RLG00000032359

60S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0002131
Physical Location & Seq
Forward (+)
13948573 .. 13951036
2464 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000032359

Sequence Viewer

Length: 441 bp
ATGACGACCCGGTTGAAGAAAAACCGAAAGAAAAGAGGCCACGTCAGCGCCGGTCACGGCCGGATCGGGAAGCACCGGAAGCACCCCGGCGGGCGGGGAAACGCCGGAGGGATGCACCACCACCGGATCCTGTTCGACAAGTACCACCCGGGGTATTTCGGTAAAGTGGGCATGAGGTACTTCCACAAGCTCCGCAACAAGTTCTACTGCCCCGTCGTCAACGTCGACAGGCTCTGGTCGCTGGTGCCGCAGGAGGTCAAGGACAAGGCCACCAAAAACGACGTGCCGTTGATTGACGCGACGCAGCATGGGTTCTTCAAGATTTTGGGCAAGGGGAAGCTGCCGGCGGACCGGCCCGTGGTGGTGAAGGCCAAGCTCATTTCGAAGGTCGCCGAGAAGAAGATCAAGGAGGCCGGCGGCGCCGTGGTTCTCACCGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L27A

Protein Analysis

147

Amino Acids

16.38

Weight (kDa)

10.68

Isoelectric Point (pI)

24.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L27A PF00828 72 - 143 5.6e-16 Ribosomal proteins 50S-L15, 50S-L18e, 60S-L27A
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 244, 419
AccI GTMKAC 1 cut(s) 225
AccII CGCG 1 cut(s) 299
AciI CCGC 7 cut(s) 90, 94, 193, 248, 347, 417, 435
AclWI GGATC 3 cut(s) 71, 121, 134
AcoI YGGCCR 1 cut(s) 58
AcyI GRCGYC 1 cut(s) 420
AfaI GTAC 2 cut(s) 143, 179
AfiI CCNNNNNNNGG 2 cut(s) 93, 358
AgsI TTSAA 2 cut(s) 16, 319
AjiI CACGTC 2 cut(s) 43, 283
AluBI AGCT 3 cut(s) 190, 340, 376
AluI AGCT 3 cut(s) 190, 340, 376
AlwI GGATC 3 cut(s) 71, 121, 134
AlwNI CAGNNNCTG 1 cut(s) 234
Ama87I CYCGRG 1 cut(s) 148
AoxI GGCC 6 cut(s) 37, 58, 267, 353, 369, 411
ApeKI GCWGC 2 cut(s) 304, 340
AspLEI GCGC 2 cut(s) 50, 422
AspS9I GGNCC 2 cut(s) 349, 354
AsuC2I CCSGG 4 cut(s) 10, 87, 149, 150
AsuHPI GGTGA 2 cut(s) 376, 424
AsuII TTCGAA 1 cut(s) 383
AvaI CYCGRG 1 cut(s) 148
AvaII GGWCC 1 cut(s) 349
BamHI GGATCC 1 cut(s) 126
BanI GGYRCC 2 cut(s) 244, 419
BbvI GCAGC 2 cut(s) 316, 327
BceAI ACGGC 3 cut(s) 73, 271, 407
BcnI CCSGG 4 cut(s) 10, 87, 149, 150
BfaI CTAG 1 cut(s) 439
BfoI RGCGCY 2 cut(s) 51, 423
BisI GCNGC 4 cut(s) 248, 305, 341, 418
BlsI GCNGC 4 cut(s) 249, 306, 342, 419
Bme1390I CCNGG 4 cut(s) 10, 87, 149, 150
Bme18I GGWCC 1 cut(s) 349
BmeT110I CYCGRG 1 cut(s) 148
BmgBI CACGTC 2 cut(s) 43, 283
BmgT120I GGNCC 2 cut(s) 349, 354
BmiI GGNNCC 3 cut(s) 128, 246, 421
BmrFI CCNGG 4 cut(s) 10, 87, 149, 150
BmsI GCATC 1 cut(s) 102
Bpu14I TTCGAA 1 cut(s) 383
BpuMI CCSGG 4 cut(s) 10, 87, 149, 150
BsaHI GRCGYC 1 cut(s) 420
BsaJI CCNNGG 5 cut(s) 85, 148, 149, 357, 423
BsaWI WCCGGW 2 cut(s) 75, 123
Bsc4I CCNNNNNNNGG 2 cut(s) 93, 358
Bse118I RCCGGY 4 cut(s) 50, 343, 351, 413
BseDI CCNNGG 5 cut(s) 85, 148, 149, 357, 423
BseGI GGATG 1 cut(s) 117
BseLI CCNNNNNNNGG 2 cut(s) 93, 358
BseX3I CGGCCG 1 cut(s) 58
BseXI GCAGC 2 cut(s) 316, 327
Bsh1236I CGCG 1 cut(s) 299
Bsh1285I CGRYCG 1 cut(s) 61
BshFI GGCC 6 cut(s) 39, 60, 269, 355, 371, 413
BshNI GGYRCC 2 cut(s) 244, 419
BsiEI CGRYCG 1 cut(s) 61
BsiHKCI CYCGRG 1 cut(s) 148
BslI CCNNNNNNNGG 2 cut(s) 93, 358
BsnI GGCC 6 cut(s) 39, 60, 269, 355, 371, 413
BsoBI CYCGRG 1 cut(s) 148
Bsp119I TTCGAA 1 cut(s) 383
Bsp143I GATC 3 cut(s) 63, 126, 402
BspACI CCGC 7 cut(s) 90, 94, 193, 248, 347, 417, 435
BspANI GGCC 6 cut(s) 39, 60, 269, 355, 371, 413
BspFNI CGCG 1 cut(s) 299
BspLI GGNNCC 3 cut(s) 128, 246, 421
BspPI GGATC 3 cut(s) 71, 121, 134
BspT104I TTCGAA 1 cut(s) 383
BspT107I GGYRCC 2 cut(s) 244, 419
BsrFI RCCGGY 4 cut(s) 50, 343, 351, 413
BssAI RCCGGY 4 cut(s) 50, 343, 351, 413
BssECI CCNNGG 5 cut(s) 85, 148, 149, 357, 423
BssMI GATC 3 cut(s) 63, 126, 402
BssNI GRCGYC 1 cut(s) 420
BstACI GRCGYC 1 cut(s) 420
BstBI TTCGAA 1 cut(s) 383
BstC8I GCNNGC 3 cut(s) 92, 345, 415
BstDSI CCRYGG 2 cut(s) 357, 423
BstF5I GGATG 1 cut(s) 117
BstFNI CGCG 1 cut(s) 299
BstH2I RGCGCY 2 cut(s) 51, 423
BstHHI GCGC 2 cut(s) 50, 422
BstKTI GATC 3 cut(s) 66, 129, 405
BstMBI GATC 3 cut(s) 63, 126, 402
BstMCI CGRYCG 1 cut(s) 61
BstMWI GCNNNNNNNGC 5 cut(s) 45, 79, 238, 247, 419
BstSCI CCNGG 4 cut(s) 8, 85, 147, 148
BstUI CGCG 1 cut(s) 299
BstV1I GCAGC 2 cut(s) 316, 327
BstX2I RGATCY 1 cut(s) 126
BstYI RGATCY 1 cut(s) 126
BstZI CGGCCG 1 cut(s) 58
BsuRI GGCC 6 cut(s) 39, 60, 269, 355, 371, 413
BtgI CCRYGG 2 cut(s) 357, 423
BtrI CACGTC 2 cut(s) 43, 283
BtsCI GGATG 1 cut(s) 117
Cac8I GCNNGC 3 cut(s) 92, 345, 415
CaiI CAGNNNCTG 1 cut(s) 234
CfoI GCGC 2 cut(s) 50, 422
Cfr10I RCCGGY 4 cut(s) 50, 343, 351, 413
Cfr13I GGNCC 2 cut(s) 349, 354
Cfr9I CCCGGG 1 cut(s) 148
CpoI CGGWCCG 1 cut(s) 349
CseI GACGC 2 cut(s) 305, 310
Csp6I GTAC 2 cut(s) 142, 178
CspI CGGWCCG 1 cut(s) 349
CviAII CATG 2 cut(s) 172, 308
CviQI GTAC 2 cut(s) 142, 178
DinI GGCGCC 1 cut(s) 421
DpnI GATC 3 cut(s) 65, 128, 404
DpnII GATC 3 cut(s) 63, 126, 402
EaeI YGGCCR 1 cut(s) 58
EagI CGGCCG 1 cut(s) 58
EciI GGCGGA 1 cut(s) 362
EclXI CGGCCG 1 cut(s) 58
Eco47I GGWCC 1 cut(s) 349
Eco52I CGGCCG 1 cut(s) 58
Eco88I CYCGRG 1 cut(s) 148
EgeI GGCGCC 1 cut(s) 421
EheI GGCGCC 1 cut(s) 421
FaeI CATG 2 cut(s) 175, 311
FaiI YATR 2 cut(s) 173, 309
FatI CATG 2 cut(s) 171, 307
FauI CCCGC 2 cut(s) 83, 87
FblI GTMKAC 1 cut(s) 225
Fnu4HI GCNGC 4 cut(s) 248, 305, 341, 418
FokI GGATG 1 cut(s) 124
Fsp4HI GCNGC 4 cut(s) 248, 305, 341, 418
FspBI CTAG 1 cut(s) 439
GlaI GCGC 2 cut(s) 49, 421
GluI GCNGC 4 cut(s) 248, 305, 341, 418
HaeII RGCGCY 2 cut(s) 51, 423
HaeIII GGCC 6 cut(s) 39, 60, 269, 355, 371, 413
HgaI GACGC 2 cut(s) 305, 310
HhaI GCGC 2 cut(s) 50, 422
Hin1I GRCGYC 1 cut(s) 420
Hin1II CATG 2 cut(s) 175, 311
Hin6I GCGC 2 cut(s) 48, 420
HinP1I GCGC 2 cut(s) 48, 420
HincII GTYRAC 2 cut(s) 220, 226
HindII GTYRAC 2 cut(s) 220, 226
HphI GGTGA 2 cut(s) 376, 424
Hpy166II GTNNAC 2 cut(s) 220, 226
Hpy188III TCNNGA 2 cut(s) 67, 319
Hpy8I GTNNAC 2 cut(s) 220, 226
Hpy99I CGWCG 4 cut(s) 218, 227, 284, 304
HpyAV CCTTC 2 cut(s) 361, 379
HpyCH4IV ACGT 3 cut(s) 42, 222, 282
HpyCH4V TGCA 1 cut(s) 115
HpyF10VI GCNNNNNNNGC 5 cut(s) 45, 79, 238, 247, 419
HpySE526I ACGT 3 cut(s) 42, 222, 282
Hsp92I GRCGYC 1 cut(s) 420
Hsp92II CATG 2 cut(s) 175, 311
HspAI GCGC 2 cut(s) 48, 420
KasI GGCGCC 1 cut(s) 419
KroI GCCGGC 2 cut(s) 343, 413
KroNI GCCGGC 2 cut(s) 345, 415
Kzo9I GATC 3 cut(s) 63, 126, 402
LmnI GCTCC 1 cut(s) 195
Lsp1109I GCAGC 2 cut(s) 316, 327
LweI GCATC 1 cut(s) 102
MaeI CTAG 1 cut(s) 439
MaeII ACGT 3 cut(s) 42, 222, 282
MaeIII GTNAC 1 cut(s) 53
MalI GATC 3 cut(s) 65, 128, 404
MboI GATC 3 cut(s) 63, 126, 402
MboII GAAGA 4 cut(s) 28, 307, 409, 412
MflI RGATCY 1 cut(s) 126
Mly113I GGCGCC 1 cut(s) 420
MnlI CCTC 5 cut(s) 29, 101, 168, 247, 403
MroNI GCCGGC 2 cut(s) 343, 413
MspR9I CCNGG 4 cut(s) 10, 87, 149, 150
MvnI CGCG 1 cut(s) 299
MwoI GCNNNNNNNGC 5 cut(s) 45, 79, 238, 247, 419
NaeI GCCGGC 2 cut(s) 345, 415
NarI GGCGCC 1 cut(s) 420
NciI CCSGG 4 cut(s) 10, 87, 149, 150
NdeII GATC 3 cut(s) 63, 126, 402
NgoMIV GCCGGC 2 cut(s) 343, 413
NlaIII CATG 2 cut(s) 175, 311
NlaIV GGNNCC 3 cut(s) 128, 246, 421
NmeAIII GCCGAG 1 cut(s) 418
NmuCI GTSAC 1 cut(s) 53
NspV TTCGAA 1 cut(s) 383
PcsI WCGNNNNNNNCGW 1 cut(s) 222
PdiI GCCGGC 2 cut(s) 345, 415
PkrI GCNGC 4 cut(s) 249, 306, 342, 419
PluTI GGCGCC 1 cut(s) 423
PspN4I GGNNCC 3 cut(s) 128, 246, 421
PspPI GGNCC 2 cut(s) 349, 354
PstNI CAGNNNCTG 1 cut(s) 234
PsuI RGATCY 1 cut(s) 126
RsaI GTAC 2 cut(s) 143, 179
RsaNI GTAC 2 cut(s) 142, 178
Rsr2I CGGWCCG 1 cut(s) 349
RsrII CGGWCCG 1 cut(s) 349
SalI GTCGAC 1 cut(s) 224
SatI GCNGC 4 cut(s) 248, 305, 341, 418
Sau3AI GATC 3 cut(s) 63, 126, 402
Sau96I GGNCC 2 cut(s) 349, 354
ScrFI CCNGG 4 cut(s) 10, 87, 149, 150
SetI ASST 9 cut(s) 45, 179, 192, 225, 258, 285, 342, 378, 390
SfaNI GCATC 1 cut(s) 102
SfoI GGCGCC 1 cut(s) 421
SfuI TTCGAA 1 cut(s) 383
SinI GGWCC 1 cut(s) 349
SmaI CCCGGG 1 cut(s) 150
SsiI CCGC 7 cut(s) 90, 94, 193, 248, 347, 417, 435
SspDI GGCGCC 1 cut(s) 419
SspMI CTAG 1 cut(s) 439
StyD4I CCNGG 4 cut(s) 8, 85, 147, 148
TaiI ACGT 3 cut(s) 45, 225, 285
TaqI TCGA 3 cut(s) 135, 225, 383
TauI GCSGC 2 cut(s) 250, 420
TseFI GTSAC 1 cut(s) 53
TseI GCWGC 2 cut(s) 304, 340
Tsp45I GTSAC 1 cut(s) 53
TspMI CCCGGG 1 cut(s) 148
VpaK11BI GGWCC 1 cut(s) 349
XmaI CCCGGG 1 cut(s) 148
XmiI GTMKAC 1 cut(s) 225
XspI CTAG 1 cut(s) 439
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.