
PkrI GCNGC 4 cut(s) 633, 773, 1322, 1504
PleI GAGTC 2 cut(s) 1808, 2232
PpsI GAGTC 2 cut(s) 1808, 2232
Ppu21I YACGTR 1 cut(s) 926
PsiI TTATAA 1 cut(s) 1152
Psp6I CCWGG 1 cut(s) 996
PspGI CCWGG 1 cut(s) 996
PspN4I GGNNCC 5 cut(s) 81, 469, 1663, 1909, 1926
PspPI GGNCC 4 cut(s) 219, 247, 467, 492
PsrI GAACNNNNNNTAC 2 cut(s) 784, 816
PstI CTGCAG 2 cut(s) 633, 773
PsuI RGATCY 1 cut(s) 527
RsaI GTAC 4 cut(s) 787, 1015, 1472, 1649
RsaNI GTAC 4 cut(s) 786, 1014, 1471, 1648
RseI CAYNNNNRTG 2 cut(s) 174, 1785
SapI GCTCTTC 1 cut(s) 285
SaqAI TTAA 4 cut(s) 1245, 1509, 1862, 2172
SatI GCNGC 4 cut(s) 632, 772, 1321, 1503
Sau3AI GATC 5 cut(s) 496, 527, 1226, 1230, 1489
Sau96I GGNCC 4 cut(s) 219, 247, 467, 492
SchI GAGTC 2 cut(s) 1808, 2233
ScrFI CCNGG 3 cut(s) 813, 998, 2008
SduI GDGCHC 3 cut(s) 297, 1172, 1941
SfaNI GCATC 4 cut(s) 925, 1262, 1293, 2229
SfcI CTRYAG 4 cut(s) 629, 673, 769, 1972
Sfr274I CTCGAG 1 cut(s) 1894
SfuI TTCGAA 1 cut(s) 1958
SinI GGWCC 1 cut(s) 219
SlaI CTCGAG 1 cut(s) 1894
SmiMI CAYNNNNRTG 2 cut(s) 174, 1785
SmlI CTYRAG 2 cut(s) 1359, 1894
SmoI CTYRAG 2 cut(s) 1359, 1894
SseBI AGGCCT 1 cut(s) 1134
SsiI CCGC 6 cut(s) 24, 356, 753, 1041, 1087, 1323
SspI AATATT 1 cut(s) 639
SspMI CTAG 4 cut(s) 278, 783, 1271, 1994
StuI AGGCCT 1 cut(s) 1134
StyD4I CCNGG 3 cut(s) 811, 996, 2006
StyI CCWWGG 4 cut(s) 500, 742, 1665, 1830
TaaI ACNGT 7 cut(s) 109, 214, 601, 620, 677, 1013, 1976
TaiI ACGT 1 cut(s) 928
TaqII GACCGA 1 cut(s) 219
TatI WGTACW 4 cut(s) 785, 1013, 1470, 1647
TfiI GAWTC 4 cut(s) 1020, 1157, 1337, 1431
Tru1I TTAA 4 cut(s) 1245, 1509, 1862, 2172
Tru9I TTAA 4 cut(s) 1245, 1509, 1862, 2172
TscAI CASTG 4 cut(s) 1058, 1228, 1387, 2192
TseFI GTSAC 1 cut(s) 128
TseI GCWGC 4 cut(s) 631, 771, 1320, 1502
Tsp45I GTSAC 1 cut(s) 128
TspDTI ATGAA 7 cut(s) 834, 1221, 1601, 1742, 1770, 2114, 2160
TspGWI ACGGA 1 cut(s) 347
TspRI CASTG 4 cut(s) 1058, 1228, 1387, 2192
VneI GTGCAC 1 cut(s) 1937
VpaK11BI GGWCC 1 cut(s) 219
XagI CCTNNNNNAGG 1 cut(s) 786
XapI RAATTY 4 cut(s) 574, 1031, 1247, 2163
XcmI CCANNNNNNNNNTGG 3 cut(s) 1031, 1034, 1918
XhoI CTCGAG 1 cut(s) 1894
XmiI GTMKAC 2 cut(s) 454, 1971
XmnI GAANNNNTTC 1 cut(s) 1773
XspI CTAG 4 cut(s) 278, 783, 1271, 1994
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.