Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr6C

Orthogroup ID

OG0004846

Physical Location & Seq

Genomic Coordinates Forward (+)

65966632 .. 65969650

Gene Structure 3019 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh6CG493700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1740 bp

ATGGACAGGGTTCCGTTTCTGTGCCTTGTTTTTGTTTTTGCACTAATTTTTCCATTCAAATCATCAACAGCTTTTAATTTGAGCATGGGATCCTTTCTTTCAGTAGAAAATCCATCTCAAACTTTGATGTCTCCGGACGCAGTATTTACCGCTGGCTTTTTTCCGGTCGGTGAAAATGCGTTTTGCTTCGGAATATGGTTCTCGGAGCCATCAACCAGTACTCACAACCTCACCGTGGTGTGGATAGCAAATCGTGACGAGCCGGTCAACAGTAAACGCTCTAAGCTCACCCTGCACAAAAGTGGAAACCTTATTCTCTCCGACATCGACCGATACACCGTGTGGTCCTCCAACACAGCTTCAAACTCATCGGCCCACTTGCACCTTAGAAACACCGGCAATCTTGTTCTGCTCGCTGCGAACGGCGGCGGCGTATTGTGGGAAAGCTTTGCTTCGCCGACGGACACACTTTTACCTCAGCAAGCGCTCACTAGAAACACACAGCTCATTTCTTCAAGAAGCCTCACCAACTATTCCTCCGGCTTCTACTCTTTTTACTTCGACAACGACAACACTCTTCGTATGCTCTACAACGGCCGCGAGGTTTCAAGCATTTACTGGCCTGATCCATGGCTTCTGAGTTGGCAAGTAGGAAGATCCACATACAACAACAGTCGAATTGCAACTTTTGACAGCTTGGGAAATTTTAGTGCATCTGATAATTTTATCGTTTGGACAATCGACTACGGTGCAAATTTGCAGCGAATATTCAGGATGGATGTAGATGGTAATCTCCGAGGTTACAGTCGAAAAGGTCCTGGAGATAAATGGATTGTTACATGGGAGGCAGTTTTACAACCTTGTAAAATTCATGGCATTTGCGGAGCTAATAGCTATTGCAGCTATATTCCTAGTACAGGTAGGAAATGCTCATGCCTGCCGGGTTATGAAATGATCAGTAAAACCGATTGGTCTTATGGGTGTAAACCGGCTGGTTTCAATCTGTCCTGCAATAGTGCACAGGAGGAGTTTGGTTTTGTGCATCTTCCACGTATTGAGATGTATGGTTATGATTATGCCGTCTATGGCAATTACACATTAGATGAGTGCAAAAGTTTGTGCCTGGACTTGTGCAACTGTTCTGCATTCCACCACAAATTTGGTGGTGGTCAGTATCAGTGTTATCCCAAGGCGCAATTACGAAATGGGTACCGGTCACCTGCTTTTGATGGGGAGCTGTATGTCAAGCTACCAAAAGGCCTCCTCAATGCTACCTATAGAGATACCAACATGTGCTCAGCTAAACTTACAGCACAGCTTGACAGAACATATGAAAGAAGCAAGCCCAATGGATCGATTGAGTTTATGCTGAAATTTGCATCTGCCTTGGGAGGAATAGAGGTTATTTGCATCGTTGTTGTGTGGTTTTTTGTGACAAGAAGAAGCGGTGGAGATAAAAAAGATTCAGAATTAGAACTACTTGCTCAAGGCTACCTTCAAGCTGCCAGTGGATTCAGAAGGTTTACTTACTCCGAGCTGAAGAAGGCCACAAAAGGTTTTAAAGAAGAGATTGGCAGAGGAGGAGGAGGAGTTGTGTACAAAGGCGTATTGTCTGATCAACGAGTTGCAGCGATCAAGCAACTGAATGATTGTAGACAAGGGGGTGAAGCAGAGTTCCTTGCAGAAGGTTTCTGGGTCGGACTCAACTTTGATCACCATCCTGGGTCTCTCGGATCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0016020

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

B_lectin PAN_1 PAN_2 Pkinase S_locus_glycop

Protein Analysis

579

Amino Acids

64.54

Weight (kDa)

8.2

Isoelectric Point (pI)

36.18

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
B_lectin	PF01453	78 - 176	3.8e-25	D-mannose binding lectin
S_locus_glycop	PF00954	244 - 317	3.3e-07	S-locus glycoprotein domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004846)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g33830 FvH4_2g33840 FvH4_2g33850
malus_domestica	MD15G1076500.v1.1
prunus_persica	Prupe.1G429800_v2.0.a1 Prupe.1G429900_v2.0.a1
pyrus_communis	pycom08g07440
rosa_chinensis	RchiOBHm_Chr6g0308701 RchiOBHm_Chr6g0308721
rosa_laevigata	RLG00000010627 RLG00000010628
rosa_multiflora	Rmu_co8093658.1_g000001 Rmu_sc0000871.1_g000016 Rmu_sc0000871.1_g000018 Rmu_sc0017014.1_g000001 Rmu_sc0017015.1_g000001
rosa_roxburghii	Rroxscaffold_7G00160000 Rroxscaffold_7G00160010
rosa_rugosa	Rorug06G0367800
rosa_samantha	Rh6AG478400 Rh6AG478800 Rh6BG488300 Rh6BG488400 Rh6CG492900 Rh6CG493200 Rh6CG493400 Rh6CG493600 Rh6CG493700 Rh6DG479200 Rh6DG479300
rosa_wichuraiana	Rw6G041720

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1228
AasI	GACNNNNNNGTC	1 cut(s)	263
Acc36I	ACCTGC	1 cut(s)	1228
Acc65I	GGTACC	1 cut(s)	1209
AccB1I	GGYRCC	1 cut(s)	1209
AccI	GTMKAC	1 cut(s)	1654
AccII	CGCG	1 cut(s)	600
AccIII	TCCGGA	1 cut(s)	133
AciI	CCGC	6 cut(s)	150, 426, 429, 598, 882, 1446
AclWI	GGATC	5 cut(s)	84, 97, 620, 651, 1360
AcoI	YGGCCR	1 cut(s)	595
AcsI	RAATTY	5 cut(s)	703, 754, 867, 1157, 1373
AcuI	CTGAAG	1 cut(s)	1559
AdeI	CACNNNGTG	1 cut(s)	342
AfaI	GTAC	4 cut(s)	220, 916, 1211, 1598
AfeI	AGCGCT	1 cut(s)	486
AfiI	CCNNNNNNNGG	3 cut(s)	235, 240, 917
AflIII	ACRYGT	1 cut(s)	1290
AgeI	ACCGGT	1 cut(s)	1212
AgsI	TTSAA	6 cut(s)	58, 363, 516, 609, 1000, 1499
AjnI	CCWGG	3 cut(s)	817, 1122, 1720
AleI	CACNNNNGTG	2 cut(s)	236, 300
Alw21I	GWGCWC	2 cut(s)	1021, 1298
Alw26I	GTCTC	2 cut(s)	135, 1731
Alw44I	GTGCAC	1 cut(s)	1017
AlwI	GGATC	5 cut(s)	84, 97, 620, 651, 1360
Aor13HI	TCCGGA	1 cut(s)	133
Aor51HI	AGCGCT	1 cut(s)	486
AoxI	GGCC	5 cut(s)	372, 595, 620, 1258, 1545
ApaLI	GTGCAC	1 cut(s)	1017
ApeKI	GCWGC	5 cut(s)	416, 760, 900, 1502, 1628
ApoI	RAATTY	5 cut(s)	703, 754, 867, 1157, 1373
AsiGI	ACCGGT	1 cut(s)	1212
Asp718I	GGTACC	1 cut(s)	1209
AspLEI	GCGC	2 cut(s)	487, 1195
AspS9I	GGNCC	3 cut(s)	345, 373, 815
AsuC2I	CCSGG	1 cut(s)	942
AsuHPI	GGTGA	7 cut(s)	182, 223, 280, 517, 1209, 1676, 1706
AvaII	GGWCC	2 cut(s)	345, 815
BaeGI	GKGCMC	1 cut(s)	1021
BamHI	GGATCC	1 cut(s)	89
BanI	GGYRCC	1 cut(s)	1209
Bbv12I	GWGCWC	2 cut(s)	1021, 1298
BbvCI	CCTCAGC	1 cut(s)	477
BbvI	GCAGC	5 cut(s)	403, 772, 912, 1489, 1640
BccI	CCATC	6 cut(s)	121, 217, 769, 779, 1223, 1725
BceAI	ACGGC	3 cut(s)	439, 610, 1064
BcgI	CGANNNNNNTGC	2 cut(s)	731, 765
BciT130I	CCWGG	3 cut(s)	819, 1124, 1722
BclI	TGATCA	3 cut(s)	954, 1615, 1711
BcnI	CCSGG	1 cut(s)	942
BcoDI	GTCTC	2 cut(s)	135, 1731
BfaI	CTAG	2 cut(s)	492, 912
BfmI	CTRYAG	1 cut(s)	1276
BfoI	RGCGCY	1 cut(s)	488
BfuAI	ACCTGC	1 cut(s)	1228
BisI	GCNGC	8 cut(s)	417, 427, 430, 598, 761, 901, 1503, 1629
BlpI	GCTNAGC	1 cut(s)	1297
BlsI	GCNGC	8 cut(s)	418, 428, 431, 599, 762, 902, 1504, 1630
BmcAI	AGTACT	1 cut(s)	220
Bme1390I	CCNGG	4 cut(s)	819, 942, 1124, 1722
Bme18I	GGWCC	2 cut(s)	345, 815
BmgT120I	GGNCC	3 cut(s)	345, 373, 815
BmiI	GGNNCC	4 cut(s)	12, 91, 207, 1211
BmrFI	CCNGG	4 cut(s)	819, 942, 1124, 1722
BmsI	GCATC	4 cut(s)	722, 1051, 1388, 1419
BpmI	CTGGAG	1 cut(s)	840
Bpu10I	CCTNAGC	1 cut(s)	477
Bpu1102I	GCTNAGC	1 cut(s)	1297
BpuEI	CTTGAG	1 cut(s)	1470
BpuMI	CCSGG	1 cut(s)	942
Bsa29I	ATCGAT	1 cut(s)	1355
BsaAI	YACGTR	1 cut(s)	1052
BsaBI	GATNNNNATC	2 cut(s)	789, 1716
BsaI	GGTCTC	1 cut(s)	1731
BsaJI	CCNNGG	6 cut(s)	234, 629, 796, 1188, 1386, 1721
BsaWI	WCCGGW	3 cut(s)	133, 163, 1212
BsaXI	ACNNNNNCTCC	6 cut(s)	521, 551, 1575, 1578, 1605, 1608
Bsc4I	CCNNNNNNNGG	3 cut(s)	235, 240, 917
Bse118I	RCCGGY	4 cut(s)	262, 395, 988, 1212
Bse1I	ACTGG	3 cut(s)	216, 623, 1506
Bse8I	GATNNNNATC	2 cut(s)	789, 1716
BseAI	TCCGGA	1 cut(s)	133
BseBI	CCWGG	3 cut(s)	819, 1124, 1722
BseCI	ATCGAT	1 cut(s)	1355
BseDI	CCNNGG	6 cut(s)	234, 629, 796, 1188, 1386, 1721
BseGI	GGATG	3 cut(s)	780, 784, 1717
BseJI	GATNNNNATC	2 cut(s)	789, 1716
BseLI	CCNNNNNNNGG	3 cut(s)	235, 240, 917
BseMII	CTCAG	3 cut(s)	491, 629, 1311
BseNI	ACTGG	3 cut(s)	216, 623, 1506
BseRI	GAGGAG	6 cut(s)	1040, 1253, 1593, 1596, 1599, 1602
BseSI	GKGCMC	1 cut(s)	1021
BseX3I	CGGCCG	1 cut(s)	595
BseXI	GCAGC	5 cut(s)	403, 772, 912, 1489, 1640
BsgI	GTGCAG	1 cut(s)	278
Bsh1236I	CGCG	1 cut(s)	600
Bsh1285I	CGRYCG	3 cut(s)	168, 331, 598
BshFI	GGCC	5 cut(s)	374, 597, 622, 1260, 1547
BshNI	GGYRCC	1 cut(s)	1209
BshTI	ACCGGT	1 cut(s)	1212
BshVI	ATCGAT	1 cut(s)	1355
BsiEI	CGRYCG	3 cut(s)	168, 331, 598
BsiHKAI	GWGCWC	2 cut(s)	1021, 1298
BsiSI	CCGG	8 cut(s)	134, 164, 263, 396, 540, 941, 989, 1213
BslI	CCNNNNNNNGG	3 cut(s)	235, 240, 917
BsmAI	GTCTC	2 cut(s)	135, 1731
BsmI	GAATGC	1 cut(s)	1145
BsnI	GGCC	5 cut(s)	374, 597, 622, 1260, 1547
Bso31I	GGTCTC	1 cut(s)	1731
Bsp1286I	GDGCHC	2 cut(s)	1021, 1298
Bsp13I	TCCGGA	1 cut(s)	133
Bsp1407I	TGTACA	1 cut(s)	1596
Bsp143I	GATC	9 cut(s)	89, 625, 656, 954, 1352, 1615, 1632, 1711, 1733
Bsp1720I	GCTNAGC	1 cut(s)	1297
Bsp19I	CCATGG	1 cut(s)	629
BspACI	CCGC	6 cut(s)	150, 426, 429, 598, 882, 1446
BspANI	GGCC	5 cut(s)	374, 597, 622, 1260, 1547
BspCNI	CTCAG	3 cut(s)	490, 630, 1310
BspDI	ATCGAT	1 cut(s)	1355
BspEI	TCCGGA	1 cut(s)	133
BspFNI	CGCG	1 cut(s)	600
BspLI	GGNNCC	4 cut(s)	12, 91, 207, 1211
BspMI	ACCTGC	1 cut(s)	1228
BspPI	GGATC	5 cut(s)	84, 97, 620, 651, 1360
BspT107I	GGYRCC	1 cut(s)	1209
BspTNI	GGTCTC	1 cut(s)	1731
BsrFI	RCCGGY	4 cut(s)	262, 395, 988, 1212
BsrGI	TGTACA	1 cut(s)	1596
BsrI	ACTGG	3 cut(s)	216, 623, 1506
BssAI	RCCGGY	4 cut(s)	262, 395, 988, 1212
BssECI	CCNNGG	6 cut(s)	234, 629, 796, 1188, 1386, 1721
BssMI	GATC	9 cut(s)	89, 625, 656, 954, 1352, 1615, 1632, 1711, 1733
BssT1I	CCWWGG	3 cut(s)	629, 1188, 1386
Bst2UI	CCWGG	3 cut(s)	819, 1124, 1722
Bst4CI	ACNGT	7 cut(s)	235, 272, 340, 674, 749, 806, 1139
Bst6I	CTCTTC	2 cut(s)	582, 1560
BstAUI	TGTACA	1 cut(s)	1596
BstBAI	YACGTR	1 cut(s)	1052
BstC8I	GCNNGC	5 cut(s)	154, 414, 483, 938, 1343
BstDEI	CTNAG	5 cut(s)	282, 386, 477, 638, 1297
BstDSI	CCRYGG	2 cut(s)	234, 629
BstEII	GGTNACC	1 cut(s)	1215
BstENI	CCTNNNNNAGG	1 cut(s)	915
BstF5I	GGATG	3 cut(s)	780, 784, 1717
BstFNI	CGCG	1 cut(s)	600
BstH2I	RGCGCY	1 cut(s)	488
BstHHI	GCGC	2 cut(s)	487, 1195
BstKTI	GATC	9 cut(s)	92, 628, 659, 957, 1355, 1618, 1635, 1714, 1736
BstMAI	GTCTC	2 cut(s)	135, 1731
BstMBI	GATC	9 cut(s)	89, 625, 656, 954, 1352, 1615, 1632, 1711, 1733
BstMCI	CGRYCG	3 cut(s)	168, 331, 598
BstMWI	GCNNNNNNNGC	2 cut(s)	292, 900
BstNI	CCWGG	3 cut(s)	819, 1124, 1722
BstNSI	RCATGY	1 cut(s)	1294
BstPI	GGTNACC	1 cut(s)	1215
BstSCI	CCNGG	4 cut(s)	817, 940, 1122, 1720
BstSFI	CTRYAG	1 cut(s)	1276
BstSLI	GKGCMC	1 cut(s)	1021
BstUI	CGCG	1 cut(s)	600
BstV1I	GCAGC	5 cut(s)	403, 772, 912, 1489, 1640
BstX2I	RGATCY	2 cut(s)	89, 656
BstXI	CCANNNNNNTGG	1 cut(s)	1160
BstYI	RGATCY	2 cut(s)	89, 656
BstZI	CGGCCG	1 cut(s)	595
Bsu15I	ATCGAT	1 cut(s)	1355
BsuRI	GGCC	5 cut(s)	374, 597, 622, 1260, 1547
BsuTUI	ATCGAT	1 cut(s)	1355
BtgI	CCRYGG	2 cut(s)	234, 629
BtsCI	GGATG	3 cut(s)	780, 784, 1717
BtsIMutI	CAGTG	2 cut(s)	1184, 1513
BveI	ACCTGC	1 cut(s)	1228
Cac8I	GCNNGC	5 cut(s)	154, 414, 483, 938, 1343
CfoI	GCGC	2 cut(s)	487, 1195
Cfr10I	RCCGGY	4 cut(s)	262, 395, 988, 1212
Cfr13I	GGNCC	3 cut(s)	345, 373, 815
ClaI	ATCGAT	1 cut(s)	1355
CseI	GACGC	1 cut(s)	146
Csp6I	GTAC	4 cut(s)	219, 915, 1210, 1597
CspAI	ACCGGT	1 cut(s)	1212
CspCI	CAANNNNNGTGG	2 cut(s)	1144, 1179
CviAII	CATG	6 cut(s)	85, 630, 840, 872, 933, 1291
CviQI	GTAC	4 cut(s)	219, 915, 1210, 1597
DdeI	CTNAG	5 cut(s)	282, 386, 477, 638, 1297
DpnI	GATC	9 cut(s)	91, 627, 658, 956, 1354, 1617, 1634, 1713, 1735
DpnII	GATC	9 cut(s)	89, 625, 656, 954, 1352, 1615, 1632, 1711, 1733
DraI	TTTAAA	1 cut(s)	1561
DraIII	CACNNNGTG	1 cut(s)	342
DrdI	GACNNNNNNGTC	1 cut(s)	263
DseDI	GACNNNNNNGTC	1 cut(s)	263
EaeI	YGGCCR	1 cut(s)	595
EagI	CGGCCG	1 cut(s)	595
Eam1104I	CTCTTC	2 cut(s)	582, 1560
EarI	CTCTTC	2 cut(s)	582, 1560
EclXI	CGGCCG	1 cut(s)	595
Eco130I	CCWWGG	3 cut(s)	629, 1188, 1386
Eco147I	AGGCCT	1 cut(s)	1260
Eco31I	GGTCTC	1 cut(s)	1731
Eco47I	GGWCC	2 cut(s)	345, 815
Eco47III	AGCGCT	1 cut(s)	486
Eco52I	CGGCCG	1 cut(s)	595
Eco57I	CTGAAG	1 cut(s)	1559
Eco91I	GGTNACC	1 cut(s)	1215
EcoNI	CCTNNNNNAGG	1 cut(s)	915
EcoO109I	RGGNCCY	1 cut(s)	815
EcoO65I	GGTNACC	1 cut(s)	1215
EcoRII	CCWGG	3 cut(s)	817, 1122, 1720
EcoT14I	CCWWGG	3 cut(s)	629, 1188, 1386
ErhI	CCWWGG	3 cut(s)	629, 1188, 1386
FaeI	CATG	6 cut(s)	88, 633, 843, 875, 936, 1294
FalI	AAGNNNNNCTT	6 cut(s)	436, 468, 1479, 1511, 1510, 1542
FatI	CATG	6 cut(s)	84, 629, 839, 871, 932, 1290
FauNDI	CATATG	1 cut(s)	1330
FbaI	TGATCA	3 cut(s)	954, 1615, 1711
FblI	GTMKAC	1 cut(s)	1654
Fnu4HI	GCNGC	8 cut(s)	417, 427, 430, 598, 761, 901, 1503, 1629
FokI	GGATG	3 cut(s)	787, 791, 1704
Fsp4HI	GCNGC	8 cut(s)	417, 427, 430, 598, 761, 901, 1503, 1629
FspBI	CTAG	2 cut(s)	492, 912
GlaI	GCGC	2 cut(s)	486, 1194
GluI	GCNGC	8 cut(s)	417, 427, 430, 598, 761, 901, 1503, 1629
GsuI	CTGGAG	1 cut(s)	840
HaeII	RGCGCY	1 cut(s)	488
HaeIII	GGCC	5 cut(s)	374, 597, 622, 1260, 1547
HapII	CCGG	8 cut(s)	134, 164, 263, 396, 540, 941, 989, 1213
HgaI	GACGC	1 cut(s)	146
HhaI	GCGC	2 cut(s)	487, 1195
Hin1II	CATG	6 cut(s)	88, 633, 843, 875, 936, 1294
Hin6I	GCGC	2 cut(s)	485, 1193
HinP1I	GCGC	2 cut(s)	485, 1193
HincII	GTYRAC	1 cut(s)	268
HindII	GTYRAC	1 cut(s)	268
HindIII	AAGCTT	1 cut(s)	445
HinfI	GANTC	3 cut(s)	1463, 1512, 1701
HpaII	CCGG	8 cut(s)	134, 164, 263, 396, 540, 941, 989, 1213
HphI	GGTGA	7 cut(s)	182, 223, 280, 517, 1209, 1676, 1706
Hpy166II	GTNNAC	7 cut(s)	268, 275, 986, 1019, 1524, 1597, 1655
Hpy188III	TCNNGA	4 cut(s)	134, 254, 516, 772
Hpy8I	GTNNAC	7 cut(s)	268, 275, 986, 1019, 1524, 1597, 1655
Hpy99I	CGWCG	1 cut(s)	463
HpyAV	CCTTC	4 cut(s)	1505, 1512, 1537, 1679
HpyCH4III	ACNGT	7 cut(s)	235, 272, 340, 674, 749, 806, 1139
HpyCH4IV	ACGT	1 cut(s)	1051
HpyF10VI	GCNNNNNNNGC	2 cut(s)	292, 900
HpyF3I	CTNAG	5 cut(s)	282, 386, 477, 638, 1297
HpySE526I	ACGT	1 cut(s)	1051
Hsp92II	CATG	6 cut(s)	88, 633, 843, 875, 936, 1294
HspAI	GCGC	2 cut(s)	485, 1193
Kpn2I	TCCGGA	1 cut(s)	133
KpnI	GGTACC	1 cut(s)	1213
Ksp22I	TGATCA	3 cut(s)	954, 1615, 1711
Kzo9I	GATC	9 cut(s)	89, 625, 656, 954, 1352, 1615, 1632, 1711, 1733
LmnI	GCTCC	3 cut(s)	205, 884, 1234
Lsp1109I	GCAGC	5 cut(s)	403, 772, 912, 1489, 1640
LweI	GCATC	4 cut(s)	722, 1051, 1388, 1419
MaeI	CTAG	2 cut(s)	492, 912
MaeII	ACGT	1 cut(s)	1051
MaeIII	GTNAC	5 cut(s)	254, 800, 835, 1215, 1432
MalI	GATC	9 cut(s)	91, 627, 658, 956, 1354, 1617, 1634, 1713, 1735
MboI	GATC	9 cut(s)	89, 625, 656, 954, 1352, 1615, 1632, 1711, 1733
MboII	GAAGA	7 cut(s)	504, 569, 666, 1037, 1452, 1552, 1577
MflI	RGATCY	2 cut(s)	89, 656
MhlI	GDGCHC	2 cut(s)	1021, 1298
MlyI	GAGTC	1 cut(s)	1695
MmeI	TCCRAC	3 cut(s)	345, 375, 1678
MroI	TCCGGA	1 cut(s)	133
MseI	TTAA	2 cut(s)	75, 1560
MslI	CAYNNNNRTG	2 cut(s)	236, 300
MspA1I	CMGCKG	1 cut(s)	152
MspI	CCGG	8 cut(s)	134, 164, 263, 396, 540, 941, 989, 1213
MspR9I	CCNGG	4 cut(s)	819, 942, 1124, 1722
Mva1269I	GAATGC	1 cut(s)	1145
MvaI	CCWGG	3 cut(s)	819, 1124, 1722
MvnI	CGCG	1 cut(s)	600
MwoI	GCNNNNNNNGC	2 cut(s)	292, 900
NciI	CCSGG	1 cut(s)	942
NcoI	CCATGG	1 cut(s)	629
NdeI	CATATG	1 cut(s)	1330
NdeII	GATC	9 cut(s)	89, 625, 656, 954, 1352, 1615, 1632, 1711, 1733
NlaIII	CATG	6 cut(s)	88, 633, 843, 875, 936, 1294
NlaIV	GGNNCC	4 cut(s)	12, 91, 207, 1211
NmuCI	GTSAC	3 cut(s)	254, 1215, 1432
NspI	RCATGY	1 cut(s)	1294
OliI	CACNNNNGTG	2 cut(s)	236, 300
PaqCI	CACCTGC	1 cut(s)	1228
PceI	AGGCCT	1 cut(s)	1260
PciI	ACATGT	1 cut(s)	1290
PcsI	WCGNNNNNNNCGW	1 cut(s)	429
PctI	GAATGC	1 cut(s)	1145
PfeI	GAWTC	2 cut(s)	1463, 1512
PfoI	TCCNGGA	1 cut(s)	817
PinAI	ACCGGT	1 cut(s)	1212
PkrI	GCNGC	8 cut(s)	418, 428, 431, 599, 762, 902, 1504, 1630
PleI	GAGTC	1 cut(s)	1695
PpsI	GAGTC	1 cut(s)	1695
Ppu21I	YACGTR	1 cut(s)	1052
PpuMI	RGGWCCY	1 cut(s)	815
PscI	ACATGT	1 cut(s)	1290
Psp5II	RGGWCCY	1 cut(s)	815
Psp6I	CCWGG	3 cut(s)	817, 1122, 1720
PspEI	GGTNACC	1 cut(s)	1215
PspGI	CCWGG	3 cut(s)	817, 1122, 1720
PspN4I	GGNNCC	4 cut(s)	12, 91, 207, 1211
PspPI	GGNCC	3 cut(s)	345, 373, 815
PspPPI	RGGWCCY	1 cut(s)	815
PsuI	RGATCY	2 cut(s)	89, 656
RsaI	GTAC	4 cut(s)	220, 916, 1211, 1598
RsaNI	GTAC	4 cut(s)	219, 915, 1210, 1597
RseI	CAYNNNNRTG	2 cut(s)	236, 300
SaqAI	TTAA	2 cut(s)	75, 1560
SatI	GCNGC	8 cut(s)	417, 427, 430, 598, 761, 901, 1503, 1629
Sau3AI	GATC	9 cut(s)	89, 625, 656, 954, 1352, 1615, 1632, 1711, 1733
Sau96I	GGNCC	3 cut(s)	345, 373, 815
ScaI	AGTACT	1 cut(s)	220
SchI	GAGTC	1 cut(s)	1695
ScrFI	CCNGG	4 cut(s)	819, 942, 1124, 1722
SduI	GDGCHC	2 cut(s)	1021, 1298
SfaNI	GCATC	4 cut(s)	722, 1051, 1388, 1419
SfcI	CTRYAG	1 cut(s)	1276
SinI	GGWCC	2 cut(s)	345, 815
SmiMI	CAYNNNNRTG	2 cut(s)	236, 300
SmlI	CTYRAG	1 cut(s)	1485
SmoI	CTYRAG	1 cut(s)	1485
SseBI	AGGCCT	1 cut(s)	1260
SsiI	CCGC	6 cut(s)	150, 426, 429, 598, 882, 1446
SspI	AATATT	1 cut(s)	768
SspMI	CTAG	2 cut(s)	492, 912
StuI	AGGCCT	1 cut(s)	1260
StyD4I	CCNGG	4 cut(s)	817, 940, 1122, 1720
StyI	CCWWGG	3 cut(s)	629, 1188, 1386
TaaI	ACNGT	7 cut(s)	235, 272, 340, 674, 749, 806, 1139
TaiI	ACGT	1 cut(s)	1054
TaqI	TCGA	6 cut(s)	327, 561, 676, 741, 808, 1355
TaqII	GACCGA	1 cut(s)	345
TatI	WGTACW	3 cut(s)	218, 914, 1596
TauI	GCSGC	3 cut(s)	429, 432, 600
TfiI	GAWTC	2 cut(s)	1463, 1512
Tru1I	TTAA	2 cut(s)	75, 1560
Tru9I	TTAA	2 cut(s)	75, 1560
TscAI	CASTG	2 cut(s)	1184, 1513
TseFI	GTSAC	3 cut(s)	254, 1215, 1432
TseI	GCWGC	5 cut(s)	416, 760, 900, 1502, 1628
Tsp45I	GTSAC	3 cut(s)	254, 1215, 1432
TspDTI	ATGAA	3 cut(s)	860, 963, 1347
TspGWI	ACGGA	1 cut(s)	476
TspRI	CASTG	2 cut(s)	1184, 1513
VneI	GTGCAC	1 cut(s)	1017
VpaK11BI	GGWCC	2 cut(s)	345, 815
XagI	CCTNNNNNAGG	1 cut(s)	915
XapI	RAATTY	5 cut(s)	703, 754, 867, 1157, 1373
XceI	RCATGY	1 cut(s)	1294
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1157, 1160
XmiI	GTMKAC	1 cut(s)	1654
XspI	CTAG	2 cut(s)	492, 912
ZrmI	AGTACT	1 cut(s)	220

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.