FvH4_3g11450

60S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0002131
Physical Location & Seq
Forward (+)
6742881 .. 6743321
441 bp
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UTR
Exon/CDS
Intron
FvH4_3g11450.t1

Sequence Viewer

Length: 441 bp
ATGACGACCAGTTTGAAGAAGAACCGAAAGAAGAGAGGCCACGTCAGCGCCGGCCACGGCCGTATCGGGAAGCACCGCAAGCACCCCGGCGGCAGAGGAAACGCTGGAGGGATGCACCACCACCGGATCCTGTTCGACAAGTACCACCCGGGGTATTTTGGTAAAGTTGGGATGAGATACTTCCACAAGCTTCGCAACAAGTTCTACTGCCCCATCGTCAACGTCGACAAGCTCTGGTCCTTGGTGCCGCAGGAGGTCAAGGACAAGGCCTCCAAAAACGACGTGCCGTTGATTGACGTGACGCAGCACGGGTACTTCAAGATTTTGGGTAAGGGGAAGCTGCCGGCGGACCGGCCTGTGGTGGTGAAGGCTAAGCTTATTTCGAAGGTTGCGGAGAAGAAGATCAAGGAGGCCGGCGGCGCTGTGGTTCTCACCGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L27A

Protein Analysis

147

Amino Acids

16.32

Weight (kDa)

10.46

Isoelectric Point (pI)

21.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L27A PF00828 72 - 143 2.1e-16 Ribosomal proteins 50S-L15, 50S-L18e, 60S-L27A
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 244
AccI GTMKAC 1 cut(s) 225
AciI CCGC 7 cut(s) 76, 90, 248, 347, 392, 417, 435
AclWI GGATC 2 cut(s) 121, 134
AcoI YGGCCR 2 cut(s) 52, 58
AfaI GTAC 2 cut(s) 143, 314
AfiI CCNNNNNNNGG 1 cut(s) 358
AgsI TTSAA 2 cut(s) 16, 319
AjiI CACGTC 3 cut(s) 43, 283, 298
AluBI AGCT 4 cut(s) 190, 232, 340, 376
AluI AGCT 4 cut(s) 190, 232, 340, 376
AlwI GGATC 2 cut(s) 121, 134
Ama87I CYCGRG 1 cut(s) 148
AoxI GGCC 6 cut(s) 37, 52, 58, 267, 353, 411
ApeKI GCWGC 2 cut(s) 304, 340
ArsI GACNNNNNNTTYG 1 cut(s) 27
AspLEI GCGC 2 cut(s) 50, 422
AspS9I GGNCC 2 cut(s) 237, 349
AsuC2I CCSGG 3 cut(s) 87, 149, 150
AsuHPI GGTGA 2 cut(s) 376, 424
AsuII TTCGAA 1 cut(s) 383
AvaI CYCGRG 1 cut(s) 148
AvaII GGWCC 2 cut(s) 237, 349
BaeI ACNNNNGTAYC 2 cut(s) 46, 79
BamHI GGATCC 1 cut(s) 126
BanI GGYRCC 1 cut(s) 244
BbvI GCAGC 2 cut(s) 316, 327
BccI CCATC 1 cut(s) 221
BceAI ACGGC 3 cut(s) 45, 73, 271
BcnI CCSGG 3 cut(s) 87, 149, 150
BfaI CTAG 1 cut(s) 439
BfoI RGCGCY 2 cut(s) 51, 423
BisI GCNGC 5 cut(s) 91, 248, 305, 341, 418
BlpI GCTNAGC 1 cut(s) 372
BlsI GCNGC 5 cut(s) 92, 249, 306, 342, 419
Bme1390I CCNGG 3 cut(s) 87, 149, 150
Bme18I GGWCC 2 cut(s) 237, 349
BmeT110I CYCGRG 1 cut(s) 148
BmgBI CACGTC 3 cut(s) 43, 283, 298
BmgT120I GGNCC 2 cut(s) 237, 349
BmiI GGNNCC 2 cut(s) 128, 246
BmrFI CCNGG 3 cut(s) 87, 149, 150
BmsI GCATC 1 cut(s) 102
BpmI CTGGAG 1 cut(s) 126
Bpu1102I GCTNAGC 1 cut(s) 372
Bpu14I TTCGAA 1 cut(s) 383
BpuMI CCSGG 3 cut(s) 87, 149, 150
BsaJI CCNNGG 5 cut(s) 55, 85, 148, 149, 240
BsaWI WCCGGW 1 cut(s) 123
BsaXI ACNNNNNCTCC 2 cut(s) 254, 284
Bsc4I CCNNNNNNNGG 1 cut(s) 358
Bse118I RCCGGY 4 cut(s) 50, 343, 351, 413
Bse1I ACTGG 1 cut(s) 9
BseDI CCNNGG 5 cut(s) 55, 85, 148, 149, 240
BseGI GGATG 2 cut(s) 117, 177
BseLI CCNNNNNNNGG 1 cut(s) 358
BseNI ACTGG 1 cut(s) 9
BseX3I CGGCCG 1 cut(s) 58
BseXI GCAGC 2 cut(s) 316, 327
Bsh1285I CGRYCG 1 cut(s) 61
BshFI GGCC 6 cut(s) 39, 54, 60, 269, 355, 413
BshNI GGYRCC 1 cut(s) 244
BsiEI CGRYCG 1 cut(s) 61
BsiHKCI CYCGRG 1 cut(s) 148
BsiSI CCGG 7 cut(s) 51, 87, 124, 149, 344, 352, 414
BslI CCNNNNNNNGG 1 cut(s) 358
BsnI GGCC 6 cut(s) 39, 54, 60, 269, 355, 413
BsoBI CYCGRG 1 cut(s) 148
Bsp119I TTCGAA 1 cut(s) 383
Bsp143I GATC 2 cut(s) 126, 402
Bsp1720I GCTNAGC 1 cut(s) 372
BspACI CCGC 7 cut(s) 76, 90, 248, 347, 392, 417, 435
BspANI GGCC 6 cut(s) 39, 54, 60, 269, 355, 413
BspLI GGNNCC 2 cut(s) 128, 246
BspPI GGATC 2 cut(s) 121, 134
BspT104I TTCGAA 1 cut(s) 383
BspT107I GGYRCC 1 cut(s) 244
BsrFI RCCGGY 4 cut(s) 50, 343, 351, 413
BsrI ACTGG 1 cut(s) 9
BssAI RCCGGY 4 cut(s) 50, 343, 351, 413
BssECI CCNNGG 5 cut(s) 55, 85, 148, 149, 240
BssMI GATC 2 cut(s) 126, 402
BssT1I CCWWGG 1 cut(s) 240
Bst6I CTCTTC 1 cut(s) 26
BstBI TTCGAA 1 cut(s) 383
BstC8I GCNNGC 4 cut(s) 52, 80, 345, 415
BstDEI CTNAG 1 cut(s) 372
BstDSI CCRYGG 1 cut(s) 55
BstF5I GGATG 2 cut(s) 117, 177
BstH2I RGCGCY 2 cut(s) 51, 423
BstHHI GCGC 2 cut(s) 50, 422
BstKTI GATC 2 cut(s) 129, 405
BstMBI GATC 2 cut(s) 126, 402
BstMCI CGRYCG 1 cut(s) 61
BstMWI GCNNNNNNNGC 3 cut(s) 45, 79, 419
BstSCI CCNGG 3 cut(s) 85, 147, 148
BstV1I GCAGC 2 cut(s) 316, 327
BstX2I RGATCY 1 cut(s) 126
BstYI RGATCY 1 cut(s) 126
BstZI CGGCCG 1 cut(s) 58
BsuRI GGCC 6 cut(s) 39, 54, 60, 269, 355, 413
BtgI CCRYGG 1 cut(s) 55
BtrI CACGTC 3 cut(s) 43, 283, 298
BtsCI GGATG 2 cut(s) 117, 177
Cac8I GCNNGC 4 cut(s) 52, 80, 345, 415
CfoI GCGC 2 cut(s) 50, 422
Cfr10I RCCGGY 4 cut(s) 50, 343, 351, 413
Cfr13I GGNCC 2 cut(s) 237, 349
Cfr9I CCCGGG 1 cut(s) 148
CpoI CGGWCCG 1 cut(s) 349
CseI GACGC 1 cut(s) 310
Csp6I GTAC 2 cut(s) 142, 313
CspI CGGWCCG 1 cut(s) 349
CviQI GTAC 2 cut(s) 142, 313
DdeI CTNAG 1 cut(s) 372
DpnI GATC 2 cut(s) 128, 404
DpnII GATC 2 cut(s) 126, 402
EaeI YGGCCR 2 cut(s) 52, 58
EagI CGGCCG 1 cut(s) 58
Eam1104I CTCTTC 1 cut(s) 26
EarI CTCTTC 1 cut(s) 26
EciI GGCGGA 1 cut(s) 362
EclXI CGGCCG 1 cut(s) 58
Eco130I CCWWGG 1 cut(s) 240
Eco147I AGGCCT 1 cut(s) 269
Eco47I GGWCC 2 cut(s) 237, 349
Eco52I CGGCCG 1 cut(s) 58
Eco88I CYCGRG 1 cut(s) 148
EcoT14I CCWWGG 1 cut(s) 240
ErhI CCWWGG 1 cut(s) 240
FblI GTMKAC 1 cut(s) 225
Fnu4HI GCNGC 5 cut(s) 91, 248, 305, 341, 418
FokI GGATG 2 cut(s) 124, 184
Fsp4HI GCNGC 5 cut(s) 91, 248, 305, 341, 418
FspBI CTAG 1 cut(s) 439
GlaI GCGC 2 cut(s) 49, 421
GluI GCNGC 5 cut(s) 91, 248, 305, 341, 418
GsuI CTGGAG 1 cut(s) 126
HaeII RGCGCY 2 cut(s) 51, 423
HaeIII GGCC 6 cut(s) 39, 54, 60, 269, 355, 413
HapII CCGG 7 cut(s) 51, 87, 124, 149, 344, 352, 414
HgaI GACGC 1 cut(s) 310
HhaI GCGC 2 cut(s) 50, 422
Hin6I GCGC 2 cut(s) 48, 420
HinP1I GCGC 2 cut(s) 48, 420
HincII GTYRAC 2 cut(s) 220, 226
HindII GTYRAC 2 cut(s) 220, 226
HindIII AAGCTT 2 cut(s) 188, 374
HpaII CCGG 7 cut(s) 51, 87, 124, 149, 344, 352, 414
HphI GGTGA 2 cut(s) 376, 424
Hpy166II GTNNAC 2 cut(s) 220, 226
Hpy188III TCNNGA 2 cut(s) 67, 319
Hpy8I GTNNAC 2 cut(s) 220, 226
Hpy99I CGWCG 2 cut(s) 227, 284
HpyAV CCTTC 2 cut(s) 361, 379
HpyCH4IV ACGT 4 cut(s) 42, 222, 282, 297
HpyCH4V TGCA 1 cut(s) 115
HpyF10VI GCNNNNNNNGC 3 cut(s) 45, 79, 419
HpyF3I CTNAG 1 cut(s) 372
HpySE526I ACGT 4 cut(s) 42, 222, 282, 297
HspAI GCGC 2 cut(s) 48, 420
KroI GCCGGC 3 cut(s) 50, 343, 413
KroNI GCCGGC 3 cut(s) 52, 345, 415
Kzo9I GATC 2 cut(s) 126, 402
Lsp1109I GCAGC 2 cut(s) 316, 327
LweI GCATC 1 cut(s) 102
MaeI CTAG 1 cut(s) 439
MaeII ACGT 4 cut(s) 42, 222, 282, 297
MaeIII GTNAC 1 cut(s) 298
MalI GATC 2 cut(s) 128, 404
MboI GATC 2 cut(s) 126, 402
MboII GAAGA 5 cut(s) 28, 31, 43, 409, 412
MflI RGATCY 1 cut(s) 126
MnlI CCTC 6 cut(s) 29, 89, 101, 247, 280, 403
MroNI GCCGGC 3 cut(s) 50, 343, 413
MspI CCGG 7 cut(s) 51, 87, 124, 149, 344, 352, 414
MspR9I CCNGG 3 cut(s) 87, 149, 150
MwoI GCNNNNNNNGC 3 cut(s) 45, 79, 419
NaeI GCCGGC 3 cut(s) 52, 345, 415
NciI CCSGG 3 cut(s) 87, 149, 150
NdeII GATC 2 cut(s) 126, 402
NgoMIV GCCGGC 3 cut(s) 50, 343, 413
NlaIV GGNNCC 2 cut(s) 128, 246
NmuCI GTSAC 1 cut(s) 298
NspV TTCGAA 1 cut(s) 383
PceI AGGCCT 1 cut(s) 269
PcsI WCGNNNNNNNCGW 1 cut(s) 222
PdiI GCCGGC 3 cut(s) 52, 345, 415
PkrI GCNGC 5 cut(s) 92, 249, 306, 342, 419
PspN4I GGNNCC 2 cut(s) 128, 246
PspPI GGNCC 2 cut(s) 237, 349
PsuI RGATCY 1 cut(s) 126
RsaI GTAC 2 cut(s) 143, 314
RsaNI GTAC 2 cut(s) 142, 313
Rsr2I CGGWCCG 1 cut(s) 349
RsrII CGGWCCG 1 cut(s) 349
SalI GTCGAC 1 cut(s) 224
SatI GCNGC 5 cut(s) 91, 248, 305, 341, 418
Sau3AI GATC 2 cut(s) 126, 402
Sau96I GGNCC 2 cut(s) 237, 349
ScrFI CCNGG 3 cut(s) 87, 149, 150
SfaNI GCATC 1 cut(s) 102
SfuI TTCGAA 1 cut(s) 383
SinI GGWCC 2 cut(s) 237, 349
SmaI CCCGGG 1 cut(s) 150
SseBI AGGCCT 1 cut(s) 269
SsiI CCGC 7 cut(s) 76, 90, 248, 347, 392, 417, 435
SspMI CTAG 1 cut(s) 439
StuI AGGCCT 1 cut(s) 269
StyD4I CCNGG 3 cut(s) 85, 147, 148
StyI CCWWGG 1 cut(s) 240
TaiI ACGT 4 cut(s) 45, 225, 285, 300
TaqI TCGA 3 cut(s) 135, 225, 383
TauI GCSGC 3 cut(s) 93, 250, 420
TseFI GTSAC 1 cut(s) 298
TseI GCWGC 2 cut(s) 304, 340
Tsp45I GTSAC 1 cut(s) 298
TspMI CCCGGG 1 cut(s) 148
VpaK11BI GGWCC 2 cut(s) 237, 349
XmaI CCCGGG 1 cut(s) 148
XmiI GTMKAC 1 cut(s) 225
XspI CTAG 1 cut(s) 439
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.