Rw6G041720

Receptor protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0004846
Physical Location & Seq
Forward (+)
65351310 .. 65354196
2887 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G041720.1

Sequence Viewer

Length: 2394 bp
ATGGACAGGGTTCCGTTTCTGTGCCTTGTTCTTGTTTTTGCACTAATTTTTCCATTCAAATCATCAACAGCTTTTAATTTGAGCGTGGGATCCTTTCTTTCAGTAGAAAATCCATCTCAAACTTTGATGTCTCCGGACGCAGTATTTACCGCTGGCTTTTTTCCGGTCGGTGAAAATGCGTTTTGCTTTGGAATATGGTTCTCGGAGCCATCAACCAGTACTCACAACCTCACCGTGGTGTGGATAGCAAATCGTGACGAGCCGGTCAACGGTCAACGCTCTAAGCTCACCCTGCACAAAAGTGGAACCCTTATTCTCTCCGACATCGACCGATACACCGTGTGGTCCTCCAACACAGCTTCAAACTCATCGGCCCACTTGCACCTTAGAAACACCGGCAATCTTGTTCTGCTCGCTGCGCACGGCGGCGGCGTATTGTGGGAAAGCTTTGCTTCGCCGACGGACACACTTTTGCCTCAGCAAGCGCTCACTAGAAACACACAGCTCATTTCTTCAAGAAGCCTCACCAACTATTCCTCCGGCTTCTACTCTTTTTACTTCGACAACGACAACACTCTTCGTATGCTCTACAACGGCCCCGAGGTTTCGAGCATTTACTGGCCTGATCCATGGCTTCTGAGTTGGCAAGTAGGAAGATCCACATACAACAACAGTCGAATTGCAACTTTTGACAGCTTGGGAAATTTTAGTGCATCTGATAATTTTATCGTTTGGACAATTGACTACGGTGCAAATTTGCAGCGAATATTCAGGATGGATGTAGATGGTAATCTCAGAGGTTACAGTCGAAAAGGTCCTGGAGATAAATGGATTGTTACATGGGAGGCAGTTTTACAACCTTGTAAAATTCATGGCATATGCGGAGCTAATAGCTATTGCAGCTATATTCCTAGTACAGGTAGGAAATGCTCGTGCCTGCCGGGTTATGAAATGATCAGTAAAACCGATTGGTCTTATGGGTGTAAACCGGCTGGTTTCAATCTGTCCTGCAAGAGTAGTGCACGGGAGGAGTTTGGTTTTGTGCATCTTCCACGTATTGAGATGTATGGTTATGATTATGCCGTCTATGGCAATTACACATTAGATGAGTGCAAAAGTTTGTGCCTGGACTTGTGCAACTGTACTGCATTCCACCACAAATTTGGTGGTGGTCAGTATCAGTGTTATCCCAAGGCGCAATTACGAAATGGGTACCGGTCACCTGCTTTTGATGGGGAGCTGTATGTCAAGCTACCAAAAGGCGTCCTCAATGCTACCTATAGAGATACCAACATGTGCTCAGCTAAACTTACAGCACAGCTTGACAGAACATATGAAAGAAGCAAGCCAAATGGATCGATTGAGTTTATGCTGAAATTTGCATCTGCCTTGGGAGGAATAGAGGTTATTTGCATCTTTGTTGTGTGGTTTTTTGTGACAAGAAGAAGCGGTGGAGATAAAAAAGATTCAGAACTAGAACTACTTGCTCAAGGCTACCTTCAAGCTGCCAGTGGATTCAGAAGGTTTACTTACTCCGAGTTGAAGAAGGCCACAAAAGGTTTTAAAGAAGAGATTGGCAGAGGAGGAGGAGGAGTTGTGTACAAAGGCATATTGTCTGATCAACGAGTTGCAGCAATCAAGCAACTGAATGATTGTAAACAAGGGGGTGAAGCAGAGTTCCTTGCAGAAGTGAGCCTTATTGGTAAGCTCTACCATATGAACTTGATAGAGATGTGGGGTTATTGTGTAGAAGACAAGCATAGGCTTCTAGTGTATGAGTACATGGAGCATGGTTCTTTGGCGAAAAATTTATCTTCCAATGTGCTTGATTGGAAGAAGAGGTATGAAATTGCTCTAGGCACAGCGAAAGGCCTGGCTTATCTACATGAAGAGTGCTTGGAATGGGTTTTACACTGTGATGTGAAGCCGCAAAACATACTATTGGACTCCGATTACAATCCAAAGGTAGCTGATTTTGGATTGTCCAAGCTATTAAACAGAGGTGAGAACACCAGCTCGGACTTCTCTAGGATAAGAGGAACCAGAGGTTACATGGCTCCGGAGTGGGTGCACAATATGCCAATCACTTCCAAAGTGGATGTTTACAGTTACGGGATTGTTGTATTGGAGATGCTGACCGGAAAGAACCCAACTGAAAGGAACGTTCAATCTGTTGAAGGTGGAGGGGAGACACAGAAGCAAATAATGGTCAAATGGGTCATGGAGAAAATGGATGGAGCGTTTACAAATGCAGAGACTGAGATGGGAGGTGAAAATGGAGAGGGTAAGCTGGAAATTCTTGTTAAAGTGGCTCTTCAGTGTATAGAGGAAGACAGAAATGCAAGACCCTCAATGAGTCGAGTAGTTGAGATGCTTCTACATCATGAATTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0016020
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin PAN_1 PAN_2 Pkinase S_locus_glycop

Protein Analysis

797

Amino Acids

89.38

Weight (kDa)

6.64

Isoelectric Point (pI)

36.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 78 - 176 5.5e-24 D-mannose binding lectin
S_locus_glycop PF00954 244 - 317 4.5e-07 S-locus glycoprotein domain
PK_Tyr_Ser-Thr PF07714 521 - 792 1.7e-42 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 522 - 790 2.8e-53 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1231
AasI GACNNNNNNGTC 1 cut(s) 263
Acc16I TGCGCA 1 cut(s) 420
Acc36I ACCTGC 1 cut(s) 1231
Acc65I GGTACC 1 cut(s) 1212
AccB1I GGYRCC 1 cut(s) 1212
AccIII TCCGGA 2 cut(s) 133, 2059
AciI CCGC 6 cut(s) 150, 426, 429, 882, 1449, 1928
AclI AACGTT 1 cut(s) 2163
AclWI GGATC 5 cut(s) 84, 97, 620, 651, 1363
AcsI RAATTY 7 cut(s) 703, 754, 867, 1160, 1376, 1807, 2295
AcuI CTGAAG 1 cut(s) 2300
AcyI GRCGYC 1 cut(s) 1263
AdeI CACNNNGTG 1 cut(s) 342
AfaI GTAC 6 cut(s) 220, 916, 1144, 1214, 1601, 1781
AfeI AGCGCT 1 cut(s) 486
AfiI CCNNNNNNNGG 4 cut(s) 235, 240, 269, 917
AflIII ACRYGT 1 cut(s) 1293
AgeI ACCGGT 1 cut(s) 1215
AgsI TTSAA 8 cut(s) 58, 363, 516, 1000, 1502, 1543, 2168, 2177
AjnI CCWGG 3 cut(s) 817, 1125, 1872
AleI CACNNNNGTG 2 cut(s) 236, 300
Alw21I GWGCWC 3 cut(s) 1024, 1301, 2073
Alw26I GTCTC 3 cut(s) 135, 2183, 2249
Alw44I GTGCAC 2 cut(s) 1020, 2069
AlwI GGATC 5 cut(s) 84, 97, 620, 651, 1363
AlwNI CAGNNNCTG 1 cut(s) 2258
Ama87I CYCGRG 1 cut(s) 599
Aor13HI TCCGGA 2 cut(s) 133, 2059
Aor51HI AGCGCT 1 cut(s) 486
AoxI GGCC 5 cut(s) 372, 595, 620, 1548, 1870
ApaLI GTGCAC 2 cut(s) 1020, 2069
ApeKI GCWGC 5 cut(s) 416, 760, 900, 1505, 1631
ApoI RAATTY 7 cut(s) 703, 754, 867, 1160, 1376, 1807, 2295
AsiGI ACCGGT 1 cut(s) 1215
Asp718I GGTACC 1 cut(s) 1212
AspLEI GCGC 3 cut(s) 421, 487, 1198
AspS9I GGNCC 4 cut(s) 345, 373, 596, 815
AsuC2I CCSGG 1 cut(s) 942
AsuHPI GGTGA 8 cut(s) 182, 223, 280, 517, 1212, 1679, 2015, 2282
AvaI CYCGRG 1 cut(s) 599
AvaII GGWCC 2 cut(s) 345, 815
BaeGI GKGCMC 2 cut(s) 1024, 2073
BamHI GGATCC 1 cut(s) 89
BanI GGYRCC 1 cut(s) 1212
BauI CACGAG 1 cut(s) 931
BbsI GAAGAC 2 cut(s) 1758, 2337
Bbv12I GWGCWC 3 cut(s) 1024, 1301, 2073
BbvCI CCTCAGC 1 cut(s) 477
BbvI GCAGC 5 cut(s) 403, 772, 912, 1492, 1643
BccI CCATC 7 cut(s) 121, 217, 769, 779, 1226, 2228, 2257
BceAI ACGGC 3 cut(s) 439, 610, 1067
BciT130I CCWGG 3 cut(s) 819, 1127, 1874
BclI TGATCA 2 cut(s) 954, 1618
BcnI CCSGG 1 cut(s) 942
BcoDI GTCTC 3 cut(s) 135, 2183, 2249
BfaI CTAG 6 cut(s) 492, 912, 1475, 1769, 1856, 2028
BfmI CTRYAG 1 cut(s) 1279
BfoI RGCGCY 1 cut(s) 488
BfuAI ACCTGC 1 cut(s) 1231
BisI GCNGC 8 cut(s) 417, 427, 430, 761, 901, 1506, 1632, 1928
BlpI GCTNAGC 1 cut(s) 1300
BlsI GCNGC 8 cut(s) 418, 428, 431, 762, 902, 1507, 1633, 1929
BmcAI AGTACT 1 cut(s) 220
Bme1390I CCNGG 4 cut(s) 819, 942, 1127, 1874
Bme18I GGWCC 2 cut(s) 345, 815
BmeT110I CYCGRG 1 cut(s) 599
BmgT120I GGNCC 4 cut(s) 345, 373, 596, 815
BmiI GGNNCC 8 cut(s) 12, 91, 207, 307, 598, 1214, 2041, 2058
BmrFI CCNGG 4 cut(s) 819, 942, 1127, 1874
BmsI GCATC 6 cut(s) 722, 1054, 1391, 1422, 2121, 2361
BpiI GAAGAC 2 cut(s) 1758, 2337
BpmI CTGGAG 1 cut(s) 840
Bpu10I CCTNAGC 1 cut(s) 477
Bpu1102I GCTNAGC 1 cut(s) 1300
BpuEI CTTGAG 1 cut(s) 1473
BpuMI CCSGG 1 cut(s) 942
Bsa29I ATCGAT 1 cut(s) 1358
BsaAI YACGTR 1 cut(s) 1055
BsaBI GATNNNNATC 2 cut(s) 789, 1956
BsaHI GRCGYC 1 cut(s) 1263
BsaJI CCNNGG 5 cut(s) 234, 600, 629, 1191, 1389
BsaWI WCCGGW 5 cut(s) 133, 163, 1215, 2059, 2138
BsaXI ACNNNNNCTCC 6 cut(s) 521, 551, 1578, 1581, 1608, 1611
Bsc4I CCNNNNNNNGG 4 cut(s) 235, 240, 269, 917
Bse118I RCCGGY 4 cut(s) 262, 395, 988, 1215
Bse1I ACTGG 3 cut(s) 216, 623, 1509
Bse8I GATNNNNATC 2 cut(s) 789, 1956
BseAI TCCGGA 2 cut(s) 133, 2059
BseBI CCWGG 3 cut(s) 819, 1127, 1874
BseCI ATCGAT 1 cut(s) 1358
BseDI CCNNGG 5 cut(s) 234, 600, 629, 1191, 1389
BseGI GGATG 4 cut(s) 780, 784, 2104, 2239
BseJI GATNNNNATC 2 cut(s) 789, 1956
BseLI CCNNNNNNNGG 4 cut(s) 235, 240, 269, 917
BseMII CTCAG 5 cut(s) 491, 629, 808, 1314, 2250
BseNI ACTGG 3 cut(s) 216, 623, 1509
BseRI GAGGAG 5 cut(s) 1043, 1596, 1599, 1602, 1605
BseSI GKGCMC 2 cut(s) 1024, 2073
BseXI GCAGC 5 cut(s) 403, 772, 912, 1492, 1643
BsgI GTGCAG 1 cut(s) 278
Bsh1285I CGRYCG 2 cut(s) 168, 331
BshFI GGCC 5 cut(s) 374, 597, 622, 1550, 1872
BshNI GGYRCC 1 cut(s) 1212
BshTI ACCGGT 1 cut(s) 1215
BshVI ATCGAT 1 cut(s) 1358
BsiEI CGRYCG 2 cut(s) 168, 331
BsiHKAI GWGCWC 3 cut(s) 1024, 1301, 2073
BsiHKCI CYCGRG 1 cut(s) 599
BslI CCNNNNNNNGG 4 cut(s) 235, 240, 269, 917
BsmAI GTCTC 3 cut(s) 135, 2183, 2249
BsmI GAATGC 1 cut(s) 1148
BsnI GGCC 5 cut(s) 374, 597, 622, 1550, 1872
BsoBI CYCGRG 1 cut(s) 599
Bsp1286I GDGCHC 3 cut(s) 1024, 1301, 2073
Bsp13I TCCGGA 2 cut(s) 133, 2059
Bsp1407I TGTACA 1 cut(s) 1599
Bsp143I GATC 6 cut(s) 89, 625, 656, 954, 1355, 1618
Bsp1720I GCTNAGC 1 cut(s) 1300
Bsp19I CCATGG 1 cut(s) 629
BspACI CCGC 6 cut(s) 150, 426, 429, 882, 1449, 1928
BspANI GGCC 5 cut(s) 374, 597, 622, 1550, 1872
BspCNI CTCAG 5 cut(s) 490, 630, 807, 1313, 2251
BspDI ATCGAT 1 cut(s) 1358
BspEI TCCGGA 2 cut(s) 133, 2059
BspHI TCATGA 1 cut(s) 2383
BspLI GGNNCC 8 cut(s) 12, 91, 207, 307, 598, 1214, 2041, 2058
BspMI ACCTGC 1 cut(s) 1231
BspPI GGATC 5 cut(s) 84, 97, 620, 651, 1363
BspQI GCTCTTC 1 cut(s) 2319
BspT107I GGYRCC 1 cut(s) 1212
BsrFI RCCGGY 4 cut(s) 262, 395, 988, 1215
BsrGI TGTACA 1 cut(s) 1599
BsrI ACTGG 3 cut(s) 216, 623, 1509
BssAI RCCGGY 4 cut(s) 262, 395, 988, 1215
BssECI CCNNGG 5 cut(s) 234, 600, 629, 1191, 1389
BssMI GATC 6 cut(s) 89, 625, 656, 954, 1355, 1618
BssNI GRCGYC 1 cut(s) 1263
BssSI CACGAG 1 cut(s) 931
BssT1I CCWWGG 3 cut(s) 629, 1191, 1389
Bst2BI CACGAG 1 cut(s) 931
Bst2UI CCWGG 3 cut(s) 819, 1127, 1874
Bst4CI ACNGT 9 cut(s) 235, 272, 340, 674, 749, 806, 1142, 1916, 2108
Bst6I CTCTTC 5 cut(s) 582, 1563, 1832, 1884, 2319
BstACI GRCGYC 1 cut(s) 1263
BstAPI GCANNNNNTGC 1 cut(s) 2077
BstAUI TGTACA 1 cut(s) 1599
BstBAI YACGTR 1 cut(s) 1055
BstC8I GCNNGC 5 cut(s) 154, 414, 483, 938, 1346
BstDEI CTNAG 7 cut(s) 282, 386, 477, 638, 794, 1300, 2259
BstDSI CCRYGG 2 cut(s) 234, 629
BstEII GGTNACC 1 cut(s) 1218
BstENI CCTNNNNNAGG 1 cut(s) 915
BstF5I GGATG 4 cut(s) 780, 784, 2104, 2239
BstH2I RGCGCY 1 cut(s) 488
BstHHI GCGC 3 cut(s) 421, 487, 1198
BstKTI GATC 6 cut(s) 92, 628, 659, 957, 1358, 1621
BstMAI GTCTC 3 cut(s) 135, 2183, 2249
BstMBI GATC 6 cut(s) 89, 625, 656, 954, 1355, 1618
BstMCI CGRYCG 2 cut(s) 168, 331
BstMWI GCNNNNNNNGC 4 cut(s) 292, 418, 900, 2077
BstNI CCWGG 3 cut(s) 819, 1127, 1874
BstNSI RCATGY 1 cut(s) 1297
BstPI GGTNACC 1 cut(s) 1218
BstSCI CCNGG 4 cut(s) 817, 940, 1125, 1872
BstSFI CTRYAG 1 cut(s) 1279
BstSLI GKGCMC 2 cut(s) 1024, 2073
BstV1I GCAGC 5 cut(s) 403, 772, 912, 1492, 1643
BstV2I GAAGAC 2 cut(s) 1758, 2337
BstX2I RGATCY 2 cut(s) 89, 656
BstXI CCANNNNNNTGG 1 cut(s) 1163
BstYI RGATCY 2 cut(s) 89, 656
Bsu15I ATCGAT 1 cut(s) 1358
BsuRI GGCC 5 cut(s) 374, 597, 622, 1550, 1872
BsuTUI ATCGAT 1 cut(s) 1358
BtgI CCRYGG 2 cut(s) 234, 629
BtsCI GGATG 4 cut(s) 780, 784, 2104, 2239
BtsIMutI CAGTG 4 cut(s) 1187, 1516, 1912, 2324
BveI ACCTGC 1 cut(s) 1231
Cac8I GCNNGC 5 cut(s) 154, 414, 483, 938, 1346
CaiI CAGNNNCTG 1 cut(s) 2258
CciI TCATGA 1 cut(s) 2383
CfoI GCGC 3 cut(s) 421, 487, 1198
Cfr10I RCCGGY 4 cut(s) 262, 395, 988, 1215
Cfr13I GGNCC 4 cut(s) 345, 373, 596, 815
ClaI ATCGAT 1 cut(s) 1358
CseI GACGC 2 cut(s) 146, 1252
Csp6I GTAC 6 cut(s) 219, 915, 1143, 1213, 1600, 1780
CspAI ACCGGT 1 cut(s) 1215
CspCI CAANNNNNGTGG 2 cut(s) 1147, 1182
CviQI GTAC 6 cut(s) 219, 915, 1143, 1213, 1600, 1780
DdeI CTNAG 7 cut(s) 282, 386, 477, 638, 794, 1300, 2259
DpnI GATC 6 cut(s) 91, 627, 658, 956, 1357, 1620
DpnII GATC 6 cut(s) 89, 625, 656, 954, 1355, 1618
DraI TTTAAA 1 cut(s) 1564
DraIII CACNNNGTG 1 cut(s) 342
DrdI GACNNNNNNGTC 1 cut(s) 263
DseDI GACNNNNNNGTC 1 cut(s) 263
Eam1104I CTCTTC 5 cut(s) 582, 1563, 1832, 1884, 2319
EarI CTCTTC 5 cut(s) 582, 1563, 1832, 1884, 2319
Eco130I CCWWGG 3 cut(s) 629, 1191, 1389
Eco147I AGGCCT 1 cut(s) 1872
Eco47I GGWCC 2 cut(s) 345, 815
Eco47III AGCGCT 1 cut(s) 486
Eco57I CTGAAG 1 cut(s) 2300
Eco88I CYCGRG 1 cut(s) 599
Eco91I GGTNACC 1 cut(s) 1218
EcoNI CCTNNNNNAGG 1 cut(s) 915
EcoO109I RGGNCCY 1 cut(s) 815
EcoO65I GGTNACC 1 cut(s) 1218
EcoRII CCWGG 3 cut(s) 817, 1125, 1872
EcoT14I CCWWGG 3 cut(s) 629, 1191, 1389
ErhI CCWWGG 3 cut(s) 629, 1191, 1389
FauNDI CATATG 3 cut(s) 878, 1333, 1716
FbaI TGATCA 2 cut(s) 954, 1618
Fnu4HI GCNGC 8 cut(s) 417, 427, 430, 761, 901, 1506, 1632, 1928
FokI GGATG 4 cut(s) 787, 791, 2111, 2246
Fsp4HI GCNGC 8 cut(s) 417, 427, 430, 761, 901, 1506, 1632, 1928
FspBI CTAG 6 cut(s) 492, 912, 1475, 1769, 1856, 2028
FspI TGCGCA 1 cut(s) 420
GlaI GCGC 3 cut(s) 420, 486, 1197
GluI GCNGC 8 cut(s) 417, 427, 430, 761, 901, 1506, 1632, 1928
GsuI CTGGAG 1 cut(s) 840
HaeII RGCGCY 1 cut(s) 488
HaeIII GGCC 5 cut(s) 374, 597, 622, 1550, 1872
HgaI GACGC 2 cut(s) 146, 1252
HhaI GCGC 3 cut(s) 421, 487, 1198
Hin1I GRCGYC 1 cut(s) 1263
Hin6I GCGC 3 cut(s) 419, 485, 1196
HinP1I GCGC 3 cut(s) 419, 485, 1196
HincII GTYRAC 2 cut(s) 268, 275
HindII GTYRAC 2 cut(s) 268, 275
HindIII AAGCTT 1 cut(s) 445
HinfI GANTC 4 cut(s) 1466, 1515, 1946, 2356
HphI GGTGA 8 cut(s) 182, 223, 280, 517, 1212, 1679, 2015, 2282
Hpy188III TCNNGA 6 cut(s) 134, 254, 516, 772, 2060, 2384
Hpy99I CGWCG 1 cut(s) 463
HpyAV CCTTC 4 cut(s) 1508, 1515, 1540, 2171
HpyCH4III ACNGT 9 cut(s) 235, 272, 340, 674, 749, 806, 1142, 1916, 2108
HpyCH4IV ACGT 2 cut(s) 1054, 2163
HpyF10VI GCNNNNNNNGC 4 cut(s) 292, 418, 900, 2077
HpyF3I CTNAG 7 cut(s) 282, 386, 477, 638, 794, 1300, 2259
HpySE526I ACGT 2 cut(s) 1054, 2163
Hsp92I GRCGYC 1 cut(s) 1263
HspAI GCGC 3 cut(s) 419, 485, 1196
Kpn2I TCCGGA 2 cut(s) 133, 2059
KpnI GGTACC 1 cut(s) 1216
Ksp22I TGATCA 2 cut(s) 954, 1618
Kzo9I GATC 6 cut(s) 89, 625, 656, 954, 1355, 1618
LguI GCTCTTC 1 cut(s) 2319
LmnI GCTCC 6 cut(s) 205, 884, 1237, 1786, 2062, 2237
Lsp1109I GCAGC 5 cut(s) 403, 772, 912, 1492, 1643
LweI GCATC 6 cut(s) 722, 1054, 1391, 1422, 2121, 2361
MaeI CTAG 6 cut(s) 492, 912, 1475, 1769, 1856, 2028
MaeII ACGT 2 cut(s) 1054, 2163
MaeIII GTNAC 7 cut(s) 254, 800, 835, 1218, 1435, 2048, 2108
MalI GATC 6 cut(s) 91, 627, 658, 956, 1357, 1620
MboI GATC 6 cut(s) 89, 625, 656, 954, 1355, 1618
MfeI CAATTG 1 cut(s) 738
MflI RGATCY 2 cut(s) 89, 656
MhlI GDGCHC 3 cut(s) 1024, 1301, 2073
MlyI GAGTC 2 cut(s) 1940, 2365
MmeI TCCRAC 2 cut(s) 345, 375
MroI TCCGGA 2 cut(s) 133, 2059
MseI TTAA 4 cut(s) 75, 1563, 1994, 2304
MslI CAYNNNNRTG 3 cut(s) 236, 300, 1917
MspA1I CMGCKG 1 cut(s) 152
MspR9I CCNGG 4 cut(s) 819, 942, 1127, 1874
MunI CAATTG 1 cut(s) 738
Mva1269I GAATGC 1 cut(s) 1148
MvaI CCWGG 3 cut(s) 819, 1127, 1874
MwoI GCNNNNNNNGC 4 cut(s) 292, 418, 900, 2077
NciI CCSGG 1 cut(s) 942
NcoI CCATGG 1 cut(s) 629
NdeI CATATG 3 cut(s) 878, 1333, 1716
NdeII GATC 6 cut(s) 89, 625, 656, 954, 1355, 1618
NlaIV GGNNCC 8 cut(s) 12, 91, 207, 307, 598, 1214, 2041, 2058
NmuCI GTSAC 3 cut(s) 254, 1218, 1435
NsbI TGCGCA 1 cut(s) 420
NspI RCATGY 1 cut(s) 1297
OliI CACNNNNGTG 2 cut(s) 236, 300
PagI TCATGA 1 cut(s) 2383
PaqCI CACCTGC 1 cut(s) 1231
PceI AGGCCT 1 cut(s) 1872
PciI ACATGT 1 cut(s) 1293
PciSI GCTCTTC 1 cut(s) 2319
PcsI WCGNNNNNNNCGW 1 cut(s) 429
PctI GAATGC 1 cut(s) 1148
PfeI GAWTC 2 cut(s) 1466, 1515
PfoI TCCNGGA 1 cut(s) 817
PinAI ACCGGT 1 cut(s) 1215
PkrI GCNGC 8 cut(s) 418, 428, 431, 762, 902, 1507, 1633, 1929
PleI GAGTC 2 cut(s) 1940, 2364
PpsI GAGTC 2 cut(s) 1940, 2364
Ppu21I YACGTR 1 cut(s) 1055
PpuMI RGGWCCY 1 cut(s) 815
PscI ACATGT 1 cut(s) 1293
Psp1406I AACGTT 1 cut(s) 2163
Psp5II RGGWCCY 1 cut(s) 815
Psp6I CCWGG 3 cut(s) 817, 1125, 1872
PspEI GGTNACC 1 cut(s) 1218
PspGI CCWGG 3 cut(s) 817, 1125, 1872
PspN4I GGNNCC 8 cut(s) 12, 91, 207, 307, 598, 1214, 2041, 2058
PspPI GGNCC 4 cut(s) 345, 373, 596, 815
PspPPI RGGWCCY 1 cut(s) 815
PsrI GAACNNNNNNTAC 2 cut(s) 1464, 1496
PstNI CAGNNNCTG 1 cut(s) 2258
PsuI RGATCY 2 cut(s) 89, 656
RsaI GTAC 6 cut(s) 220, 916, 1144, 1214, 1601, 1781
RsaNI GTAC 6 cut(s) 219, 915, 1143, 1213, 1600, 1780
RseI CAYNNNNRTG 3 cut(s) 236, 300, 1917
SapI GCTCTTC 1 cut(s) 2319
SaqAI TTAA 4 cut(s) 75, 1563, 1994, 2304
SatI GCNGC 8 cut(s) 417, 427, 430, 761, 901, 1506, 1632, 1928
Sau3AI GATC 6 cut(s) 89, 625, 656, 954, 1355, 1618
Sau96I GGNCC 4 cut(s) 345, 373, 596, 815
ScaI AGTACT 1 cut(s) 220
SchI GAGTC 2 cut(s) 1940, 2365
ScrFI CCNGG 4 cut(s) 819, 942, 1127, 1874
SduI GDGCHC 3 cut(s) 1024, 1301, 2073
SfaNI GCATC 6 cut(s) 722, 1054, 1391, 1422, 2121, 2361
SfcI CTRYAG 1 cut(s) 1279
SinI GGWCC 2 cut(s) 345, 815
SmiMI CAYNNNNRTG 3 cut(s) 236, 300, 1917
SmlI CTYRAG 1 cut(s) 1488
SmoI CTYRAG 1 cut(s) 1488
SseBI AGGCCT 1 cut(s) 1872
SsiI CCGC 6 cut(s) 150, 426, 429, 882, 1449, 1928
SspI AATATT 1 cut(s) 768
SspMI CTAG 6 cut(s) 492, 912, 1475, 1769, 1856, 2028
StuI AGGCCT 1 cut(s) 1872
StyD4I CCNGG 4 cut(s) 817, 940, 1125, 1872
StyI CCWWGG 3 cut(s) 629, 1191, 1389
TaaI ACNGT 9 cut(s) 235, 272, 340, 674, 749, 806, 1142, 1916, 2108
TaiI ACGT 2 cut(s) 1057, 2166
TaqI TCGA 7 cut(s) 327, 561, 608, 676, 808, 1358, 2359
TaqII GACCGA 1 cut(s) 345
TatI WGTACW 5 cut(s) 218, 914, 1142, 1599, 1779
TauI GCSGC 3 cut(s) 429, 432, 1930
TfiI GAWTC 2 cut(s) 1466, 1515
Tru1I TTAA 4 cut(s) 75, 1563, 1994, 2304
Tru9I TTAA 4 cut(s) 75, 1563, 1994, 2304
TscAI CASTG 4 cut(s) 1187, 1516, 1919, 2324
TseFI GTSAC 3 cut(s) 254, 1218, 1435
TseI GCWGC 5 cut(s) 416, 760, 900, 1505, 1631
Tsp45I GTSAC 3 cut(s) 254, 1218, 1435
TspDTI ATGAA 6 cut(s) 860, 963, 1350, 1733, 1860, 1902
TspGWI ACGGA 1 cut(s) 476
TspRI CASTG 4 cut(s) 1187, 1516, 1919, 2324
VneI GTGCAC 2 cut(s) 1020, 2069
VpaK11BI GGWCC 2 cut(s) 345, 815
XagI CCTNNNNNAGG 1 cut(s) 915
XapI RAATTY 7 cut(s) 703, 754, 867, 1160, 1376, 1807, 2295
XceI RCATGY 1 cut(s) 1297
XcmI CCANNNNNNNNNTGG 3 cut(s) 1160, 1163, 2050
XspI CTAG 6 cut(s) 492, 912, 1475, 1769, 1856, 2028
ZrmI AGTACT 1 cut(s) 220
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.