MD15G1076500.v1.1

receptor protein kinase ZmPK1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0004846
Physical Location & Seq
Forward (+)
5192453 .. 5194837
2385 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1076500.v1.1.491

Sequence Viewer

Length: 2385 bp
ATGGCTTCTACATTTTTGTGCATTGTTCTTCTTTTTCTAGCACAATCTTCCCCGTTGGCATCATCAGCGTCCAATAATCTCAAACCCGGATCGTCTCTCTCGGTCGAAAATTCAGCCGACAAATTGATCTCAACAAATGGCATGTTTTCAGCTGGATTTTTTCCGGTGGGTGAAAATGCGTATTGTTTCGGGATATGGTTCACCGAGCCAGCAGCAGCCGACGCCGCCACTCAAAACCTCACCGTCGTTTGGACGGCCAACCGCGACCAGCTGGTCAACGGTAGGGGATCCAAGTTCACCCTACAAAAAAACGGCAACCTTATTCTAACCGATGCTGGAAAATATACCATTTGGTCCTCCAACACTGTTTTTTCTAATTCTCTGGCCCAGTTAATTCTCGAAGACAACGGAAATCTTGTACTTCTCACTCTCAAGGGTGTTGCTTTGTGGGAAAGCTTTGCTTCCCCCACAGACACACTTTTACCACAGCAGCAACTTACTAGGAACACAAAGCTTGTCTCTTCAAGAAGCCTCACTAACTGTTCATCCGGGTTCTACTCTCTATTTTTCGACAATGATAACACGCTTCGGCTTCTTTACGACGGCCCAGAGGTTTCGAGCATATACTGGCATGATCCTTGGCTGCTAAGTTTGCAAGCTGGAAGGACAACTTACAACAACTCTAGGGTTGCAGTGCTAGATGCTTTGGGGAATTTCAGTGCATCGGATGGACTTAATTTTATGTCATCAGATTATGGCGTGAAGTTGCAGCGAAGAGTGAAGGCGGATGTTGATGGTAATCTCCGATTGTACAGTCGAAAAAGCCCGGGAGATAGATGGGTTGTTTCATGGCAAGCAATTGCTGAACCCTGCAAAATTCATGGCATTTGTGGGCCTAATGGTTTGTGCACGTATGAGCCTAGTTCGGGTCGAAAATGCTCGTGCCTTCCAGGCTACGAAATGAGAAACAAAAACGACTGGGCTTATGGATGCCAGCCTACTACAGAAGGTTTCAATGTGTCTTGTAAAAGTAGTACAGCTCGAGATCAGTTCGACTTTATGTATCTTCCGAGGGTTGAATATTTCGGGTACGATTACTTCATTTACGTTAACACGTCATTAGAAAAGTGCAAAGCTATATGCTTAGAGTTATGCAACTGTCCAGGTTTCCACTACAAGTTTGGAGGTGGCCATCACAATTGTTATCCCAAGACTCAATTTCGAAACGGTTATCGCACGCCTGGTTTTACAGGAGAGCTGTATCTGAAGCTGCCCAAATCCATCCTTTCTTCGTATAATGCTACTGCAAAAGGATTCAACATGTGCTCGAGCAAACTCACAAGCCAGCTTTCCAGAACATATGAGAAAAAAAGTACAAATGGTTCCGTCAATTTTATGCTGAAATTTGCATCTGCATTGGGGGGTCTTGAACTTATCTGTATCATTGTTGTGTGGTATTTTGTGATGCGAAGCAGCGAAGAGAAAGAAGAGTCGGATGTGGTTACACAAGGCTACCTTCATGCTGTAACCGGTTTCAGAAGATTTAGCTACTCCGAGTTGAAAAAGGCGACACGAGGTTTCAAAGAAGAAATTGGGAGAGGTGGGGGAGGAATTGTGTACAAAGGCACATTGTCTGATCAACGCGTCGCAGCAATCAAGCAGTTGAATGCTGCAACCCAAGGGGAAGCAGAGTTTTTAGCGGAAGTGAGCCTGATTGGGAAGCTTTACCACATGAACTTGATCGAAATGTGGGGCTATTGCGTGGAAGGCAAGCACAGGCTTCTGGTTTATGAGTACATGAAGAATGGTTCCTTGGCTGAGAAGTTATCTTCCAATGTGCTCGATTGGAATCAAAAGTTCGAAATTGCTGTGGGGACAGCAAAAGGGCTGGCTTATTTGCACGAAGAATGCTTGGAATGGGTACTACATTGTGATGTGAAGCCTCAAAACATACTCTTGGACTCGAACTATAACCCGAAAGTGGCGGATTTTGGCTTGTCCAAGCTTTTAAACCGAAGTGAAGTCAGCAACTCGAAGTTTTCGAGGATACGAGGAACAAGAGGCTACATGGCACCGGAGTGGATTCAGAACATGCCCATCACTTCTAAAGTGGATGTTTACAGCTATGGGATTGTTGTATTGGAGATGGTGACCGGAAAAAGCCCGGCGGAAGGTGTTCAATCATCGGAAGGTGGAGGGGAGACGCAGAAGAAGATGATGGTTTCACGGGTGAGGGAGAACATGAACGGAGGATTTGAAAATACAGAGAGCAAGAAGGGAGAAGGGCTCATGAAAGTGGCTCTTCGGTGTTCGGAGGAAGACAGAGATGCAAGGCCTACTATGACGCAAGTAGTTGAGATGCTTCTACAGTATCAAGATGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0016020
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin PAN_1 PAN_2 Pkinase S_locus_glycop

Protein Analysis

795

Amino Acids

88.37

Weight (kDa)

8.19

Isoelectric Point (pI)

33.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 81 - 179 7.7e-21 D-mannose binding lectin
S_locus_glycop PF00954 257 - 322 3.8e-08 S-locus glycoprotein domain
PK_Tyr_Ser-Thr PF07714 527 - 788 1.2e-45 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 528 - 786 5.4e-54 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 272
AccB1I GGYRCC 1 cut(s) 2071
AccII CGCG 2 cut(s) 264, 1644
AciI CCGC 6 cut(s) 225, 262, 785, 1700, 1985, 2168
AclWI GGATC 4 cut(s) 97, 282, 295, 629
AcoI YGGCCR 2 cut(s) 255, 1189
AcsI RAATTY 4 cut(s) 109, 712, 876, 1403
AcuI CTGAAG 1 cut(s) 1286
AcyI GRCGYC 1 cut(s) 222
AfaI GTAC 8 cut(s) 420, 812, 1036, 1091, 1375, 1619, 1796, 1923
AfiI CCNNNNNNNGG 4 cut(s) 249, 926, 1981, 2171
AflIII ACRYGT 3 cut(s) 1113, 1320, 1642
AgeI ACCGGT 1 cut(s) 1529
AjiI CACGTC 1 cut(s) 1116
AjnI CCWGG 3 cut(s) 949, 1162, 1240
AjuI GAANNNNNNNTTGG 2 cut(s) 1796, 1828
AleI CACNNNNGTG 1 cut(s) 2077
Alw21I GWGCWC 3 cut(s) 911, 1328, 1842
Alw26I GTCTC 3 cut(s) 99, 523, 2195
Alw44I GTGCAC 1 cut(s) 907
AlwI GGATC 4 cut(s) 97, 282, 295, 629
Ama87I CYCGRG 3 cut(s) 826, 1041, 1327
AoxI GGCC 6 cut(s) 255, 384, 604, 893, 1189, 2333
ApaLI GTGCAC 1 cut(s) 907
ApeKI GCWGC 9 cut(s) 212, 215, 490, 643, 769, 1270, 1473, 1649, 1670
ApoI RAATTY 4 cut(s) 109, 712, 876, 1403
AsiGI ACCGGT 1 cut(s) 1529
Asp700I GAANNNNTTC 1 cut(s) 2175
AspS9I GGNCC 4 cut(s) 354, 385, 605, 893
AsuC2I CCSGG 5 cut(s) 87, 550, 827, 828, 2165
AsuHPI GGTGA 6 cut(s) 182, 193, 232, 289, 2161, 2242
AsuII TTCGAA 2 cut(s) 1222, 1860
AvaI CYCGRG 3 cut(s) 826, 1041, 1327
AvaII GGWCC 1 cut(s) 354
BaeGI GKGCMC 1 cut(s) 911
BalI TGGCCA 1 cut(s) 1191
BamHI GGATCC 1 cut(s) 287
BanI GGYRCC 1 cut(s) 2071
BanII GRGCYC 1 cut(s) 2289
BarI GAAGNNNNNNTAC 2 cut(s) 1082, 1114
BauI CACGAG 2 cut(s) 940, 1572
BbsI GAAGAC 2 cut(s) 408, 2325
Bbv12I GWGCWC 3 cut(s) 911, 1328, 1842
BbvI GCAGC 9 cut(s) 224, 227, 502, 630, 781, 1257, 1485, 1657, 1661
BccI CCATC 8 cut(s) 722, 788, 831, 1200, 1289, 2105, 2140, 2212
BceAI ACGGC 3 cut(s) 270, 328, 619
BciT130I CCWGG 3 cut(s) 951, 1164, 1242
BciVI GTATCC 1 cut(s) 2040
BclI TGATCA 1 cut(s) 1636
BcnI CCSGG 5 cut(s) 87, 550, 827, 828, 2165
BcoDI GTCTC 3 cut(s) 99, 523, 2195
BfaI CTAG 5 cut(s) 38, 501, 684, 698, 921
BfmI CTRYAG 2 cut(s) 1002, 2366
BfuI GTATCC 1 cut(s) 2040
BglI GCCNNNNNGGC 1 cut(s) 951
Bme1390I CCNGG 8 cut(s) 87, 550, 827, 828, 951, 1164, 1242, 2165
Bme18I GGWCC 1 cut(s) 354
BmeT110I CYCGRG 3 cut(s) 826, 1041, 1327
BmgBI CACGTC 1 cut(s) 1116
BmgT120I GGNCC 4 cut(s) 354, 385, 605, 893
BmiI GGNNCC 4 cut(s) 289, 1384, 1810, 2073
BmrFI CCNGG 8 cut(s) 87, 550, 827, 828, 951, 1164, 1242, 2165
BmrI ACTGGG 2 cut(s) 382, 988
BmsI GCATC 9 cut(s) 68, 322, 691, 731, 980, 1418, 1455, 2317, 2349
BmuI ACTGGG 2 cut(s) 382, 988
BpiI GAAGAC 2 cut(s) 408, 2325
BplI GAGNNNNNCTC 2 cut(s) 2271, 2303
Bpu14I TTCGAA 2 cut(s) 1222, 1860
BpuEI CTTGAG 1 cut(s) 416
BpuMI CCSGG 5 cut(s) 87, 550, 827, 828, 2165
BsaAI YACGTR 1 cut(s) 912
BsaBI GATNNNNATC 2 cut(s) 798, 1848
BsaHI GRCGYC 1 cut(s) 222
BsaJI CCNNGG 5 cut(s) 638, 826, 1070, 1678, 1812
BsaWI WCCGGW 4 cut(s) 163, 1529, 2074, 2153
BsaXI ACNNNNNCTCC 2 cut(s) 1599, 1629
Bsc4I CCNNNNNNNGG 4 cut(s) 249, 926, 1981, 2171
Bse118I RCCGGY 1 cut(s) 1529
Bse1I ACTGG 3 cut(s) 388, 632, 983
Bse8I GATNNNNATC 2 cut(s) 798, 1848
BseBI CCWGG 3 cut(s) 951, 1164, 1242
BseDI CCNNGG 5 cut(s) 638, 826, 1070, 1678, 1812
BseGI GGATG 7 cut(s) 545, 733, 793, 995, 1281, 1501, 2119
BseJI GATNNNNATC 2 cut(s) 798, 1848
BseLI CCNNNNNNNGG 4 cut(s) 249, 926, 1981, 2171
BseMII CTCAG 1 cut(s) 1809
BseNI ACTGG 3 cut(s) 388, 632, 983
BseSI GKGCMC 1 cut(s) 911
BseXI GCAGC 9 cut(s) 224, 227, 502, 630, 781, 1257, 1485, 1657, 1661
Bsh1236I CGCG 2 cut(s) 264, 1644
Bsh1285I CGRYCG 1 cut(s) 105
BshFI GGCC 6 cut(s) 257, 386, 606, 895, 1191, 2335
BshNI GGYRCC 1 cut(s) 2071
BshTI ACCGGT 1 cut(s) 1529
BsiEI CGRYCG 1 cut(s) 105
BsiHKAI GWGCWC 3 cut(s) 911, 1328, 1842
BsiHKCI CYCGRG 3 cut(s) 826, 1041, 1327
BsiSI CCGG 8 cut(s) 87, 164, 549, 827, 1530, 2075, 2154, 2165
BslFI GGGAC 1 cut(s) 1888
BslI CCNNNNNNNGG 4 cut(s) 249, 926, 1981, 2171
BsmAI GTCTC 3 cut(s) 99, 523, 2195
BsmBI CGTCTC 2 cut(s) 99, 2195
BsmFI GGGAC 1 cut(s) 1888
BsmI GAATGC 2 cut(s) 1672, 1913
BsnI GGCC 6 cut(s) 257, 386, 606, 895, 1191, 2335
BsoBI CYCGRG 3 cut(s) 826, 1041, 1327
Bsp119I TTCGAA 2 cut(s) 1222, 1860
Bsp1286I GDGCHC 4 cut(s) 911, 1328, 1842, 2289
Bsp1407I TGTACA 2 cut(s) 810, 1617
Bsp143I GATC 7 cut(s) 89, 126, 287, 634, 1045, 1636, 1740
BspACI CCGC 6 cut(s) 225, 262, 785, 1700, 1985, 2168
BspANI GGCC 6 cut(s) 257, 386, 606, 895, 1191, 2335
BspCNI CTCAG 1 cut(s) 1810
BspFNI CGCG 2 cut(s) 264, 1644
BspHI TCATGA 1 cut(s) 2289
BspLI GGNNCC 4 cut(s) 289, 1384, 1810, 2073
BspPI GGATC 4 cut(s) 97, 282, 295, 629
BspQI GCTCTTC 1 cut(s) 2307
BspT104I TTCGAA 2 cut(s) 1222, 1860
BspT107I GGYRCC 1 cut(s) 2071
BsrFI RCCGGY 1 cut(s) 1529
BsrGI TGTACA 2 cut(s) 810, 1617
BsrI ACTGG 3 cut(s) 388, 632, 983
BssAI RCCGGY 1 cut(s) 1529
BssECI CCNNGG 5 cut(s) 638, 826, 1070, 1678, 1812
BssMI GATC 7 cut(s) 89, 126, 287, 634, 1045, 1636, 1740
BssNI GRCGYC 1 cut(s) 222
BssSI CACGAG 2 cut(s) 940, 1572
BssT1I CCWWGG 3 cut(s) 638, 1678, 1812
Bst2BI CACGAG 2 cut(s) 940, 1572
Bst2UI CCWGG 3 cut(s) 951, 1164, 1242
Bst4CI ACNGT 8 cut(s) 244, 281, 367, 542, 815, 1160, 1229, 2370
Bst6I CTCTTC 5 cut(s) 526, 769, 1473, 1482, 2307
BstACI GRCGYC 1 cut(s) 222
BstAUI TGTACA 2 cut(s) 810, 1617
BstBAI YACGTR 1 cut(s) 912
BstBI TTCGAA 2 cut(s) 1222, 1860
BstC8I GCNNGC 8 cut(s) 210, 657, 855, 995, 1238, 1346, 1772, 1890
BstDEI CTNAG 3 cut(s) 647, 1144, 1818
BstEII GGTNACC 1 cut(s) 2149
BstF5I GGATG 7 cut(s) 545, 733, 793, 995, 1281, 1501, 2119
BstFNI CGCG 2 cut(s) 264, 1644
BstKTI GATC 7 cut(s) 92, 129, 290, 637, 1048, 1639, 1743
BstMAI GTCTC 3 cut(s) 99, 523, 2195
BstMBI GATC 7 cut(s) 89, 126, 287, 634, 1045, 1636, 1740
BstMCI CGRYCG 1 cut(s) 105
BstMWI GCNNNNNNNGC 6 cut(s) 65, 221, 224, 652, 951, 1767
BstNI CCWGG 3 cut(s) 951, 1164, 1242
BstNSI RCATGY 3 cut(s) 145, 1324, 2095
BstPI GGTNACC 1 cut(s) 2149
BstSCI CCNGG 8 cut(s) 85, 548, 825, 826, 949, 1162, 1240, 2163
BstSFI CTRYAG 2 cut(s) 1002, 2366
BstSLI GKGCMC 1 cut(s) 911
BstUI CGCG 2 cut(s) 264, 1644
BstV1I GCAGC 9 cut(s) 224, 227, 502, 630, 781, 1257, 1485, 1657, 1661
BstV2I GAAGAC 2 cut(s) 408, 2325
BstX2I RGATCY 1 cut(s) 287
BstYI RGATCY 1 cut(s) 287
BsuI GTATCC 1 cut(s) 2040
BsuRI GGCC 6 cut(s) 257, 386, 606, 895, 1191, 2335
BtrI CACGTC 1 cut(s) 1116
BtsCI GGATG 7 cut(s) 545, 733, 793, 995, 1281, 1501, 2119
BtsI GCAGTG 1 cut(s) 699
BtsIMutI CAGTG 3 cut(s) 363, 699, 724
Cac8I GCNNGC 8 cut(s) 210, 657, 855, 995, 1238, 1346, 1772, 1890
CciI TCATGA 1 cut(s) 2289
Cfr10I RCCGGY 1 cut(s) 1529
Cfr13I GGNCC 4 cut(s) 354, 385, 605, 893
Cfr9I CCCGGG 1 cut(s) 826
CseI GACGC 5 cut(s) 57, 230, 1633, 2212, 2353
Csp6I GTAC 8 cut(s) 419, 811, 1035, 1090, 1374, 1618, 1795, 1922
CspAI ACCGGT 1 cut(s) 1529
CviQI GTAC 8 cut(s) 419, 811, 1035, 1090, 1374, 1618, 1795, 1922
DdeI CTNAG 3 cut(s) 647, 1144, 1818
DpnI GATC 7 cut(s) 91, 128, 289, 636, 1047, 1638, 1742
DpnII GATC 7 cut(s) 89, 126, 287, 634, 1045, 1636, 1740
DraI TTTAAA 1 cut(s) 2010
DrdI GACNNNNNNGTC 1 cut(s) 272
DseDI GACNNNNNNGTC 1 cut(s) 272
EaeI YGGCCR 2 cut(s) 255, 1189
Eam1104I CTCTTC 5 cut(s) 526, 769, 1473, 1482, 2307
EarI CTCTTC 5 cut(s) 526, 769, 1473, 1482, 2307
EciI GGCGGA 3 cut(s) 800, 2000, 2183
Eco130I CCWWGG 3 cut(s) 638, 1678, 1812
Eco147I AGGCCT 1 cut(s) 2335
Eco24I GRGCYC 1 cut(s) 2289
Eco47I GGWCC 1 cut(s) 354
Eco57I CTGAAG 1 cut(s) 1286
Eco88I CYCGRG 3 cut(s) 826, 1041, 1327
Eco91I GGTNACC 1 cut(s) 2149
EcoO65I GGTNACC 1 cut(s) 2149
EcoRII CCWGG 3 cut(s) 949, 1162, 1240
EcoT14I CCWWGG 3 cut(s) 638, 1678, 1812
EcoT38I GRGCYC 1 cut(s) 2289
ErhI CCWWGG 3 cut(s) 638, 1678, 1812
Esp3I CGTCTC 2 cut(s) 99, 2195
FalI AAGNNNNNCTT 8 cut(s) 445, 477, 655, 687, 1500, 1532, 2286, 2318
FaqI GGGAC 1 cut(s) 1888
FauNDI CATATG 1 cut(s) 1360
FbaI TGATCA 1 cut(s) 1636
FokI GGATG 7 cut(s) 532, 740, 800, 1002, 1268, 1508, 2126
FriOI GRGCYC 1 cut(s) 2289
FspBI CTAG 5 cut(s) 38, 501, 684, 698, 921
HaeIII GGCC 6 cut(s) 257, 386, 606, 895, 1191, 2335
HapII CCGG 8 cut(s) 87, 164, 549, 827, 1530, 2075, 2154, 2165
HgaI GACGC 5 cut(s) 57, 230, 1633, 2212, 2353
Hin1I GRCGYC 1 cut(s) 222
HincII GTYRAC 2 cut(s) 277, 1111
HindII GTYRAC 2 cut(s) 277, 1111
HindIII AAGCTT 4 cut(s) 454, 512, 1721, 2003
HinfI GANTC 6 cut(s) 1213, 1314, 1490, 1849, 1961, 2083
HpaI GTTAAC 1 cut(s) 1111
HpaII CCGG 8 cut(s) 87, 164, 549, 827, 1530, 2075, 2154, 2165
HphI GGTGA 6 cut(s) 182, 193, 232, 289, 2161, 2242
Hpy166II GTNNAC 7 cut(s) 201, 277, 297, 909, 1111, 1618, 2119
Hpy188III TCNNGA 8 cut(s) 190, 398, 525, 1043, 1353, 1427, 2290, 2375
Hpy8I GTNNAC 7 cut(s) 201, 277, 297, 909, 1111, 1618, 2119
Hpy99I CGWCG 4 cut(s) 224, 248, 605, 1649
HpyCH4III ACNGT 8 cut(s) 244, 281, 367, 542, 815, 1160, 1229, 2370
HpyCH4IV ACGT 3 cut(s) 911, 1107, 1115
HpyF10VI GCNNNNNNNGC 6 cut(s) 65, 221, 224, 652, 951, 1767
HpyF3I CTNAG 3 cut(s) 647, 1144, 1818
HpySE526I ACGT 3 cut(s) 911, 1107, 1115
Hsp92I GRCGYC 1 cut(s) 222
Ksp22I TGATCA 1 cut(s) 1636
KspAI GTTAAC 1 cut(s) 1111
Kzo9I GATC 7 cut(s) 89, 126, 287, 634, 1045, 1636, 1740
LguI GCTCTTC 1 cut(s) 2307
Lsp1109I GCAGC 9 cut(s) 224, 227, 502, 630, 781, 1257, 1485, 1657, 1661
LweI GCATC 9 cut(s) 68, 322, 691, 731, 980, 1418, 1455, 2317, 2349
MaeI CTAG 5 cut(s) 38, 501, 684, 698, 921
MaeII ACGT 3 cut(s) 911, 1107, 1115
MaeIII GTNAC 3 cut(s) 1501, 1525, 2149
MalI GATC 7 cut(s) 91, 128, 289, 636, 1047, 1638, 1742
MboI GATC 7 cut(s) 89, 126, 287, 634, 1045, 1636, 1740
MfeI CAATTG 2 cut(s) 858, 1198
MflI RGATCY 1 cut(s) 287
MhlI GDGCHC 4 cut(s) 911, 1328, 1842, 2289
MlsI TGGCCA 1 cut(s) 1191
MluI ACGCGT 1 cut(s) 1642
MluNI TGGCCA 1 cut(s) 1191
MlyI GAGTC 3 cut(s) 1207, 1499, 1955
MmeI TCCRAC 2 cut(s) 384, 1473
Mox20I TGGCCA 1 cut(s) 1191
MroXI GAANNNNTTC 1 cut(s) 2175
MscI TGGCCA 1 cut(s) 1191
MseI TTAA 4 cut(s) 392, 735, 1110, 2009
MslI CAYNNNNRTG 5 cut(s) 16, 1448, 1932, 2077, 2294
Msp20I TGGCCA 1 cut(s) 1191
MspA1I CMGCKG 2 cut(s) 152, 271
MspI CCGG 8 cut(s) 87, 164, 549, 827, 1530, 2075, 2154, 2165
MspR9I CCNGG 8 cut(s) 87, 550, 827, 828, 951, 1164, 1242, 2165
MunI CAATTG 2 cut(s) 858, 1198
Mva1269I GAATGC 2 cut(s) 1672, 1913
MvaI CCWGG 3 cut(s) 951, 1164, 1242
MvnI CGCG 2 cut(s) 264, 1644
MwoI GCNNNNNNNGC 6 cut(s) 65, 221, 224, 652, 951, 1767
NciI CCSGG 5 cut(s) 87, 550, 827, 828, 2165
NdeI CATATG 1 cut(s) 1360
NdeII GATC 7 cut(s) 89, 126, 287, 634, 1045, 1636, 1740
NlaIV GGNNCC 4 cut(s) 289, 1384, 1810, 2073
NmuCI GTSAC 1 cut(s) 2149
NspI RCATGY 3 cut(s) 145, 1324, 2095
NspV TTCGAA 2 cut(s) 1222, 1860
OliI CACNNNNGTG 1 cut(s) 2077
PaeR7I CTCGAG 2 cut(s) 1041, 1327
PagI TCATGA 1 cut(s) 2289
PceI AGGCCT 1 cut(s) 2335
PciI ACATGT 1 cut(s) 1320
PciSI GCTCTTC 1 cut(s) 2307
PcsI WCGNNNNNNNCGW 1 cut(s) 2039
PctI GAATGC 2 cut(s) 1672, 1913
PdmI GAANNNNTTC 1 cut(s) 2175
PfeI GAWTC 3 cut(s) 1314, 1849, 2083
PinAI ACCGGT 1 cut(s) 1529
PleI GAGTC 3 cut(s) 1207, 1498, 1955
PpsI GAGTC 3 cut(s) 1207, 1498, 1955
Ppu21I YACGTR 1 cut(s) 912
PscI ACATGT 1 cut(s) 1320
Psp6I CCWGG 3 cut(s) 949, 1162, 1240
PspEI GGTNACC 1 cut(s) 2149
PspGI CCWGG 3 cut(s) 949, 1162, 1240
PspN4I GGNNCC 4 cut(s) 289, 1384, 1810, 2073
PspPI GGNCC 4 cut(s) 354, 385, 605, 893
PspXI VCTCGAGB 1 cut(s) 1327
PsrI GAACNNNNNNTAC 2 cut(s) 1366, 1398
PsuI RGATCY 1 cut(s) 287
PvuII CAGCTG 2 cut(s) 152, 271
RsaI GTAC 8 cut(s) 420, 812, 1036, 1091, 1375, 1619, 1796, 1923
RsaNI GTAC 8 cut(s) 419, 811, 1035, 1090, 1374, 1618, 1795, 1922
RseI CAYNNNNRTG 5 cut(s) 16, 1448, 1932, 2077, 2294
SapI GCTCTTC 1 cut(s) 2307
SaqAI TTAA 4 cut(s) 392, 735, 1110, 2009
Sau3AI GATC 7 cut(s) 89, 126, 287, 634, 1045, 1636, 1740
Sau96I GGNCC 4 cut(s) 354, 385, 605, 893
SchI GAGTC 3 cut(s) 1207, 1499, 1955
ScrFI CCNGG 8 cut(s) 87, 550, 827, 828, 951, 1164, 1242, 2165
SduI GDGCHC 4 cut(s) 911, 1328, 1842, 2289
SfaNI GCATC 9 cut(s) 68, 322, 691, 731, 980, 1418, 1455, 2317, 2349
SfcI CTRYAG 2 cut(s) 1002, 2366
Sfr274I CTCGAG 2 cut(s) 1041, 1327
SfuI TTCGAA 2 cut(s) 1222, 1860
SinI GGWCC 1 cut(s) 354
SlaI CTCGAG 2 cut(s) 1041, 1327
SmaI CCCGGG 1 cut(s) 828
SmiMI CAYNNNNRTG 5 cut(s) 16, 1448, 1932, 2077, 2294
SmlI CTYRAG 3 cut(s) 431, 1041, 1327
SmoI CTYRAG 3 cut(s) 431, 1041, 1327
SseBI AGGCCT 1 cut(s) 2335
SsiI CCGC 6 cut(s) 225, 262, 785, 1700, 1985, 2168
SspI AATATT 1 cut(s) 1082
SspMI CTAG 5 cut(s) 38, 501, 684, 698, 921
StuI AGGCCT 1 cut(s) 2335
StyD4I CCNGG 8 cut(s) 85, 548, 825, 826, 949, 1162, 1240, 2163
StyI CCWWGG 3 cut(s) 638, 1678, 1812
TaaI ACNGT 8 cut(s) 244, 281, 367, 542, 815, 1160, 1229, 2370
TaiI ACGT 3 cut(s) 914, 1110, 1118
TaqII GACCGA 1 cut(s) 91
TatI WGTACW 6 cut(s) 418, 810, 1034, 1373, 1617, 1794
TauI GCSGC 1 cut(s) 227
TfiI GAWTC 3 cut(s) 1314, 1849, 2083
Tru1I TTAA 4 cut(s) 392, 735, 1110, 2009
Tru9I TTAA 4 cut(s) 392, 735, 1110, 2009
TscAI CASTG 3 cut(s) 370, 699, 724
TseFI GTSAC 1 cut(s) 2149
TseI GCWGC 9 cut(s) 212, 215, 490, 643, 769, 1270, 1473, 1649, 1670
Tsp45I GTSAC 1 cut(s) 2149
TspDTI ATGAA 9 cut(s) 534, 837, 869, 1090, 1508, 1748, 1814, 2258, 2306
TspGWI ACGGA 3 cut(s) 423, 1375, 2262
TspMI CCCGGG 1 cut(s) 826
TspRI CASTG 3 cut(s) 370, 699, 724
VneI GTGCAC 1 cut(s) 907
VpaK11BI GGWCC 1 cut(s) 354
XapI RAATTY 4 cut(s) 109, 712, 876, 1403
XceI RCATGY 3 cut(s) 145, 1324, 2095
XcmI CCANNNNNNNNNTGG 1 cut(s) 1178
XhoI CTCGAG 2 cut(s) 1041, 1327
XmaI CCCGGG 1 cut(s) 826
XmnI GAANNNNTTC 1 cut(s) 2175
XspI CTAG 5 cut(s) 38, 501, 684, 698, 921
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.