Rh6CG493600

receptor protein kinase ZmPK1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0004846
Physical Location & Seq
Forward (+)
65954408 .. 65962264
7857 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG493600.1

Sequence Viewer

Length: 2313 bp
ATGGGATCCTTTCTTTCAGTAGAAGATCCATCTCAAACTTTGATGTCTCCGGACGCAGTATTTACAGCTGGCTTTTTTCCGGTCGGTGAAAATGCGTTTTGCTTCGGAATATGGTTCTCGGAGCCATCAACCAGTACTCTCAACCTCACCGTGGTGTGGATAGCAAATCGTGACGAGCCGGTCAACGGTCAACGCTCCAAGCTCACCCTGCACAGAAGTGGAAACCTTATTCTATCCGACATCAACAGATACACCGTGTGGTCCTCCAATACAGCTTCAAACTCGTCGGCCCACTTGCACCTCAGAAACACAGGCAATCTTGTACTGCTCGCTGCGCATGGCGTCGTATTGTGGGAAAGCTTTGCTTCGCCAACGGACACACTTTTACCTCAGCAAGCGCTCACTAGAAACACACAGCTCATTTCTTCAAGAAGCCTCACCAACTATTCCTCCGGCTTCTACTCTCTTTACTTCGACGACGACAACACTCTTCGTATGTTCTACAACGCCCCCAAGGTTTCGAGCATTTACTGGCCCGATCCATGGCTTCTGAGTCGGCAAGTAGGAAGATCCGCATTCAACAATAGTCGAATTGCAACTTTTGACAACTTGGGAAGTTTTACTGCATCTGATAATTTTATCGTTTGGACAATCGACTACGGCACAAAATTGCAGCGAATATTCAGGATAGATGTAGATGGTAATCTCCGAGGTTACAGTCGAAAAGGTCCTGGAGATAAATGGATTGTTACATGGGAAGCATTTCTACAACCATGTAAAATTCATGGCATTTGCGGAGCTAATAGCTATTGCAGCTATATTCCTAATACAGGTAGGAAATGCTCATGCCTACCGGGTTATGAAATGATCAGTAAAACCGACTGGTCTTATGGGTGTAAACCGGCTGGTTTCAATCTGTCCTGCAAGAGTAGTGCAGAGGAGGAGTTTGGTTTTGTGCATCTTTCACGTATTGAGATGTATGGGTATGATTATGACGTCACTGGCAATTACACGTTTGATGAGTGCAAAAGTTTGTGCCTGGACTTGTGCGACTGTACTGGATTCCTGCACATATTTGGGGGTCGTCGGTATCTGTGTTATCCCAAGGTGCAATTTCGAAATGGGTACCGGTCACCTGCTTTTGGTGGGGAGCTGTATGTGAAGGTACCAAAACGCCTCTTCAATGCTACCTATAGAGATACCAACATGTGCTCAGCTAAACTTCCAGCACAGCTTGACAGAACATATGAAAGAAGCATGCCCAATGGATCAATTGAGTTTATGCTGAAATTTGCATCTGTCTTGGGAGGAATAGAGGTCATTTGCATCTTTGTTGTGTGGTTTTTTGTGACAAGAAGAAGCTGTGGAGATAAAAAAGATTCAGAATTAGAACTACTTGCTCAAGGATACCTTCAAGCTGCCAGTGGATTCAGAAGGTTTACATACTCCGAGTTGAAGAGGGCCACAAAAGGTTTCAAAGAAGAGATTGGCAGAGGAGGAGGAGGAGTTGTGTACAAAGGCATATTGTCTGATCAACGAGTTGCAGCGATCAAGCAACTGAATGATTGCAAACAAGGGGGTGAAGCGGAGTTCCTTGCAGAAGTCAGCCTTATTGGTAAGCTCTACCATATGAACTTGATAGAGATGTGGGGTTATTGCGTGGAAAACAAGCATAGGCTTCTAGTGTATGAGTACATGGAGCATGGTTCTTTGGCGAAAAATTTATCTTCCAGTGTGCTTGATAGGAAGAAGAGTTACGGGATTGTTGTATTGGAGATGCTGACCGGAAAGAACCCAACTGAAAGCAACGTTCAATCTGTTGAAGGTGGAGGGGAGACTCAGAAGCAAATAATGGTCAAATGGGTCATGGAGAAAATGGATGGAGCGTTTACAAATGCAGAGACTGAGATGGGAGGTGAAAATGGAGAGGGCGACAATGCATATTGCTTTGCCATCTGGTTTACCGAGCCAACAACACCAACTCCCAACCTCACCGTGCCACTAACAAGGAACAGCAAGCTCGTATCTTCTAGAATCCTCACCAACTCCTCCTCCGGCTTCTACAAGCTCTTTTTCGATAATGACGACACCCTCCGGCTTCTCTACGATGGCCCCGAAGTTTCGAGTATTTACTGGCCTGACCCTTGGCTTCTAAATTACCAAGCTGAGAGGTCCTCGTACAACAACAGTAGAATCGCAATGTTCGATGCATTAGGGCACCTCAGTGCATCCGACGACTTTACCGTTTTGTCCTCGGATTACGGTGCCAAGGTGCAGAGAATTGTGAAGGTGGATGTGGATGGTAACATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0016020
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin PAN_1 PAN_2 Pkinase S_locus_glycop

Protein Analysis

770

Amino Acids

86.46

Weight (kDa)

6.32

Isoelectric Point (pI)

39.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 50 - 147 2.5e-24 D-mannose binding lectin
S_locus_glycop PF00954 208 - 288 5.9e-07 S-locus glycoprotein domain
PK_Tyr_Ser-Thr PF07714 492 - 578 7e-14 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 493 - 577 1.3e-13 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1144
AasI GACNNNNNNGTC 1 cut(s) 179
AatII GACGTC 1 cut(s) 999
Acc16I TGCGCA 1 cut(s) 336
Acc36I ACCTGC 1 cut(s) 1144
Acc65I GGTACC 2 cut(s) 1125, 1165
AccB1I GGYRCC 4 cut(s) 1125, 1165, 2217, 2264
AccIII TCCGGA 1 cut(s) 49
AciI CCGC 3 cut(s) 573, 795, 1586
AclI AACGTT 1 cut(s) 1809
AclWI GGATC 5 cut(s) 13, 20, 533, 564, 1276
AcsI RAATTY 3 cut(s) 780, 1289, 1720
AcyI GRCGYC 2 cut(s) 342, 996
AdeI CACNNNGTG 1 cut(s) 258
AfaI GTAC 8 cut(s) 136, 324, 1057, 1127, 1167, 1514, 1694, 2180
AfeI AGCGCT 1 cut(s) 399
AfiI CCNNNNNNNGG 6 cut(s) 151, 156, 185, 543, 830, 1142
AflIII ACRYGT 2 cut(s) 1011, 1206
AgeI ACCGGT 1 cut(s) 1128
AjnI CCWGG 2 cut(s) 730, 1038
AleI CACNNNNGTG 3 cut(s) 152, 216, 2223
Alw21I GWGCWC 1 cut(s) 1214
Alw26I GTCTC 3 cut(s) 51, 1829, 1895
AlwI GGATC 5 cut(s) 13, 20, 533, 564, 1276
AlwNI CAGNNNCTG 1 cut(s) 1904
Aor13HI TCCGGA 1 cut(s) 49
Aor51HI AGCGCT 1 cut(s) 399
AoxI GGCC 5 cut(s) 288, 533, 1461, 2110, 2135
ApeKI GCWGC 5 cut(s) 332, 673, 813, 1418, 1544
ApoI RAATTY 3 cut(s) 780, 1289, 1720
AsiGI ACCGGT 1 cut(s) 1128
Asp700I GAANNNNTTC 1 cut(s) 762
Asp718I GGTACC 2 cut(s) 1125, 1165
AspLEI GCGC 2 cut(s) 337, 400
AspS9I GGNCC 7 cut(s) 261, 289, 534, 728, 1461, 2111, 2172
AsuC2I CCSGG 1 cut(s) 855
AsuHPI GGTGA 9 cut(s) 98, 139, 196, 430, 1125, 1592, 1928, 1984, 2032
AsuII TTCGAA 1 cut(s) 1117
AvaII GGWCC 3 cut(s) 261, 728, 2172
BaeGI GKGCMC 1 cut(s) 2220
BamHI GGATCC 1 cut(s) 5
BanI GGYRCC 4 cut(s) 1125, 1165, 2217, 2264
BarI GAAGNNNNNNTAC 4 cut(s) 750, 782, 1739, 1771
Bbv12I GWGCWC 1 cut(s) 1214
BbvCI CCTCAGC 1 cut(s) 390
BbvI GCAGC 5 cut(s) 319, 685, 825, 1405, 1556
BccI CCATC 8 cut(s) 37, 133, 692, 1874, 1903, 1961, 2102, 2294
BceAI ACGGC 1 cut(s) 676
BciT130I CCWGG 2 cut(s) 732, 1040
BciVI GTATCC 1 cut(s) 1400
BclI TGATCA 2 cut(s) 867, 1531
BcnI CCSGG 1 cut(s) 855
BcoDI GTCTC 3 cut(s) 51, 1829, 1895
BfaI CTAG 3 cut(s) 405, 1682, 2031
BfmI CTRYAG 1 cut(s) 1192
BfoI RGCGCY 1 cut(s) 401
BfuAI ACCTGC 1 cut(s) 1144
BfuI GTATCC 1 cut(s) 1400
BisI GCNGC 5 cut(s) 333, 674, 814, 1419, 1545
BlpI GCTNAGC 1 cut(s) 1213
BlsI GCNGC 5 cut(s) 334, 675, 815, 1420, 1546
BmcAI AGTACT 1 cut(s) 136
Bme1390I CCNGG 3 cut(s) 732, 855, 1040
Bme18I GGWCC 3 cut(s) 261, 728, 2172
BmgT120I GGNCC 7 cut(s) 261, 289, 534, 728, 1461, 2111, 2172
BmiI GGNNCC 7 cut(s) 7, 123, 1127, 1167, 2113, 2219, 2266
BmrFI CCNGG 3 cut(s) 732, 855, 1040
BmsI GCATC 7 cut(s) 635, 967, 1304, 1335, 1767, 2197, 2237
BplI GAGNNNNNCTC 2 cut(s) 2159, 2191
BpmI CTGGAG 1 cut(s) 753
Bpu10I CCTNAGC 1 cut(s) 390
Bpu1102I GCTNAGC 1 cut(s) 1213
Bpu14I TTCGAA 1 cut(s) 1117
BpuEI CTTGAG 1 cut(s) 1386
BpuMI CCSGG 1 cut(s) 855
BsaAI YACGTR 1 cut(s) 968
BsaBI GATNNNNATC 1 cut(s) 702
BsaHI GRCGYC 2 cut(s) 342, 996
BsaJI CCNNGG 8 cut(s) 150, 513, 542, 709, 1104, 2144, 2253, 2268
BsaWI WCCGGW 4 cut(s) 49, 79, 1128, 1784
Bsc4I CCNNNNNNNGG 6 cut(s) 151, 156, 185, 543, 830, 1142
Bse118I RCCGGY 3 cut(s) 178, 901, 1128
Bse1I ACTGG 8 cut(s) 132, 536, 887, 1006, 1063, 1422, 1731, 2138
Bse3DI GCAATG 1 cut(s) 2205
Bse8I GATNNNNATC 1 cut(s) 702
BseAI TCCGGA 1 cut(s) 49
BseBI CCWGG 2 cut(s) 732, 1040
BseDI CCNNGG 8 cut(s) 150, 513, 542, 709, 1104, 2144, 2253, 2268
BseGI GGATG 4 cut(s) 1885, 2228, 2299, 2305
BseJI GATNNNNATC 1 cut(s) 702
BseLI CCNNNNNNNGG 6 cut(s) 151, 156, 185, 543, 830, 1142
BseMI GCAATG 1 cut(s) 2205
BseMII CTCAG 8 cut(s) 316, 404, 542, 1227, 1853, 1896, 2157, 2236
BseNI ACTGG 8 cut(s) 132, 536, 887, 1006, 1063, 1422, 1731, 2138
BseRI GAGGAG 8 cut(s) 953, 956, 1509, 1512, 1515, 1518, 2038, 2041
BseSI GKGCMC 1 cut(s) 2220
BseXI GCAGC 5 cut(s) 319, 685, 825, 1405, 1556
BsgI GTGCAG 4 cut(s) 194, 954, 1052, 2294
Bsh1285I CGRYCG 1 cut(s) 84
BshFI GGCC 5 cut(s) 290, 535, 1463, 2112, 2137
BshNI GGYRCC 4 cut(s) 1125, 1165, 2217, 2264
BshTI ACCGGT 1 cut(s) 1128
BsiEI CGRYCG 1 cut(s) 84
BsiHKAI GWGCWC 1 cut(s) 1214
BslI CCNNNNNNNGG 6 cut(s) 151, 156, 185, 543, 830, 1142
BsmAI GTCTC 3 cut(s) 51, 1829, 1895
BsmI GAATGC 1 cut(s) 575
BsnI GGCC 5 cut(s) 290, 535, 1463, 2112, 2137
Bsp119I TTCGAA 1 cut(s) 1117
Bsp1286I GDGCHC 2 cut(s) 1214, 2220
Bsp13I TCCGGA 1 cut(s) 49
Bsp1407I TGTACA 1 cut(s) 1512
Bsp143I GATC 8 cut(s) 5, 25, 538, 569, 867, 1268, 1531, 1548
Bsp1720I GCTNAGC 1 cut(s) 1213
Bsp19I CCATGG 1 cut(s) 542
BspACI CCGC 3 cut(s) 573, 795, 1586
BspANI GGCC 5 cut(s) 290, 535, 1463, 2112, 2137
BspCNI CTCAG 8 cut(s) 315, 403, 543, 1226, 1852, 1897, 2158, 2235
BspEI TCCGGA 1 cut(s) 49
BspLI GGNNCC 7 cut(s) 7, 123, 1127, 1167, 2113, 2219, 2266
BspMI ACCTGC 1 cut(s) 1144
BspPI GGATC 5 cut(s) 13, 20, 533, 564, 1276
BspT104I TTCGAA 1 cut(s) 1117
BspT107I GGYRCC 4 cut(s) 1125, 1165, 2217, 2264
BsrDI GCAATG 1 cut(s) 2205
BsrFI RCCGGY 3 cut(s) 178, 901, 1128
BsrGI TGTACA 1 cut(s) 1512
BsrI ACTGG 8 cut(s) 132, 536, 887, 1006, 1063, 1422, 1731, 2138
BssAI RCCGGY 3 cut(s) 178, 901, 1128
BssECI CCNNGG 8 cut(s) 150, 513, 542, 709, 1104, 2144, 2253, 2268
BssMI GATC 8 cut(s) 5, 25, 538, 569, 867, 1268, 1531, 1548
BssNI GRCGYC 2 cut(s) 342, 996
BssT1I CCWWGG 5 cut(s) 513, 542, 1104, 2144, 2268
Bst2UI CCWGG 2 cut(s) 732, 1040
Bst4CI ACNGT 9 cut(s) 151, 188, 256, 719, 1055, 1996, 2189, 2245, 2264
Bst6I CTCTTC 5 cut(s) 495, 1184, 1451, 1476, 1745
BstACI GRCGYC 2 cut(s) 342, 996
BstAUI TGTACA 1 cut(s) 1512
BstBAI YACGTR 1 cut(s) 968
BstBI TTCGAA 1 cut(s) 1117
BstC8I GCNNGC 5 cut(s) 70, 330, 396, 1259, 2018
BstDEI CTNAG 8 cut(s) 302, 390, 551, 1213, 1839, 1905, 2166, 2222
BstDSI CCRYGG 2 cut(s) 150, 542
BstEII GGTNACC 1 cut(s) 1131
BstENI CCTNNNNNAGG 1 cut(s) 828
BstF5I GGATG 4 cut(s) 1885, 2228, 2299, 2305
BstH2I RGCGCY 1 cut(s) 401
BstHHI GCGC 2 cut(s) 337, 400
BstKTI GATC 8 cut(s) 8, 28, 541, 572, 870, 1271, 1534, 1551
BstMAI GTCTC 3 cut(s) 51, 1829, 1895
BstMBI GATC 8 cut(s) 5, 25, 538, 569, 867, 1268, 1531, 1548
BstMCI CGRYCG 1 cut(s) 84
BstMWI GCNNNNNNNGC 3 cut(s) 208, 334, 813
BstNI CCWGG 2 cut(s) 732, 1040
BstNSI RCATGY 2 cut(s) 1210, 1261
BstPI GGTNACC 1 cut(s) 1131
BstSCI CCNGG 3 cut(s) 730, 853, 1038
BstSFI CTRYAG 1 cut(s) 1192
BstSLI GKGCMC 1 cut(s) 2220
BstV1I GCAGC 5 cut(s) 319, 685, 825, 1405, 1556
BstX2I RGATCY 3 cut(s) 5, 25, 569
BstYI RGATCY 3 cut(s) 5, 25, 569
BsuI GTATCC 1 cut(s) 1400
BsuRI GGCC 5 cut(s) 290, 535, 1463, 2112, 2137
BtgI CCRYGG 2 cut(s) 150, 542
BtsCI GGATG 4 cut(s) 1885, 2228, 2299, 2305
BtsIMutI CAGTG 4 cut(s) 999, 1429, 1738, 2230
BveI ACCTGC 1 cut(s) 1144
Cac8I GCNNGC 5 cut(s) 70, 330, 396, 1259, 2018
CaiI CAGNNNCTG 1 cut(s) 1904
CfoI GCGC 2 cut(s) 337, 400
Cfr10I RCCGGY 3 cut(s) 178, 901, 1128
Cfr13I GGNCC 7 cut(s) 261, 289, 534, 728, 1461, 2111, 2172
CseI GACGC 2 cut(s) 62, 331
Csp6I GTAC 8 cut(s) 135, 323, 1056, 1126, 1166, 1513, 1693, 2179
CspAI ACCGGT 1 cut(s) 1128
CviQI GTAC 8 cut(s) 135, 323, 1056, 1126, 1166, 1513, 1693, 2179
DdeI CTNAG 8 cut(s) 302, 390, 551, 1213, 1839, 1905, 2166, 2222
DpnI GATC 8 cut(s) 7, 27, 540, 571, 869, 1270, 1533, 1550
DpnII GATC 8 cut(s) 5, 25, 538, 569, 867, 1268, 1531, 1548
DraIII CACNNNGTG 1 cut(s) 258
DrdI GACNNNNNNGTC 1 cut(s) 179
DseDI GACNNNNNNGTC 1 cut(s) 179
Eam1104I CTCTTC 5 cut(s) 495, 1184, 1451, 1476, 1745
EarI CTCTTC 5 cut(s) 495, 1184, 1451, 1476, 1745
Eco130I CCWWGG 5 cut(s) 513, 542, 1104, 2144, 2268
Eco47I GGWCC 3 cut(s) 261, 728, 2172
Eco47III AGCGCT 1 cut(s) 399
Eco91I GGTNACC 1 cut(s) 1131
EcoNI CCTNNNNNAGG 1 cut(s) 828
EcoO109I RGGNCCY 2 cut(s) 728, 2172
EcoO65I GGTNACC 1 cut(s) 1131
EcoRII CCWGG 2 cut(s) 730, 1038
EcoT14I CCWWGG 5 cut(s) 513, 542, 1104, 2144, 2268
EcoT22I ATGCAT 2 cut(s) 1942, 2212
ErhI CCWWGG 5 cut(s) 513, 542, 1104, 2144, 2268
FalI AAGNNNNNCTT 6 cut(s) 349, 381, 1395, 1427, 1593, 1625
FauNDI CATATG 2 cut(s) 1246, 1629
FbaI TGATCA 2 cut(s) 867, 1531
Fnu4HI GCNGC 5 cut(s) 333, 674, 814, 1419, 1545
FokI GGATG 3 cut(s) 1892, 2215, 2306
Fsp4HI GCNGC 5 cut(s) 333, 674, 814, 1419, 1545
FspBI CTAG 3 cut(s) 405, 1682, 2031
FspI TGCGCA 1 cut(s) 336
GlaI GCGC 2 cut(s) 336, 399
GluI GCNGC 5 cut(s) 333, 674, 814, 1419, 1545
GsuI CTGGAG 1 cut(s) 753
HaeII RGCGCY 1 cut(s) 401
HaeIII GGCC 5 cut(s) 290, 535, 1463, 2112, 2137
HgaI GACGC 2 cut(s) 62, 331
HhaI GCGC 2 cut(s) 337, 400
Hin1I GRCGYC 2 cut(s) 342, 996
Hin6I GCGC 2 cut(s) 335, 398
HinP1I GCGC 2 cut(s) 335, 398
HincII GTYRAC 2 cut(s) 184, 191
HindII GTYRAC 2 cut(s) 184, 191
HindIII AAGCTT 1 cut(s) 358
HinfI GANTC 7 cut(s) 553, 1062, 1379, 1428, 1837, 2034, 2193
HphI GGTGA 9 cut(s) 98, 139, 196, 430, 1125, 1592, 1928, 1984, 2032
Hpy166II GTNNAC 7 cut(s) 184, 191, 899, 1440, 1513, 1890, 1962
Hpy188III TCNNGA 5 cut(s) 50, 170, 429, 685, 2031
Hpy8I GTNNAC 7 cut(s) 184, 191, 899, 1440, 1513, 1890, 1962
Hpy99I CGWCG 6 cut(s) 289, 347, 479, 482, 1089, 2237
HpyAV CCTTC 5 cut(s) 1156, 1421, 1428, 1817, 2281
HpyCH4III ACNGT 9 cut(s) 151, 188, 256, 719, 1055, 1996, 2189, 2245, 2264
HpyCH4IV ACGT 4 cut(s) 967, 996, 1013, 1809
HpyF10VI GCNNNNNNNGC 3 cut(s) 208, 334, 813
HpyF3I CTNAG 8 cut(s) 302, 390, 551, 1213, 1839, 1905, 2166, 2222
HpySE526I ACGT 4 cut(s) 967, 996, 1013, 1809
Hsp92I GRCGYC 2 cut(s) 342, 996
HspAI GCGC 2 cut(s) 335, 398
Kpn2I TCCGGA 1 cut(s) 49
KpnI GGTACC 2 cut(s) 1129, 1169
Ksp22I TGATCA 2 cut(s) 867, 1531
Kzo9I GATC 8 cut(s) 5, 25, 538, 569, 867, 1268, 1531, 1548
LmnI GCTCC 6 cut(s) 121, 200, 797, 1150, 1699, 1883
Lsp1109I GCAGC 5 cut(s) 319, 685, 825, 1405, 1556
LweI GCATC 7 cut(s) 635, 967, 1304, 1335, 1767, 2197, 2237
MaeI CTAG 3 cut(s) 405, 1682, 2031
MaeII ACGT 4 cut(s) 967, 996, 1013, 1809
MaeIII GTNAC 8 cut(s) 170, 713, 748, 997, 1131, 1348, 1754, 2303
MalI GATC 8 cut(s) 7, 27, 540, 571, 869, 1270, 1533, 1550
MboI GATC 8 cut(s) 5, 25, 538, 569, 867, 1268, 1531, 1548
MfeI CAATTG 1 cut(s) 1272
MflI RGATCY 3 cut(s) 5, 25, 569
MhlI GDGCHC 2 cut(s) 1214, 2220
MlyI GAGTC 2 cut(s) 562, 1831
MmeI TCCRAC 2 cut(s) 261, 2256
Mph1103I ATGCAT 2 cut(s) 1942, 2212
MroI TCCGGA 1 cut(s) 49
MroXI GAANNNNTTC 1 cut(s) 762
MslI CAYNNNNRTG 3 cut(s) 152, 216, 2223
MspA1I CMGCKG 1 cut(s) 68
MspR9I CCNGG 3 cut(s) 732, 855, 1040
MunI CAATTG 1 cut(s) 1272
Mva1269I GAATGC 1 cut(s) 575
MvaI CCWGG 2 cut(s) 732, 1040
MwoI GCNNNNNNNGC 3 cut(s) 208, 334, 813
NciI CCSGG 1 cut(s) 855
NcoI CCATGG 1 cut(s) 542
NdeI CATATG 2 cut(s) 1246, 1629
NdeII GATC 8 cut(s) 5, 25, 538, 569, 867, 1268, 1531, 1548
NlaIV GGNNCC 7 cut(s) 7, 123, 1127, 1167, 2113, 2219, 2266
NmuCI GTSAC 4 cut(s) 170, 997, 1131, 1348
NsbI TGCGCA 1 cut(s) 336
NsiI ATGCAT 2 cut(s) 1942, 2212
NspI RCATGY 2 cut(s) 1210, 1261
NspV TTCGAA 1 cut(s) 1117
OliI CACNNNNGTG 3 cut(s) 152, 216, 2223
PaeI GCATGC 1 cut(s) 1261
PaqCI CACCTGC 1 cut(s) 1144
PciI ACATGT 1 cut(s) 1206
PcsI WCGNNNNNNNCGW 4 cut(s) 2082, 2112, 2202, 2241
PctI GAATGC 1 cut(s) 575
PdmI GAANNNNTTC 1 cut(s) 762
PfeI GAWTC 5 cut(s) 1062, 1379, 1428, 2034, 2193
PfoI TCCNGGA 1 cut(s) 730
PinAI ACCGGT 1 cut(s) 1128
PkrI GCNGC 5 cut(s) 334, 675, 815, 1420, 1546
PleI GAGTC 2 cut(s) 561, 1831
PpsI GAGTC 2 cut(s) 561, 1831
Ppu21I YACGTR 1 cut(s) 968
PpuMI RGGWCCY 2 cut(s) 728, 2172
PscI ACATGT 1 cut(s) 1206
Psp1406I AACGTT 1 cut(s) 1809
Psp5II RGGWCCY 2 cut(s) 728, 2172
Psp6I CCWGG 2 cut(s) 730, 1038
PspEI GGTNACC 1 cut(s) 1131
PspGI CCWGG 2 cut(s) 730, 1038
PspN4I GGNNCC 7 cut(s) 7, 123, 1127, 1167, 2113, 2219, 2266
PspPI GGNCC 7 cut(s) 261, 289, 534, 728, 1461, 2111, 2172
PspPPI RGGWCCY 2 cut(s) 728, 2172
PstNI CAGNNNCTG 1 cut(s) 1904
PsuI RGATCY 3 cut(s) 5, 25, 569
PvuII CAGCTG 1 cut(s) 68
RsaI GTAC 8 cut(s) 136, 324, 1057, 1127, 1167, 1514, 1694, 2180
RsaNI GTAC 8 cut(s) 135, 323, 1056, 1126, 1166, 1513, 1693, 2179
RseI CAYNNNNRTG 3 cut(s) 152, 216, 2223
SatI GCNGC 5 cut(s) 333, 674, 814, 1419, 1545
Sau3AI GATC 8 cut(s) 5, 25, 538, 569, 867, 1268, 1531, 1548
Sau96I GGNCC 7 cut(s) 261, 289, 534, 728, 1461, 2111, 2172
ScaI AGTACT 1 cut(s) 136
SchI GAGTC 2 cut(s) 562, 1831
ScrFI CCNGG 3 cut(s) 732, 855, 1040
SduI GDGCHC 2 cut(s) 1214, 2220
SfaNI GCATC 7 cut(s) 635, 967, 1304, 1335, 1767, 2197, 2237
SfcI CTRYAG 1 cut(s) 1192
SfuI TTCGAA 1 cut(s) 1117
SinI GGWCC 3 cut(s) 261, 728, 2172
SmiMI CAYNNNNRTG 3 cut(s) 152, 216, 2223
SmlI CTYRAG 1 cut(s) 1401
SmoI CTYRAG 1 cut(s) 1401
SphI GCATGC 1 cut(s) 1261
SsiI CCGC 3 cut(s) 573, 795, 1586
SspI AATATT 1 cut(s) 681
SspMI CTAG 3 cut(s) 405, 1682, 2031
StyD4I CCNGG 3 cut(s) 730, 853, 1038
StyI CCWWGG 5 cut(s) 513, 542, 1104, 2144, 2268
TaaI ACNGT 9 cut(s) 151, 188, 256, 719, 1055, 1996, 2189, 2245, 2264
TaiI ACGT 4 cut(s) 970, 999, 1016, 1812
TaqI TCGA 9 cut(s) 474, 521, 589, 654, 721, 1117, 2076, 2123, 2205
TatI WGTACW 5 cut(s) 134, 322, 1055, 1512, 1692
TfiI GAWTC 5 cut(s) 1062, 1379, 1428, 2034, 2193
TscAI CASTG 4 cut(s) 1006, 1429, 1738, 2230
TseFI GTSAC 4 cut(s) 170, 997, 1131, 1348
TseI GCWGC 5 cut(s) 332, 673, 813, 1418, 1544
Tsp45I GTSAC 4 cut(s) 170, 997, 1131, 1348
TspDTI ATGAA 4 cut(s) 773, 876, 1263, 1646
TspGWI ACGGA 1 cut(s) 389
TspRI CASTG 4 cut(s) 1006, 1429, 1738, 2230
VpaK11BI GGWCC 3 cut(s) 261, 728, 2172
XagI CCTNNNNNAGG 1 cut(s) 828
XapI RAATTY 3 cut(s) 780, 1289, 1720
XbaI TCTAGA 1 cut(s) 2030
XceI RCATGY 2 cut(s) 1210, 1261
XmnI GAANNNNTTC 1 cut(s) 762
XspI CTAG 3 cut(s) 405, 1682, 2031
ZraI GACGTC 1 cut(s) 997
ZrmI AGTACT 1 cut(s) 136
Zsp2I ATGCAT 2 cut(s) 1942, 2212
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.